024 Expression of the Fas and Fas ligand in cutaneous lupus

JSID Abstracts

019

022

HSP27,90 SEVERE

INDUCIIGN

BURN

Depanmentof

study.

‘Ibb

otgam

the induetmn

tn systemic

OF RA?S

organs

develqkadontheraS

that s-

bum

at the molexlar

of unother

leveL

r$pes of

for3 sec. AfeerO, 24 aed48hrs

of bum trtjwy.

Wesernblctanalysis.

was examinedby

pninent.

tbe induction

These reds systemic

the induction

bttiure

tha

rat”

HSP27,R

bun

sod IL13 in ptlents a rmloglc

by enzyme-Ihhd

significantly

HSP72 WRBS

bununosorbent

higher in both ISSc and dSSe ptients

10 were signitieantly

tlw

war

of IL-4 ad IL13 were

in controls. Setum levels of IL

higher only in dSSc patients than in controls. Serum levels of IL.

13 were correlated with qthmeyte

in

assay. Serum kvek

sedbnentation rate rind C-reactive protein levels in

in all

patients. We suggest that IL4, ILlO,

most

contribute to the disease proew, sod that IL13 msy be rr serologic lndieatot of systemic intlnmmation in patients with SSc.

WPT bwest

of HSP27,W

w6

and 90 wee

diffmntly

and IL13 may be among the cytobines that

organs after severe bum injury

023

EFFECT

OF AGING

ON THE INDUCTION

OF STRESS PROTEIN

HUMAN SKIN.T.Muramatsu*.M.Hatoko.H.Tada.S.Flirai Department

of Dermatology.

Nara Medical

NORMAL

IN

and T.Shirai. University.

Nara. Japan. In order

to know the effect

on the induction human

skin.

the

organ-cultured

of chronological

of heat shock protein 7-m

HSP (HSP72)

aging

(HSP) in normal

was

examintxl

in

normal human skin which were obtained

from 30 individuals (age range:17-R6 years). lmmunostaining using a monoclonal antlbody specific for the HSP72 revealed that although the time course of the heat-induced HSP72 expression was similar in the young and aged group. lower level of induction of HSP72 was observed in aged group. This result

indicates

dysfunction

of the heat shock

that

there

is an age-related in normal

response

IDENTLFICATION OF RANTES AND GM-CSF AS EOSINOPHIL A’l-l’RACTANTS PRODUCED BY STIMULATED DERMAL FIBROBLASTS. N. Noso”~‘.S. ‘Depanmmt of Demtatology, Yamarnold. E. Chrktophers’, Jerk-M. Schttide?. Hiroshima Unhersity School nf Medkine, Hiroshima, Japan.‘Department of Dermatokrgy, University af Kiel, Kiel, Ormany. WC addroned the question whether Sbroblasts, the major demtal cellular wnstituenu release ensinnphil (Eo) chemntaaic cytokines. Supematants of cultivated demtal Ebmblasts were tested fnr Eo-chemotaetic aclivity using purified human Ens and the Boyden chamber system. As a result, TNFa and IL1 huhteed production of Fo-attrading activity. For further binchemical charncterization supentatrots were sepanited by hepdn-sephaose-HFLC. Stmng El,-chemotactic activity war; fnund to bind to the column, whereas high tuttonne of activit) also dd not bind to heparin. Heparin-bound Eo-chemotactic material was puritied to homogoteny h:: btferent Hl’LC steps resulting in a XkD pnlypepthb. Aminotemtinal aninn add scplence analysis revealed identity with that of the chemokine RANTES missing twn aminotenninaJ r&&s. The other, non-hepadn hinting pe& of Eochcmotactic actvity, wu brther purified and could be identifiied as GM-CSF by inhibition with a neutralizmg antibody as well as ELlSA In conclusion, dmnal tibroblasts seuete upon TNFa- and IL-l-stimulation the cytokines RANTFS wd GM-CSF as major Eoattrdding fixtan and thus muld be important fiu the recmitment of Ens in the lesion of atopic dermalitis.

skin.

human

024

021 REGULATION

OF ELASTASE

Moriwaki*. Research, The in skin elasticity. dermal coding elastase. cytokines

N.

contrast.

