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The effects of different concentration of opium on the secretion of TGF-β in Jurkat cells
S134
Abstracts
Molondialdehyde (MDA) as a marker for lipid peroxdation were determined. Results: Significant increases were found in the plasma MDA level. Activities of glutathione peroxidase and glutathione reductase in plasma were decreased in OCP users compared to the control group. Conclusion: These data suggest that low-dose oral contraceptives, by enhancing the oxidative stress and lipid peroxidation, may represent a potential cardiovascular risk factor. Keywords: Stress oxidative, Lipid peroxidation, Oral contraceptives doi:10.1016/j.clinbiochem.2011.08.319
Poster – [A-10-175-2] The comparison of the level of the heat shock protein 70 between neonate with perinatal asphyxia and healthy neonate Hasan Boskabadi, Masoud Omidian, Shima Tavallai, Shabnam Mohammadi, Mostafa Parizadeh, Majid Ghayour-Mobarhan a Mashhad University of Medical Sciences, Mashhad, Iran b Biochemical and Nutritional Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran E-mail address: [email protected] (M. Ghayour-Mobarhan) Introduction: Perinatal asphyxia is an important cause of death and permanent neurological and developmental complications. Early and accurate diagnosis not only prognosis but also in determining the proper treatment would be helpful, according to animal studies conducted in conditions of cellular stress, including heat and ischemia, a protein called HSP70, which appeared to be good and can be a useful marker of cellular stress and might be able to determine the neonatal asphyxia in faster, more accurate diagnosis known. Method: In this observational study, the level of HSP 70 was compared between neonates suffering from perinatal asphyxia (n= 50) and normal neonates (n= 51), whom were recruited from Ghaem hospital, Mashhad, Iran. Term neonates without major anomalies who had asphyxia during delivery were enrolled in the first six hours after delivery, and control group were healthy term neonates without problems and the delivery process during the first week of life and their cord blood during labor to measure HSP 70 by using in-house ELISA. Results: The median values of serum anti HSP70 titers were significantly higher in asphyxiated neonates compared with non asphyxiated neonates (0.36 [0.04–1.14] vs. 0.24 [0.01–0.63]. At cut off point= 0.31 AU, sensitivity was 58% and specificity was 76%. Conclusion: A significant difference between control and patient groups in the level of HSP70 was observed in this study. Also, HSP70 could be a good marker for the early diagnosis of prenatal hypoxia. Keywords: Neonatal asphyxia, Hypoxic–ischemic encephalopathy, Heat shock protein 70 doi:10.1016/j.clinbiochem.2011.08.320
Poster – [A-10-194-1] Association between RAGE (Receptor for Advanced Glycation End products) and lipid peroxidation in sperm of diabetic men Jamshid Karimia, Taghi Goodarzi Mohammada, Heidar Tavilania, Iraj Khodadadia, Iraj Amirib, Hossein Pirya a Department of Biochemistry, Hamadan University of Medical Science, Iran b Department of Anatomy, Hamadan University of Medical Science, Iran E-mail address: [email protected] (J. Karimi)
Introduction: Majority of diabetic male patients have disturbances in their reproductive systems. However, the mechanisms underlying these disturbances are largely unknown. Since receptor for advanced glycation end products (RAGE) have a key role in oxidative stress and cell damage in diabetic complications, we hypothesize that RAGE may be involved in sperm lipid peroxidation. Materials and methods: RAGE levels in sperm of 32 diabetic and 35 non-diabetic men were determined using ELISA and western blot analysis. Contents of lipid peroxidation in sperm were determined by thiobarbituric acid reaction. Results: RAGE protein levels were found significantly higher in sperm of diabetic men than non-diabetic group (p < 0.001). Moreover, sperm lipid peroxidation was significantly higher in diabetic subjects than non-diabetic men. Sperm RAGE was directly correlated to sperm lipid proxidation in diabetic men (r = 0.69, p < 0.001). Conclusions: A highly positive correlation between RAGE levels and lipid peroxidation in sperm of diabetic men may be the elucidating central role of RAGE in disturbances in sexual function of diabetic men. Keywords: RAGE, Sperm, Lipid peroxidation, Diabetes doi:10.1016/j.clinbiochem.2011.08.321
