- Email: [email protected]
108 New highly effective nanostructured IR-photosensitizers for photodynamic therapy of deeply located and pigmented tumors
S36
Abstracts of the Laser Helsinki 2012 International Congress
106
monoxide and carbon dioxide. The recorded and calculated spectra will be presented. Conclusion: The work proofed positive the possibility of using the femtosecond laser for detection of broadband absorption spectra in the infrared region of the carbon monoxide and carbon dioxide as biomarkers in the human breath. Acknowledgement: The background of the research is supported by the European Commission and Ministry of Education, Youth, and Sports of the Czech Republic project no. CZ.1.05/2.1.00/01. The presentation of the work is supported by the European Commission and Ministry of Education, Youth, and Sports of the Czech Republic project no. CZ.1.07/2.4.00/31.0016.
Tissue oxygenation measurements in vivo A. Butenin 1 , B. Kogan 1 , N. Andronova 2 , E. Treshchalina 2 , E. Lukyanets 1 , G. Vorozhtsov 1. 1 State Research Center “NIOPIK”, Moscow, Russia; 2 Blokhin Russian Cancer Research Center of RAMS, 115478 Moscow, Russia Background: Oxygen tension measurements in tissue are required in some fields of medicine particularly in oncology. Drawback of hard invasive oxygen sensors is their affecting on vasoconstriction and thus oxygen supply. This does not occur in case of liquid sensor injected into tissue. We used phosphorescent liquid sensor for oxygen tension measurements in mice tumors during heating of the last. Material and Methods: Mice BDF with inoculated intramuscular melanoma were used as experimental models. Tumors were heated by radiation of Nd:YAG laser (wave length of 1064 nm, an average power of up to 10 W). Tumor temperature was measured using thermocouple mounted into gilded medical needle (diameter of 0.35 mm). Palladium meso-tetra-(phenyl)-tetrabenzoporphin was dissolved in medical liquid paraffin. This phosphorescent solution (volume of 3–10 mm3 , concentration of 16 mcM) was injected into tumor before session of heating as oxygen tension probe. Pulsed diode laser (wave length of 638 nm, pulse energy and duration of about 0.2 mcJ and 40 ns correspondingly, repetition frequency of 50 Hz) was used for excitation of the probe phosphorescence. Phosphorescence lifetime was digitized and used for oxygen tension determination. This mode has advantage before measurements of phosphorescence intensity because results don' t depend on dye concentration and excitation intensity. Results: Phosphorescence decay was near to exponential. It facilitates processing of measurement results. Oxygen tension dynamics during sessions of heating is presented. Heating of the tumor is accompanied at first by vasodilatation, after that by vasoconstriction. Usually exposure during 15 min at temperature 45°C led to irreversible damage of tumor blood vessels. Conclusion: This method can be used during PDT experiments in vivo as well.
