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Ultraviolet B radiation induces increased proliferation and melanization of aged melanocytes compared with young cells
60
Poster Presenlalions
279
282
280
283
ULTRAVIOLET B RADIATION INDUCES INCREASED PROLIFERATION AND MELANIZATION OF AGED HELANGCYTES COMPARED WITH YOUNG CELLS. oko Funasaka, Aklko Qhah~. !&da and Hv %~,i:::6,;:satQ
CULTURED
Kobe University With aging. such
as
choolof Med;cine. sun exposure Induces
solar
ultraviolet proliferation
lentigine.
cell
The doubling twice longer
strains
time than
of melanocytes markedly after -old
boy
were
also
the
to
from culture,
grow
stably
melanocytes darker
observed in
melanocytes
foreskins
of melanocytes that of 4 years
continue
BY 2.5 mJ/cn2 increased in
from
established 3 months
viva. and
melanocytes aged
examine
are
long indicating
inducible
IJVB. ‘H-thymidine aged melanocytes.
of
aged
of older of age.
more more
4
to
than
than
6
that uptake These
“f
Med.
Kawsak1.
“Bmloalcal
,nsteute.
Dermatolwy. St. Marlanna Umv School FaC”ltY of Saence. Tohaku “III”. Senda
TO Study a role of c kit I" melanocyted,ffere"tlatla". IxlrnXY "e"ralcrest cell cultureswere prepared from E9.5 C57BL,6 mouse embryos. and c kit
years.
expressran on the cultured cells was lmmunoh,stochemlcally exammed by “smg a monoclonal anti-c-kit antibody (ACKZ, Nishlkawa et ai 1991) cultured day 3. 6. 9. 15. One neural tube was placed on a &sh and cultured m MEM medium wth or without TPA and cholera toxm. The c-kit expressmn was noted on day 3 and was mamly ,nduced by TPA. and asslsted by cholera tax,“. From th,er dendntx appearance of c-kit positive cells, it was assumed that mast of the wsltlve cells mlgbt be melanocytes. Most of these cells beeme dopa-pas~tive an day 9. Namely. melanocytes express c-k,t on tber cell surface before they d,fferentmted into dopa ~os,t,ve cells. Therefore. ,t is suggested that c kit exwessmn may play a role I” rwamocytes dlfferentlatian.
years
growth
IS speed
decreased of I-year
months.
With
larger
characteristics
cultivation after
and
Masako Mlzoguchl”. Hlrotake 0”““. “L%pt.of
at
52
20
The
dendrites.
These
aging
and normal
52-year-old male while meianocytes
pigmentalon. after
vitro.
have
lesion
effect
radiation on growth. configuration of melanocytes. we established
nelanocyte
aging in cytoplasm
To
Kobe. Japan hyperpigmented
OF MELANOCYTES AND EXPRESSION OF C KIT IN 1HE NEURAL. CR%T CELLS. MltsuhiroSate".Y&o Kawa". Yasuo Kubata'.
DIFFERENTIATION
of
I-year-old
characteristics certain
cell
of divisions.
and pigmentation results indicate
that
aged skin has a potency of hyperpigmentatlon by UVB radiation. through efficient melanocyte dlvlsion and nelanogenesis.
281
284
THE INCIDENCE OF NIJCLE%K CAPS IN DYSCHROMIA. LAnda &&&, Department of Dermatology, University of Wales College of Medicine, Heath Park, &rdifF, U.K. Nuclear caps am area of melanin seen above the nucleus of basal cells. It ie thought that they are derived from the pbagocytasis of melanosomes or melanin granules, and they may have B timction of providing wme protection to the nucleus fmm W irradiation. We have developed a new technique for the analysis of melanin content of skin and its morphology in surface mrneocytea by examination of tape strips from the skin surface. Using a haemataxylin end silver nitrate staining method with image analysie we have detected melanin in surfam conmocyte~ in a similar distribution to that observed 88 nuclear cape in basal keratinwyta. In a gmup of 24 normal volunteers we found little evidence of nuclear cape in mrneccytes ftnm unexpoead sites, and a small incidence in more exposed areas. In a group of 20 patients with senile lent& and melanc&ic naevi. we found a very high incidenar of nuclear cap-like melanin distribution in aurfacz corneoeytas fmm these leeions. In 5 patients with vitiliga we found not only no nuclear caplike structures, but no melanin whatsoever (Table 11. Fmm the data obtained, it could be that nuclear cap structures in corneacytes ret&t an abnornmlity in melanccytes at the sites examined, and their presence may be useful in characterising pigmentary ammmlies.