Poster – [A-10-209-1] The effects of different concentration of opium on the secretion of TGF-β in Jurkat cells Gholamreza Asadikarama, Somayeh Eigderb, Mohammad Kazemi-arababadic, Mehdi Mahmoodib a Department of Biochemistry and Physiology Research Center, Kerman University Of Medical Sciences, Kerman, Iran b Dept of Biochemistry Faculty of Medicine, Rafsanjan University of Medical Sciences, Iran c Dept of Immunology Faculty of Medicine, Rafsanjan University of Medical Sciences, Iran E-mail addresses: [email protected] (G. Asadikaram), [email protected] (S. Eigder), [email protected] (M. Kazemi-arababadi), [email protected] (M. Mahmoodi) Introduction: Transforming growth factor-β (TGF-β) is an important cytokine that has a broad spectrum of biological functions and actions in a variety of cell types. This cytokine has inhibitory effect on the immune system and diminished production of this cytokine is associated with autoimmune disorders. The aim of this study was to evaluate the influence of different concentration of opium on the secretion of TGF-β in Jurkat cells. Material and methods: In the current study, different concentrations of opium (2.85 × 10− 1, 2.85 × 10− 3, 2.85 × 10− 5, 2.85 × 10− 7, 2.85 × 10− 9, 2.85 × 10− 11 g/ml) were added to 24-well plates containing 5 × 105 Jurkat cells. The concentration of TGF-β was measured after 6, 12, 24, 48, and 72 hours (h) by ELISA . P < 0.05 was considered as the statistically significant level. Results: Our results indicated that the mean level of TGF-β in Jurkat cells was significantly higher at: 6 h in concentrations of 2.85 × 10− 1 and 2.85 × 10− 5 g/ml, at 24 and 48 h in concentrations of 2.85 × 10− 9, 2.85 × 10 − 11 g/ml. However, in concentrations of 2.85 × 10− 3, 2.85 × 10− 5, and 2.85 × 10− 7 at 48 h and concentration of 2.85 × 10− 3, 2.85 × 10− 5 and 2.85 × 10− 7 at 72 h the mean amount of TGF-β was obviously lower compared with control. Conclusion: These results showed that opium impacts immune system and cell function through a change in the level of TGF-β in T lymphocyte and this effect depends on both dose and times. This effect may play a role in immune dysfunction in opium addicts.
Abstracts
Keywords: Opium, TGF-β, Jurkat cell doi:10.1016/j.clinbiochem.2011.08.322
Poster – [A-10-223-3] Comparison of prevalence beta lactamase enzyme in bacteria isolated of clinical sample Shilla Jalalpoora, Abousaidi Hamidb a Islamic Azad University, Shahreza Branch, Shahreza, Iran b University of Medical Science, Rafsanjan, Iran E-mail address: [email protected] (S. Jalalpoor) Introduction: Beta lactame antibiotics are very important in the cure of diseases. Beta lactamase is a virulence agent and causes resistance to these antibiotics. Spread of beta lactamase in pathogen bacteria can be a disorder to cure. The aim of this search is the comparison of prevalence of beta lactamase enzyme in isolated clinical sample Bacteria in Alzahra hospital. Materials and methods: The research was performed with laboratory method in the years 1384/1385, in Alzahra hospital in Isfahan. According to statistical formula 100 infection samples (blood, urine, skin) were randomly selected and bacteria were transmitted into the laboratory. Identification of bacteria was performed with microbiological methods, for example: staining, chemical test, use of differential and selective media. For determine beta lactamase production in bacteria, use acidimetric method. All the statistical analyses were carried out using SPSS version 14. Chi-square and fisher test were used for determination of significance of association. The p ≤ 0.05 was considered significant. Results: From 100 isolated pathogen bacteria 68/31% produced beta lactamase. Respectively, these were Staphylococcus spp. 83.33%, Enterobacteriaceae spp. 70.95% and Pseudomonas 18.8%. Conclusion: The reason for the prevalence of beta lactamase enzyme in isolated bacteria of nosocamial infection, which was very high, was due to increased resistanceto beta lactam antibiotics in pathogen bacteria. Keywords: Beta lactamase, Pathogen bacteria, Clinical sample, Beta lactam antibiotics
doi:10.1016/j.clinbiochem.2011.08.323
Poster – [A-10-228-1] Serum levels of Interleukin (IL)-10 in 11–17 years old girls with vitamin D deficiency and control group Zahra Jamalia, Gholamreza Asadikarama, Mohammad Kazemi-arababadib a Dept. Of Biochemistry and Physiology Research center, Kerman University of medical Sciences, Kerman, Iran b Dept. of Microbiology, Hematology and Immunology, Faculty of Medicine and Molecular-Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran E-mail addresses: [email protected] (Z. Jamali), [email protected] (G. Asadikaram), [email protected] (M. Kazemi-arababadi) Introduction: Vitamin D not only is essential for bone health and preventing of many chronic diseases but also it recognized as an important modulator of the immune system. The aim of this study was to examine the serum levels of the IL-10, as a regulatory cytokine that prevent elevated production of inflammatory cytokines, in vitamin D deficient adolescent girls compared to control group.
