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The Effect of Trigeminal Neurosensory Deficit on Quality of Life
Poster Session The incidence of patients and sinus membrane thickening according to our grading criteria was grade 2 = 36.8% patients/24.3% sinuses; grade 3 = 6.0% patients/ 3.7% sinuses, and grade 4 = 3.6% patients/2.2% sinuses. Unilateral sinus disease was predominant. Of those sinuses with thickening, 80.6% were grade 2, 12.2% were grade 3, and 7.2% were grade 4. Of the 469 sinuses with membrane thickening, 210 were adjacent to unhealthy teeth , 233 were adjacent to healthy teeth, and 26 were in edentulous maxillas. Of the 210 unhealthy teeth , 30 had post-extraction cone beams available for evaluation. The grade 2 sinus membrane thickening showed a 75% resolution to grade 1 after adjacent tooth removal. The grade 3 sinuses resolved 25% to grade 1 and grade 2, with 50% remaining at grade 3. There were 2 grade 4 sinuses with followup scans with 1 resolving to grade 2 and the other remaining at grade 4. Conclusions: Sinus membrane thickening is present in 46.7% of patients coming to an Oral and Maxillofacial Surgery practice. The incidence of sinus membrane thickening was almost equal in association with unhealthy or healthy teeth. The removal of the unhealthy teeth decreased but did not completely resolve sinus membrane thickening. References: 1. Nair UP, Nair MK. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 110:e53-57, 2010. 2. Pokorny A, Tataryn R. Int Forum Allergy Rhinol. 3:973-979, 2013.
POSTER 44 The Effect of Biomechanical Stimulation on Osteoblast Differentiation of Human Jaw Periosteum Derived Stem Cells J. M. Lee: School of Dentistry, Pusan National University, Pusan National University Dental Hospital, White Medical Group, U. K. Kim Purpose: This study was to investigate the effect of biomechanical stimulation on osteoblast differentiation of human periosteal derived stem cell using the newly developed bioreactor. Materials and Methods: Human peristeal derived stem cells were harvested from the mandible during the extraction of an impacted third molar. Using the new bioreactor, 4 % cyclic equibiaxial tension force (0.5Hz) was applied for 2 and 8 hours on the stem cells, and cultured for 3, 7, and 14 days on the osteogenic medium. Biochemical changes of the osteoblasts after the biomechanical stimulation were investigated. No treatment group was referred to as control group. Results: ALP activity and ALP mRNA expression level were higher in the strain group than those of the control group. The osteocalcin and osteonectin mRNA expressions were higher in the strain group to the control group e-76
on days 7 and 14. The VEGF mRNA expression was higher in the strain group in comparison to the control group. Concentration of Alizarin Red S corresponding to calcium content was higher in the strain group than the control group. Conclusion: The study suggests that cyclic tension force could influence the osteoblast differentiation of periosteal derived stem cells under optimal stimulation condition and the force could be applicable for tissue engineering.
POSTER 45 The Effect of Trigeminal Neurosensory Deficit on Quality of Life M. Cakir: Gazi University Faculty of Dentistry, E. Peker, F. Ogutlu, H. Yazar, I. Karaca Dental treatments may cause damages on sensory nerves of the jaws which are branches of the trigeminal nerve. Patient’s quality of life can be affected by nerve injuries that may cause pain. There are a number of surveys to assess quality of life in dental procedures. The purpose of this study was to investigate the effect of temporary or persistent neurosensory disturbance of the lingual nerve (LN) or inferior alveolar nerve (IAN) on quality of life. Oral Health Impact Profile (OHIP-14) was used to determine the effects of temporary or persistent neurosensory disturbance of LN or IAN on quality of life. The study design was a case-control study. The patients divided into two groups, each containing 50% of the patients; (n=100 per group). The patients with the neurosensory deficit of the LN and/or the IAN, developed as a consequence of dental or oral and maxillofacial surgery procedures that confirmed by subjective and objective neurosensory tests formed the Study Group. The patients who had dental or oral and maxillofacial surgery procedures in the same unit and did not present with neurosensory deficit were matched with the Study Group according to their gender and age. They were presented as the Control Group in the study. All patients filled in a Turkish language translated version of the 14-item Oral Health Impact Profile (OHIP- 14). Oral HealthRelated Quality of Life (OHRQoL) was assessed using OHIP-14 across seven topics (two questions per topic) by asking patients to rate the frequency of occurrence of a particular problem. In this questionnaire the topics are: functional limitation, physical pain, psychological discomfort, physical disability, psychological disability, social disability, and handicap. . The higher the total score is the higher level of negative impact on OHRQoL is. The results were evaluated with SPSS 21.0 software package. Statistical analyses were performed by use of single sample Kolmogrov-Smirnov test for normality and the Levene test for homogeneity and the differences of the groups were evaluated with Mann-Whitney U and Kruskal Wallis tests. AAOMS 2014
