VOL. 35, NO. 1

VOL. 35, NO. 1 Editorial ........................................................................................................................................................................................................................................................... v

A new web site for the JOURNAL

J. Rode´s

The Bridge ........................................................................................................................................................................................................................................................... 1

The Bridge – July 2001

J. Reichen

Original Articles Biliary Tract and Cholestasis ...........................................................................................................................................................................................................................................................

2

Ductular morphogenesis and functional polarization of normal human biliary epithelial cells in three-dimensional culture

Y. Ishida, S. Smith, L. Wallace, T. Sadamoto, M. Okamoto, M. Auth, M. Strazzabosco, L. Fabris, J. Medina, J. Prieto, A. Strain, J. Neuberger, R. Joplin (UK, Germany, Italy, Spain)

motility and presence of solitary stones delay rather than prevent gallstone recurrence after extracorporeal shock wave lithotripsy

M.F.J. Stolk, A. Vos, P.W. Plaisier, K.J. van Erpecum (The Netherlands, Italy)

........................................................................................................................................................................................................................................................... N.G. Venneman, G.P. vanBerge-Henegouwen, P. Portincasa, 10 Absence of apolipoprotein E4 genotype, good gallbladder

Human study Cell Biology, Metabolism and Transport ...........................................................................................................................................................................................................................................................

17

Diabetic KKA y mice exhibit increased hepatic PPARg1 gene expression and develop hepatic steatosis upon chronic treatment with antidiabetic thiazolidinediones

M. Bedoucha, E. Atzpodien, U.A. Boelsterli (Switzerland)

Chronic Liver Diseases ...........................................................................................................................................................................................................................................................

24

Autoimmune hepatitis in Brazilian patients is not linked to tumor necrosis factor a polymorphisms at position 2 308

Human study

P.L. Bittencourt, S.A. Pala´cios, E.L. Rachid Canc¸ado, G. Porta, S. Drigo, F.J. Carrilho, A.A. Laudanna, J. Kalil, A.C. Goldberg (Brazil, USA)

See Editorial, pages 127–129

Cirrhosis and its Complications ...........................................................................................................................................................................................................................................................

29

Altered endothelin receptor expression in prehepatic portal hypertension predisposes the liver to microcirculatory dysfunction in rats

Y. Yokoyama, A. Wawrzyniak, R. Baveja, N. Sonin, M.G. Clemens, J.X. Zhang (USA)

electroencephalogram (EEG) alterations in cirrhotic patients

R. Iemmolo, M. Muraca, C. Musto, G. Gerunda, C. Rizzo, C. Merkel, A. Gatta (Italy)

........................................................................................................................................................................................................................................................... 37 Prevalence and prognostic value of quantified P. Amodio, F. Del Piccolo, E. Petteno`, D. Mapelli, P. Angeli,

Human study Liver Cell Injury and Liver Failure ...........................................................................................................................................................................................................................................................

46

The antiestrogen toremifene protects against alcoholic liver injury in female rats

H.A. Ja¨rvela¨inen, T.A. Lukkari, S. Heinaro, H. Sippel, K.O. Lindros (Finland)

See Editorial, pages 130–133

........................................................................................................................................................................................................................................................... 53 A non-toxic heat shock protein 70 inducer, S. Ikeyama, K. Kusumoto, H. Miyake, K. Rokutan, S. Tashiro geranylgeranylacetone, suppresses apoptosis of cultured rat hepatocytes caused by hydrogen peroxide and ethanol

(Japan)

Liver Growth and Cancer ...........................................................................................................................................................................................................................................................

62

p16 INK4A – alterations in primary angiosarcoma of the liver

Human study

A. Tannapfel, M. Weihrauch, M. Benicke, D. Uhlmann, J. Hauss, R. Wrbitzky, C. Wittekind (Germany)

...........................................................................................................................................................................................................................................................

68

Activation of c-Src gene product in hepatocellular carcinoma is highly correlated with the indices of early stage phenotype

Y. Ito, H. Kawakatsu, T. Takeda, M. Sakon, H. Nagano, T. Sakai, E. Miyoshi, K. Noda, M. Tsujimoto, K.-i. Wakasa, M. Monden, N. Matsuura (Japan, USA, Sweden)

Human study

........................................................................................................................................................................................................................................................... 74 The role of overexpression and gene amplification of cyclin K. Sugimachi, S.-i. Aishima, K.-i. Taguchi, S. Tanaka, D1 in intrahepatic cholangiocarcinoma

M. Shimada, K. Kajiyama, K. Sugimachi, M. Tsuneyoshi (Japan)

Human study

........................................................................................................................................................................................................................................................... V. Sa´ nchez-Margalet, C. Gonza´ lez-Yanes, S. Najib (Spain) 80 Pancreastatin, a chromogranin A-derived peptide, inhibits DNA and protein synthesis by producing nitric oxide in HTC rat hepatoma cells Transplantation and Surgery

........................................................................................................................................................................................................................................................... R. Zachoval, A.L. Gerbes, P. Schwandt, K.G. Parhofer 86 Short-term effects of statin therapy in patients with hyperlipoproteinemia after liver transplantation: results of a randomized cross-over trial

(Germany)

Human study Viral Hepatitis ...........................................................................................................................................................................................................................................................

