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Abstracts / Pregnancy Hypertension: An International Journal of Women’s Cardiovascular Health 5 (2015) 53–156
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Cellular and subcellular localization of angiotensin AT1 and AT2 receptors in guinea-pig utero-placental units along pregnancy Stephanie Acuna, Gloria Valdes (Pontificia Universidad Catolica, Santiago, Chile)
Assessment of concentrations of sFlt-1 and PlGF as predictors of preeclampsia Jackeline S. Machado a, Michelle S. Machado a, Tawana V. Bertagnolli a, Geraldo Duarte a, Valéria C. Sandrim b, Ricardo C. Cavalli a (a Ribeirão Preto Medical School – University of Sao Paulo, Ribeirão Preto, Brazil, b UNESP, Botucatu, Brazil)
Objectives: In humans, angiotensin II type 1 and 2 receptors (AT1R and AT2R) have been implicated in trophoblast invasion and placental development. Since the utero-placental unit of guinea-pigs (GP) and humans share a morphologic structure, hormonal, vasodilator patterns and uterine spiral artery remodeling, this species was deemed suitable to study the spatio-temporal expression of the vasoconstrictive (AT1R) and vasodilator (AT2R) receptors of angiotensin II. Methods: Utero-placental units were collected in days (D) 15, 20, 40 and 60 of a 64–67 days long pregnancy in Pirbright White guinea-pigs (n = 3 per period). The localization and intensity of expression of AT1R and AT2R in different structures of utero-placental units was evaluated by immunohistochemistry. Additionally immunogold electron microscopy for both receptors was performed in day 60. Results: AT1R and AT2R were detected along pregnancy in the interlobium, the endothelium and syncytiotrophoblast of the placental labyrinth, subplacenta, giant cells, syncytial streamers, decidua, vascular smooth muscle of spiral arteries and myometrium; in day 20 in perivascular and intramural trophoblasts, while in late pregnancy these were observed in endoluminal trophoblasts in lateral and myometrial spiral arteries. The immunoreactivity of AT1R and AT2R was prominent in D15 in syncytial streamers and in D40 in intramural and intraluminal trophoblasts. Immunoelectron microscopy in D60 demonstrated both receptors in cell membrane, cytosol (cyt) and nucleus (nu) in the placenta (Figure). Conclusions: The spatial pattern of AT1R and AT2R supports their association with decidualization, trophoblast invasion, vascular remodeling. This similarity with the human, added to an analogous intracellular localization of AT1R, validates the use of guinea-pigs to understand the local adaptations of pregnancy. Supported by Fondecyt 1121161.
Objectives: Recent studies have shown that inadequate vascularization of the placenta may be caused by changes in the concentration of anti-angiogenic and pro-angiogenic factors like sFlt-1 and PlGF in maternal circulation indicating that they play an important role in the pathophysiology of preeclampsia. This study aimed evaluate the potential of sFlt-1 and PlGF as predictors of preeclampsia. Methods: We studied 50 healthy pregnant women (HP) and 30 pregnant women with preeclampsia (PE) with a mean gestational age of 23 weeks. The measurements of concentrations of sFlt-1 and PlGF were determined using the ELISA method. Results: When compared the concentrations of sFlt-1 to HP (135.08 pg/ml ± 76.4) and PE (186.55 pg/ml ± 182.4) no statistical difference between groups was observed p = 0.40. The concentrations of PlGF were greater in HP (483.17 pg/ml ± 381.7) compared to PE group (363.97 pg/ ml ± 332.5), p = 0.01. Pulsatility index uterine artery average (right and left) was higher in the PE then in GS (1.05 ± 0.4 vs. 0.86 ± 0.4) p = 0.02 as expected pressure levels in the PE group were higher and the birth weight was lower in the PE group compared with the GS group. Conclusions: Our study showed that despite not having a significant difference, the concentrations of sFlt-1 levels were higher in women from the PE group, the concentrations of PlGF were already lower in the PE group compared the HP, confirming that the variation in concentrations of factors anti-angionic and pro-angiogenic factors are involved in the pathophysiology of preeclampsia. Despite these results ROC curves show that these biomarkers were not effective as predictors of preeclampsia.
