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131. Circulating immune complexes during immunotherapy
American
A6ademy
of Allergy
J. ALLERGY CLIN. IMMlJNOL. MARCH 3978
control subjects. The averaged percentile rankings of the 6to 72-hr responses of 26 patients with reticuloendothelial system neoplasms were significantly less than those of control subjects. The difference in responsiveness between patients with carcinoma and reticuloendothelial tumors at 6 hr was statistically significant (p < 0.05). Hyporesponsiveness (
ersistence of marrow immunosuppressive
lymphocytes therapy.
buck, M.D., §. G. Dienst, M.D., J. A Anderson, M.D., and R. MacDonald, ich.
in
Detroit,
FIeretofore, assessment of clinical immunosuppressive therapy has been quantifying the number of peripheral blood lymphocytes, especially the circulating T lymphocyte population. In patients after organ transplantation but with concurrent immunosuppressive agents administered, gradual rejection of the transplanted organ has occurred with practical ablation of circulating T lymphocytes. We observed an unexpected persistence of marrow lymphocytes in 22 patients of this type in contrast to low circulating lymphocyte counts in many. Lymphocytes constitute 5.2% to 21% of the nucleated celis of normal adult human marrow (or 50,000 to 100,000 lymphocytes per mm3). In experimental animals, in which absolute counts are possible, for every blood lympbocyte there are 20 marrow lymphocytes. Our finding of persisting marrow lymphocytes of 17,780 to 405,000 per mm3 (average, 45,000) in 37 transplant studies with immunosuppresive therapy draws attention to the importance of marrow lymphocyte storage as an area which may escape suppressants. Our findings in such a heavily immunosuppressed group suggest the propriety of assessing such marrow lymphocyte escape as an adjunct to more moderate clinical immunosuppressive therapy utilized in other patient groups.
rcuiating otherapy.
immune
complexes
during
N. J. Gilmore, Ph.D., M.D., W. Ii. Yang, M.D., and G. Dorval, M.D., Ph.D., ontrka!, Quebec, Canada. The possibility that repeated immunization may lead to the production of immune complexes (IC) has been examined by assaying sera of allergic adults undergoing immunotherapy Heat-decomplemented sera from 1 IO consecutive outpatients were reacted with Fc receptor-bearing cells (“modified Raji” cells selected for a high density of Fc receptors), the cells washed, and adsorbed IgG IC quantitated by fixation of lzsI-protein A. IC were expressed as fig/ml heat-aggregated human IgG. Forty-five sera assayed
in parallel with L-1210 mmine tumor cells showed identical results. Thirty-six individuals were assayed prior to immunotherapy and 76 after at least I yr of therapy. Sera from 64 healthy nonallergic adults (2.8 pg/ml k 3.8 SD) contained <20 pg/ml XC. Sera containing <20 pg/ml have been considered negative here. Data were analyzed by the Student’s t test (Table I), TABLE
I
Sera
Number (%I
Age hurl
Sex (MEI
Treated Positive
74 17 (23)
50 47
19155 3/14
Untreated Positive
36 2 (5)
32 33
9127 l/l
Duration hwl
&g/m!
2.7 2.0 -
362 117 il0 iI 154 7? 69 !I
17 43
The IC concentration of the untreated group did not differ significantly from healthy control subjects; treated individuals, as a group, did not differ from healthy control subjects or the untreated group, but 23% had increased IC concentrations compared with only 5% of the untreated group. These data suggest immunotherapy is not uniformly associated with the development of IC but that a subset of individuals may be predisposed to produce IC during immunotherapy
132. E rosette formation in skin test-reactive asthmatic patients, skin test-nonreactive asthmatic patients normal control subjects. M. C. Louie, D. F. German, M.D., FAA., V. Byers, Ph. L. LeCam, Ph.D., San Francisco, Calif. It has been reported that there is a T Lymphocyte deficiency in unclassified asthmatic patients. This study was designed to compare both active E rosette formation and total E rosette formation not only between asthmatic and normal control subjects but also to determine if there is a difference in rosette formation between asthmatic patients with strong skin test reactivity to inhalant antigens and asthmatic patients with minimal or no skin test reactivity. We selected 15 patients, 25 to 56 yr of age, who had documented asthma without skin test reactivity to inhalant antigens. Comparisons were made with 25 age- and sexmatched asthmatic patients with strong skin test reactivity and 25 healthy nonallergic, nonasthmatic age- and sexmatched control subjects. All asthmatic subjects, except one, were wheeze free for greater than 1 mo and had no other illnesses or systemic drug therapy *hat could alter the lymphocyte counts. We found that the three populations did not differ in absolute numbers of active and total E rosettes? nor did they differ in the percent of total circulating lymphocytes that were T cells. But the skin test-reactive asth-
A6ademy
of Allergy
J. ALLERGY CLIN. IMMlJNOL. MARCH 3978
control subjects. The averaged percentile rankings of the 6to 72-hr responses of 26 patients with reticuloendothelial system neoplasms were significantly less than those of control subjects. The difference in responsiveness between patients with carcinoma and reticuloendothelial tumors at 6 hr was statistically significant (p < 0.05). Hyporesponsiveness (
ersistence of marrow immunosuppressive
lymphocytes therapy.