92

Emergence of YMDD motif mutant of hepatitis B virus during short-term lamivudine therapy in South Korea

Y.H. Paik, H.Y. Chung, W.-S. Ryu, K.S. Lee, J.S. Lee, J.H. Kim, C.K. Lee, C.Y. Chon, Y.M. Moon, K.-H. Han (South Korea)

Human study

........................................................................................................................................................................................................................................................... 99 Transmission of hepatitis B virus infection in Gambian U. Dumpis, E.C. Holmes, M. Mendy, A. Hill, M. Thursz, families revealed by phylogenetic analysis

A. Hall, H. Whittle, P. Karayiannis (UK, Gambia)

Human study

........................................................................................................................................................................................................................................................... 105 T-helper cell response to woodchuck hepatitis virus antigens S. Herva´ s-Stubbs, J.J. Lasarte, P. Sarobe, I. Vivas, L. Condreay, after therapeutic vaccination of chronically-infected animals treated with lamivudine

J.M. Cullen, J. Prieto, F. Borra´ s-Cuesta (Spain, USA)

patients with fulminant and self-limited acute type A hepatitis

M. Omata (Japan)

........................................................................................................................................................................................................................................................... 112 Analysis of full-length hepatitis A virus genome in sera from K. Fujiwara, O. Yokosuka, K. Fukai, F. Imazeki, H. Saisho,

Human study

........................................................................................................................................................................................................................................................... 120 Changes in histological lesions and serum fibrogenesis V. Leroy, C. De Traversay, R. Barnoud, J.-D. Hartmann, markers in chronic hepatitis C patients non-responders to interferon alpha

M. Baud, D. Ouzan, J.-P. Zarski (France)

Human study

Editorials ........................................................................................................................................................................................................................................................... 127

Unravelling complexity – the scientific basis of genetic predisposition in autoimmune liver disease

U. Spengler (Germany)

........................................................................................................................................................................................................................................................... 130 Alcoholic liver disease: a matter of hormones? H. Moshage (The Netherlands) ...........................................................................................................................................................................................................................................................

Review ........................................................................................................................................................................................................................................................... 134

Ursodeoxycholic acid ‘mechanisms of action and clinical use in hepatobiliary disorders’

K.N. Lazaridis, G.J. Gores, K.D. Lindor (USA)

Images in Hepatology ........................................................................................................................................................................................................................................................... 147

Acute schistosoma mansoni infection in the liver

K. Murata, K. Shiraki (Japan)

Letters to the Editor ........................................................................................................................................................................................................................................................... 148

Heterozygosity for a polymorphism in the promoter region of the UGT1A1 gene

M. Kaplan, C. Hammerman, E. Beutler (Israel, USA)

........................................................................................................................................................................................................................................................... 149 Reply W.H.M. Peters (The Netherlands) ........................................................................................................................................................................................................................................................... 149 Reply C.P. Strassburg, M.P. Manns (Germany) ........................................................................................................................................................................................................................................................... 150 The intrahepatic T helper cytokine profile does not predict R. Quadri, E. Giostra, A. Hadengue, F. Negro (Switzerland) the response to a-interferon in chronic hepatitis C

Book Review ........................................................................................................................................................................................................................................................... 152

Liver Biopsy Interpretation, 6th edition

M. Bruguera (Spain)

Announcements ........................................................................................................................................................................................................................................................... 153

VOL. 35, NO. 2 The Bridge ........................................................................................................................................................................................................................................................... 155

The Bridge – August 2001

J. Reichen

Original Articles Biliary Tract and Cholestasis ...........................................................................................................................................................................................................................................................

156

Scavenger cells with Gram-positive bacterial lipoteichoic acid infiltrate around the damaged interlobular bile ducts of primary biliary cirrhosis

K. Tsuneyama, K. Harada, N. Kono, K. Hiramatsu, Y. Zen, Y. Sudo, M.E. Gershwin, M. Ikemoto, H. Arai, Y. Nakanuma (Japan, USA)

Human study

........................................................................................................................................................................................................................................................... C.P. Nibbering, A.K. Groen, R. Ottenhoff, J.F.H.M. Brouwers, 164 Regulation of biliary cholesterol secretion is independent of hepatocyte canalicular membrane lipid composition: a study in the diosgenin-fed rat model

G.P. vanBerge-Henegouwen, K.J. van Erpecum (The Netherlands)

Cell Biology, Metabolism and Transport ...........................................................................................................................................................................................................................................................

170

Expression and DNA-binding activity of C/EBPa and C/ EBPb in human liver and differentiated primary hepatocytes

J.-B. Ferrini, E. Rodrigues, V. Dulic, L. Pichard-Garcia, J.-M. Fabre, P. Blanc, P. Maurel (France)

Human study

........................................................................................................................................................................................................................................................... 178 Profiling the adult human liver transcriptome: analysis by N. Yano, N.A. Habib, K.J. Fadden, H. Yamashita, R. Mitry, cDNA array hybridization Human study

H. Jauregui, A. Kane, M. Endoh, A. Rifai (USA, UK, Japan)

See Editorial, pages 295–296

........................................................................................................................................................................................................................................................... 187 Rat liver slices as a tool to study LPS-induced inflammatory P. Olinga, M.T. Merema, M.H. de Jager, F. Derks, response in the liver

B.N. Melgert, H. Moshage, M.J.H. Slooff, D.K.F. Meijer, K. Poelstra, G.M.M. Groothuis (The Netherlands)

Chronic Liver Disease ...........................................................................................................................................................................................................................................................

195

Findings on liver biopsy to investigate abnormal liver function tests in the absence of diagnostic serology Human study

M.M. Skelly, P.D. James, S.D. Ryder (UK)

See Editorial, pages 290–294

...........................................................................................................................................................................................................................................................

Inflammation and Fibrosis ...........................................................................................................................................................................................................................................................

200

Alpha B-crystallin expression in human and rat hepatic stellate cells

D. Cassiman, T. Roskams, J. van Pelt, L. Libbrecht, P. Aertsen, T. Crabbe´ , H. Vankelecom, C. Denef (Belgium)

Liver Cell Injury and Liver Failure ...........................................................................................................................................................................................................................................................