S135
Materials and methods: Serum samples of 100 girls with vitamin D deficiency aged 11–17 years, and 100 healthy girls were selected and serum levels of IL-10 were measured by ELISA. Results: Our results showed that, although the serum levels of IL10 were lower in vitamin D deficient girls compared to control group, but this difference was not found statistically significant (P = 0.204). Conclusion: Based on our result, despite the fact that the changes of IL-10 in vitamin D deficients are not significant, it may be concluded that vitamin D deficients are not unable to express enough concentration of IL-10. This immunomodulatory and antiinflammatory activity may be efficient in immune-mediated disorders and support a therapeutic role of vitamin D in such diseases. Keywords: IL-10, Vitamin D deficiency, Girls
doi:10.1016/j.clinbiochem.2011.08.324
Poster – [A-10-238-1] C-reactive protein and Adenosine deaminase measurment for the differentiation between malignant and tuberculous pleural effusion Saeed Alinejad Moallema, Aazam Ghorbannia Delavarb a Ghazvin center, Iran b Mahmud abad Center, Iran E-mail addresses: [email protected] (S. Alinejad Moallem), [email protected] (A. Ghorbannia Delavar) Introduction: The aim of this study was to determine the validity of pleural fluid C-reactive protein (CRP) concentrations and Adenosine deaminase (ADA) activity for differentiating tuberculous pleuritis (TBP) from malignant pleural effusion (MPE) in patients presenting with lymphocytic exudative pleural effusions. Materials and methods: A cross-sectional study was conducted on 73 patients with pleural effusion who underwent diagnostic evaluation at a pulmonary and lung disease hospital in Minsk, Belarus. The CRP concentrations and ADA activity were then measured in stored sera and pleural fluid samples from patients with a lymphocytic exudative pleural effusion and with a definite diagnosis. Results: Among the 73 patients with lymphocytic exudative pleural effusions, 45 were diagnosed with TBP, 28 with MPE. Pleural fluid and serum CRP levels were significantly higher in the TBP group than in the MPE group (37.13 ± 29.32 mg L− 1 and 82.00 ± 60.99 mg L− 1 vs. 20.06 ± 18.25 mg L− 1 and 29.80 ± 23.90 mg L− 1, respectively, p < 0.05). And also pleural fluid and serum ADA levels were significantly higher in TBP group than in the MPE group (84.56 ± 37.25 U L− 1 and 35.34 ± 9.85 U L− 1 vs. 19.46 ± 10.18 U L− 1 and 18.81 ± 5.28 U L− 1, respectively, p < 0.05). Conclusion: The combination of Adenosine deaminase and Creactive protein levels might be sufficient for discriminating between the two different groups of lymphocytic exudative pleural effusion: tuberculous and malignant. However, further studies are needed for the validation of these findings in usual clinical circumstances. Keywords: C-reactive protein (CRP), Adenosine deaminase (ADA), Tuberculous pleuritis (TBP), Malignant pleural effusion (MPE)
doi:10.1016/j.clinbiochem.2011.08.325
Abstracts
Molondialdehyde (MDA) as a marker for lipid peroxdation were determined. Results: Significant increases were found in the plasma MDA level. Activities of glutathione peroxidase and glutathione reductase in plasma were decreased in OCP users compared to the control group. Conclusion: These data suggest that low-dose oral contraceptives, by enhancing the oxidative stress and lipid peroxidation, may represent a potential cardiovascular risk factor. Keywords: Stress oxidative, Lipid peroxidation, Oral contraceptives doi:10.1016/j.clinbiochem.2011.08.319