CHANGE IN MELANOCYTES OF NEWBORN MICE BY ADMINISTRATION OF A MONCCLONA,. ANTICKIT ANTIBODY (ACKZ) Okura. Masako Mxo~uchi. Department of Dermatology. St. Mananna University School of Mednne, Kawasaki. Japan We examined the develow,ent of melanocytes m newborn rmce hair folhclles by pentoneal m,ectxm of a monoclonal ant>-c-kit antIbody (ACKZ). Melanoc~tes m the falhcles. ep&rrms. and dernus were studled by light and electron mlCrOscOplc examlnatlons dopa reaction. dwa and mmwnoh~stochermcal exammatmns before and after ACKZ (kmdly presented by Prof. Nishlkawa. Kumamoto Umverslty) mjectmn on days 0. 2. 4. 6. 8 past-partum. In normal untreated mice. melanecytes become actxve m the epidermis after bxth and the number of dapa-positwe meianocytes has mcreased UP to day 4 post parturn. They gradually mwrate from the epidermis to the hax follicles accordmg to the development of the follicles. Thus, the number of wdermal melanocvtes decrease after day 4 LXX-~artum. In ACKZ treated rruce. the earher the nnce received the ACK2 mjection after bxth. the fewer melanocytes they had not only in the epidermis. but also in ther follicles. Destruction of dermal melamxytes on day 2 post-parturn and the destructmn of hair folhcle melanocytes on day 3 post -parturn were ultramicroscopically detected Decrease in the number of dwapositive melanocytes of bar folbcles and epldermls obtained from mice admmlsterted with ACKZ immedntely after brth. was most srgmfxant. We thus assume that m newborn nuce. the period of melanocytes’ c - kit dependency LS when melanocytes become active m the eixdermz and migrate from the widernus to the bar follicles.
a yitiliep 0.0
Incidence of nuclear cap-like melanin distribution in au&x corrleocytea per lcoo e&wwytes. Lfa&-axwsed 39.6 f 16.7
7.5 * 11.0
0.8 * 2.1
Mhsuhro
electronm~crosco,,~c
Poster Presenlalions
279
282
280
283
ULTRAVIOLET B RADIATION INDUCES INCREASED PROLIFERATION AND MELANIZATION OF AGED HELANGCYTES COMPARED WITH YOUNG CELLS. oko Funasaka, Aklko Qhah~. !&da and Hv %~,i:::6,;:satQ
CULTURED
Kobe University With aging. such
as
choolof Med;cine. sun exposure Induces
solar
ultraviolet proliferation
lentigine.
cell
The doubling twice longer
strains
time than
of melanocytes markedly after -old
boy
were
also
the
to
from culture,
grow
stably
melanocytes darker
observed in
melanocytes
foreskins
of melanocytes that of 4 years
continue
BY 2.5 mJ/cn2 increased in
from
established 3 months
viva. and
melanocytes aged
examine
are
long indicating
inducible
IJVB. ‘H-thymidine aged melanocytes.
of
aged
of older of age.
more more
4
to
than
than
6
that uptake These
“f
Med.
Kawsak1.
“Bmloalcal
,nsteute.
Dermatolwy. St. Marlanna Umv School FaC”ltY of Saence. Tohaku “III”. Senda
TO Study a role of c kit I" melanocyted,ffere"tlatla". IxlrnXY "e"ralcrest cell cultureswere prepared from E9.5 C57BL,6 mouse embryos. and c kit
years.
expressran on the cultured cells was lmmunoh,stochemlcally exammed by “smg a monoclonal anti-c-kit antibody (ACKZ, Nishlkawa et ai 1991) cultured day 3. 6. 9. 15. One neural tube was placed on a &sh and cultured m MEM medium wth or without TPA and cholera toxm. The c-kit expressmn was noted on day 3 and was mamly ,nduced by TPA. and asslsted by cholera tax,“. From th,er dendntx appearance of c-kit positive cells, it was assumed that mast of the wsltlve cells mlgbt be melanocytes. Most of these cells beeme dopa-pas~tive an day 9. Namely. melanocytes express c-k,t on tber cell surface before they d,fferentmted into dopa ~os,t,ve cells. Therefore. ,t is suggested that c kit exwessmn may play a role I” rwamocytes dlfferentlatian.
years
growth
IS speed
decreased of I-year
months.