buck, M.D., §. G. Dienst, M.D., J. A Anderson, M.D., and R. MacDonald, ich.
in
Detroit,
FIeretofore, assessment of clinical immunosuppressive therapy has been quantifying the number of peripheral blood lymphocytes, especially the circulating T lymphocyte population. In patients after organ transplantation but with concurrent immunosuppressive agents administered, gradual rejection of the transplanted organ has occurred with practical ablation of circulating T lymphocytes. We observed an unexpected persistence of marrow lymphocytes in 22 patients of this type in contrast to low circulating lymphocyte counts in many. Lymphocytes constitute 5.2% to 21% of the nucleated celis of normal adult human marrow (or 50,000 to 100,000 lymphocytes per mm3). In experimental animals, in which absolute counts are possible, for every blood lympbocyte there are 20 marrow lymphocytes. Our finding of persisting marrow lymphocytes of 17,780 to 405,000 per mm3 (average, 45,000) in 37 transplant studies with immunosuppresive therapy draws attention to the importance of marrow lymphocyte storage as an area which may escape suppressants. Our findings in such a heavily immunosuppressed group suggest the propriety of assessing such marrow lymphocyte escape as an adjunct to more moderate clinical immunosuppressive therapy utilized in other patient groups.
rcuiating otherapy.
immune
complexes
during
N. J. Gilmore, Ph.D., M.D., W. Ii. Yang, M.D., and G. Dorval, M.D., Ph.D., ontrka!, Quebec, Canada. The possibility that repeated immunization may lead to the production of immune complexes (IC) has been examined by assaying sera of allergic adults undergoing immunotherapy Heat-decomplemented sera from 1 IO consecutive outpatients were reacted with Fc receptor-bearing cells (“modified Raji” cells selected for a high density of Fc receptors), the cells washed, and adsorbed IgG IC quantitated by fixation of lzsI-protein A. IC were expressed as fig/ml heat-aggregated human IgG. Forty-five sera assayed
in parallel with L-1210 mmine tumor cells showed identical results. Thirty-six individuals were assayed prior to immunotherapy and 76 after at least I yr of therapy. Sera from 64 healthy nonallergic adults (2.8 pg/ml k 3.8 SD) contained <20 pg/ml XC. Sera containing <20 pg/ml have been considered negative here. Data were analyzed by the Student’s t test (Table I), TABLE
I
Sera
Number (%I
Age hurl
Sex (MEI
Treated Positive
74 17 (23)
50 47
19155 3/14
Untreated Positive
36 2 (5)
32 33
9127 l/l
Duration hwl
&g/m!
2.7 2.0 -
362 117 il0 iI 154 7? 69 !I
17 43
The IC concentration of the untreated group did not differ significantly from healthy control subjects; treated individuals, as a group, did not differ from healthy control subjects or the untreated group, but 23% had increased IC concentrations compared with only 5% of the untreated group. These data suggest immunotherapy is not uniformly associated with the development of IC but that a subset of individuals may be predisposed to produce IC during immunotherapy
132. E rosette formation in skin test-reactive asthmatic patients, skin test-nonreactive asthmatic patients normal control subjects. M. C. Louie, D. F. German, M.D., FAA., V. Byers, Ph. L. LeCam, Ph.D., San Francisco, Calif. It has been reported that there is a T Lymphocyte deficiency in unclassified asthmatic patients. This study was designed to compare both active E rosette formation and total E rosette formation not only between asthmatic and normal control subjects but also to determine if there is a difference in rosette formation between asthmatic patients with strong skin test reactivity to inhalant antigens and asthmatic patients with minimal or no skin test reactivity. We selected 15 patients, 25 to 56 yr of age, who had documented asthma without skin test reactivity to inhalant antigens. Comparisons were made with 25 age- and sexmatched asthmatic patients with strong skin test reactivity and 25 healthy nonallergic, nonasthmatic age- and sexmatched control subjects. All asthmatic subjects, except one, were wheeze free for greater than 1 mo and had no other illnesses or systemic drug therapy *hat could alter the lymphocyte counts. We found that the three populations did not differ in absolute numbers of active and total E rosettes? nor did they differ in the percent of total circulating lymphocytes that were T cells. But the skin test-reactive asth-