107 Detection of human breath biomarkers by femtosecond laser based absorption spectroscopy O. Cíp 1 , A. Lesundak 1 , Z. Buchta 1 , R. Smid 1 , J. Lazar 1 , A. Randula 2 . 1 Department of Coherence Optics, Institute of Scientific Instruments of the ASCR; 2 First Department of Surgery, Faculty of Medicine, Masaryk University, Czech Republic Background: The human breath consists of hundreds of molecules of gasses. An increased concentration of more of them indicates certain diseases or symptoms. Because each type of these molecules has a different absorption spectrum of wavelengths it can be indicated by supercontinuum light generated by pulsed femtosecond lasers. In the infrared region covered by Er+ doped fibre lasers, there are more than ten biomarkers which can be monitored. These markers are defined as values of concentration of different isotopes of carbon dioxide and carbon monoxide. In this case if the patient is infected with any bacteria or the patient is smoker these markers sign that reality. Material and Methods: The presenting work covers a pilot experiment including the generation of the femtosecond pulse probe laser beam and a detection chain based on cross-correlation analysis of the passing femtosecond pulsed beam trough the specimen of the breath. The chain uses the absorption spectroscopy technique. We put together the pilot experimental set-up where detection part utilizes a Michelson interferometer with movable measuring mirror. The caught optical signals are calculated by fast Fourier transform from the time to wavelength domain. Results: The first caught spectra of the femtosecond beam passing trough the specimens show absorption lines of isotopes of carbon
108 New highly effective nanostructured IR-photosensitizers for photodynamic therapy of deeply located and pigmented tumors M.A. Grin 1 , I.V. Pantjushenko 1 , R.I. Reshetnikov 1 , G.A. Meerovich 2 , A.F. Mironov 1 , A.V. Geynits 3 . 1 Moscow State University of Fine Chemical Technologies named after Lomonosov, Moscow, Russia; 2 Institute of General Physics named after Prokhorov, Russian Academy of Sciences, Moscow, Russia; 3 State Research and Clinical Center for Laser Medicine of Healthcare Ministry of Russia, Moscow, Russia Introduction: Development of new techniques for treating oncologic diseases is a priority direction of medicine in the XXIth century. One of such techniques is photodynamic therapy (PDT) as an example of binary therapy when two non-toxic agents (photosensitizer and laser light) brought to the target-cell separately meet there and generate cytotoxic substances – oxygen active forms (OAF) and, first of all, singlet oxygen. Natural chlorophylls and their derivatives when absorbing light in the range 700–800 nm become photosensitizers (PS) of near IR range and give PDT effects in tissues at 20 mm depth. However, chlorins and bacteriochlorins have a limited application as PS because of high hydrophobic property, low chemical and photo- stability, moderate accumulation selectivity in cancer cells. It dictates a necessity to develop stable chlorin and bacteriochlorin derivatives with better spectral parameters, increased hydrophilic properties to be solved in polar solvent and water which have a considerable affinity to tumors ... Materials and Methods: Our researches were performed in vivo in mice with Erlich tumor (strain ELD), melanoma B16 and human melanoma Mel7. Strains for transplantable tumors were taken from the bank of tumor strains in N.N. Blokhin Cancer Research Center (Moscow). Photosensitizers were injected to the mice with a single intravenous injection on the 7th day after the transplantation. PS accumulation level was assessed in vivo with a spectroscopic technique of diffuse reflection. So as to determine accumulation selectivity the level of preparation accumulation was measured with the same technique in normal tissue and then a selectivity index was calculated. Parameters of tumor growth inhibition and life-span increase were obtained under PS dosage 0.5–1 mg/kg of body weight, laser light wavelength 797 nm, power density 200 mW/cm2 , exposure time 20 min. Results: A regioselective synthesis of bacteriopurpurinimides containing hydroxyl and aloxi-groups in different microcycle positions has been performed; effects of pigment structures at their biologic effectiveness have been shown. A leader formation has been found and, its nanostructured (liposomal and micellar) injection forms have been developed. In animal experimentation three basic parameters of biologic activity have been studied. These parameters included a coefficient of active substance accumulation in the tumor (selectivity index), tumor growth inhibition (TGI) and lifespan increase (LSI). Experimental PDT of mice melanoma B 16T with PS in the form of micellar dispersion based on Kremophore EL has been found out to cause growth inhibition with TGI index equal to 91% and LSI index equal to 61.3%. Studies on photoinduced activity of the proposed PS for treating human melanoma Mel7 in athymic mice have shown its high ef-
Abstracts of the Laser Helsinki 2012 International Congress fectiveness with TGI index about 96%. A good pharmacokinetics of leader bacteriopurpurinimid including a maximal preparation accumulation in the tumor during 5 hours after an injection with selectivity index up to 11 and a high photodynamic activity make this compound quite a perspective one for further studies. Conclusion: The obtained findings allow proposing a new effective infrared photosensitizer based on etoxibacteriopurpurinimid for melanoma PDT; its nanostructured injection form has been developed.