Poster – [A-10-175-2] The comparison of the level of the heat shock protein 70 between neonate with perinatal asphyxia and healthy neonate Hasan Boskabadi, Masoud Omidian, Shima Tavallai, Shabnam Mohammadi, Mostafa Parizadeh, Majid Ghayour-Mobarhan a Mashhad University of Medical Sciences, Mashhad, Iran b Biochemical and Nutritional Research Centre, Mashhad University of Medical Sciences, Mashhad, Iran E-mail address: [email protected] (M. Ghayour-Mobarhan) Introduction: Perinatal asphyxia is an important cause of death and permanent neurological and developmental complications. Early and accurate diagnosis not only prognosis but also in determining the proper treatment would be helpful, according to animal studies conducted in conditions of cellular stress, including heat and ischemia, a protein called HSP70, which appeared to be good and can be a useful marker of cellular stress and might be able to determine the neonatal asphyxia in faster, more accurate diagnosis known. Method: In this observational study, the level of HSP 70 was compared between neonates suffering from perinatal asphyxia (n= 50) and normal neonates (n= 51), whom were recruited from Ghaem hospital, Mashhad, Iran. Term neonates without major anomalies who had asphyxia during delivery were enrolled in the first six hours after delivery, and control group were healthy term neonates without problems and the delivery process during the first week of life and their cord blood during labor to measure HSP 70 by using in-house ELISA. Results: The median values of serum anti HSP70 titers were significantly higher in asphyxiated neonates compared with non asphyxiated neonates (0.36 [0.04–1.14] vs. 0.24 [0.01–0.63]. At cut off point= 0.31 AU, sensitivity was 58% and specificity was 76%. Conclusion: A significant difference between control and patient groups in the level of HSP70 was observed in this study. Also, HSP70 could be a good marker for the early diagnosis of prenatal hypoxia. Keywords: Neonatal asphyxia, Hypoxic–ischemic encephalopathy, Heat shock protein 70 doi:10.1016/j.clinbiochem.2011.08.320
Poster – [A-10-194-1] Association between RAGE (Receptor for Advanced Glycation End products) and lipid peroxidation in sperm of diabetic men Jamshid Karimia, Taghi Goodarzi Mohammada, Heidar Tavilania, Iraj Khodadadia, Iraj Amirib, Hossein Pirya a Department of Biochemistry, Hamadan University of Medical Science, Iran b Department of Anatomy, Hamadan University of Medical Science, Iran E-mail address: [email protected] (J. Karimi)
Introduction: Majority of diabetic male patients have disturbances in their reproductive systems. However, the mechanisms underlying these disturbances are largely unknown. Since receptor for advanced glycation end products (RAGE) have a key role in oxidative stress and cell damage in diabetic complications, we hypothesize that RAGE may be involved in sperm lipid peroxidation. Materials and methods: RAGE levels in sperm of 32 diabetic and 35 non-diabetic men were determined using ELISA and western blot analysis. Contents of lipid peroxidation in sperm were determined by thiobarbituric acid reaction. Results: RAGE protein levels were found significantly higher in sperm of diabetic men than non-diabetic group (p < 0.001). Moreover, sperm lipid peroxidation was significantly higher in diabetic subjects than non-diabetic men. Sperm RAGE was directly correlated to sperm lipid proxidation in diabetic men (r = 0.69, p < 0.001). Conclusions: A highly positive correlation between RAGE levels and lipid peroxidation in sperm of diabetic men may be the elucidating central role of RAGE in disturbances in sexual function of diabetic men. Keywords: RAGE, Sperm, Lipid peroxidation, Diabetes doi:10.1016/j.clinbiochem.2011.08.321
Poster – [A-10-209-1] The effects of different concentration of opium on the secretion of TGF-β in Jurkat cells Gholamreza Asadikarama, Somayeh Eigderb, Mohammad Kazemi-arababadic, Mehdi Mahmoodib a Department of Biochemistry and Physiology Research Center, Kerman University Of Medical Sciences, Kerman, Iran b Dept of Biochemistry Faculty of Medicine, Rafsanjan University of Medical Sciences, Iran c Dept of Immunology Faculty of Medicine, Rafsanjan University of Medical Sciences, Iran E-mail addresses: [email protected] (G. Asadikaram), [email protected] (S. Eigder), [email protected] (M. Kazemi-arababadi), [email protected] (M. Mahmoodi) Introduction: Transforming growth factor-β (TGF-β) is an important cytokine that has a broad spectrum of biological functions and actions in a variety of cell types. This cytokine has inhibitory effect on the immune system and diminished production of this cytokine is associated with autoimmune disorders. The aim of this study was to evaluate