Morisaki.

Kao Corp. biochemical is

indicate

by IL-4 that

in

Cl.

EXPRESSION

Imokawa.

BY CYTOKINES.

lnstitutc

Tochigi. Japan. mechanisms involved

not well understood In the course of

>5

for in

s.

Fundamental

clilstin

despite its essential cloning elaslasc cDNA

fold

ns compared

markedly

suppressed

premyelocitic

leukocyte, between

GENE

metabolism role in skin from human

fibroblasts(HFB), we found that HFB expresses a gene elastasc (EL) which is identical to that of a leukocyte RT-PCR analysis of EL mRNA in HFB treated with various revealed that IL-4 remarkably augmented EL gene

expression

IL-4

ILlO,

(n=45), diffu.% culanmus ssc (dssc) patients (n=zs), ad mnboJ subjeds (“do) examhd

020

a

(IL-Q

(&Se) are elevated ;nd w eon&ted with the dhtiul

festwes of this dlseaw. Serum samples from limited euwreotts SSc (lS.Sc) ptiene

(9O’C)

d HSP27,90

rate of HSPR

with systemic r&rosls

protein

hf.

Universily Fueulty of

of Tokyo, Tokyo, Ispan.

To detentdue whether serum levels of btierleukin4

HSP27,Wof

Thelevelof

gland,

inducedin

shock

tbe expressionof

but there was no change of the kvd

Medicine. University

dfeet

tbehotwater

hypophyah.

In contrast,

Fujimotoz, ad K. Kikuchie. ttkpmtment of Dexm~tology, w SehOOl of Medicine, Kfamva, Japan. QpmIment of Demtobgy.

In the prosent

hea

highest in hypophysh

aranined.

SERUM LEVEU OF INIERLBUKlN-t, lNTBRLEUKU+lO. AND INTBRlBUKlN13 lN PATTENTS WDH SYSTEMIC SCLEROSIS. M. Haqaw*t, K T&eimml,

induced

of the severe@ burned tats. FuIl-thieknes

variousorgans

argans

injtxy

tha severe burn ittjuy

s!einbyrmmetsingtherrsinm

I” a&em”

AFTW

Univetuity,Nanr,Japan.

result suggesa

also systenuc

we examined

HSP27,90

Medical

we have qotted

vegans.

not only skin bu

ORGANS

S.Hirpi’.

Demuuology.Nera

in oystentc

study,

IN SYSTEMIC

INJURY.

In Ihe previous HSPR

ws

185

the the

lymphoma

although regulatory two

cell

a non-treated expression

line,

HL-60.

control.

of

EL

These

gene

and of

suggest

elastin

physiological

metabolism

in

involvement the

dermis.

By in

findings

HFB exprcsscs EL gene identical to mcchamisms of the gene expression

cells

regulation

with the

that of differ of

EXPRESSION OF THE FAS AND FAS LIGAND IN CUTANEOUS LUPUS. M.Nakaiimal. A. Nakaiima2. N. Kavaeaki2. K. Okumura2. M. ‘Department of Dermatology, Nippon Medical School, Tokyo. Honda]. ‘Department of Immunology, Juntendo University, School of Medicirte,Tokyo, Japan. Ligand of Fas (FasL) is a type II membrane protein which belongs the tumor necrosis factor family. Ligation of FasL to its counterreceptor Fas induces apoptosis of Fas-expressing cells. However. the in viva relevance of these molecules for cutaneous immunity is presently unclear. In the present study, we investigate the contribution of Fas-FasL interaction in the pathogenesis of cutaneous lupus by immunohistichemical methods using a panel of mAbs directed against FarL. Fas, CD4, CDS. CD20. KiM6, and CDIa. Fas antigen was expressed on the epidermal cells, hair follicles, and on intiItrating cells. In contrast, expression of FasL was limited on intiltrating cells, especiaIIy around appendages such as hair follicles. These results might reflect tissue destruction by Fas-FasL interactions. However, in lesions of cutaneous vasuculitls and demtatomyositis, expression of FasL was limited only on intiltrating cells around vasctdatures. indicating that Fas-FasL interaction plays a critical role in the destruction of appendages which is a characteristic feature of c”taneo”s lupus.