208

Survival and differentiation of porcine hepatocytes encapsulated by semiautomatic device and allotransplanted in large number without immunosuppression

S. Benoist, R. Sarkis, N. Chafaı¨, V. Barbu, J. Honiger, F. Lakehal, L. Becquemont, M. Baudrimont, J. Capeau, C. Housset, B. Nordlinger (France)

plays a pivotal role in concanavalin A-induced liver injury in mice

K. Tamori, K. Aso, I. Makino (Japan)

........................................................................................................................................................................................................................................................... K. Nakamura, M. Okada, M. Yoneda, S. Takamoto, Y. Nakade, 217 Macrophage inflammatory protein-2 induced by TNF-a

Liver Growth and Cancer ...........................................................................................................................................................................................................................................................

225

Power Doppler signals after percutaneous ethanol injection therapy for hepatocellular carcinoma predict local recurrence of tumors: a prospective study using 199 consecutive patients

S. Sato, Y. Shiratori, M. Imamura, T. Teratani, S. Obi, Y, Koike, Y. Imai, H. Yoshida, S. Shiina, M. Omata (Japan)

Human study

........................................................................................................................................................................................................................................................... 235 Microsatellite instability and alternative genetic pathway in H. Momoi, T. Itoh, Y. Nozaki, Y. Arima, H. Okabe, S. Satoh, intrahepatic cholangiocarcinoma

Y. Toda, E. Sakai, K. Nakagawara, P. Flemming, M. Yamamoto, Y. Shimahara, Y. Yamaoka, M. Fukumoto (Japan, Germany)

Human study

........................................................................................................................................................................................................................................................... T. Torimura, T. Ueno, M. Kin, R. Harada, T. Nakamura, 245 Laminin deposition to type IV collagen enhances haptotaxis, chemokinesis, and adhesion of hepatoma cells through b1integrins

M. Sakamoto, R. Kumashiro, H. Yano, M. Kojiro, M. Sata (Japan)

before surgical resection of hepatocellular carcinoma

V. Vilgrain, B. Terris, V. Moutardier, O. Farges, D. Valla (France)

........................................................................................................................................................................................................................................................... 254 Assessment of the benefits and risks of percutaneous biopsy F. Durand, J.M. Regimbeau, J. Belghiti, A. Sauvanet,

Human study

........................................................................................................................................................................................................................................................... 259 Increased levels of cytosolic thioredoxin reductase activity L. Bjo¨ rkhem, H. Teclebrhan, E. Kesen, J.M. Olsson, and mRNA in rat liver nodules

L.C. Eriksson, M. Bjo¨ rnstedt (Sweden)

Viral Hepatitis ...........................................................................................................................................................................................................................................................

265

Long-term follow-up of chronic hepatitis C in patients diagnosed at a tertiary-care center

X. Forns, S. Ampurdane`s, J.M. Sanchez-Tapias, M. Guilera, M. Sans, A. Sa´ nchez-Fueyo, L. Quinto´ , P. Joya, M. Bruguera, J. Rode´ s (Spain)

Human study

........................................................................................................................................................................................................................................................... L. Alric, M. Duffaut, J. Selves, K. Sandre, M. Mularczyck, 272 Maintenance therapy with gradual reduction of the interferon dose over one year improves histological response in patients with chronic hepatitis C with biochemical response: results of a randomized trial

J. Izopet, H. Desmorat, C. Bureau, N. Chaouche, B. Dalbergue, J.-P. Vinel (France)

Human study

........................................................................................................................................................................................................................................................... 279 Risk factors for diabetes mellitus and early insulin J.-M. Petit, J.-B. Bour, C. Galland-Jos, A. Minello, B. Verges, resistance in chronic hepatitis C

M. Guiguet, J.-M. Brun, P. Hillon (France)

Human study

...........................................................................................................................................................................................................................................................

284

Risk of parenterally transmitted hepatitis following exposure to surgery or other invasive procedures: results from the hepatitis surveillance system in Italy

A. Mele, E. Spada, L. Sagliocca, P. Ragni, M.E. Tosti, G. Gallo, A. Moiraghi, E. Balocchini, M. Sangalli, P.L. Lopalco, T. Stroffolini (Italy)

Human study

Editorials ........................................................................................................................................................................................................................................................... 290

Liver biopsy in elevated liver functions tests? An old question revisited

L. Bianchi (Switzerland)

........................................................................................................................................................................................................................................................... 295 The pros and cons of gene expression analysis by A.K. Groen (The Netherlands) microarrays

Review ........................................................................................................................................................................................................................................................... 297 Oxidative stress-related molecules and liver fibrosis M. Parola, G. Robino (Italy) ...........................................................................................................................................................................................................................................................

Images in Hepatology ........................................................................................................................................................................................................................................................... 307

Expression of liver steatosis in hepatitis C virus infection and pattern of response to a-interferon

L. Rubbia-Brandt, E. Giostra, G. Mentha, R. Quadri, F. Negro (Switzerland)

Letters to the Editor ........................................................................................................................................................................................................................................................... 308

Acute fatal hepatitis related to levofloxacin

L. Spahr, L. Rubbia-Brandt, O. Marinescu, B. Armenian, A. Hadengue (Switzerland)

........................................................................................................................................................................................................................................................... 309 Hepatotoxicity induced by fosinopril M. Romero-Go´ mez, E.J. Miralles, E. Garcı´a Dı´az, A. Robles, E. Sua´ rez, M. Castro (Spain)

........................................................................................................................................................................................................................................................... 310 A life-threatening complication of focal nodular hyperplasia J. Hardwigsen, J. Pons, V. Veit, S. Garcia, Y.P. Le Treut (France)

........................................................................................................................................................................................................................................................... 312 Impact of medical recommendations on alcohol B. Nalpas, S. Martin, H. Fontaine, P. Fabbro-Peray, C. Bre´ chot, consumption in HCV positive patients

S. Pol (France)

Announcements ........................................................................................................................................................................................................................................................... 314

VOL. 35, NO. 3 The Bridge ........................................................................................................................................................................................................................................................... 315

The Bridge – September 2001

J. Reichen

Original Articles Biliary Tract and Cholestasis ...........................................................................................................................................................................................................................................................