109 Use of laser radiation in regulation and measurement of nano-particle N. Kokodiy 1,2 , V. Timaniyk 1 . 1 Department of Physics, National University of Pharmacy, Kharkov, Ukraine; 2 Department of Quantum Radio-physics, Kharkov National University, Kharkov, Ukraine Introduction: Nanotechnology is the part of science and technique, which is developing intensely the last years. The using of nanoparticles in medicine and pharmacy is one of directions in science. The nano-particles can be carriers of preparation in the blood of man, strengtheners of their action etc. This is necessary to know a shape, size and other parameters (density, conductivity, dielectric and magnetic permeances, refracting index in optical range). This is possible to find several of these parameters by methods, which are basing on the results of analysis of interaction of laser or un-coherent optical radiation with nano-particles. The laser radiation can be using for acting on nano-particles with the purpose of rise of their activity by interaction with biology object and for measuring of their parameters. Material and Methods: 1. This was investigated the interaction of laser radiation with cylindrical nano-particles. The length of particles is much bigger than diameter. The material of particles is carbon. The results were confirmed the presents of effect of very strong absorbing of electromagnetic radiation by particles. The effective size of absorbing can be exceed the geometric size in several hundreds or thousands of one times. There was done the calculation of the absorbing of laser radiation by carbon nano-particles, which are chaotically distributing in a liquid. The results are show on the possibility of effective transmission of laser energy to nano-particles. 2. The methods of measuring of geometric and optical parameters of nano-particles are proposed. These methods are basing by interaction of laser radiation with particles. 2.1. This is investigated the shape of diagram of scattering of laser radiation by nano-particles. The direction of polarization of scattering radiation was investigated also and it was found in several point of space. It was used for measuring of size of nano-particles. 2.2. The spectral dependence of extinction of laser radiation in liquid with nano-particles was investigated experimentally. The mathematical processing of results was allowed to find the sizes of particles, their refracting index and concentration. 2.3. The experiments of measuring of sizes of nano-particles by the method of dynamical light scattering (DLS) were done. The fluctuations of intensity of scattering laser radiation by Brownian movement were measured. Results: 1. Cylindrical nano-particles can very effectively co-absorb the laser radiation. This effect can use for the transmission of laser radiation energy to particles. 2. The optical methods of measuring of sizes of nano-particles and their optical parameters are proposing. Conclusion: – The laser radiation can be used for measuring of size of nanoparticles and their optical parameters.
S37 – It is possible. – It is show that possibly very effective interaction of laser radiation by cylindrical nano-particles.
110 Spectral luminescent properties of methylpheophorbid a in molecular and nano forms V.B. Loshchenov 1,2 , S.Yu. Vasilchenko 1,2 , A.V. Ryabova 1,2 , G.V. Ponomarev 3 . 1 GPI RAS, Moscow, Russia; 2 JSC “BIOSPEC” Moscow, Russia; 3 IBMC RAMS, Moscow, Russia Possibilities of pheophorbide a and its derivatives as a photoactive substances for photodynamic therapy have been being investigated since the 80' s of the past century. These substances satisfy the main requirements for photosensitizers as high tumor-tropism and intensive absorption in the red spectrum region. The work presents results of investigation of optical-spectral properties of methylpheophorbide a in molecular and nanoforms. The nanoparticles show the specific optical features. So methylpheophorbide a nanoparticles absorption spectrum shows the high absorption coefficient in the all ranges of the visible spectrum region with the exception of the absorption line of the monomolecular methylpheophorbide a form. The absorption spectra of methylpheophorbide a nanoparticles and its monomolecular form are inversely wavelength axis-symmetrical and fluorescence spectra are inversely intensity axis-symmetrical. The fluorescence spectrum of methylpheophorbide a nanoparticles has an intensive peak in the near-IR spectrum region. The latter allows one to use methylpheophorbide a nanoparticles as an effective agent for IR-imaging of deeper layers of biotissue. Optical-spectral properties of pheophorbide a and pyropheophorbide in molecular and nano- forms will also be discussed in the report. The results on biodistribution and photodynamic activity of methylpheophorbide a nanoparticles at the cell level and in experimental animals will also be presented. The experiments on the Ehrlich`s carcinoma cell line with the use of laser femtosecond confocal microscopy methods have revealed that non-phototoxic methylpheophorbide a nanoparticles inject phototoxic methylpheophorbide a molecules into the tumor cells when the nanoparticles contact with tumor cell membrane. This makes it possible to fulfil highly selective diagnostics and therapy of oncological diseases.
