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078 TH2-dominant cytokine profile in chronic lesions induced by repeated elicitation of contact hypersensitivity
194
JSID Abstracts
073
076
EFFECT OF POPULATION DENSITY ON ELICITATION OF CONTACT HYPERSENSITIVITY. -oil. T. Tsucl&&R& win* J Kovd ’ The Life Science Laboratories, Shiseido, Tokyo, Japan, ‘Department of Dermatology, Cornell University, New York, USA To reveal effects of sqcial stress on immune reactions in the skin allergic contact hypersensitivity (CH) was elicited in mice housed at various population density. Female C57BU6CRj mice were housed at the density of 5 per cage and sensitized by application of TNCB on the dorsal skin. After 4 days the density was changed to 1,5, or 10 per cage. On the next day mice were challenged at ears by painting of TNCB. Ear swelling was measured and the blood was collected to assay for corticosterone after 24 hours. Corticosterone increased as the density increased, demonstrating that high density is stressful for animals. The elicitation of the CH was less in mice housed at high density (i/cage: O.l78mm, 5: 0.134, 10: 0.088). These results suggest the regulation of skin immune reactions by social stress.
REGULATORY HBCHANISY OF iTI!ROID MDUCBD AUOMBNTAI’ION OF CONTACT HYPERSBNSITIVITY BY KEMTINOCYTE DBNVED CYTOKINES. Ken kawa Yawbiro Mivazaki. T&biro Satob. Hiron Yokozcki. Icbiro ~.Depmmcm la of Dcnnamlogy.Tokyo Mcdlal And Dental University,School of Medicine, Tokyo,Japan. PURPOSE We have reported that long-term topical steroid augmznta cant&2 hypersensitivity reactionin mice. Tbcrcfore, wesW%cd the mccbanism d down-regulation of thissteroid induced augmentation of contact hypersensitivity rcauionMRTHOD Balblc mice were applied on the flank skin with 50 ~1 d Diflucatmn solution in ethanol on alternate day for 9 times. On day 14 when mice received 7th applicationa of diilucortmn, each mouse was scnsirized with 0.5% DhlT3 on same site with diflucorlron (flank) 01 opposite site(back). On day 19, one day after the last application, cdcb mouse was challenged with 0.15% DMB on the righl pinna. Next wcaullcd the mwbuism d downregulalion of this response, by local injection oflL10, Ul,9rcceptor antagoairt, cstncU of kcratinocvtc or fibroblast ccl1 line before cballcnw tcsr.RESULT Ibc down-reti of augmented skin rcanion was observed by lbe pretreatment of IL1 B rcaptor~upnia or cx1ra.cLsof kcratinocytc. Partial down-regulation was observed by U.10, 01 extnctn d fibroblast. CONCLUSION Augmeticd contacthypersensitivity reauion by topical steroid wasdown-regulated by ILlO, lLl,9 receptor antagonist cr varioussubstance duiwd from kcratioocytc or fibroblasr. Topical swoid might modulate the prodwtlon dtbae inhibilory molecules by the resident cells oftbe skin resulting in augmcUcd skin ruc4lon.
074
077 CYTOKINE PROFILE OF REGIONAL LYMPH NODE CELLS IN MURINE CONTACT HYPERSJ3NSITIVI’N. M. Natsuaki’ ‘, *, K. Abe’ and Y. KitanoZ. ‘Department of Dermatology. Saiseikai Suita Hospital, Osaka. *Department of Dermatology, Byogo College of Medicine, Ilyogo. Japan. In this study, we investigated what kind of cytokines are produced by regional lymph node stimulated with a bacterial superantigen cells or a specific hapten in murine contact hypersensitiVity(CH). BALB/c mice were sensitized with dinitrofluorobenzene and the r.egional lymph node cells were cultured with a specific hapten. Several cytokines in the culture supernatant were measured. Interferon-r (IFN-y ) was mainly produced by the lymph node cells. Staphylococcal enterotoxin B (SEB) may affect CH responses as a immunomodulator because normal lymph node cells stimulated with SEB produced some cytokines including IFN-7 .
078 THZ-DOMINANT CYTOKINB ‘PROFILE IN CHRONIC LESIONS INDUCED BY RBPEATED BLICflXTION OF CONTAClHYPBRSKNSfl’MTY. H. KITAOAKI”% T. KITAZAWL K. MTANABI and T. SHIOHARA ‘Dcpmtmwt School of Medicine. Tokyo, Jqn. Flmnnacwlicdl co.. Hyop, Japan.
of Dmsatelqy, lAku Rexarch
Kyoda Universi~ Institute, Owh
WPI &Icuively uprcgul8ted i;; the -dwc& iesiti skib i;; 111 aotlgon-specific frshiw. wbcreur TM-type cytokinc @N-y, IL-2) gear
;~prcsgion expnssion
WIIl pfcdomiMnt
in acute
I&mu.
These
rcaaa
illdicne
Iha
repeated elicitation of contact hypexnumhivity could shift the type of inflammation fmm ml-type to IlQ-typ tiQ b oftea ob&rvcd in chnmic lcsims of allergic &In discas@ such a WC dermatitis.