316

Bone disease in primary biliary cirrhosis: independent indicators and rate of progression

K.V.N. Menon, P. Angulo, S. Weston, E.R. Dickson, K.D. Lindor (USA)

Human study

........................................................................................................................................................................................................................................................... 324 Apolipoprotein E polymorphism, a marker of disease C. Corpechot, P. Benlian, V. Barbu, O. Chazouille`res, severity in primary biliary cirrhosis? Human study

R.E. Poupon, R. Poupon (France)

See Editorial, pages 412–415

........................................................................................................................................................................................................................................................... 329 Early shifts of adrenal steroid synthesis before and after B. Zietz, I. Wengler, H. Messmann, G. Lock, J. Scho¨ lmerich, relief of short-term cholestasis Human study

See Editorial, pages 416–418

R.H. Straub (Germany)

...........................................................................................................................................................................................................................................................

Chronic Liver Diseases ...........................................................................................................................................................................................................................................................

338

The prognostic value of histology in the assessment of patients with Budd–Chiari syndrome

T.J. Tang, K.P. Batts, P.C. de Groen, B. van Hoek, E.B. Haagsma, W.C.J. Hop, H.L.A. Janssen (The Netherlands, USA)

Human study

........................................................................................................................................................................................................................................................... 344 Venesection therapy of insulin resistance-associated hepatic A. Guillygomarc’h, M.H. Mendler, R. Moirand, F. Laine´ , iron overload

V. Quentin, V. David, P. Brissot, Y. Deugnier (France, USA)

Human study Cirrhosis and its Complications ...........................................................................................................................................................................................................................................................

350

Role of small-conductance Ca 2+-dependent K + channels in in vitro nitric oxide-mediated aortic hyporeactivity to aadrenergic vasoconstriction in rats with cirrhosis

E. Barrie`re, K.A. Tazi, F. Pessione, J. Heller, O. Poirel, D. Lebrec, R. Moreau (France)

genes in rat astrocytes by Northern blotting and differential display reverse transcriptase-polymerase chain reaction

(Germany)

........................................................................................................................................................................................................................................................... U. Warskulat, S. Kreuels, H.W. Mu¨ ller, D. Ha¨ ussinger 358 Identification of osmosensitive and ammonia-regulated

Inflamation and Fibrosis ...........................................................................................................................................................................................................................................................

367

Transglutaminase-mediated cross-linking is involved in the stabilization of extracellular matrix in human liver fibrosis

P. Grenard, S. Bresson-Hadni, S. El Alaoui, M. Chevallier, D.A. Vuitton, S. Ricard-Blum (France)

Human study

........................................................................................................................................................................................................................................................... 376 Effect of angiotensin II type 1 receptor blockade on G. Paizis, R.E. Gilbert, M.E. Cooper, P. Murthi, J.M. Schembri, experimental hepatic fibrogenesis

L.L. Wu, J.R. Rumble, D.J. Kelly, C. Tikellis, A. Cox, R.A. Smallwood, P.W. Angus (Australia)

........................................................................................................................................................................................................................................................... 386 Modulation of collagen XVIII/endostatin expression in J.-D. Jia, M. Bauer, N. Sedlaczek, H. Herbst, M. Ruehl, lobular and biliary rat liver fibrogenesis

E.G. Hahn, E.O. Riecken, D. Schuppan (Germany)

fibrosis is mediated by downregulation of procollagen a1(I) and TIMP-1

E.O. Riecken, D. Schuppan (Germany, Italy)

........................................................................................................................................................................................................................................................... J.-D. Jia, M. Bauer, J.J. Cho, M. Ruehl, S. Milani, G. Boigk, 392 Antifibrotic effect of silymarin in rat secondary biliary

Viral Hepatitis ...........................................................................................................................................................................................................................................................

399

Quantitation of hepatic hepatitis C virus RNA in patients with chronic hepatitis C. Relationship with severity of disease, viral genotype and response to treatment

A. Gervais, M. Martinot, N. Boyer, A. Auperin, V. Le Breton, C. Degott, D. Valla, P. Marcellin (France)

Human study

........................................................................................................................................................................................................................................................... G. Barbaro, F. Zechini, A.M. Pellicelli, R. Francavilla, 406 Long-term efficacy of interferon alpha-2b and lamivudine in combination compared to lamivudine monotherapy in patients with chronic hepatitis B. An Italian multicenter, randomized trial Human study

G. Scotto, D. Bacca, M. Bruno, S. Babudieri, M. Annese, F. Matarazzo, G. Di Stefano, G. Barbarini, for the Lamivudine Italian Study Group Investigators (Italy)

See Editorial, pages 419–420

Editorials ........................................................................................................................................................................................................................................................... 412

The natural history of primary biliary cirrhosis: of genes and cooperation

M.E. Gershwin (USA)

........................................................................................................................................................................................................................................................... 416 Cytokines and neuroendocrine dysregulation in obstructive M.G. Swain (Canada) cholestasis: pathophysiological implications

........................................................................................................................................................................................................................................................... 419 Lamivudine-interferon combination therapy for chronic S.W. Schalm (The Netherlands) hepatitis B: further support but no conclusive evidence

Special Article ........................................................................................................................................................................................................................................................... 421

Clinical Management of Hepatocellular Carcinoma. Conclusions of the Barcelona-2000 EASL Conference

J. Bruix, M. Sherman, J.M. Llovet, M. Beaugrand, R. Lencioni, A.K. Burroughs, E. Christensen, L. Pagliaro, M. Colombo, J. Rode´ s, for the EASL Panel of Experts on HCC

Images in Hepatology ........................................................................................................................................................................................................................................................... 431

Abnormal hepatic enhancement on CT in congestive heart failure

D. de las Heras, M. Pages (Spain)

Letters to the Editor ........................................................................................................................................................................................................................................................... 432

Steatosis in chronic hepatitis C: can weight reduction improve therapeutic efficacy?