111 Ytterbium porphyrin complexes and their conjugates with nanoparticles for fluorescent tumour diagnostics A.F. Mironov 1 , V.D. Rumyantseva 1 , A.V. Ivanov 2 , N.G. Hlebtzeov 3 , I.P. Shilov 4 , A.V. Geynits 5 . 1 Moscow State University of Fine Chemical Technologies named after Lomonosov, Moscow, Russia; 2 N.N. Blokhin Cancer Research Center, Moscow, Russia; 3 Saratov State University named after Chernyshevsky, Saratov, Russia; 4 Institute of Radioengineering and Radioelectronics named after Kotelnikov, Moscow, Russia; 5 State Research and Clinical Center for Laser Medicine of Healthcare Ministry of Russia, Moscow, Russia Introduction: One of the perspective directions in early diagnostics of malignant neoplasms which can be revealed endoscopically is fluorescent diagnostics with ytterbium porphyrin complexes. They have an intensive fluorescence in the near IR-spectrum in the so-called “window of tissue transparency”; they accumulate well in tumors, practically do not generate singlet oxygen what is an important advantage comparing to photosensitizers traditionally used for PDT in onologic lesions because ytterbium porphyrin complexes do not cause tissue destruction which is an adverse effect in this case. An effective delivery of ytterbium photosensitizer complexes (Yb-PsC) to a target-tumour is successfully realized due to the formation of conjugates with nanoparticle of definite size.
Abstracts of the Laser Helsinki 2012 International Congress
106
monoxide and carbon dioxide. The recorded and calculated spectra will be presented. Conclusion: The work proofed positive the possibility of using the femtosecond laser for detection of broadband absorption spectra in the infrared region of the carbon monoxide and carbon dioxide as biomarkers in the human breath. Acknowledgement: The background of the research is supported by the European Commission and Ministry of Education, Youth, and Sports of the Czech Republic project no. CZ.1.05/2.1.00/01. The presentation of the work is supported by the European Commission and Ministry of Education, Youth, and Sports of the Czech Republic project no. CZ.1.07/2.4.00/31.0016.
Tissue oxygenation measurements in vivo A. Butenin 1 , B. Kogan 1 , N. Andronova 2 , E. Treshchalina 2 , E. Lukyanets 1 , G. Vorozhtsov 1. 1 State Research Center “NIOPIK”, Moscow, Russia; 2 Blokhin Russian Cancer Research Center of RAMS, 115478 Moscow, Russia Background: Oxygen tension measurements in tissue are required in some fields of medicine particularly in oncology. Drawback of hard invasive oxygen sensors is their affecting on vasoconstriction and thus oxygen supply. This does not occur in case of liquid sensor injected into tissue. We used phosphorescent liquid sensor for oxygen tension measurements in mice tumors during heating of the last. Material and Methods: Mice BDF with inoculated intramuscular melanoma were used as experimental models. Tumors were heated by radiation of Nd:YAG laser (wave length of 1064 nm, an average power of up to 10 W). Tumor temperature was measured using thermocouple mounted into gilded medical needle (diameter of 0.35 mm). Palladium meso-tetra-(phenyl)-tetrabenzoporphin was dissolved in medical liquid paraffin. This phosphorescent solution (volume of 3–10 mm3 , concentration of 16 mcM) was injected into tumor before session of heating as oxygen tension probe. Pulsed diode laser (wave length of 638 nm, pulse energy and duration of about 0.2 mcJ and 40 ns correspondingly, repetition frequency of 50 Hz) was used for excitation of the probe phosphorescence. Phosphorescence lifetime was digitized and used for oxygen tension determination. This mode has advantage before measurements of phosphorescence intensity because results don' t depend on dye concentration and excitation intensity. Results: Phosphorescence decay was near to exponential. It facilitates processing of measurement results. Oxygen tension dynamics during sessions of heating is presented. Heating of the tumor is accompanied at first by vasodilatation, after that by vasoconstriction. Usually exposure during 15 min at temperature 45°C led to irreversible damage of tumor blood vessels. Conclusion: This method can be used during PDT experiments in vivo as well.