Disclosures: J.S. Machado: None. M.S. Machado: None. T.V. Bertagnolli: None. G. Duarte: None. V.C. Sandrim: None. R.C. Cavalli: None. Disclosures: S. Acuna: None. G. Valdes: None. doi:10.1016/j.preghy.2014.10.114 doi:10.1016/j.preghy.2014.10.113
Abstracts / Pregnancy Hypertension: An International Journal of Women’s Cardiovascular Health 5 (2015) 53–156
[107-POS]
[108-POS]
Cellular and subcellular localization of angiotensin AT1 and AT2 receptors in guinea-pig utero-placental units along pregnancy Stephanie Acuna, Gloria Valdes (Pontificia Universidad Catolica, Santiago, Chile)
Assessment of concentrations of sFlt-1 and PlGF as predictors of preeclampsia Jackeline S. Machado a, Michelle S. Machado a, Tawana V. Bertagnolli a, Geraldo Duarte a, Valéria C. Sandrim b, Ricardo C. Cavalli a (a Ribeirão Preto Medical School – University of Sao Paulo, Ribeirão Preto, Brazil, b UNESP, Botucatu, Brazil)
Objectives: In humans, angiotensin II type 1 and 2 receptors (AT1R and AT2R) have been implicated in trophoblast invasion and placental development. Since the utero-placental unit of guinea-pigs (GP) and humans share a morphologic structure, hormonal, vasodilator patterns and uterine spiral artery remodeling, this species was deemed suitable to study the spatio-temporal expression of the vasoconstrictive (AT1R) and vasodilator (AT2R) receptors of angiotensin II. Methods: Utero-placental units were collected in days (D) 15, 20, 40 and 60 of a 64–67 days long pregnancy in Pirbright White guinea-pigs (n = 3 per period). The localization and intensity of expression of AT1R and AT2R in different structures of utero-placental units was evaluated by immunohistochemistry. Additionally immunogold electron microscopy for both receptors was performed in day 60. Results: AT1R and AT2R were detected along pregnancy in the interlobium, the endothelium and syncytiotrophoblast of the placental labyrinth, subplacenta, giant cells, syncytial streamers, decidua, vascular smooth muscle of spiral arteries and myometrium; in day 20 in perivascular and intramural trophoblasts, while in late pregnancy these were observed in endoluminal trophoblasts in lateral and myometrial spiral arteries. The immunoreactivity of AT1R and AT2R was prominent in D15 in syncytial streamers and in D40 in intramural and intraluminal trophoblasts. Immunoelectron microscopy in D60 demonstrated both receptors in cell membrane, cytosol (cyt) and nucleus (nu) in the placenta (Figure). Conclusions: The spatial pattern of AT1R and AT2R supports their association with decidualization, trophoblast invasion, vascular remodeling. This similarity with the human, added to an analogous intracellular localization of AT1R, validates the use of guinea-pigs to understand the local adaptations of pregnancy. Supported by Fondecyt 1121161.
Objectives: Recent studies have shown that inadequate vascularization of the placenta may be caused by changes in the concentration of anti-angiogenic and pro-angiogenic factors like sFlt-1 and PlGF in maternal circulation indicating that they play an important role in the pathophysiology of preeclampsia. This study aimed evaluate the potential of sFlt-1 and PlGF as predictors of preeclampsia. Methods: We studied 50 healthy pregnant women (HP) and 30 pregnant women with preeclampsia (PE) with a mean gestational age of 23 weeks. The measurements of concentrations of sFlt-1 and PlGF were determined using the ELISA method. Results: When compared the concentrations of sFlt-1 to HP (135.08 pg/ml ± 76.4) and PE (186.55 pg/ml ± 182.4) no statistical difference between groups was observed p = 0.40. The concentrations of PlGF were greater in HP (483.17 pg/ml ± 381.7) compared to PE group (363.97 pg/ ml ± 332.5), p = 0.01. Pulsatility index uterine artery average (right and left) was higher in the PE then in GS (1.05 ± 0.4 vs. 0.86 ± 0.4) p = 0.02 as expected pressure levels in the PE group were higher and the birth weight was lower in the PE group compared with the GS group. Conclusions: Our study showed that despite not having a significant difference, the concentrations of sFlt-1 levels were higher in women from the PE group, the concentrations of PlGF were already lower in the PE group compared the HP, confirming that the variation in concentrations of factors anti-angionic and pro-angiogenic factors are involved in the pathophysiology of preeclampsia. Despite these results ROC curves show that these biomarkers were not effective as predictors of preeclampsia.
Disclosures: J.S. Machado: None. M.S. Machado: None. T.V. Bertagnolli: None. G. Duarte: None. V.C. Sandrim: None. R.C. Cavalli: None. Disclosures: S. Acuna: None. G. Valdes: None. doi:10.1016/j.preghy.2014.10.114 doi:10.1016/j.preghy.2014.10.113