E. Giannini, P. Ceppa, R. Testa (Italy)

........................................................................................................................................................................................................................................................... 433 Reply A.D. Clouston, I.J. Hickman, G.A. Macdonald, J.R. Jonsson, E.E. Powell (Australia)

VOL. 35, NO. 4 The Bridge ........................................................................................................................................................................................................................................................... 435

The Bridge – October 2001

J. Reichen

Original Articles Biliary Tract and Cholestasis ...........................................................................................................................................................................................................................................................

436

FIC1, the protein affected in two forms of hereditary cholestasis, is localized in the cholangiocyte and the canalicular membrane of the hepatocyte Human study

E.F. Eppens, S.W.C. van Mil, J.M.L. de Vree, K.S. Mok, J.A. Juijn, R.P.J. Oude Elferink, R. Berger, R.H.J. Houwen, L.W.J. Klomp (The Netherlands)

See Editorial, pages 522–524

........................................................................................................................................................................................................................................................... K.J. van Erpecum, D.Q.-H. Wang, F. Lammert, B. Paigen, 444 Phenotypic characterization of Lith genes that determine susceptibility to cholesterol cholelithiasis in inbred mice: soluble pronucleating proteins in gallbladder and hepatic biles

A.K. Groen, M.C. Carey (USA, The Netherlands)

See Editorial, pages 525–526 Cirrhosis and its Complications ...........................................................................................................................................................................................................................................................

452

Nitric oxide synthase activity in the splanchnic vasculature of patients with cirrhosis: relationship with hemodynamic disturbances

L. Albornoz, A. Motta, D. Alvarez, A. Estevez, J.C. Bandi, L. McCormack, J. Matera, C. Bonofiglio, M. Ciardullo, E. de Santiban˜ es, M. Gimeno, A. Gadano (Argentina)

Human study Inflammation and Fibrosis ...........................................................................................................................................................................................................................................................

457

Prevention of hepatic cirrhosis in rats by hydroxyl radical scavengers

R. Bruck, H. Shirin, H. Aeed, Z. Matas, A. Hochman, M. Pines, Y. Avni (Israel)

MT1-MMP mRNA in the recovery from liver fibrosis in the rat

K. Maruyama, A. Okada, I. Okazaki (Japan)

progression of rat liver fibrosis in association with inactivation of hepatic stellate cells

H. Furuyama, K. Furumoto, T. Nakao, N. Isobe, M. Imamura (Japan)

........................................................................................................................................................................................................................................................... 465 Dynamic change of cells expressing MMP-2 mRNA and T. Watanabe, M. Niioka, A. Ishikawa, S. Hozawa, M. Arai, ........................................................................................................................................................................................................................................................... T. Murata, S. Arii, T. Nakamura, A. Mori, T. Kaido, 474 Inhibitory effect of Y-27632, a ROCK inhibitor, on ...........................................................................................................................................................................................................................................................

Liver Cell Injury and Liver Failure ...........................................................................................................................................................................................................................................................

482

Agmatine induces apoptosis in rat hepatocyte cultures

G. Gardini, C. Cabella, C. Cravanzola, C. Vargiu, S. Belliardo, G. Testore, S.P. Solinas, A. Toninello, M.A. Grillo, S. Colombatto (Italy)

........................................................................................................................................................................................................................................................... B. Wielockx, F. Bussolino, S.D. Shapiro, C. Libert (Belgium, 490 Involvement of a serine protease, but not of neutrophil elastase, in tumor necrosis factor-induced lethal hepatitis and induction of platelet-activating factor

Italy, USA)

Liver Growth and Cancer ...........................................................................................................................................................................................................................................................

498

Liver cancer risk is increased in patients with porphyria cutanea tarda in comparison to matched control patients with chronic liver disease

A.L. Fracanzani, E. Taioli, M. Sampietro, E. Fatta, C. Bertelli, G. Fiorelli, S. Fargion (Italy)

Human study

........................................................................................................................................................................................................................................................... 504 Cloning and characterization of the 5 0 -flanking region of M. Kagaya, S. Kaneko, H. Ohno, K. Inamura, K. Kobayashi human cytokeratin 19 gene in human cholangiocarcinoma cell line

(Japan)

Viral Hepatitis

........................................................................................................................................................................................................................................................... 512 Interferon plus amantadine versus interferon alone in the S. Caronia, M.F. Bassendine, R. Barry, P. Mills, treatment of naı¨ve patients with chronic hepatitis C: a UK multicentre study

Human study

N.V. Naoumov, R. Fox, J. Lowes, D. Hollanders, I. MurrayLyon, W.L. Irving, R.D. Goldin, G.R. Foster, on behalf of the UK Amantadine Study Group (UK)

See Editorial, pages 527–530

........................................................................................................................................................................................................................................................... 517 Interferon and amantadine in combination as initial M. Tabone, C. Laudi, B. Delmastro, A. Biglino, M. Andreoni, treatment for chronic hepatitis C patients

Human study

F. Chieppa, R. Bonardi, G. Cariti, S. Cusumano, F. Brunello, G. Calleri, A. Manca, P. Della Monica, L. Sidoli, M. Rizzetto, A. Pera (Italy)

See Editorial, pages 527–530

Editorials

........................................................................................................................................................................................................................................................... 522 FIC1: another bile salt carrier within the enterohepatic B. Stieger (Switzerland) circulation?

........................................................................................................................................................................................................................................................... 525 Pronucleating proteins in bile – a myth? S.P. Lee, C.W. Ko (USA) ........................................................................................................................................................................................................................................................... 527 Amantadine for chronic hepatitis C: a magic bullet or yet A. Craxı`, O. Lo Iacono (Italy) another dead duck?