Poster Session This study included 200 patients (122 female, 78 male), aged between 18 and 75, with a mean of 37 years. The OHIP-14 scores were higher in this patients with neurosensory deficits compared with the control group. There were significantly differences between study and control groups in functional limitation (p=0,001), psychological discomfort (p=0,000), psychological disability (p=0,000), and handicap (p=0,000) subgroups. It was concluded that the patients with the neurosensory deficit of the LN and/or the IAN have poorer OHRQoL than those without neurosensory deficits. References: 1 Slade GD. Derivation and validation of a short-form oral health impact profile. Community Dent Oral Epidemiol 1997:25, 284-290. 2 Lam NP, Donoff RB, Kaban LB, Dodson TB. Patient satisfaction after trigeminal nerve repair. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003 May;95(5):538-43.
POSTER 46 An experimental study on the effects of platelet rich plasma on the woundhealing of tooth extraction-related bone defects K. Niimi: Division of Reconstructive Surgery for Oral and Maxillofacial Region, Course for Oral Life science, Niigata University Graduate School of Medical and Dental Sciences, M. Yoshizawa, T. Koyama, A. Funayama, T. Mikami, T. Kobayashi Platelet-rich plasma (PRP) is an autologous preparation of platelets in concentrated plasma. Although PRP has been used as a therapeutic tool to promote wound healing, the mechanisms underlying the wound healing induced by PRP remain unclear. The objective of this study was to evaluate the effectiveness of PRP on the healing of tooth extraction-related sockets. After being anesthetized using sevoflurane inhalation and the intraperitoneal administration of chloral hydrate, all three upper molars of 10-week-old male Fischer rats were extracted, and the bone between the molars was removed with a round burr to create bone defects approximately 113 mm in size. Blood was drawn from the hearts of other rats, and PRP was prepared. The PRP preparation was obtained from age-matched Fisher rats. Heart puncture with an 18-G needle and exsanguination were performed, and blood was collected into tubes. The tubes were centrifuged at 450x G for 10 minutes. After the first spin, the serum, buffy coat and red cells located 2 mm under the buffy coat were aspirated, collected into another tube and centrifuged at 850x G for 15 minutes. The PRP was collected at the bottom of the second tube and activated with bovine thrombin and calcium chloride. The PRP was applied to the bone defects of the experimental group while nothing was applied to the bone defects of the control AAOMS 2014
group. The rats were sacrificed on days 1, 3, 5 and 7. The specimens were fixed with 20% buffered formalin and decalcified with EDTA. Serial sections measuring 7 mm in diameter were obtained and stained with hematoxylin and eosin. The sections were studied immunohistochemically for type IV collagen in order to visualize the basement membrane, and specific staining was also performed to visualize collagen fibers. The results showed that inflammatory cells and a fibrin network were observed under the PRP layer in the bone defects in the experimental group, whereas inflammatory cells and a fibrin network were rarely seen in the control group on day 1. On day 3, only inflammatory cells and a fibrin network were observed in the bone defects of the control group, while conglomerates of inflammatory cells, a fibrin network and granulation tissue, which is abundant in blood capillaries, were seen under the PRP layer in the experimental group. On day 5, a thin layer of granulation tissue was observed in limited areas of the bone defects in the control group, while thick granulation tissue was seen in wide areas of the bone defects in the experimental group. On day 7, granulation tissues were seen in bone defect of both control group and experimental group, but concavity was seen in the control group and a thicker granulation layer was observed in the experimental group. At the point of epithelialization, earlier epithelialization was seen on thicker granulation tissues on days 5 and 7 in the experimental group compared to the control group. In conclusion, in this study, PRP treatment induced a rich fibrin network and early inflammatory reactions in the experimental group, which may have led to the development of thick, vessel-rich granulation tissue with early epithelialization. The growth factors secreted from the granulation tissue may therefore have led to the observed early wound healing in the experimental group. Consequently, the application of PRP may stimulate wound healing in tooth extraction-related bone defects. References: 1. Efficacy of Platelet-Rich Plasma on Wound Healing in Rabbits. H W. Lee et al.; J Periodontol 2008; 79: 691-696. 2. Platelet-rich plasma (PRP) in dental and oral surgery: from the wound healing to bone regeneration. Albanese et al; Immunity & Ageing 2013 10: 23.
