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1136 Histological analysis of human glial cells transplanted into rodents
S146
1134 Dept. Japan.
PATTERNS OF MOVEMENTS OF MOUSE FETUSES AT THE EARLIEST DEVELOPMENTAL STAGES OF THE FETAL MOTILITY of Physioi., Sch. of Med., Tokyo Med. Dent. Univ., l-5-45 Yushima, Bunkyo-ku, Tokyo 113,
TOSHIHIKO SUZUE Using the in vitro transplacental perfusion method, we have previously demonstrated that the earliest fetal movements of the mouse occur at around embryonic day 12 (Suzue, 1996). Spontaneous movements of fetuses at this stage were mostly observed only during activity phases that took place once every few minutes. During activity phases, movements of various body regions occurred successively for several seconds. In the present study, detailed analyses on the patterns of the fetal movements, the most primitive forms of fetal behavior, were carried out using a computerized method for the measurement of body movements. Since the movements during activity phases were initiated by the movements of the head, forelimbs, hindlimbs or the tail, the patterns of movements could be classified according to the body regions where the movements were initiated. Detailed analyses revealed that the head-initiated patterns of the movements could be further classified into two subclasses.
1135
NEUROTRANSMITTER RECEPTORS ARE EXPRESSED IN NTPN CELLS DERIVED FROM A TERATOCARCINOMA CELL LINE Department of Neurosurgery’, Physilogy, Kobe University School of Medicine, 7-5-l Kusunoki-cho, Chuoku, Kobe 650, Japan2 TAKESHI KONDOH’, TOMOYUKI NISHIZAK12, MASAHIRO MOR12, YASUHIRO OKADA
NORIHIKO
TAMAKI’,
TOSHIYUKI
MATSUOKA2,
NTZN cells, which are differentiated from human teratocarcima cell line, had the voltage-dependent Na+ channels. In addition, the glutamate receptors such as NMDA and AMPA/kainate receptors and the GABAA receptors were expressed in NTPN cells. We assessed the properties of these receptor channels using whole-cell voltage-clamp and single channel recording.
1136 Department
HISTOLOGICAL ANALYSIS OF HUMAN GLIAL RODENTS of Neurosurgery, Kobe University School of Medicine
HIDEYUKI
AKIYAMA,
TAKESHI
KONDOH,
TAKASHI
KOKUNAI,
CELLS TRANSPLANTED
NORIHIKO
INTO
TAMAKI
Normal human white matter tissues obtained at the time of craniotomies were cultured and 5~1 of the cell suspension(1 x lo6 cells/ml) of those was transplanted into the striatum of wistar rats. Following the transplantation, lOmg/kg of Cycrosporin was injected for 2 weeks. Three or 8 weeks later all rats were sacrificed for histological analysis. Grafted sections were stained with human GFAP and the migration of the human glial cells following transplantation was investigated.
1134 Dept. Japan.
PATTERNS OF MOVEMENTS OF MOUSE FETUSES AT THE EARLIEST DEVELOPMENTAL STAGES OF THE FETAL MOTILITY of Physioi., Sch. of Med., Tokyo Med. Dent. Univ., l-5-45 Yushima, Bunkyo-ku, Tokyo 113,
TOSHIHIKO SUZUE Using the in vitro transplacental perfusion method, we have previously demonstrated that the earliest fetal movements of the mouse occur at around embryonic day 12 (Suzue, 1996). Spontaneous movements of fetuses at this stage were mostly observed only during activity phases that took place once every few minutes. During activity phases, movements of various body regions occurred successively for several seconds. In the present study, detailed analyses on the patterns of the fetal movements, the most primitive forms of fetal behavior, were carried out using a computerized method for the measurement of body movements. Since the movements during activity phases were initiated by the movements of the head, forelimbs, hindlimbs or the tail, the patterns of movements could be classified according to the body regions where the movements were initiated. Detailed analyses revealed that the head-initiated patterns of the movements could be further classified into two subclasses.
1135
NEUROTRANSMITTER RECEPTORS ARE EXPRESSED IN NTPN CELLS DERIVED FROM A TERATOCARCINOMA CELL LINE Department of Neurosurgery’, Physilogy, Kobe University School of Medicine, 7-5-l Kusunoki-cho, Chuoku, Kobe 650, Japan2 TAKESHI KONDOH’, TOMOYUKI NISHIZAK12, MASAHIRO MOR12, YASUHIRO OKADA
NORIHIKO
TAMAKI’,
TOSHIYUKI
MATSUOKA2,
NTZN cells, which are differentiated from human teratocarcima cell line, had the voltage-dependent Na+ channels. In addition, the glutamate receptors such as NMDA and AMPA/kainate receptors and the GABAA receptors were expressed in NTPN cells. We assessed the properties of these receptor channels using whole-cell voltage-clamp and single channel recording.
1136 Department
HISTOLOGICAL ANALYSIS OF HUMAN GLIAL RODENTS of Neurosurgery, Kobe University School of Medicine
HIDEYUKI
AKIYAMA,
TAKESHI
KONDOH,
TAKASHI
KOKUNAI,
CELLS TRANSPLANTED
NORIHIKO
INTO
TAMAKI
Normal human white matter tissues obtained at the time of craniotomies were cultured and 5~1 of the cell suspension(1 x lo6 cells/ml) of those was transplanted into the striatum of wistar rats. Following the transplantation, lOmg/kg of Cycrosporin was injected for 2 weeks. Three or 8 weeks later all rats were sacrificed for histological analysis. Grafted sections were stained with human GFAP and the migration of the human glial cells following transplantation was investigated.