Special Article ........................................................................................................................................................................................................................................................... 531 The spectrum of liver disease in the general population: S. Bellentani, C. Tiribelli (Italy) lesson from the Dionysos study

Images in Hepatology ........................................................................................................................................................................................................................................................... 538 Liver glycogen storage associated with uncontrolled type 1 M. Torres, D. Lo´ pez (Spain) diabetes mellitus

Letters to the Editor ........................................................................................................................................................................................................................................................... 539 Zafirlukast-related hepatitis: report of a further case G.C. Actis, A. Morgando, M. Lagget, E. David, M. Rizzetto (Italy)

........................................................................................................................................................................................................................................................... 541 Acute hepatocellular injury associated with zafirlukast J.R. Mole´ s, J. Primo, J.M. Ferna´ ndez, J.E. Hinojosa (Spain) ........................................................................................................................................................................................................................................................... 542 Successful liver transplantation for diffuse biliary J.V. Dumortier, J.-Y. Scoazec, P.-J. Valette, T. Ponchon, papillomatosis

O. Boillot (France)

...........................................................................................................................................................................................................................................................

543

Randomized pilot trial of vitamin K2 for bone loss in patients with primary biliary cirrhosis

S. Nishiguchi, S. Shimoi, H. Kurooka, A. Tamori, D. Habu, T. Takeda, S. Kubo (Japan)

...........................................................................................................................................................................................................................................................

Book Review ........................................................................................................................................................................................................................................................... 546 The Hepatocyte Review R. Hoekstra

Announcements ........................................................................................................................................................................................................................................................... 547

VOL. 35, NO. 5 The Bridge ........................................................................................................................................................................................................................................................... 549

The Bridge – November 2001

J. Reichen

Original Articles Biliary Tract and Cholestasis ...........................................................................................................................................................................................................................................................

550

Insulin injections enhance cholesterol gallstone incidence by changing the biliary cholesterol saturation index and apo A-I concentration in hamsters fed a lithogenic diet

S. Dubrac, M. Parquet, Y. Blouquit, D. Gripois, M.-F. Blouquit, M. Souidi, C. Lutton (France)

Cell Biology, Metabolism and Transport ...........................................................................................................................................................................................................................................................

558

Regulation of insulin-like growth factor-I and of insulin-like growth factor binding protein-1, -3 and -4 in cocultures of rat hepatocytes and Kupffer cells by interleukin-6

A. Lelbach, J.-G. Scharf, G. Ramadori (Germany)

Chronic Liver Diseases ...........................................................................................................................................................................................................................................................

568

Non-alcoholic fatty liver disease in an area of southern Italy: main clinical, histological, and pathophysiological aspects

C. Loguercio, V. De Girolamo, I. de Sio, C. Tuccillo, A. Ascione, F. Baldi, G. Budillon, L. Cimino, A. Di Carlo, M.P. Di Marino, F. Morisco, F. Picciotto, L. Terracciano, R. Vecchione, V. Verde, C. Del Vecchio Blanco (Italy)

Human Study

........................................................................................................................................................................................................................................................... 575 High prevalence of the H1069Q mutation in East German K. Caca, P. Ferenci, H.-J. Ku¨ hn, C. Polli, H. Willgerodt, patients with Wilson disease: rapid detection of mutations by limited sequencing and phenotype–genotype analysis

B. Kunath, W. Hermann, J. Mo¨ ssner, F. Berr (Germany, Austria)

Human Study

........................................................................................................................................................................................................................................................... L. Spahr, L. Rubbia-Brandt, J. Pugin, E. Giostra, J.-L. Frossard, 582 Rapid changes in alcoholic hepatitis histology under steroids: correlation with soluble intercellular adhesion molecule-1 in hepatic venous blood

B. Borisch, A. Hadengue (Switzerland)

Human Study Cirrhosis and its Complications ...........................................................................................................................................................................................................................................................

590

Salvage transjugular intrahepatic portosystemic shunt for uncontrolled variceal bleeding in patients with decompensated cirrhosis Human Study

D. Azoulay, D. Castaing, P. Majno, F. Saliba, P. Ichaı¨, A. Smail, V. Delvart, M. Danaoui, D. Samuel, H. Bismuth (France)

See Editorial, pages 658–660

........................................................................................................................................................................................................................................................... 598 The development of low-grade cerebral edema in cirrhosis J. Co´ rdoba, J. Alonso, A. Rovira, C. Jacas, F. Sanpedro, is supported by the evolution of 1H-magnetic resonance abnormalities after liver transplantation Human Study

See Editorial, pages 661–662

L. Castells, V. Vargas, C. Margarit, J. Kulisewsky, R. Esteban, J. Guardia (Spain)

...........................................................................................................................................................................................................................................................

605

Blood volume distribution in patients with cirrhosis: aspects of the dual-head gamma-camera technique

M. Kiszka-Kanowitz, J.H. Henriksen, S. Møller, F. Bendtsen (Denmark)

Human Study Liver Cell Injury and Liver Failure ...........................................................................................................................................................................................................................................................

613

In situ detection of oxidative DNA damage, 8-hydroxydeoxyguanosine, in chronic human liver disease

T. Kitada, S. Seki, S. Iwai, T. Yamada, H. Sakaguchi, K. Wakasa (Japan)

Human Study

........................................................................................................................................................................................................................................................... 619 Suppression of tumor necrosis factor-a production and K. Yonezawa, Y. Yamamoto, H. Yamamoto, Y. Ishikawa, neutrophil infiltration during ischemia–reperfusion injury of the liver after heat shock preconditioning

H. Uchinami, K. Taura, A. Nakajima, Y. Yamaoka (Japan)

See Editorial, pages 663–665

........................................................................................................................................................................................................................................................... 628 Toxicity of amiodarone and amiodarone analogues on M. Spaniol, R. Bracher, H.R. Ha, F. Follath, S. Kra¨ henbu¨ hl isolated rat liver mitochondria

(Switzerland)

Liver Growth and Cancer ...........................................................................................................................................................................................................................................................

