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The effect of swimming exercise on lipid peroxidation in rats
THE EFFECT OF SWIMMING EXERCISE ON LIPID PEROXIDATION IN RATS A. Coskun*, A. Golgeli’, C. Gzesm? and P. Dogan* Department of Biochemistry* and Physiolo&, Universrty of Erciyes, Faculty of Medicine, Kayseri. Turke_y
EFFECTS OF VITAMIN E AND ESTROGEN ON POST EXERCISE BLOOD ANTIOXIDANT STATUS AND MUSCULAR PERFORMANCE IN SEDENTARY WOMEN H. Giir, B. Akova, E. Stirmen-Giir, E. Gzttirk Department of Sports Medicine and Biochemistry, Uludag University, Bursa, Turkey
Oxygen free radicals are highly reactive species that can cause a wide spectrum of cell damage including lipid peroxidation, enzymes inactivation and DNA damage. Strenuous physical exercise has been shown to impose an oxidative stress on the body due to oxygen free radical generation including an increase in lipid peroxidation. These facts pertain especially to acute or unaccustomed exercise. The purpose of the present study was to examine the effect of the acute swimming exercise on lipid peroxidation. Studies were performed on 20 adult male Swiss albino rats. Animals were divided into 2 groups, 10 rats control group and 9 similar rats exercise group. After exercise blood was taken to measure erythrocyte MDA and haemoglobin levels. Results were expressed as nmol MDAigr Hb. MDA levels from exercise group were not significantly different from control group, with values of 27.4k9.2 and 255.t7.3 nmol MDA/gr Hb respectively (P>O.O5). We concluded that 1 hour swimming exercise doesn’t increase lipid peroxidation in erythrocyte.
To investigate the effects of vitamin E (Vit E) and estrogen on post-exercise (post-ex) blood antioxidant status and muscular performance, 18 healthy sedentary women, aged 19-35 years, who had regular menstrual cycle, volunteered for this study. The volunteers were randomly divided into two groups as Vit E group (n=lO) and placebo group (n=8). The subjects in V’it E and placebo groups took 300 mg a-tocopherol and placebo, daily, respectively. After 42 days of supplementation blood Vit E level was increased (p < 0.001) merely in Vit E group. Subjects performed three bouts of an exhaustive isokinetic exercise at the menstrual CMP) and preovulatory phases (PO). Pre- exercise (pre-ex) estrogen levels in PO of both groups were greater (p < 0.001) than in MP of both groups and pre-ex blood Vit E levels were also greater (p < 0.001) in Vit E group compared to placebo group in both phases. Concentric and eccentric total work, duration and number of repetitions until fatigue were not different between the groups and phases. While post-ex SOD activity decreased significantly in PO and MP of both groups, decrement in GPx activity was significant in only MP of both groups. In addition, the significances in SOD were more remarkahle in MP of both groups compared to PO. Therefore. we concluded that estrogen could influence post-ex blood antioxidant status but not muscular performance while VI: E had no influence on these subjects alone and/or together with estrogen. However, these results can be specified for sedentary subjects and the present exercise model.
ENDURANCE TRAINING MAY DECREASE OXIDATIVE STRESS IN STREPTOZOTOCIN-INDUCED DIABETIC BAT Mustafa GUI, David E. Laaksonen, Mustafa Atalay,
OXYGEN KINETIC CHANGES IN WITH SYSTEMIC COLLAGENOSES
Savita Khanna, Lena Vider, Sashwati Chandan K. Sen,
Dobroslav
Hajek,
Zdenek
Fojtik,
Andrea
PATIENTS
krivanova,
Hana
Kubesova
Roy, Osmo Hannmen,
Masaryk Republic
Department of Physiology. University of Kuopio, FINLAND
University
Hospital
Brno-Bohunice,
Czech
We tested 146 probands both sexes aged 45*23 years and 9 patients with systemic collagenoses (8 women, 1 man, age 52*11, duration of disease lM4 years) whith systemic lupus erythematosus, Sharp syndrome, and sclerodermia to contribute in the oxygen kinetic changes in collagenoses. The method is based on the testing of transcutaneous oxygen pressure increase during the inhalation of hyperoxic mixture and model calculation. Not only the increased right to left shunt (15&5 per cent), decreased oxygen lung diffusion (68*27 of normal value) but also the decreased tissue oxygen diffision coefficient to about l/100 compared to healthy were proved in 6 patients, 3 patients revealed oxygen tissue diffusion coefficient fairly not different from the other 146 probands but exhibited oxygen lung diffusion changes. The increased intracellular oxygen concentration could play an important role in the pathogenesis of collagenoses. The correlation between lungs involvement and decreased oxygen tissue diffusion was not proved on collagenoses patients.