107 Detection of human breath biomarkers by femtosecond laser based absorption spectroscopy O. Cíp 1 , A. Lesundak 1 , Z. Buchta 1 , R. Smid 1 , J. Lazar 1 , A. Randula 2 . 1 Department of Coherence Optics, Institute of Scientific Instruments of the ASCR; 2 First Department of Surgery, Faculty of Medicine, Masaryk University, Czech Republic Background: The human breath consists of hundreds of molecules of gasses. An increased concentration of more of them indicates certain diseases or symptoms. Because each type of these molecules has a different absorption spectrum of wavelengths it can be indicated by supercontinuum light generated by pulsed femtosecond lasers. In the infrared region covered by Er+ doped fibre lasers, there are more than ten biomarkers which can be monitored. These markers are defined as values of concentration of different isotopes of carbon dioxide and carbon monoxide. In this case if the patient is infected with any bacteria or the patient is smoker these markers sign that reality. Material and Methods: The presenting work covers a pilot experiment including the generation of the femtosecond pulse probe laser beam and a detection chain based on cross-correlation analysis of the passing femtosecond pulsed beam trough the specimen of the breath. The chain uses the absorption spectroscopy technique. We put together the pilot experimental set-up where detection part utilizes a Michelson interferometer with movable measuring mirror. The caught optical signals are calculated by fast Fourier transform from the time to wavelength domain. Results: The first caught spectra of the femtosecond beam passing trough the specimens show absorption lines of isotopes of carbon
108 New highly effective nanostructured IR-photosensitizers for photodynamic therapy of deeply located and pigmented tumors M.A. Grin 1 , I.V. Pantjushenko 1 , R.I. Reshetnikov 1 , G.A. Meerovich 2 , A.F. Mironov 1 , A.V. Geynits 3 . 1 Moscow State University of Fine Chemical Technologies named after Lomonosov, Moscow, Russia; 2 Institute of General Physics named after Prokhorov, Russian Academy of Sciences, Moscow, Russia; 3 State Research and Clinical Center for Laser Medicine of Healthcare Ministry of Russia, Moscow, Russia Introduction: Development of new techniques for treating oncologic diseases is a priority direction of medicine in the XXIth century. One of such techniques is photodynamic therapy (PDT) as an example of binary therapy when two non-toxic agents (photosensitizer and laser light) brought to the target-cell separately meet there and generate cytotoxic substances – oxygen active forms (OAF) and, first of all, singlet oxygen. Natural chlorophylls and their derivatives when absorbing light in the range 700–800 nm become photosensitizers (PS) of near IR range and give PDT effects in tissues at 20 mm depth. However, chlorins and bacteriochlorins have a limited application as PS because of high hydrophobic property, low chemical and photo- stability, moderate accumulation selectivity in cancer cells. It dictates a necessity to develop stable chlorin and bacteriochlorin derivatives with better spectral parameters, increased hydrophilic properties to be solved in polar solvent and water which have a considerable affinity to tumors ... Materials and Methods: Our researches were performed in vivo in mice with Erlich tumor (strain ELD), melanoma B16 and human melanoma Mel7. Strains for transplantable tumors were taken from the bank of tumor strains in N.N. Blokhin Cancer Research Center (Moscow). Photosensitizers were injected to the mice with a single intravenous injection on the 7th day after the transplantation. PS accumulation level was assessed in vivo with a spectroscopic technique of diffuse reflection. So as to determine accumulation selectivity the level of preparation accumulation was measured with the same technique in normal tissue and then a selectivity index was calculated. Parameters of tumor growth inhibition and life-span increase were obtained under PS dosage 0.5–1 mg/kg of body weight, laser light wavelength 797 nm, power density 200 mW/cm2 , exposure time 20 min. Results: A regioselective synthesis of bacteriopurpurinimides containing hydroxyl and aloxi-groups in different microcycle positions has been performed; effects of pigment structures at their biologic effectiveness have been