637

Overexpression of tetraspanin CO-029 in hepatocellular carcinoma

K. Kanetaka, M. Sakamoto, Y. Yamamoto, S. Yamasaki, F. Lanza, T. Kanematsu, S. Hirohashi (Japan, France)

Human Study

........................................................................................................................................................................................................................................................... 643 Transmembrane carbonic anhydrase, MN/CA IX, is a J. Saarnio, S. Parkkila, A.-K. Parkkila, S. Pastorekova´ , potential biomarker for biliary tumours

K. Haukipuro, J. Pastorek, T. Juvonen, T.J. Karttunen (Finland, Slovak Republic)

Human Study Viral Hepatitis ...........................................................................................................................................................................................................................................................

650

Natural cytotoxicity and antibody-dependent cellular cytotoxicity (ADCC) is not impaired in patients suffering from chronic hepatitis C

U. Du¨ esberg, A.M. Schneiders, D. Flieger, G. Inchauspe´ , T. Sauerbruch, U. Spengler (Germany, France)

Human Study

Editorials ........................................................................................................................................................................................................................................................... 658

Salvage transjugular intrahepatic portosystemic shunt: is it really life-saving?

J. Bosch (Spain)

........................................................................................................................................................................................................................................................... 661 Images of the brain in chronic liver failure R.F. Butterworth (Canada) ........................................................................................................................................................................................................................................................... 663 Heat shock: a barrier to hepatic ischemic injury J.M. Mato, F.J. Corrales, M.A. Avila (Spain)

Review ........................................................................................................................................................................................................................................................... 666

Hepatitis C and liver transplantation

M. Berenguer, F.X. Lo´ pez-Labrador, T.L. Wright (Spain, USA)

Case Report ........................................................................................................................................................................................................................................................... 679

Favorable outcome of orthotopic liver transplantation in a patient with subacute liver failure due to the emergence of a hepatitis B YMDD escape mutant virus

P. Sta¨ rkel, Y. Horsmans, A. Geubel, O. Ciccarelli, P. Goubau, J. Rahier, J. Lerut (Belgium)

Images in Hepatology ........................................................................................................................................................................................................................................................... 682

Transjugular intrahepatic splenosystemic shunt in a patient with portal vein thrombosis

F. Trevisani, C. Rossi, F. Losinno, M. Bernardi (Italy)

...........................................................................................................................................................................................................................................................

Letters to the Editor ........................................................................................................................................................................................................................................................... 683

L. Fartoux-Heymann, C. He´ zode, E.S. Zafrani, D. Dhumeaux, A. Mallat (France)

Acute fatal hepatitis related to sertraline

........................................................................................................................................................................................................................................................... 684 The low incidence of primary liver disease in patients with M. Basaranoglu, A. Sonsuz, H. Senturk, P. Akin (Turkey) nonalcoholic steatohepatitis

........................................................................................................................................................................................................................................................... 685 Reply C. Garcı´a-Monzo´ n, R. Moreno-Otero (Spain) ........................................................................................................................................................................................................................................................... 685 LKM1 antibody and interferon therapy in children with R. Iorio, A. Giannattasio, G. Vespere, A. Vegnente (Italy) chronic hepatitis C

........................................................................................................................................................................................................................................................... O. Ceci, M. Margiotta, F. Marello, R. Francavilla, E. Lerardi, 687 High rate of spontaneous viral clearance in a cohort of vertically infected hepatitis C virus infants: what lies behind?

P. Loizzi, L. Impedovo, A. Francavilla (Italy)

Book Review ........................................................................................................................................................................................................................................................... 689

Portal Hypertension III. Proceedings of the Third Baveno International Concensus Workshop on Definitions, Methodology and Therapeutic Strategies

J. Bosch

Announcements ........................................................................................................................................................................................................................................................... 690

VOL. 35, NO. 6 The Bridge ........................................................................................................................................................................................................................................................... 691

The Bridge – December 2001

J. Reichen

Original Articles Cell Biology, Metabolism and Transport ...........................................................................................................................................................................................................................................................

692

S-Adenosylmethionine modulates inducible nitric oxide synthase gene expression in rat liver and isolated hepatocytes

P.L. Majano, C. Garcı´a-Monzo´ n, E.R. Garcı´a-Trevijano, F.J. Corrales, J. Ca´ mara, P. Ortiz, J.M. Mato, M.A. Avila, R. Moreno-Otero (Spain)

counteracts established steroid catabolism in rats by effects on hepatic amino-N degradation

(Denmark)

........................................................................................................................................................................................................................................................... T. Grøfte, D.S. Jensen, J. Greisen, N. Tygstrup, H. Vilstrup 700 Growth hormone and insulin-like growth factor-I

Chronic Liver Diseases ...........................................................................................................................................................................................................................................................

707

Haptoglobin phenotype 2-2 overrepresentation in Cys282Tyr hemochromatotic patients

H. Van Vlierberghe, M. Langlois, J. Delanghe, Y. Horsmans, P. Michielsen, J. Henrion, R. Cartuyvels, J. Billiet, M. De Vos, G. Leroux-Roels (Belgium)

Human study Cirrhosis and its Complications ...........................................................................................................................................................................................................................................................

712

Multicenter randomized controlled trial comparing different schedules of somatostatin in the treatment of acute variceal bleeding

E. Moitinho, R. Planas, R. Ban˜ ares, A. Albillos, L. Ruiz-delArbol, C. Ga´ lvez, J. Bosch, Variceal Bleeding Study Group (Spain)

Human study

........................................................................................................................................................................................................................................................... W.-H. Kim, H. Park, C. Yun, H. Cho, S. Kim, W.K. Paik, 719 Mixture of N-carbamoyl-l-glutamate plus l-arginine can protect rats with liver cirrhosis from acute ammonia intoxication

S.-H. Jeon, J.-H. Lee (South Korea)

...........................................................................................................................................................................................................................................................

