- Email: [email protected]
The use of bacterial haemoglobin (VHb) expressing ethanologenic E. coli for effective ethanol fermentation from glucose
Abstracts / Journal of Biotechnology 256S (2017) S17–S43
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specific RT-PCRs. Results showed that modification of stress regime did not affect the expression rate for rbcS and rbcL coding genes; The transcript of GSTF genes was not modified in different salinity concentrations but depended on the time of exposure; Only the cultivar Dajti gave a PCR product from the amplification of a specific fragment of DREB gene. In conclusion, local cultivars display differences in the molecular mechanisms activated as a response toward stress conditions, and further research is needed to complete the knowledge on this important issue.
to investigate the use of BC nanocomposite as anode material in rechargeable batteries. In this study, BC was produced along with silicon nanoparticle addition into the growth medium of G. xylinus in six days at 30 ◦ C, dried at 60 ◦ C and carbonized at 750 ◦ C. The BC covered with silicon nanocomposite (BC-Si) showed reasonably good electrochemical performances with higher and stable discharge capacities than that of BC alone. As a result, it was determined that BC-Si could be considered as a potential anode material in Li-ion batteries.
http://dx.doi.org/10.1016/j.jbiotec.2017.06.684
Acknowledgments
Renewables, Biorefinery, Bioenergy, Biofuels, Bioproducts
This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK, 115M390).
Bioethanol production from fruit processing wastes Erman ∗ ,
Ayse Gokce Taner Sar, Gamze Seker, Meltem Yesilcimen Akbas Molecular Biology and Genetics, Gebze Technical University, Turkey E-mail address: [email protected] (A.G. Erman). Sugar rich wastes are available substrates for bioethanol production from fruit processing industry such as fruit juice, jelly tots and gums. Bioethanol is a promising alternative fuel to fossil fuels and its derivatives. Producing ethanol from carbohydrate containing food wastes can be accepted as a cost-effective process for its use since it is used either as a sole fuel or blended with gasoline. Yeasts are the best-known ethanol producer organisms. However, ethanologenic bacteria can ferment different types of sugars. In this study, the fruit processing waste was hydrolysed by using dilute acid to fermentable sugars. Ethanologenic E. coli strain FBR5 was grown in the fermentation medium supplemented with hydrolysate of fruit processing waste as a carbon resource which contains 3.6% (w/v) fructose for 48 h. After 24 and 48 h incubation, 1.38% (w/v) and 1.57% (w/v) ethanol is produced, respectively by E. coli FBR5 strain. It was shown that the use of fruit processing waste for ethanol production can be a cost effective and a safe process. This approach can be used for large scale production since the raw fruit waste is generated from fruit industries. http://dx.doi.org/10.1016/j.jbiotec.2017.06.685 The use of bacterial cellulose nanocomposites as an electrode material in Lithium ion batteries Dursun 1 ,
Sar 2,∗ ,
Erman 2 ,
Burcu Taner Ayse Gokce Gamze Seker 2 , Rezan Demir Cakan 3 , Meltem Yesilcimen Akbas 2
http://dx.doi.org/10.1016/j.jbiotec.2017.06.686 The use of bacterial haemoglobin (VHb) expressing ethanologenic E. coli for effective ethanol fermentation from glucose Gamze Seker ∗ , Taner Sar, Ayse GOkce Erman, Meltem Yesilcimen Akbas Department of Molecular Biology and Genetics, Gebze Technical University, Kocaeli, Turkey E-mail address: [email protected] (G. Seker). Ethanol production by microbial fermentation to supply increasing energy demand has become attractive. In recent years, there has been growing interest for producing bioethanol from agricultural products or their wastes which contains various sugar types specifically continuous ethanol production by using microorganisms for improved for bioethanol production. Vitreoscilla hemoglobin (VHb) is the first characterized prokaryotic haemoglobin. The heterologous expression of VHb in many bacteria increased cellular growth and production of many industrial metabolites. In this study, free and alginate-immobilized ethanologenic E. coli strains FBR5 and TS4 (vgb+) were used to asses effective ethanol production in growth medium supplemented with glucose (8%) as a monosaccharide found in starch or lignocellulosic hydrolysate after 48 h fermentation. It was shown that the immobilized TS4 (vgb+) is more efficient than free FBR5 cells in ethanol production from glucose containing medium after 48 h. Immobilization in combination with Vhb expression could be a promising tool for effective ethanol production from sugars. http://dx.doi.org/10.1016/j.jbiotec.2017.06.687 Biostimulation and bioaugmentation of microbial community involved in the biogas production of sewage sludge
1
Material Science and Engineering, Gebze Technical University, Kocaeli, Turkey 2 Molecular Biology and Genetics, Gebze Technical University, Kocaeli, Turkey 3 Chemical Engineering, Gebze Technical University, Kocaeli, Turkey E-mail address: [email protected] (T. Sar). Bacterial cellulose (BC) is the most abundant biopolymer produced by G. xylinus. BC has been used as biomaterial in biomedical applications such as artificial blood vessels, wound healing and tissue regeneration and a stabilizing agent and gelling agent in food industry. BC has been also used for restoration of old papers, using as food packing and as conductors in electrical practices. This work aimed
Martyna Wojcieszak 1,∗ , Lukasz Dziewit 2 , Lukasz Drewniak 1 1 Laboratory of Environmental Pollution Analysis, Faculty of Biology, University of Warsaw, Poland 2 Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Poland
E-mail address: [email protected] (M. Wojcieszak). Anaerobic digestion (AD) is a complex process carried out by a number of microorganisms whose activities are determined by a variety of physicochemical parameters, as well as the availability of the nutrient substrates and growth supplements. The objective of this
S41
specific RT-PCRs. Results showed that modification of stress regime did not affect the expression rate for rbcS and rbcL coding genes; The transcript of GSTF genes was not modified in different salinity concentrations but depended on the time of exposure; Only the cultivar Dajti gave a PCR product from the amplification of a specific fragment of DREB gene. In conclusion, local cultivars display differences in the molecular mechanisms activated as a response toward stress conditions, and further research is needed to complete the knowledge on this important issue.