Drabetes mellitus is associated with increased oxidative stress, which is proposed to have a role in micro- and macrovascular comphcations. The aim of our study was to assess whether endurance training (ET) decreases oxidative stress m diabetes. Streptozotocin-induced diabetic rats (n=34) were divided into trained and untrained groups. These groups were further dlvrded into rats killed at rest (UR and TR) and immediately after exhaustive exercise (UE, TE). ET consisted of treadmill running up to 2.1 km/h, 1.5 hid, 5 days a week for 8 weeks. For acute exhaustive exercise (EE), graded treadmill running starting at 1.8 km/h continued until exhaustion. ET decreased thiobarbthtrtc acid reactive substances (TBARS) levels m kidney and vastus lateralis muscle at rest. However, ET did not affect TBARS levels in red gastrocnemms muscle, heart and liver. ET did not affect total glutathione (TGSH) levels in blood and tissues at rest. While ET increased glutathione peroxrdase acttvity in red gastrocnemius muscle, it decreased glutathione peroxidase activity in heart at rest. EE did not affect ttssue TBARS levels. EE decreased TGSH levels in kidney in endurance trained rats only. Glutathione peroxidase activrty increased in red gastrocnemius muscle, but decreased m ktdney after EE in endurance trained rats. Glutathione disulfide reductase and glutathione S-transferase activities were not affected by either ET or EE. Our results suggest that endurance training may decrease oxidative stress m at least the vastus lateralis muscle and kidney in streptozotocin-induced dtabetrc rats.
115
EFFECTS OF VITAMIN E AND ESTROGEN ON POST EXERCISE BLOOD ANTIOXIDANT STATUS AND MUSCULAR PERFORMANCE IN SEDENTARY WOMEN H. Giir, B. Akova, E. Stirmen-Giir, E. Gzttirk Department of Sports Medicine and Biochemistry, Uludag University, Bursa, Turkey
Oxygen free radicals are highly reactive species that can cause a wide spectrum of cell damage including lipid peroxidation, enzymes inactivation and DNA damage. Strenuous physical exercise has been shown to impose an oxidative stress on the body due to oxygen free radical generation including an increase in lipid peroxidation. These facts pertain especially to acute or unaccustomed exercise. The purpose of the present study was to examine the effect of the acute swimming exercise on lipid peroxidation. Studies were performed on 20 adult male Swiss albino rats. Animals were divided into 2 groups, 10 rats control group and 9 similar rats exercise group. After exercise blood was taken to measure erythrocyte MDA and haemoglobin levels. Results were expressed as nmol MDAigr Hb. MDA levels from exercise group were not significantly different from control group, with values of 27.4k9.2 and 255.t7.3 nmol MDA/gr Hb respectively (P>O.O5). We concluded that 1 hour swimming exercise doesn’t increase lipid peroxidation in erythrocyte.