726

Worsening of hepatic dysfunction as a consequence of repeated hydroxyethylstarch infusions

C. Christidis, F. Mal, J. Ramos, A. Senejoux, P. Callard, R. Navarro, J.-C. Trinchet, D. Larrey, M. Beaugrand, C. Guettier (France)

Human study

........................................................................................................................................................................................................................................................... 733 Prolonged QTc interval and its relationship to autonomic L. Puthumana, V. Chaudhry, P.J. Thuluvath (USA) cardiovascular reflexes in patients with cirrhosis Human study Inflammation and Fibrosis ...........................................................................................................................................................................................................................................................

739

Human myofibroblastic hepatic stellate cells express Ca 2+activated K + channels that modulate the effects of endothelin-1 and nitric oxide

X. Gasull, R. Bataller, P. Gine`s, P. Sancho-Bru, J.M. Nicola´ s, M.N. Go¨ rbig, E. Ferrer, E. Badı´a, A. Gual, V. Arroyo, J. Rode´ s (Spain)

Decreasing fibrogenesis: an immunohistochemical study of paired liver biopsies following lamivudine therapy for chronic hepatitis B

Y.-O. Kweon, Z.D. Goodman, J.L. Dienstag, E.R. Schiff, N.A. Brown, E. Burkhardt, R. Schoonhoven, D.A. Brenner, M.W. Fried (USA, Germany)

........................................................................................................................................................................................................................................................... 749

Human study Liver Cell Injury and Liver Failure ...........................................................................................................................................................................................................................................................

756

Mechanism of azathioprine-induced injury to hepatocytes: roles of glutathione depletion and mitochondrial injury

A.U. Lee, G.C. Farrell (Australia)

Viral Hepatitis ...........................................................................................................................................................................................................................................................

765

Decreased expression of an ATP-binding cassette transporter, MRP2, in human livers with hepatitis C virus infection Human study

E. Hinoshita, K.-i. Taguchi, A. Inokuchi, T. Uchiumi, N. Kinukawa, M. Shimada, M. Tsuneyoshi, K. Sugimachi, M. Kuwano (Japan)

See Editorial, pages 811–813

........................................................................................................................................................................................................................................................... 774 Enhanced peripheral T-cell apoptosis in chronic hepatitis C E. Toubi, A. Kessel, L. Goldstein, G. Slobodin, E. Sabo, virus infection: association with liver disease severity Human study

Z. Shmuel, E. Zuckerman (Israel)

See Editorial, pages 814–815

........................................................................................................................................................................................................................................................... 781 Plasminogen deficiency results in poor clearance of nonV.L. Ng, G.E. Sabla, H. Melin-Aldana, N. Kelley-Loughnane, fibrin matrix and persistent activation of hepatic stellate cells after an acute injury

J.L. Degen, J.A. Bezerra (USA)

human hepatocytes

R. Williams, N.V. Naoumov (UK, Switzerland)

infection in chronic hepatitis B patients

K. Mochizuki, A. Kaneko, K. Yamamoto, K. Hikiji, M. Kato (Japan)

........................................................................................................................................................................................................................................................... 790 Non-cytolytic inhibition of hepatitis B virus replication in D. Suri, R. Schilling, A.R. Lopes, I. Mullerova, G. Colucci, ........................................................................................................................................................................................................................................................... 798 Long-term clinical impact of occult hepatitis B virus M. Komori, N. Yuki, T. Nagaoka, M. Yamashiro,

Human study

........................................................................................................................................................................................................................................................... 805 Lamivudine and alpha-interferon in combination long term I. Tatulli, R. Francavilla, G.L. Rizzo, V. Vinciguerra, E. Ierardi, for precore mutant chronic hepatitis B

A. Amoruso, C. Panella, A. Francavilla (Italy)

Human study

Editorials ........................................................................................................................................................................................................................................................... 811

Why are patients with liver disease jaundiced? ATP-binding cassette transporter expression in human liver disease

P.L.M. Jansen, T. Roskams (The Netherlands, Belgium)

........................................................................................................................................................................................................................................................... 814 A new route to apoptosis in hepatitis C virus infection L. Muratori, D. Gibellini (Italy)

Review ........................................................................................................................................................................................................................................................... 816

Hepatic steatosis and very low density lipoprotein secretion: the involvement of apolipoprotein E

A.R. Mensenkamp, L.M. Havekes, J.A. Romijn, F. Kuipers (The Netherlands)

Case Report ........................................................................................................................................................................................................................................................... 823

Lamivudine treatment failure in preventing fatal outcome of de novo severe acute hepatitis B in patients with haematological diseases

E. Petrelli, M. Balducci, C. Pieretti, M.B.L. Rocchi, M. Clementi, A. Manzin (Italy)

Images in Hepatology ........................................................................................................................................................................................................................................................... 827

Focal intrahepatic storage of oligomannosides mimicking a primitive liver neoplasm

F. Brunello, N. Leone, L. Delsedime, M. Gaido, P. Carucci, M. Rizzetto (Italy)

...........................................................................................................................................................................................................................................................

Letters to the Editor ........................................................................................................................................................................................................................................................... 828

Long term survival from progressive multifocal leucoencephalopathy after liver transplantation

J.R. Boulton-Jones, C. Fraser-Moodie, S.D. Ryder (UK)

virus infection

K. Takase, T. Nakano, Y. Tameda (Japan)

........................................................................................................................................................................................................................................................... 829 Hepatitis B genotypes in patients with acute hepatitis B T. Sakai, K. Shiraki, K. Sugimoto, S. Ohmori, K. Murata, ........................................................................................................................................................................................................................................................... 830 Acute hepatitis with extremely high ferritin levels and Still’s N. Assy, S. Schlesinger, O. Hussein (Israel) disease

List of Referees ........................................................................................................................................................................................................................................................... 832

Announcements ........................................................................................................................................................................................................................................................... 835

Index ...........................................................................................................................................................................................................................................................