047 Gene expression of matrix metalloproteinases in squamous cell carcinoma

JSID Abstracts

189

043

046

ALTERED GENE EXPRESSIONSIN PROGRESSIVESYSTEMIC SCLEROSIS(PSS) FIBROBLASTS. M. One’. M. Ono and H. Ucki. Dcpartmcnt of Dermatology, Kawasaki Medical School, Kurashiki, Japan. Various functional abnormalities of fibroblasts have been invcstigatcd in PSS. Most of these abnormalities are known to be originated at the lcvcl of gene expression. In this study, we aimed to identify gene(s) which transcriptionally altered in PSSfibroblasts. Rcvcrse transcription followed by Polymerasc chain reaction was cmploycd 10 display the differential expression of mRNAs. As a prcscnt result, we have observed 72 altered (expressed or defected) clones in cultured PSS fibroblasts as compared to normal control. DNA sequencing will be pursued. This study may provide an information for pathogenicgene(s)of PSS.

REGULATION OF MATRIX METALLOPROTEJNASES &IMP) EXPRESSION IN CUTIS LAXA FIBROBLASTS: UPREGULATION OF MMP-1, MMP3 AND MMP-9 GENES BUT NOT OF MMF-2 GENE . A. Hatemochi, K. Kuroda and H. Shinkai. Departmentof Dermatology, Chiba University Schoolof Medicine,Chiba, Japan Geneexpression levels of matrix metaUoproteinases(MMP)-1.2.3 and 9 and clastin in cutis laxa tibroblasts were studiedby measuringmRNA levels in three strains from patients with congenital cutis laxa and comparingthem with age- matchedhealthy subjects. Levels of MMP-1, MMP-3 andMMI-9 mRNAs were prominentlyupregulatedin all cutis laxa fibroblast strains, whenas mRNA levels of MMF-2 were unalteredas well as those of GAPDH. Levels of elastin mRNA were shown to be downre@ated in thesestrains as previously described. Since regulatory regions of MMP-1, MMP-3, MMP-9 and elastin genescontain the TPAresponsiveelement,it is suggestedthat tbc regulationmechanismof these genesthrough this element might be importaot io pathogenesisof this disease. Work is in progress to detectprotein production levels of these genesin cutis laxa fibroblasts.

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047

HYOFIBROBLASTIC DIFFERENTIATION OF FIBROBLASTS IN FIBROSINC SKIN DISEASES. Y. Yaaamolo*. K. Karlya. K. Miyoshl and il. Xodama. Department of Koch1 Medical School. Nankoku. Dermatology, JSPSII. Kochl, ic Recent studies Indicate that myolibroblast firole In a variety of cells play an ess entlal myolih roIn the current study, hrotlc diseases. lation of librablasts in fib IOblastlc different 11c skin diseases was investigated by inmunoh islochemlstry uslag antibodies against the markers for myofihrohlasts. Expression of o! -smooth ouscle aet~n was evldenr III the fibroblaslic cells Induced 01 of actively flhrosing lesions. TCF-8 -smooth muscle act!” expression on cultured human fihroblasls in vitro.

GENE EXPRESSION OF MATRIX METALu3pRoTEINASBS IN SQUAMOUS CELL CARCINOMA. R. l?&ifuii, Y. Sakai. A. Hatamochiand H. Shinkai. mnnt of Dermatology,Chiba University School of Medicine, Chiba, Japan We examinedgeneexpressionof mahix metallopro4einases (h4MP-1, MMF’-2 and MMP-3) in squamouscell carcinoma(SCC) by in siru hybridization technique. delve casesof SCC were examinedusing form&n-fixed, paraffin+mbeddedsectionsanddigoxigenio-labeledRNA probes. Both tumor cells and tibroblast-l&e cells of the stmmaexpressed mRNAs of MMP-1 andMMP-3. ‘I& expressionof MMP-3 was observedin a smallernumberof cells localizedat the tumoralI stromal interfacearea. The geneexpressionof MMF-2 was found in fibmblasts scatteredmore widely throughoutthe stroma. Thesefindings suggestthe tumor cells leadthe stromalcells to producesomeMMPs andeach enzymeplays an important,uniquerole in tllmor invasion.

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INTERFERON- ?’ EXPRESSION H.l. Kwon Keimyung

IN

RBGULATES HUMAN

SKIN

STROMELYSIN-1

GRNNE

FIBROBLASTS. Y.W. Dermatnlw~.

and K.S. Lee Department of University

School

of Medicine,

Taegu.

RYOO. The

Korea.

Stromelysin plays a major role in different aspects of the extrcelhdar matrix degradation. The interferon have potent cellular modulation and

effects such as inhibition of cell proliferation, of cell differentiation. depending on the cell type

the dose

of interferon.

biosynthesis

by

dermal

and cost.4 transcriptional

chondrocytes. regulation

Especially and

synovial This

of human

by INl-

INF-

J

fibroblasts study was stromelysin-1

inhibits

collagen

and articular examed that gene

induced

T and TGF- 8 using Northern blot analysis, CAT assay shift assay. Our results show that INF-y and gel up-regulates stmmelysin-I gene expression in cultured human skin fihroblast that may be mediated by AP-1 transcriptional factor

on transcriptional

stage.

EFFECI?? OF RECOMBlNAW HUMAN TISSUE INHIBmlR OF METALLOPROTElNASE.2 (rk-TIMP-2) ON WOUND HEALJNG. K Terns&i*‘. T. Km&i’. T. Oku’. A Shinnnaw? .md H,DeParbn’Depsrbnwt d Demmtology, Kagmkima University Faculty of Medicine, Kagoshima, Japan md~iopharmaceuticpl Dcqnment, Fuji Cixmical Industries, Ltd., Toyama, Japan.