JSID Abstracts
073
076
EFFECT OF POPULATION DENSITY ON ELICITATION OF CONTACT HYPERSENSITIVITY. -oil. T. Tsucl&&R& win* J Kovd ’ The Life Science Laboratories, Shiseido, Tokyo, Japan, ‘Department of Dermatology, Cornell University, New York, USA To reveal effects of sqcial stress on immune reactions in the skin allergic contact hypersensitivity (CH) was elicited in mice housed at various population density. Female C57BU6CRj mice were housed at the density of 5 per cage and sensitized by application of TNCB on the dorsal skin. After 4 days the density was changed to 1,5, or 10 per cage. On the next day mice were challenged at ears by painting of TNCB. Ear swelling was measured and the blood was collected to assay for corticosterone after 24 hours. Corticosterone increased as the density increased, demonstrating that high density is stressful for animals. The elicitation of the CH was less in mice housed at high density (i/cage: O.l78mm, 5: 0.134, 10: 0.088). These results suggest the regulation of skin immune reactions by social stress.
REGULATORY HBCHANISY OF iTI!ROID MDUCBD AUOMBNTAI’ION OF CONTACT HYPERSBNSITIVITY BY KEMTINOCYTE DBNVED CYTOKINES. Ken kawa Yawbiro Mivazaki. T&biro Satob. Hiron Yokozcki. Icbiro ~.Depmmcm la of Dcnnamlogy.Tokyo Mcdlal And Dental University,School of Medicine, Tokyo,Japan. PURPOSE We have reported that long-term topical steroid augmznta cant&2 hypersensitivity reactionin mice. Tbcrcfore, wesW%cd the mccbanism d down-regulation of thissteroid induced augmentation of contact hypersensitivity rcauionMRTHOD Balblc mice were applied on the flank skin with 50 ~1 d Diflucatmn solution in ethanol on alternate day for 9 times. On day 14 when mice received 7th applicationa of diilucortmn, each mouse was scnsirized with 0.5% DhlT3 on same site with diflucorlron (flank) 01 opposite site(back). On day 19, one day after the last application, cdcb mouse was challenged with 0.15% DMB on the righl pinna. Next wcaullcd the mwbuism d downregulalion of this response, by local injection oflL10, Ul,9rcceptor antagoairt, cstncU of kcratinocvtc or fibroblast ccl1 line before cballcnw tcsr.RESULT Ibc down-reti of augmented skin rcanion was observed by lbe pretreatment of IL1 B rcaptor~upnia or cx1ra.cLsof kcratinocytc. Partial down-regulation was observed by U.10, 01 extnctn d fibroblast. CONCLUSION Augmeticd contacthypersensitivity reauion by topical steroid wasdown-regulated by ILlO, lLl,9 receptor antagonist cr varioussubstance duiwd from kcratioocytc or fibroblasr. Topical swoid might modulate the prodwtlon dtbae inhibilory molecules by the resident cells oftbe skin resulting in augmcUcd skin ruc4lon.
074
077 CYTOKINE PROFILE OF REGIONAL LYMPH NODE CELLS IN MURINE CONTACT HYPERSJ3NSITIVI’N. M. Natsuaki’ ‘, *, K. Abe’ and Y. KitanoZ. ‘Department of Dermatology. Saiseikai Suita Hospital, Osaka. *Department of Dermatology, Byogo College of Medicine, Ilyogo. Japan. In this study, we investigated what kind of cytokines are produced by regional lymph node stimulated with a bacterial superantigen cells or a specific hapten in murine contact hypersensitiVity(CH). BALB/c mice were sensitized with dinitrofluorobenzene and the r.egional lymph node cells were cultured with a specific hapten. Several cytokines in the culture supernatant were measured. Interferon-r (IFN-y ) was mainly produced by the lymph node cells. Staphylococcal enterotoxin B (SEB) may affect CH responses as a immunomodulator because normal lymph node cells stimulated with SEB produced some cytokines including IFN-7 .
078 THZ-DOMINANT CYTOKINB ‘PROFILE IN CHRONIC LESIONS INDUCED BY RBPEATED BLICflXTION OF CONTAClHYPBRSKNSfl’MTY. H. KITAOAKI”% T. KITAZAWL K. MTANABI and T. SHIOHARA ‘Dcpmtmwt School of Medicine. Tokyo, Jqn. Flmnnacwlicdl co.. Hyop, Japan.
of Dmsatelqy, lAku Rexarch
Kyoda Universi~ Institute, Owh
WPI &Icuively uprcgul8ted i;; the -dwc& iesiti skib i;; 111 aotlgon-specific frshiw. wbcreur TM-type cytokinc @N-y, IL-2) gear
;~prcsgion expnssion
WIIl pfcdomiMnt
in acute
I&mu.
These
rcaaa
illdicne
Iha
repeated elicitation of contact hypexnumhivity could shift the type of inflammation fmm ml-type to IlQ-typ tiQ b oftea ob&rvcd in chnmic lcsims of allergic &In discas@ such a WC dermatitis.