the influence of different concentration of opium on the secretion of TGF-β in Jurkat cells. Material and methods: In the current study, different concentrations of opium (2.85 × 10− 1, 2.85 × 10− 3, 2.85 × 10− 5, 2.85 × 10− 7, 2.85 × 10− 9, 2.85 × 10− 11 g/ml) were added to 24-well plates containing 5 × 105 Jurkat cells. The concentration of TGF-β was measured after 6, 12, 24, 48, and 72 hours (h) by ELISA . P < 0.05 was considered as the statistically significant level. Results: Our results indicated that the mean level of TGF-β in Jurkat cells was significantly higher at: 6 h in concentrations of 2.85 × 10− 1 and 2.85 × 10− 5 g/ml, at 24 and 48 h in concentrations of 2.85 × 10− 9, 2.85 × 10 − 11 g/ml. However, in concentrations of 2.85 × 10− 3, 2.85 × 10− 5, and 2.85 × 10− 7 at 48 h and concentration of 2.85 × 10− 3, 2.85 × 10− 5 and 2.85 × 10− 7 at 72 h the mean amount of TGF-β was obviously lower compared with control. Conclusion: These results showed that opium impacts immune system and cell function through a change in the level of TGF-β in T lymphocyte and this effect depends on both dose and times. This effect may play a role in immune dysfunction in opium addicts.
Abstracts
Keywords: Opium, TGF-β, Jurkat cell doi:10.1016/j.clinbiochem.2011.08.322
Poster – [A-10-223-3] Comparison of prevalence beta lactamase enzyme in bacteria isolated of clinical sample Shilla Jalalpoora, Abousaidi Hamidb a Islamic Azad University, Shahreza Branch, Shahreza, Iran b University of Medical Science, Rafsanjan, Iran E-mail address: [email protected] (S. Jalalpoor) Introduction: Beta lactame antibiotics are very important in the cure of diseases. Beta lactamase is a virulence agent and causes resistance to these antibiotics. Spread of beta lactamase in pathogen bacteria can be a disorder to cure. The aim of this search is the comparison of prevalence of beta lactamase enzyme in isolated clinical sample Bacteria in Alzahra hospital. Materials and methods: The research was performed with laboratory method in the years 1384/1385, in Alzahra hospital in Isfahan. According to statistical formula 100 infection samples (blood, urine, skin) were randomly selected and bacteria were transmitted into the laboratory. Identification of bacteria was performed with microbiological methods, for example: staining, chemical test, use of differential and selective media. For determine beta lactamase production in bacteria, use acidimetric method. All the statistical analyses were carried out using SPSS version 14. Chi-square and fisher test were used for determination of significance of association. The p ≤ 0.05 was considered significant. Results: From 100 isolated pathogen bacteria 68/31% produced beta lactamase. Respectively, these were Staphylococcus spp. 83.33%, Enterobacteriaceae spp. 70.95% and Pseudomonas 18.8%. Conclusion: The reason for the prevalence of beta lactamase enzyme in isolated bacteria of nosocamial infection, which was very high, was due to increased resistanceto beta lactam antibiotics in pathogen bacteria. Keywords: Beta lactamase, Pathogen bacteria, Clinical sample, Beta lactam antibiotics
doi:10.1016/j.clinbiochem.2011.08.323
Poster – [A-10-228-1] Serum levels of Interleukin (IL)-10 in 11–17 years old girls with vitamin D deficiency and control group Zahra Jamalia, Gholamreza Asadikarama, Mohammad Kazemi-arababadib a Dept. Of Biochemistry and Physiology Research center, Kerman University of medical Sciences, Kerman, Iran b Dept. of Microbiology, Hematology and Immunology, Faculty of Medicine and Molecular-Medicine Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran E-mail addresses: [email protected] (Z. Jamali), [email protected] (G. Asadikaram), [email protected] (M. Kazemi-arababadi) Introduction: Vitamin D not only is essential for bone health and preventing of many chronic diseases but also it recognized as an important modulator of the immune system. The aim of this study was to examine the serum levels of the IL-10, as a regulatory cytokine that prevent elevated production of inflammatory cytokines, in vitamin D deficient adolescent girls compared to control group.
