- Email: [email protected]
The effect of short-term topical tretinoin on photodamaged skin
137 167
166
SUBCELLULAR PHARMACOKINETICSOF ‘OB1-DOPA ANALOGUE AS MELANOMA-SEEKING COMPOUND FOR BORON NEUTRON CAPTURE THERAPY M SHIONO’, C. HONDA’, M. ICHIHASHI’, T. SHIBATAs, Y. MISHIMA’~3 and B. KAGEDAL‘$ ‘Dept. of Derm., Kobe Uw. Sch. of Med., *Kobe Karser Hosprtal, 3Mrshima Inst. for Derm. Res Kobe Japan, “Dept. of Clrnrcal Chem The Univ of Lrnkopfng, Linkbping, Sweden
PLASMADEPENDENT CELLULAR CYTOTOXICITY: ANOVELTUMORCIDAL ACTIVITY OF THE MOfiOCYTIC CELL LIREAGE
10B1-paraboronophenylalanrne(loBt-BPA), dopa analogues synthesized and used capture
therapy(NCT).
has
a
one of the boronated by
us
selective
for thermal
affinity for
neutron
mallgnant
We have already successfully treated human melanoma cases by NCT usrng this compound In this study. we investigate the conhnuous In viva mrcrodralysts assay of “‘B-dynamics wrthrn rntercellular flurd of melanoma and normal subcutaneous t&sue We also report ‘OB concentratron and distribution dynamrcs withrn melanoma cells In the light of subcellular fractron analysis of excused melanoma tissue In order to elucrdate the mechamsm of the selectrve affrnrty of ‘061.BPA for malrgnant melanoma melanoma.
S .SHIRAHAMA’ , D.P.DIALYNAS2, T.S. EDGINGTON 'Dept. of Dermatol. Hamamatsu Univ. Sch. of Med., 3600 Ilanda-cho, Hamamatsu 431-31. Japan. 2Research Inst. of Scripps Clinic, L’SA. Cellular cytotoxicity mediated by cells of monocytes (no) lineaee. particulalv that with tumorcidal orooertics. has been kxtekively inkstigated. Notably. the& kavc be& no studies of cellular cytotoxicity in the presence of ~lasna. the physiologic milie;. We now hemonstrate the exist&e of a novel form of cellular cytotoxicity that is plasma and cell activation dependent. It is mediated both bv human peripheral blood hononuclear cells (PBM) and “by the transformed human monocytic cell line TIH-1. PDM and THP-I. when stimulated by LPS and in the presence of plasma, are cytotoxic to murine WEHI 164 cells in a 6 hr 51 Cr-release assay. Neither fresh nor heat-inactivated strum substitutes for ~plasma. We propose the designation of Plasma Dependent Cellular Cytotoxicity (PDCC) and suggest that this mu represent a novel effector mechanism in the host immune anb inflammatory response to transformed and other divergent cells.
169 1HE EFFECT ^_..
OF
SHORT-TEIU
TOPICAL
TRETINOIN
ON PHOTODAMAGED
bhlh
T. SHUKUWA' ST"UDEMAYER~
A.>,. KLIGMAN'
, X.
CHRISTENSEN'
and
T.
I Dept.
of Denn., Univ. af Pa. Schl. of Med., Phila., PA 'Biosearch Laboratories, Inc., Phila., PA Five males, 61, with severely p otodamaged skin received 0.057 tretinoin cream (Retin-A & , Ortho Pharmaceuticals) twice daily for one month to the skin of the dorsal forearm. The following procedures were done before and after treatment: (1) 3 mm punch biopsies for histochrmistrv, (2) sticky slides for degree of melanization of corneocytes, (3) detergent scrubs for corneacyte sizing and morpholagy, and (4) Silflo replicas for surface topography. The epidermal histologic abnormalities were largelv corrected by one month of treatment. Atypia and dysplasia were eliminated accompanied by acanthanosis, restoration of polarity and a thinned horny layer. Dermal changes were insignificant. Surface glyphic patterns reappeared. 'melanin granules within corneocytes were greatly decreased, associated with modest bleaching of
ages52 to
pigmented
spots.
171
172
A TRIAL TO CHOSE BATHING PRODUCTS IN PATIENTS WITH ATOPIC DERMATITIS T.