To investigate the effects of vitamin E (Vit E) and estrogen on post-exercise (post-ex) blood antioxidant status and muscular performance, 18 healthy sedentary women, aged 19-35 years, who had regular menstrual cycle, volunteered for this study. The volunteers were randomly divided into two groups as Vit E group (n=lO) and placebo group (n=8). The subjects in V’it E and placebo groups took 300 mg a-tocopherol and placebo, daily, respectively. After 42 days of supplementation blood Vit E level was increased (p < 0.001) merely in Vit E group. Subjects performed three bouts of an exhaustive isokinetic exercise at the menstrual CMP) and preovulatory phases (PO). Pre- exercise (pre-ex) estrogen levels in PO of both groups were greater (p < 0.001) than in MP of both groups and pre-ex blood Vit E levels were also greater (p < 0.001) in Vit E group compared to placebo group in both phases. Concentric and eccentric total work, duration and number of repetitions until fatigue were not different between the groups and phases. While post-ex SOD activity decreased significantly in PO and MP of both groups, decrement in GPx activity was significant in only MP of both groups. In addition, the significances in SOD were more remarkahle in MP of both groups compared to PO. Therefore. we concluded that estrogen could influence post-ex blood antioxidant status but not muscular performance while VI: E had no influence on these subjects alone and/or together with estrogen. However, these results can be specified for sedentary subjects and the present exercise model.
ENDURANCE TRAINING MAY DECREASE OXIDATIVE STRESS IN STREPTOZOTOCIN-INDUCED DIABETIC BAT Mustafa GUI, David E. Laaksonen, Mustafa Atalay,
OXYGEN KINETIC CHANGES IN WITH SYSTEMIC COLLAGENOSES
Savita Khanna, Lena Vider, Sashwati Chandan K. Sen,
Dobroslav
Hajek,
Zdenek
Fojtik,
Andrea
PATIENTS
krivanova,
Hana
Kubesova
Roy, Osmo Hannmen,
Masaryk Republic
Department of Physiology. University of Kuopio, FINLAND
University
Hospital
Brno-Bohunice,
Czech
We tested 146 probands both sexes aged 45*23 years and 9 patients with systemic collagenoses (8 women, 1 man, age 52*11, duration of disease lM4 years) whith systemic lupus erythematosus, Sharp syndrome, and sclerodermia to contribute in the oxygen kinetic changes in collagenoses. The method is based on the testing of transcutaneous oxygen pressure increase during the inhalation of hyperoxic mixture and model calculation. Not only the increased right to left shunt (15&5 per cent), decreased oxygen lung diffusion (68*27 of normal value) but also the decreased tissue oxygen diffision coefficient to about l/100 compared to healthy were proved in 6 patients, 3 patients revealed oxygen tissue diffusion coefficient fairly not different from the other 146 probands but exhibited oxygen lung diffusion changes. The increased intracellular oxygen concentration could play an important role in the pathogenesis of collagenoses. The correlation between lungs involvement and decreased oxygen tissue diffusion was not proved on collagenoses patients.
Drabetes mellitus is associated with increased oxidative stress, which is proposed to have a role in micro- and macrovascular comphcations. The aim of our study was to assess whether endurance training (ET) decreases oxidative stress m diabetes. Streptozotocin-induced diabetic rats (n=34) were divided into trained and untrained groups. These groups were further dlvrded into rats killed at rest (UR and TR) and immediately after exhaustive exercise (UE, TE). ET consisted of treadmill running up to 2.1 km/h, 1.5 hid, 5 days a week for 8 weeks. For acute exhaustive exercise (EE), graded treadmill running starting at 1.8 km/h continued until exhaustion. ET decreased thiobarbthtrtc acid reactive substances (TBARS) levels m kidney and vastus lateralis muscle at rest. However, ET did not affect TBARS levels in red gastrocnemms muscle, heart and liver. ET did not affect total glutathione (TGSH) levels in blood and tissues at rest. While ET increased glutathione peroxrdase acttvity in red gastrocnemius muscle, it decreased glutathione peroxidase activity in heart at rest. EE did not affect ttssue TBARS levels. EE decreased TGSH levels in kidney in endurance trained rats only. Glutathione peroxidase activrty increased in red gastrocnemius muscle, but decreased m ktdney after EE in endurance trained rats. Glutathione disulfide reductase and glutathione S-transferase activities were not affected by either ET or EE. Our results suggest that endurance training may decrease oxidative stress m at least the vastus lateralis muscle and kidney in streptozotocin-induced dtabetrc rats.
115