- Email: [email protected]
0426 : SarcOptiM, an ImageJ plug-in for cardiomyocyte contractility recording
234
Archives of Cardiovascular Diseases Supplements (2016) 8, 233-234
0082
0426
Nutrition education: what impact in patients with heart failure?
SarcOptiM, an ImageJ plug-in for cardiomyocyte contractility recording
Imad Nouamou *, Mohamed Fouad Khadre, Hind Tahiri, Rachida Habbal CHU Ibn Rochd, Casablanca, Maroc * Corresponding author: [email protected] (Imad Nouamou) Introduction A high sodium diet is a major cause of decompensation and hospitalization in HF patients. Aim To evaluate the impact of specialized nutrition education on biological parameters of HF patients. Patients and methods We proceed to prospective longitudinal study including patients with heart failure in heart failure unit of cardiology department of IBN ROCHD University Hospital of Casablanca (Morocco), stable patients with chronic heart failure were included, not hospitalized for acute heart failure within 3 months prior inclusion, under stable dose diuretics, without significant renal insufficiency (Cr-cl >45ml / min) and without Hyponatremia <130mmol / l. The parameters analyzed were age, gender, biological parameters before and after nutrition education (natremia, natriuresis, kalemia, kaliuresis, creatinine, urinary creatinine, blood urea nitrogen and urinary urea nitrogen). Nutrition education was carried out by a specialized nutritionist trained in education of heart failure. Comparison of biological parameters before and after education was made by SPSS version 17.0 software using the t test for paired sample. Results 44 patients were included in this study, mean age was 58.36 +/– 8 yo, the gender ratio (H / F) = 1,44. It was noted the decrease in average natremia (–1.7 nmol/l) (p<0,05), natriuresis (–8.28 nmol/l) (p<0,05), Kalemia (–0.38 nmol/l) (p<0,05), kaliuresis (–3.27 nmol/l) (p<0,05), urinary creatinine (–9.09 nmol/l) (p>0,05) after the nutrition education. Conclusion Nutrition Education appears to be beneficial in patients with stable HF. We suggest in the light of the results and data from the literature the interest of nutrition education for patients with stable heart failure and its possible introduction into the gold standard of care for patients in the heart failure. The author hereby declares no conflict of interest
Accurate measurement of cardiomyocyte contractility is a critical issue for scientists working on cardiac physiology and physiopathology of diseases implying contraction impairment such as heart failure. The most reliable method for the evaluation of the cardiomyocyte contraction amplitude and kinetics consists in the measurement of sarcomere shortening. The few tools available for this are always tied to dedicated video cameras and none is freely distributed. Here, we describe SarcOptiM, a free open-source plug-in developed in Java for ImageJ/Fiji image analysis platform of the NIH, running under Windows, Mac and Linux operating systems. It computes sarcomere length via Fourier transform analysis of video frames captured or displayed in ImageJ and thereby allows real-time or offline sarcomere measurements. This plug-in has been validated with experimental recordings of rat ventricular cardiomyocyte in several conditions including beta adrenergic stimulation, and with synthetic videos of contracting cardiomyocyte with known features. SarcOptiM is not tied to a specific hardware contrary to marketed software, and works with all digital and analogical video cameras interfaced with ImageJ. Thus, the video camera can be chosen according to the experimental needs. The sampling frequency of the sarcomere length recording is set by the video camera frame rate; a sampling frequency of 1000 Hz is easily reachable with a mid-range costless video camera. Moreover, SarcOptiM overcomes some common problems such as displacements related artifacts and orientation of the cardiomyocyte in the visual field. In conclusion, SarcOptiM is a free, powerful and user-friendly tool for researchers studying cardiomyocyte contractility in all species including human. The author hereby declares no conflict of interest
© Elsevier Masson SAS. All rights reserved.
Côme Pasqualin *, François Gannier, Angèle Yu, Claire O. Malécot, Pierre Bredeloux, Véronique Maupoil STIM CNRS ERL 7368, Physiologie des Cellules Cardiaques et Vasculaires, Tours, France * Corresponding author: [email protected] (Côme Pasqualin)
Archives of Cardiovascular Diseases Supplements (2016) 8, 233-234
0082
0426
Nutrition education: what impact in patients with heart failure?