POSTER 47 Comparative study on surgical and conservative management of Bisphosphonate-related osteonecrosis of the jaw(BRONJ) J. K. Lee: Ajou University School of Medicine, Y. H. Kim, H. K. Lee Purpose: This study evaluated the prognosis of conservative and surgical treatment of BRONJ e-77
POSTER 44 The Effect of Biomechanical Stimulation on Osteoblast Differentiation of Human Jaw Periosteum Derived Stem Cells J. M. Lee: School of Dentistry, Pusan National University, Pusan National University Dental Hospital, White Medical Group, U. K. Kim Purpose: This study was to investigate the effect of biomechanical stimulation on osteoblast differentiation of human periosteal derived stem cell using the newly developed bioreactor. Materials and Methods: Human peristeal derived stem cells were harvested from the mandible during the extraction of an impacted third molar. Using the new bioreactor, 4 % cyclic equibiaxial tension force (0.5Hz) was applied for 2 and 8 hours on the stem cells, and cultured for 3, 7, and 14 days on the osteogenic medium. Biochemical changes of the osteoblasts after the biomechanical stimulation were investigated. No treatment group was referred to as control group. Results: ALP activity and ALP mRNA expression level were higher in the strain group than those of the control group. The osteocalcin and osteonectin mRNA expressions were higher in the strain group to the control group e-76
on days 7 and 14. The VEGF mRNA expression was higher in the strain group in comparison to the control group. Concentration of Alizarin Red S corresponding to calcium content was higher in the strain group than the control group. Conclusion: The study suggests that cyclic tension force could influence the osteoblast differentiation of periosteal derived stem cells under optimal stimulation condition and the force could be applicable for tissue engineering.
POSTER 45 The Effect of Trigeminal Neurosensory Deficit on Quality of Life M. Cakir: Gazi University Faculty of Dentistry, E. Peker, F. Ogutlu, H. Yazar, I. Karaca Dental treatments may cause damages on sensory nerves of the jaws which are branches of the trigeminal nerve. Patient’s quality of life can be affected by nerve injuries that may cause pain. There are a number of surveys to assess quality of life in dental procedures. The purpose of this study was to investigate the effect of temporary or persistent neurosensory disturbance of the lingual nerve (LN) or inferior alveolar nerve (IAN) on quality of life. Oral Health Impact Profile (OHIP-14) was used to determine the effects of temporary or persistent neurosensory disturbance of LN or IAN on quality of life. The study design was a case-control study. The patients divided into two groups, each containing 50% of the patients; (n=100 per group). The patients with the neurosensory deficit of the LN and/or the IAN, developed as a consequence of dental or oral and maxillofacial surgery procedures that confirmed by subjective and objective neurosensory tests formed the Study Group. The patients who had dental or oral and maxillofacial surgery procedures in the same unit and did not present with neurosensory deficit were matched with the Study Group according to their gender and age. They were presented as the Control Group in the study. All patients filled in a Turkish language translated version of the 14-item Oral Health Impact Profile (OHIP- 14). Oral HealthRelated Quality of Life (OHRQoL) was assessed using OHIP-14 across seven topics (two questions per topic) by asking patients to rate the frequency of occurrence of a particular problem. In this questionnaire the topics are: functional limitation, physical pain, psychological discomfort, physical disability, psychological disability, social disability, and handicap. . The higher the total score is the higher level of negative impact on OHRQoL is. The results were evaluated with SPSS 21.0 software package. Statistical analyses were performed by use of single sample Kolmogrov-Smirnov test for normality and the Levene test for homogeneity and the differences of the groups were evaluated with Mann-Whitney U and Kruskal Wallis tests. AAOMS 2014
Poster Session This study included 200 patients (122 female, 78 male), aged between 18 and 75, with a mean of 37 years. The OHIP-14 scores were higher in this patients with neurosensory deficits compared with the control group. There were significantly differences between study and control groups in functional limitation (p=0,001), psychological discomfort (p=0,000), psychological disability (p=0,000), and handicap (p=0,000) subgroups. It was concluded that the patients with the neurosensory deficit of the LN and/or the IAN have poorer OHRQoL than those without neurosensory deficits. References: 1 Slade GD. Derivation and validation of a short-form oral health impact profile. Community Dent Oral Epidemiol 1997:25, 284-290. 2 Lam NP, Donoff RB, Kaban LB, Dodson TB. Patient satisfaction after trigeminal nerve repair. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2003 May;95(5):538-43.