SUCAI,
Hospital,
Department
4-6-6,
of
Toyosaki,
Dermatology,
Kita.
Osaka
EFFECTIVE
Osaka
CIAGNOSIS OF LEPROSY BY PCR: HOW CAN PCR BE USED AS A SCREENING
Kaisei
531, Japan
I.
IN
DEVELOPING
S. KIM’and 4-l-l
COUNTRIES?
H. NAKAJIMAL, ‘National
Aoba-cho,
Higashimurayama
*Oepartment of Dermatology, Yokohama Fukuura, Yokohama 236, Japan.
A low
tolerance of the skin in patients with atopic to external stimuli, especially shampoos dermatitis However, some bathing and soaps, is well known. products wet-e proven to be effective in patients with atopic dermatitis, such as garlic BI extract (Shunrub@’ and herb shampoo containing hinokitiol (Eaudeleman The both products were found to be shampoo L a safe product with prophetic patch and photopatch showing a low value of skin irritation index. test, Both of them have a bacteriostatic action to prevent staphylococcal infection. it is necessary for products to have MOreOVer, Thus, a trial to an excellent moisturizing effect. chose bathing products effective in patients with atopic dermatitis was made, and it was found that a mixture of glycyrrhizic acid, hinokitiol and hinoki oil revealed moisturizing effect and suppressed a decrease of skin surface lipids.
TEST
Y. SUGITA’, Tama-Zenshoen,
)
City
Sanatorium Tokyo
University,
189,
and
3-9
Although some Investigators have already reported the detection of Mycobacterium leprae using the polymerase chain reaction (PCR), PCR is still not employed as a screening test in developing countries where such diagnostic systems are most needed. The purpose of this study was to highlight the problems involved in PCR detection of M. leprae and to suggest possible improvements in the practical use of PCR as a screening test even in developing countries. Our findings demonstrated the necessity for selectjon of highly specific primers, the most suitable samples for the screening test. It is concluded that a complete diagnostic system for leprosy using PCK can be established wlthout the need for radioisotopes, electrophores?s or membrane hybridization, which would enable a screening test to be done by PCR even in developing countries.
166
SUBCELLULAR PHARMACOKINETICSOF ‘OB1-DOPA ANALOGUE AS MELANOMA-SEEKING COMPOUND FOR BORON NEUTRON CAPTURE THERAPY M SHIONO’, C. HONDA’, M. ICHIHASHI’, T. SHIBATAs, Y. MISHIMA’~3 and B. KAGEDAL‘$ ‘Dept. of Derm., Kobe Uw. Sch. of Med., *Kobe Karser Hosprtal, 3Mrshima Inst. for Derm. Res Kobe Japan, “Dept. of Clrnrcal Chem The Univ of Lrnkopfng, Linkbping, Sweden
PLASMADEPENDENT CELLULAR CYTOTOXICITY: ANOVELTUMORCIDAL ACTIVITY OF THE MOfiOCYTIC CELL LIREAGE
10B1-paraboronophenylalanrne(loBt-BPA), dopa analogues synthesized and used capture
therapy(NCT).
has
a
one of the boronated by
us
selective
for thermal
affinity for
neutron
mallgnant
We have already successfully treated human melanoma cases by NCT usrng this compound In this study. we investigate the conhnuous In viva mrcrodralysts assay of “‘B-dynamics wrthrn rntercellular flurd of melanoma and normal subcutaneous t&sue We also report ‘OB concentratron and distribution dynamrcs withrn melanoma cells In the light of subcellular fractron analysis of excused melanoma tissue In order to elucrdate the mechamsm of the selectrve affrnrty of ‘061.BPA for malrgnant melanoma melanoma.