shown. A leader formation has been found and, its nanostructured (liposomal and micellar) injection forms have been developed. In animal experimentation three basic parameters of biologic activity have been studied. These parameters included a coefficient of active substance accumulation in the tumor (selectivity index), tumor growth inhibition (TGI) and lifespan increase (LSI). Experimental PDT of mice melanoma B 16T with PS in the form of micellar dispersion based on Kremophore EL has been found out to cause growth inhibition with TGI index equal to 91% and LSI index equal to 61.3%. Studies on photoinduced activity of the proposed PS for treating human melanoma Mel7 in athymic mice have shown its high ef-
Abstracts of the Laser Helsinki 2012 International Congress fectiveness with TGI index about 96%. A good pharmacokinetics of leader bacteriopurpurinimid including a maximal preparation accumulation in the tumor during 5 hours after an injection with selectivity index up to 11 and a high photodynamic activity make this compound quite a perspective one for further studies. Conclusion: The obtained findings allow proposing a new effective infrared photosensitizer based on etoxibacteriopurpurinimid for melanoma PDT; its nanostructured injection form has been developed.
109 Use of laser radiation in regulation and measurement of nano-particle N. Kokodiy 1,2 , V. Timaniyk 1 . 1 Department of Physics, National University of Pharmacy, Kharkov, Ukraine; 2 Department of Quantum Radio-physics, Kharkov National University, Kharkov, Ukraine Introduction: Nanotechnology is the part of science and technique, which is developing intensely the last years. The using of nanoparticles in medicine and pharmacy is one of directions in science. The nano-particles can be carriers of preparation in the blood of man, strengtheners of their action etc. This is necessary to know a shape, size and other parameters (density, conductivity, dielectric and magnetic permeances, refracting index in optical range). This is possible to find several of these parameters by methods, which are basing on the results of analysis of interaction of laser or un-coherent optical radiation with nano-particles. The laser radiation can be using for acting on nano-particles with the purpose of rise of their activity by interaction with biology object and for measuring of their parameters. Material and Methods: 1. This was investigated the interaction of laser radiation with cylindrical nano-particles. The length of particles is much bigger than diameter. The material of particles is carbon. The results were confirmed the presents of effect of very strong absorbing of electromagnetic radiation by particles. The effective size of absorbing can be exceed the geometric size in several hundreds or thousands of one times. There was done the calculation of the absorbing of laser radiation by carbon nano-particles, which are chaotically distributing in a liquid. The results are show on the possibility of effective transmission of laser energy to nano-particles. 2. The methods of measuring of geometric and optical parameters of nano-particles are proposed. These methods are basing by interaction of laser radiation with particles. 2.1. This is investigated the shape of diagram of scattering of laser radiation by nano-particles. The direction of polarization of scattering radiation was investigated also and it was found in several point of space. It was used for measuring of size of nano-particles. 2.2. The spectral dependence of extinction of laser radiation in liquid with nano-particles was investigated experimentally. The mathematical processing of results was allowed to find the sizes of particles, their refracting index and concentration. 2.3. The experiments of measuring of sizes of nano-particles by the method of dynamical light scattering (DLS) were done. The fluctuations of intensity of scattering laser radiation by Brownian movement were measured. Results: 1. Cylindrical nano-particles can very effectively co-absorb the laser radiation. This effect can use for the transmission of laser radiation energy to particles. 2. The optical methods of measuring of sizes of nano-particles and their optical parameters are proposing. Conclusion: – The laser radiation can be used for measuring of size of nanoparticles and their optical parameters.