SarcOptiM, an ImageJ plug-in for cardiomyocyte contractility recording
Imad Nouamou *, Mohamed Fouad Khadre, Hind Tahiri, Rachida Habbal CHU Ibn Rochd, Casablanca, Maroc * Corresponding author: [email protected] (Imad Nouamou) Introduction A high sodium diet is a major cause of decompensation and hospitalization in HF patients. Aim To evaluate the impact of specialized nutrition education on biological parameters of HF patients. Patients and methods We proceed to prospective longitudinal study including patients with heart failure in heart failure unit of cardiology department of IBN ROCHD University Hospital of Casablanca (Morocco), stable patients with chronic heart failure were included, not hospitalized for acute heart failure within 3 months prior inclusion, under stable dose diuretics, without significant renal insufficiency (Cr-cl >45ml / min) and without Hyponatremia <130mmol / l. The parameters analyzed were age, gender, biological parameters before and after nutrition education (natremia, natriuresis, kalemia, kaliuresis, creatinine, urinary creatinine, blood urea nitrogen and urinary urea nitrogen). Nutrition education was carried out by a specialized nutritionist trained in education of heart failure. Comparison of biological parameters before and after education was made by SPSS version 17.0 software using the t test for paired sample. Results 44 patients were included in this study, mean age was 58.36 +/– 8 yo, the gender ratio (H / F) = 1,44. It was noted the decrease in average natremia (–1.7 nmol/l) (p<0,05), natriuresis (–8.28 nmol/l) (p<0,05), Kalemia (–0.38 nmol/l) (p<0,05), kaliuresis (–3.27 nmol/l) (p<0,05), urinary creatinine (–9.09 nmol/l) (p>0,05) after the nutrition education. Conclusion Nutrition Education appears to be beneficial in patients with stable HF. We suggest in the light of the results and data from the literature the interest of nutrition education for patients with stable heart failure and its possible introduction into the gold standard of care for patients in the heart failure. The author hereby declares no conflict of interest
Accurate measurement of cardiomyocyte contractility is a critical issue for scientists working on cardiac physiology and physiopathology of diseases implying contraction impairment such as heart failure. The most reliable method for the evaluation of the cardiomyocyte contraction amplitude and kinetics consists in the measurement of sarcomere shortening. The few tools available for this are always tied to dedicated video cameras and none is freely distributed. Here, we describe SarcOptiM, a free open-source plug-in developed in Java for ImageJ/Fiji image analysis platform of the NIH, running under Windows, Mac and Linux operating systems. It computes sarcomere length via Fourier transform analysis of video frames captured or displayed in ImageJ and thereby allows real-time or offline sarcomere measurements. This plug-in has been validated with experimental recordings of rat ventricular cardiomyocyte in several conditions including beta adrenergic stimulation, and with synthetic videos of contracting cardiomyocyte with known features. SarcOptiM is not tied to a specific hardware contrary to marketed software, and works with all digital and analogical video cameras interfaced with ImageJ. Thus, the video camera can be chosen according to the experimental needs. The sampling frequency of the sarcomere length recording is set by the video camera frame rate; a sampling frequency of 1000 Hz is easily reachable with a mid-range costless video camera. Moreover, SarcOptiM overcomes some common problems such as displacements related artifacts and orientation of the cardiomyocyte in the visual field. In conclusion, SarcOptiM is a free, powerful and user-friendly tool for researchers studying cardiomyocyte contractility in all species including human. The author hereby declares no conflict of interest
© Elsevier Masson SAS. All rights reserved.
Côme Pasqualin *, François Gannier, Angèle Yu, Claire O. Malécot, Pierre Bredeloux, Véronique Maupoil STIM CNRS ERL 7368, Physiologie des Cellules Cardiaques et Vasculaires, Tours, France * Corresponding author: [email protected] (Côme Pasqualin)