S .SHIRAHAMA’ , D.P.DIALYNAS2, T.S. EDGINGTON 'Dept. of Dermatol. Hamamatsu Univ. Sch. of Med., 3600 Ilanda-cho, Hamamatsu 431-31. Japan. 2Research Inst. of Scripps Clinic, L’SA. Cellular cytotoxicity mediated by cells of monocytes (no) lineaee. particulalv that with tumorcidal orooertics. has been kxtekively inkstigated. Notably. the& kavc be& no studies of cellular cytotoxicity in the presence of ~lasna. the physiologic milie;. We now hemonstrate the exist&e of a novel form of cellular cytotoxicity that is plasma and cell activation dependent. It is mediated both bv human peripheral blood hononuclear cells (PBM) and “by the transformed human monocytic cell line TIH-1. PDM and THP-I. when stimulated by LPS and in the presence of plasma, are cytotoxic to murine WEHI 164 cells in a 6 hr 51 Cr-release assay. Neither fresh nor heat-inactivated strum substitutes for ~plasma. We propose the designation of Plasma Dependent Cellular Cytotoxicity (PDCC) and suggest that this mu represent a novel effector mechanism in the host immune anb inflammatory response to transformed and other divergent cells.
169 1HE EFFECT ^_..
OF
SHORT-TEIU
TOPICAL
TRETINOIN
ON PHOTODAMAGED
bhlh
T. SHUKUWA' ST"UDEMAYER~
A.>,. KLIGMAN'
, X.
CHRISTENSEN'
and
T.
I Dept.
of Denn., Univ. af Pa. Schl. of Med., Phila., PA 'Biosearch Laboratories, Inc., Phila., PA Five males, 61, with severely p otodamaged skin received 0.057 tretinoin cream (Retin-A & , Ortho Pharmaceuticals) twice daily for one month to the skin of the dorsal forearm. The following procedures were done before and after treatment: (1) 3 mm punch biopsies for histochrmistrv, (2) sticky slides for degree of melanization of corneocytes, (3) detergent scrubs for corneacyte sizing and morpholagy, and (4) Silflo replicas for surface topography. The epidermal histologic abnormalities were largelv corrected by one month of treatment. Atypia and dysplasia were eliminated accompanied by acanthanosis, restoration of polarity and a thinned horny layer. Dermal changes were insignificant. Surface glyphic patterns reappeared. 'melanin granules within corneocytes were greatly decreased, associated with modest bleaching of
ages52 to
pigmented
spots.
171
172
A TRIAL TO CHOSE BATHING PRODUCTS IN PATIENTS WITH ATOPIC DERMATITIS T.
SUCAI,
Hospital,
Department
4-6-6,
of
Toyosaki,
Dermatology,
Kita.
Osaka
EFFECTIVE
Osaka
CIAGNOSIS OF LEPROSY BY PCR: HOW CAN PCR BE USED AS A SCREENING
Kaisei
531, Japan
I.
IN
DEVELOPING
S. KIM’and 4-l-l
COUNTRIES?
H. NAKAJIMAL, ‘National
Aoba-cho,
Higashimurayama
*Oepartment of Dermatology, Yokohama Fukuura, Yokohama 236, Japan.
A low
tolerance of the skin in patients with atopic to external stimuli, especially shampoos dermatitis However, some bathing and soaps, is well known. products wet-e proven to be effective in patients with atopic dermatitis, such as garlic BI extract (Shunrub@’ and herb shampoo containing hinokitiol (Eaudeleman The both products were found to be shampoo L a safe product with prophetic patch and photopatch showing a low value of skin irritation index. test, Both of them have a bacteriostatic action to prevent staphylococcal infection. it is necessary for products to have MOreOVer, Thus, a trial to an excellent moisturizing effect. chose bathing products effective in patients with atopic dermatitis was made, and it was found that a mixture of glycyrrhizic acid, hinokitiol and hinoki oil revealed moisturizing effect and suppressed a decrease of skin surface lipids.
TEST
Y. SUGITA’, Tama-Zenshoen,
)
City
Sanatorium Tokyo
University,
189,
and
3-9
Although some Investigators have already reported the detection of Mycobacterium leprae using the polymerase chain reaction (PCR), PCR is still not employed as a screening test in developing countries where such diagnostic systems are most needed. The purpose of this study was to highlight the problems involved in PCR detection of M. leprae and to suggest possible improvements in the practical use of PCR as a screening test even in developing countries. Our findings demonstrated the necessity for selectjon of highly specific primers, the most suitable samples for the screening test. It is concluded that a complete diagnostic system for leprosy using PCK can be established wlthout the need for radioisotopes, electrophores?s or membrane hybridization, which would enable a screening test to be done by PCR even in developing countries.