With
larger
characteristics
cultivation after
and
Masako Mlzoguchl”. Hlrotake 0”““. “L%pt.of
at
52
20
The
dendrites.
These
aging
and normal
52-year-old male while meianocytes
pigmentalon. after
vitro.
have
lesion
effect
radiation on growth. configuration of melanocytes. we established
nelanocyte
aging in cytoplasm
To
Kobe. Japan hyperpigmented
OF MELANOCYTES AND EXPRESSION OF C KIT IN 1HE NEURAL. CR%T CELLS. MltsuhiroSate".Y&o Kawa". Yasuo Kubata'.
DIFFERENTIATION
of
I-year-old
characteristics certain
cell
of divisions.
and pigmentation results indicate
that
aged skin has a potency of hyperpigmentatlon by UVB radiation. through efficient melanocyte dlvlsion and nelanogenesis.
281
284
THE INCIDENCE OF NIJCLE%K CAPS IN DYSCHROMIA. LAnda &&&, Department of Dermatology, University of Wales College of Medicine, Heath Park, &rdifF, U.K. Nuclear caps am area of melanin seen above the nucleus of basal cells. It ie thought that they are derived from the pbagocytasis of melanosomes or melanin granules, and they may have B timction of providing wme protection to the nucleus fmm W irradiation. We have developed a new technique for the analysis of melanin content of skin and its morphology in surface mrneocytea by examination of tape strips from the skin surface. Using a haemataxylin end silver nitrate staining method with image analysie we have detected melanin in surfam conmocyte~ in a similar distribution to that observed 88 nuclear cape in basal keratinwyta. In a gmup of 24 normal volunteers we found little evidence of nuclear cape in mrneccytes ftnm unexpoead sites, and a small incidence in more exposed areas. In a group of 20 patients with senile lent& and melanc&ic naevi. we found a very high incidenar of nuclear cap-like melanin distribution in aurfacz corneoeytas fmm these leeions. In 5 patients with vitiliga we found not only no nuclear caplike structures, but no melanin whatsoever (Table 11. Fmm the data obtained, it could be that nuclear cap structures in corneacytes ret&t an abnornmlity in melanccytes at the sites examined, and their presence may be useful in characterising pigmentary ammmlies.
CHANGE IN MELANOCYTES OF NEWBORN MICE BY ADMINISTRATION OF A MONCCLONA,. ANTICKIT ANTIBODY (ACKZ) Okura. Masako Mxo~uchi. Department of Dermatology. St. Mananna University School of Mednne, Kawasaki. Japan We examined the develow,ent of melanocytes m newborn rmce hair folhclles by pentoneal m,ectxm of a monoclonal ant>-c-kit antIbody (ACKZ). Melanoc~tes m the falhcles. ep&rrms. and dernus were studled by light and electron mlCrOscOplc examlnatlons dopa reaction. dwa and mmwnoh~stochermcal exammatmns before and after ACKZ (kmdly presented by Prof. Nishlkawa. Kumamoto Umverslty) mjectmn on days 0. 2. 4. 6. 8 past-partum. In normal untreated mice. melanecytes become actxve m the epidermis after bxth and the number of dapa-positwe meianocytes has mcreased UP to day 4 post parturn. They gradually mwrate from the epidermis to the hax follicles accordmg to the development of the follicles. Thus, the number of wdermal melanocvtes decrease after day 4 LXX-~artum. In ACKZ treated rruce. the earher the nnce received the ACK2 mjection after bxth. the fewer melanocytes they had not only in the epidermis. but also in ther follicles. Destruction of dermal melamxytes on day 2 post-parturn and the destructmn of hair folhcle melanocytes on day 3 post -parturn were ultramicroscopically detected Decrease in the number of dwapositive melanocytes of bar folbcles and epldermls obtained from mice admmlsterted with ACKZ immedntely after brth. was most srgmfxant. We thus assume that m newborn nuce. the period of melanocytes’ c - kit dependency LS when melanocytes become active m the eixdermz and migrate from the widernus to the bar follicles.
a yitiliep 0.0
Incidence of nuclear cap-like melanin distribution in au&x corrleocytea per lcoo e&wwytes. Lfa&-axwsed 39.6 f 16.7
7.5 * 11.0
0.8 * 2.1
Mhsuhro
electronm~crosco,,~c