S37 – It is possible. – It is show that possibly very effective interaction of laser radiation by cylindrical nano-particles.
110 Spectral luminescent properties of methylpheophorbid a in molecular and nano forms V.B. Loshchenov 1,2 , S.Yu. Vasilchenko 1,2 , A.V. Ryabova 1,2 , G.V. Ponomarev 3 . 1 GPI RAS, Moscow, Russia; 2 JSC “BIOSPEC” Moscow, Russia; 3 IBMC RAMS, Moscow, Russia Possibilities of pheophorbide a and its derivatives as a photoactive substances for photodynamic therapy have been being investigated since the 80' s of the past century. These substances satisfy the main requirements for photosensitizers as high tumor-tropism and intensive absorption in the red spectrum region. The work presents results of investigation of optical-spectral properties of methylpheophorbide a in molecular and nanoforms. The nanoparticles show the specific optical features. So methylpheophorbide a nanoparticles absorption spectrum shows the high absorption coefficient in the all ranges of the visible spectrum region with the exception of the absorption line of the monomolecular methylpheophorbide a form. The absorption spectra of methylpheophorbide a nanoparticles and its monomolecular form are inversely wavelength axis-symmetrical and fluorescence spectra are inversely intensity axis-symmetrical. The fluorescence spectrum of methylpheophorbide a nanoparticles has an intensive peak in the near-IR spectrum region. The latter allows one to use methylpheophorbide a nanoparticles as an effective agent for IR-imaging of deeper layers of biotissue. Optical-spectral properties of pheophorbide a and pyropheophorbide in molecular and nano- forms will also be discussed in the report. The results on biodistribution and photodynamic activity of methylpheophorbide a nanoparticles at the cell level and in experimental animals will also be presented. The experiments on the Ehrlich`s carcinoma cell line with the use of laser femtosecond confocal microscopy methods have revealed that non-phototoxic methylpheophorbide a nanoparticles inject phototoxic methylpheophorbide a molecules into the tumor cells when the nanoparticles contact with tumor cell membrane. This makes it possible to fulfil highly selective diagnostics and therapy of oncological diseases.
111 Ytterbium porphyrin complexes and their conjugates with nanoparticles for fluorescent tumour diagnostics A.F. Mironov 1 , V.D. Rumyantseva 1 , A.V. Ivanov 2 , N.G. Hlebtzeov 3 , I.P. Shilov 4 , A.V. Geynits 5 . 1 Moscow State University of Fine Chemical Technologies named after Lomonosov, Moscow, Russia; 2 N.N. Blokhin Cancer Research Center, Moscow, Russia; 3 Saratov State University named after Chernyshevsky, Saratov, Russia; 4 Institute of Radioengineering and Radioelectronics named after Kotelnikov, Moscow, Russia; 5 State Research and Clinical Center for Laser Medicine of Healthcare Ministry of Russia, Moscow, Russia Introduction: One of the perspective directions in early diagnostics of malignant neoplasms which can be revealed endoscopically is fluorescent diagnostics with ytterbium porphyrin complexes. They have an intensive fluorescence in the near IR-spectrum in the so-called “window of tissue transparency”; they accumulate well in tumors, practically do not generate singlet oxygen what is an important advantage comparing to photosensitizers traditionally used for PDT in onologic lesions because ytterbium porphyrin complexes do not cause tissue destruction which is an adverse effect in this case. An effective delivery of ytterbium photosensitizer complexes (Yb-PsC) to a target-tumour is successfully realized due to the formation of conjugates with nanoparticle of definite size.