to investigate the use of BC nanocomposite as anode material in rechargeable batteries. In this study, BC was produced along with silicon nanoparticle addition into the growth medium of G. xylinus in six days at 30 ◦ C, dried at 60 ◦ C and carbonized at 750 ◦ C. The BC covered with silicon nanocomposite (BC-Si) showed reasonably good electrochemical performances with higher and stable discharge capacities than that of BC alone. As a result, it was determined that BC-Si could be considered as a potential anode material in Li-ion batteries.
http://dx.doi.org/10.1016/j.jbiotec.2017.06.684
Acknowledgments
Renewables, Biorefinery, Bioenergy, Biofuels, Bioproducts
This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK, 115M390).
Bioethanol production from fruit processing wastes Erman ∗ ,
Ayse Gokce Taner Sar, Gamze Seker, Meltem Yesilcimen Akbas Molecular Biology and Genetics, Gebze Technical University, Turkey E-mail address: [email protected] (A.G. Erman). Sugar rich wastes are available substrates for bioethanol production from fruit processing industry such as fruit juice, jelly tots and gums. Bioethanol is a promising alternative fuel to fossil fuels and its derivatives. Producing ethanol from carbohydrate containing food wastes can be accepted as a cost-effective process for its use since it is used either as a sole fuel or blended with gasoline. Yeasts are the best-known ethanol producer organisms. However, ethanologenic bacteria can ferment different types of sugars. In this study, the fruit processing waste was hydrolysed by using dilute acid to fermentable sugars. Ethanologenic E. coli strain FBR5 was grown in the fermentation medium supplemented with hydrolysate of fruit processing waste as a carbon resource which contains 3.6% (w/v) fructose for 48 h. After 24 and 48 h incubation, 1.38% (w/v) and 1.57% (w/v) ethanol is produced, respectively by E. coli FBR5 strain. It was shown that the use of fruit processing waste for ethanol production can be a cost effective and a safe process. This approach can be used for large scale production since the raw fruit waste is generated from fruit industries. http://dx.doi.org/10.1016/j.jbiotec.2017.06.685 The use of bacterial cellulose nanocomposites as an electrode material in Lithium ion batteries Dursun 1 ,
Sar 2,∗ ,
Erman 2 ,
Burcu Taner Ayse Gokce Gamze Seker 2 , Rezan Demir Cakan 3 , Meltem Yesilcimen Akbas 2
http://dx.doi.org/10.1016/j.jbiotec.2017.06.686 The use of bacterial haemoglobin (VHb) expressing ethanologenic E. coli for effective ethanol fermentation from glucose Gamze Seker ∗ , Taner Sar, Ayse GOkce Erman, Meltem Yesilcimen Akbas Department of Molecular Biology and Genetics, Gebze Technical University, Kocaeli, Turkey E-mail address: [email protected] (G. Seker). Ethanol production by microbial fermentation to supply increasing energy demand has become attractive. In recent years, there has been growing interest for producing bioethanol from agricultural products or their wastes which contains various sugar types specifically continuous ethanol production by using microorganisms for improved for bioethanol production. Vitreoscilla hemoglobin (VHb) is the first characterized prokaryotic haemoglobin. The heterologous expression of VHb in many bacteria increased cellular growth and production of many industrial metabolites. In this study, free and alginate-immobilized ethanologenic E. coli strains FBR5 and TS4 (vgb+) were used to asses effective ethanol production in growth medium supplemented with glucose (8%) as a monosaccharide found in starch or lignocellulosic hydrolysate after 48 h fermentation. It was shown that the immobilized TS4 (vgb+) is more efficient than free FBR5 cells in ethanol production from glucose containing medium after 48 h. Immobilization in combination with Vhb expression could be a promising tool for effective ethanol production from sugars. http://dx.doi.org/10.1016/j.jbiotec.2017.06.687 Biostimulation and bioaugmentation of microbial community involved in the biogas production of sewage sludge
1
Material Science and Engineering, Gebze Technical University, Kocaeli, Turkey 2 Molecular Biology and Genetics, Gebze Technical University, Kocaeli, Turkey 3 Chemical Engineering, Gebze Technical University, Kocaeli, Turkey E-mail address: [email protected] (T. Sar). Bacterial cellulose (BC) is the most abundant biopolymer produced by G. xylinus. BC has been used as biomaterial in biomedical applications such as artificial blood vessels, wound healing and tissue regeneration and a stabilizing agent and gelling agent in food industry. BC has been also used for restoration of old papers, using as food packing and as conductors in electrical practices. This work aimed
Martyna Wojcieszak 1,∗ , Lukasz Dziewit 2 , Lukasz Drewniak 1 1 Laboratory of Environmental Pollution Analysis, Faculty of Biology, University of Warsaw, Poland 2 Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Poland
E-mail address: [email protected] (M. Wojcieszak). Anaerobic digestion (AD) is a complex process carried out by a number of microorganisms whose activities are determined by a variety of physicochemical parameters, as well as the availability of the nutrient substrates and growth supplements. The objective of this