028 Differentiation between host immune response to candida antigens in localized superficial candidiasis and chronic mucocutaneous candidiasis patients

JSID Abstracts

025

028

HYPERGAMMAGLOBULINEMIC PURPURA OF WALDENSTROM AND RO/SSA AUTOANTIBODIES. 9. Mivapma”. T. Fboto. and T. Shirai. Department of Dermatology, Nara Medical University, Nara, Japan Seven out of nine patients (78%) with hypergammaglobulinemic purpura of WaldenstrBm (HGP) were found to have antibodies to Ro/SSA. In all of the seven patients, associated diseases were recognized in 2-15 years. Five had primary SjUgren’s syndrome, one had rheumatoid arthritis, and one had systemic lupus erythematosus with Sjirgren’s syndrome and thyroid&is. Our study suggests that screening for antibodies to Ro/SSA, especially to the 52kd component of Ro/SSA, is important in the diagnosis of patients with HGP, and might be helpful in predicting prognosis.

DIFFERENTL4TION BEIWEEN HOST IMMUNE RESPONSETOCANDIDA ANTIGENS W LOCALlZED SUPERFlClAL CANDIDIASIS AND CHRONIC MUCOXiTANBJUS CANDIDIASIS PATIENTS. T. M. Masuzawa. and K. Katsuoka. Department of Dermatology. of Medicine Kzmagawa.1apae

Kitasato University

School

The dlavedskin reactionto candidaantiem waseositivc in Ibe heelthvsubiectssod thelesionsbt the localize c&&&s patients.and&e PBMC from these&e&s pnxluce bigb-levelsof IFN-y in responseto samulatieewith candidaantigen.Theseresultswere not observed d cdl in CMCC. but a high-levelof IL-10 pmdectienwss&wed The pcripherelbleedT cellsfmm thehealthysub@& andthelecalizedcendidiesispatients diffccmtiateinto IFN-7 predu&ngcells upmuin vitro stimulation with candida antigen. But such differentiation

was not observed in T cells from the CMCC patienW. Tbe

penphemlblmd T c&s firm the CMCC padentsdifferentiatemte lL IOproducingeells upm in vitro stimulation with candida aatigen. These findings suggest that Ihe establishment of a candida-antigen-spenfic DTH reaction inhibited lesion expamim

in

cutettmw ddissis. The msm why car&b aetigsn-specificDTH reactim is not inducedin CMCC patientsis suspectedto residein theinsufticient antigen-spccitic IFN-y productionandtheoverexprcssio40tIL-IO.

026

029

TREATMENT WITH LIP0 PGE I PREPARATIONS IN PATIENTS WITH DIFFUSE CONNECTIVB TISSUE DISEASE AND PATlEN’SS WITH NEURALGIA -EVALUATION BY ACCELERATION PULSE WAVE. T.Okuda, Y.Umezawa. M.Hirata. T.Dh-i and M. Kona. Department of Dermatology, Tokyo Medical College, Tokyo, JAPAN. Lipo PGEl preparations have shown to be e&etive for the treatment of skin manifestations associated with diffuse cona&ive tissue disease in several studies. There are, however, oo objective aiterla for evaluating the efficacy of the drug. We investigated whether the efficacy could be asxased by ecceleretion pulse wave. we studies 10 patients tith @#use conne&tive tissue disease (4 SLE, 4 PSS and 2 detmatomycaltis) and 10 patients with neuralgia subsquent to herpes wster es contml. Accclcretion pulse wave was measured at the sclerodaetyly or cold spat of the finger tip ia patients with diffuse comt&ive tissue disease and at the finger tip oppo& the a&&ted part in patients with neuralgia, before and after 1Oug of Lipo PGEl dissolved in 5Oml of physiological saline was infused intravenously over 5 &titer. The change in the wave length of each patient 5 minutesafter the fomplettion of the infusion was examinined to compare the extensibiity of vessels. stetisticel analysis showed a significaat decrease in the tone of arterial walls h patieob with diff”SC COMcctive tiSS,tCdiSCarCafter trCatment, corn&Wedto patients with neuralgia subsequent to htrpca zoster.

NEOPTERIN Y.OKUBO,

IN SERUM RJRISAWA

FROM

Tokyo Medical

College, Tokyo,

Neopterin

is released in vitro

endotherial

PATIENTS

AND M.KOGA.

cells following

WITH

PSORIASIS.

Department of Dermatology,

JAPAN. from mooocytes,macrophages

interferon-

y stimulation,

and

which is a

product of activated T lymphocytes. Urine and serum neopterin levels have been shown variable neopterin

concentration

in psoriatic

patients. In this study, serum

was measured using radioimmunoassay

in 17

patients with psoriasis and 8 healthy controls. Serum neopterin levels were significantly controls. correlate

Psoriasis

increased

in psoriatic

area and severity

with serum oeopterio

patients

compared

index scores(PAS1)

with

did not

levels

030 CYTOKINE EXFRESSIONIN PEIUPHERAL BLOOD MONONUCLEAR

CEL.L.S FROM PATIENTS WITH ALOPECIA AREATA. K.Katsniri*‘~2~ 1. wepsrtment of Dermatology, Ohs Medical University, Oita sod zDepamneotof Dermatology, Osaka University Medical School, Osaka, Japan.

AllIla@ ~filtratiog lymphocytesin the lesion of alopeciasreataare considered to play ao impcrtant role in the pathogenesis.specific functions of theselymphocytesin the diseasehave beenrarely studied. We studiedspootsneousexpmssiooof IL4 sod lFN-y mRNA io

petiphersl blood mcnombAear cells, whii might reflect characteristics of lyqhqtes io tbe lesiooal akin, from eighteeo patients sod eight healthy individuals using semiquantitative RT-f’CR method.Amplificatioo sigosls

for IL-4 were slightly higher, while Ibasefa IFN-1 were slightly lower in the pstieotsthan in healthy ccnhols. Among the patients.the intensities of smplifioation signals far either IL-4 or IFN-1 io those with severe ~~~~~~~~~~~~~~~~~~~~~~ w-eat3sctivated in ho. Besides, diRefat and EN-y mRNA, llV+ sod Thl oytokii

levels of the -iOn of IL4 tespectively, in the severe

II,.la PRODUCTION BY PERIPHERAL BLOOD MONONUCLEAR PATIENTS WITH GENERALIZED PUSTULAR PSORIASIS &p

CELLS

FROM

and A. Ohkews,a Depanment of Dermatology, Hokkaido University School of Medicine, Sappom. Japan. Several lines of’ clinical and experimental ewdencc suggest that spxific immunological mechanisms are involwd in the pathogenesis of generalized pustular psoriasis (GPP). We dcmonstmti that the scra fmm GPP patients contained higher amounts of ILlh IL-6, IL8 and TNF-a than those of control individuals. It is well known that ILlg can te acause of fercr and Induces othercytokine production. In this study, we examined ILIP production of patients peripheral bleed mononuclear cells (PBMC) and monocytes. PBMC were separated b) Ficoll and adherent momrytes-enriched fractions were further plritied by incubating PBMC on tissue culture plates for 2 hours and removing non-adherent cells. fbesc PBMC and monw~vs-enriched fractions were stimulated by various stimuli for 48 hours and sup;mataants were assayed for ILIe by ELISA. The results showed that LPSsumulated PBMC and monocytes from GPP patients both produced approximately twice more Il.-lp than those from normal individuals. supporting a view that IL-16 plays an imporlnnl role in the pathogcncsis of GPP