Toll-like receptor 5 is a prognostic marker in oral tongue cancer

OOOO Volume 114, Number 4 SQUAMOUS CELL CARCINOMA OF THE TONGUE: A HISTOLOGICAL STUDY OF 57 CASES Silva-Filho TJ1, Vasconcelos MG, Vasconcelos RG, Silveira EJD, Queiroz LMG - 1UFRN Background: Oral squamous cell carcinoma is a malignant tumor of epithelial origin that accounts more than 90% of the cancers occurring in the oral cavity. Objective: evaluate and correlate the incidence of clinical and morphological data on cases of tongue squamous cell carcinoma (TSCC). Study design: it was selected 57 tissue specimens of TSCC, embedded in paraffin, cut into 5mm thickness, stained with hematoxylin-eosin and subjected to histological studies using light microscopy. A statistic analysis of pathological features and histological grading of malignancy due to invasion of the lesions was made (Qui2 test). Results: we found that metastasis was statistically correlated to the upshot disease (p⫽0.000) and clinical stage (p⫽0.001). The relationship between clinical staging and histological grading showed that there were significant differences between these groups analyzed (p⫽0.006). Conclusion: the clinical staging associated with the histological grading can be a predictive tool for the information of prognosis of TSCC.

TOLL-LIKE RECEPTOR 5 IS A PROGNOSTIC MARKER IN ORAL TONGUE CANCER Kauppila JH1,2, Mattila AE1, Karttunen TJ1,3, Salo T4,3 - 1UNIV OF OULU, OULU, FINLAND - DEPARTMENT OF PATHOLOGY, 2DEPARTMENT OF ANATOMY AND CELL BIOLOGY, 3OULU UNIV HOSPITAL, OULU, FINLAND, 4UNIV OF OULU, OULU, FINLAND - INSTITUTE OF DENTISTRY Introduction: Toll-like receptor 5 (TLR5) is an innate immune receptor recognizing bacterial flagellin. TLR5 activation has been shown to affect cancer invasion. Considering that oral bacterial flora may be involved in malignant transformation, we studied TLR5 expression in oral tongue squamous cell carcinoma (OTSCC) and its association with clinical outcome. Study design: TLR5 was analyzed in 119 patients with OTSCC using immunohistochemistry. The staining intensities and the percentage of positive cells were assessed. For statistical analyses Chi-square-test, the Kaplan-Meier method and Cox proportional hazards model were used. Results: TLR5 expression was increased in OTSCC compared to normal epithelium (p⬍0.001). High TLR5 expression was associated to reduced disease-free survival (p⬍0.001) and increased cancer mortality (p⬍0.01). Conclusion: TLR5 has a role in the pathophysiology of OTSCC and might represent a link between bacterial exposure and cancer. TLR5 could be considered as a marker for predicting recurrence or survival of OTSCC patients.

A SURVEY OF ORAL AND MAXILLOFACIAL PATHOLOGY SPECIMENS FROM A BRAZILIAN POPULATION OVER AN 11-YEAR PERIOD Mariano VC1, Souza LS1, Fernandes KS1, Ito FA1 - 1UNIVERSIDADE ESTADUAL DE LONDRINA Objective: Epidemiological surveys of oral lesions are important to be aware of the incidence, prevalence and distribution of various oral diseases. This study carried out a survey of oral and maxillofacial pathology specimens submitted for diagnosis.

ORAL ABSTRACT Abstracts e135 Study design: All entries for specimens during the 11-year period from 1998 to 2008 were retrieved and compiled into 11 diagnostic categories. Results: During the study period, 3090 specimens were analysed. The diagnostic category with the largest number of specimens was mucosal pathology (56.5%), followed by salivary gland disease excluding neoplasms (10.7%) and odontogenic cysts and tumours (6.6%). Benign tumours accounted for 6.6% of all specimens and malignant tumours 4.7%. Fibrous hyperplasia was the most frequent diagnosis (48.3%), followed by mucocele (9.7%), squamous papilloma (3.9%) and squamous cell carcinoma (3.8%). Conclusion: This study showed that most of the diagnoses are benign, however we cannot ignore the amount of malignant tumours, particularly squamous cell carcinoma.

PRKAR1A: A POSSIBLE LINK TO AMELOBLASTIC FIBROMA PATHOGENESIS Bernardes VF1, Sousa SF1, Correa GTB1, Gomes CC2, Gomez RS1 - 1FACULDADE DE ODONTOLOGIA - UNIVERSIDADE FEDERAL DE MINAS GERAIS - CLÍNICA, PATOLOGIA E CIRURGIA ODONTOLÓGICAS, 2INSTITUTO DE CIÊNCIAS BIOLÓGICAS UNIVERSIDADE FEDERAL DE MINAS GERAIS - PATOLOGIA Background: Ameloblastic fibroma is an uncommon odontogenic tumour with aggressive behavior and potential for malignant transformation. Despite studies investigating the pathogenesis of odontogenic tumours, the origin of the ameloblastic fibroma is still uncertain. The identification of molecules involved in the mechanisms of development of this lesion requires further exploration. The PRKAR1A is a suppressor gene enrolled in cell differentiation and proliferation. Objective: The aim of this study was to investigate the immunohistochemical expression of the protein PRKAR1A and the loss of heterozygosis (LOH) in ameloblastic fibroma. Study design: The PRKAR1A expression was analysed by immunohistochemistry and the LOH was assessed by 5 microsatellite markers: D17S2182, D17S1882, D17S940, D17S942, and PRKAR1A in paraffin-embedded samples of ameloblastic fibroma. Results: Lack of protein expression in the ectomesenchymal component and LOH were identified in both epithelial and ectomesenchymal components. Conclusion: The results indicate that PRKAR1A could be related to the pathogenesis of this odontogenic tumour. This study was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil.

IMMUNOHISTOCHEMICAL EVALUATION OF ANGIOGENESIS AND TRYPTASE-POSITIVE MAST CELLS INFILTRATION IN PRIMARY NODAL HODGKIN LYMPHOMA AND PRIMARY ORAL DIFFUSE LARGE B-CELL LYMPHOMA WITH HODGKIN/REED-STERNBERG-LIKE CELLS Toral-Rizo VH1, Carlos-Bregni R2, Delgado-Azañero W3, Mosqueda-Taylor A4, Soares FA5, Vassallo J6, Almeida OP7 1 PIRACICABA DENTAL SCHOOL, UNIVERSITY OF CAMPINAS-UNICAMP - ORAL PATHOLOGY SECTION, DEPARTMENT OF ORAL DIAGNOSIS, 2CENTRO CLÍNICO DE CABEZA Y CUELLO, GUATEMALA CITY, GUATEMALA, 3 FACULTAD DE ESTOMATOLOGIA, UNIVERSIDAD CAY-