S135
Materials and methods: Serum samples of 100 girls with vitamin D deficiency aged 11–17 years, and 100 healthy girls were selected and serum levels of IL-10 were measured by ELISA. Results: Our results showed that, although the serum levels of IL10 were lower in vitamin D deficient girls compared to control group, but this difference was not found statistically significant (P = 0.204). Conclusion: Based on our result, despite the fact that the changes of IL-10 in vitamin D deficients are not significant, it may be concluded that vitamin D deficients are not unable to express enough concentration of IL-10. This immunomodulatory and antiinflammatory activity may be efficient in immune-mediated disorders and support a therapeutic role of vitamin D in such diseases. Keywords: IL-10, Vitamin D deficiency, Girls
doi:10.1016/j.clinbiochem.2011.08.324
Poster – [A-10-238-1] C-reactive protein and Adenosine deaminase measurment for the differentiation between malignant and tuberculous pleural effusion Saeed Alinejad Moallema, Aazam Ghorbannia Delavarb a Ghazvin center, Iran b Mahmud abad Center, Iran E-mail addresses: [email protected] (S. Alinejad Moallem), [email protected] (A. Ghorbannia Delavar) Introduction: The aim of this study was to determine the validity of pleural fluid C-reactive protein (CRP) concentrations and Adenosine deaminase (ADA) activity for differentiating tuberculous pleuritis (TBP) from malignant pleural effusion (MPE) in patients presenting with lymphocytic exudative pleural effusions. Materials and methods: A cross-sectional study was conducted on 73 patients with pleural effusion who underwent diagnostic evaluation at a pulmonary and lung disease hospital in Minsk, Belarus. The CRP concentrations and ADA activity were then measured in stored sera and pleural fluid samples from patients with a lymphocytic exudative pleural effusion and with a definite diagnosis. Results: Among the 73 patients with lymphocytic exudative pleural effusions, 45 were diagnosed with TBP, 28 with MPE. Pleural fluid and serum CRP levels were significantly higher in the TBP group than in the MPE group (37.13 ± 29.32 mg L− 1 and 82.00 ± 60.99 mg L− 1 vs. 20.06 ± 18.25 mg L− 1 and 29.80 ± 23.90 mg L− 1, respectively, p < 0.05). And also pleural fluid and serum ADA levels were significantly higher in TBP group than in the MPE group (84.56 ± 37.25 U L− 1 and 35.34 ± 9.85 U L− 1 vs. 19.46 ± 10.18 U L− 1 and 18.81 ± 5.28 U L− 1, respectively, p < 0.05). Conclusion: The combination of Adenosine deaminase and Creactive protein levels might be sufficient for discriminating between the two different groups of lymphocytic exudative pleural effusion: tuberculous and malignant. However, further studies are needed for the validation of these findings in usual clinical circumstances. Keywords: C-reactive protein (CRP), Adenosine deaminase (ADA), Tuberculous pleuritis (TBP), Malignant pleural effusion (MPE)
doi:10.1016/j.clinbiochem.2011.08.325