POSTER 46 An experimental study on the effects of platelet rich plasma on the woundhealing of tooth extraction-related bone defects K. Niimi: Division of Reconstructive Surgery for Oral and Maxillofacial Region, Course for Oral Life science, Niigata University Graduate School of Medical and Dental Sciences, M. Yoshizawa, T. Koyama, A. Funayama, T. Mikami, T. Kobayashi Platelet-rich plasma (PRP) is an autologous preparation of platelets in concentrated plasma. Although PRP has been used as a therapeutic tool to promote wound healing, the mechanisms underlying the wound healing induced by PRP remain unclear. The objective of this study was to evaluate the effectiveness of PRP on the healing of tooth extraction-related sockets. After being anesthetized using sevoflurane inhalation and the intraperitoneal administration of chloral hydrate, all three upper molars of 10-week-old male Fischer rats were extracted, and the bone between the molars was removed with a round burr to create bone defects approximately 113 mm in size. Blood was drawn from the hearts of other rats, and PRP was prepared. The PRP preparation was obtained from age-matched Fisher rats. Heart puncture with an 18-G needle and exsanguination were performed, and blood was collected into tubes. The tubes were centrifuged at 450x G for 10 minutes. After the first spin, the serum, buffy coat and red cells located 2 mm under the buffy coat were aspirated, collected into another tube and centrifuged at 850x G for 15 minutes. The PRP was collected at the bottom of the second tube and activated with bovine thrombin and calcium chloride. The PRP was applied to the bone defects of the experimental group while nothing was applied to the bone defects of the control AAOMS 2014
group. The rats were sacrificed on days 1, 3, 5 and 7. The specimens were fixed with 20% buffered formalin and decalcified with EDTA. Serial sections measuring 7 mm in diameter were obtained and stained with hematoxylin and eosin. The sections were studied immunohistochemically for type IV collagen in order to visualize the basement membrane, and specific staining was also performed to visualize collagen fibers. The results showed that inflammatory cells and a fibrin network were observed under the PRP layer in the bone defects in the experimental group, whereas inflammatory cells and a fibrin network were rarely seen in the control group on day 1. On day 3, only inflammatory cells and a fibrin network were observed in the bone defects of the control group, while conglomerates of inflammatory cells, a fibrin network and granulation tissue, which is abundant in blood capillaries, were seen under the PRP layer in the experimental group. On day 5, a thin layer of granulation tissue was observed in limited areas of the bone defects in the control group, while thick granulation tissue was seen in wide areas of the bone defects in the experimental group. On day 7, granulation tissues were seen in bone defect of both control group and experimental group, but concavity was seen in the control group and a thicker granulation layer was observed in the experimental group. At the point of epithelialization, earlier epithelialization was seen on thicker granulation tissues on days 5 and 7 in the experimental group compared to the control group. In conclusion, in this study, PRP treatment induced a rich fibrin network and early inflammatory reactions in the experimental group, which may have led to the development of thick, vessel-rich granulation tissue with early epithelialization. The growth factors secreted from the granulation tissue may therefore have led to the observed early wound healing in the experimental group. Consequently, the application of PRP may stimulate wound healing in tooth extraction-related bone defects. References: 1. Efficacy of Platelet-Rich Plasma on Wound Healing in Rabbits. H W. Lee et al.; J Periodontol 2008; 79: 691-696. 2. Platelet-rich plasma (PRP) in dental and oral surgery: from the wound healing to bone regeneration. Albanese et al; Immunity & Ageing 2013 10: 23.
POSTER 47 Comparative study on surgical and conservative management of Bisphosphonate-related osteonecrosis of the jaw(BRONJ) J. K. Lee: Ajou University School of Medicine, Y. H. Kim, H. K. Lee Purpose: This study evaluated the prognosis of conservative and surgical treatment of BRONJ e-77