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150 Food allergy in atopic dogs
176
149
Abstracts
l!EiLLEmAA
*. M.
cRntani.RphD.,
nD*
.
I!,&oGB
W.
s
Italy
With the moperation of 48 Maternity Hospitals we have started a prospective study to evaluate the effect of dietary ard envirommtal measures in the developnt of atopio disease in “at risk” newborns ‘The preventive ma-ares included dietary (exclusive breast feeding for the first 6 mnths of life; eliminat;on of cow’s mrlk (Cn) and egg frm the diet of the nursing mther, soy milk (Isanil Abbott) supplenmt when breast mlk is not sufficient, seleotsd weaning after the 6th mnth of life) and environuental measures (no smkiq and no pets in the house; elimination of house dust; day-care center attembnce delayed to after 3 years). All infants were seen a.t the
age of
1,
3,
6,
9,
12 months
and
twxe-a-year
afterwards. 2291 babies (1167 Il. 1124 F) have haen e?zolled The results were statistically svaluated with the X2 mthod. 2141829 kabies were exolusively ?zresst-fed until the 6th mnth of life, 1231829 recelved hreas: milk ard soy-tilk supplemnt, 156/829 were QI fomla-fed. bring the 12-rmnths follow-up 53/347 (15%) developed atoplo symptm’ 42 atopm derrmtitis, 5 asthm, amzI 6 food allergy. AZ regards the typ of feeding, 111214 (5%) were exolusively beast-fed, lo/193 (5%) received exclusively soy milk, 111266 (4%) breast mlk aml soy-milk supplement, ard 211156 (13%) CM The very low premlence of atopio Asease in the infants who followed the preventive meamres (321673 = 5%) and the higher (211156 = 13%) in those who &d not (0.013 = p = 0.0001). stresses the importance of such mprlat~onz for the Fevention of atom in “at risk” babies
Theprevalemxof fatal tinear-fatal foaj anaphylacticx+acticminchildmnisunknom In6llKmths, 4 CUldcaseS~rarelYreported. casgoffatal fcxxlatw@ylaxisand 4rsearfatalcaseshavebeenreviewed. Pts.rangedin age fmn2-17years;2inalesard6 females. All pts.wereatcpicardon~forast4nm. Allpts.hadexperiemedprevicusreactirmstc the inu&ina~food(pearnrt-4, filbert:; egg-l,milk-l,walraR-l),Wonly~e prwiclr;ly experiemed a life threaIn all cases,thechildiqesbAthe ticm.
allergenunknahqlyandaway frunhaw. Adm3k3linwasmtadministeredearly inany fatal case ti was not available in 3/4 caees. ?he kinetics of the rextims were variable. In fatal cases, 5qmptcm were first noted lo-20 2-hadasteady min. post-iqesticn; prcqression of -while 2 had a quiescent period ( 2 hr) before fatal respiratory sump tar6 developed. In non-fatal cases, first sqptuns were noted in l-5 mins. and in 31’4 cases, adrenalin was given within 15-30 mins of ort=et of onset. respiratory syrrptapm
leadirq
arrest follaJed a 55-125 in 3/4 m-fatal pts, 2 had received adrwlalin early. In 7/8 fatal or near-fatal of fuod anaphylaxis in knmm food allergic patients, adrenalinwas not readily available and in no case did pts. or parents appreciate the lifethreatening potential of their food allergy. min.
FOOD ALLERGY 11 ATOPIC DOGS. Oscar L. Frick,.XD & I:. Grant Guilford,ACVIM. San Francisco & Davis,CA -.--_ Food allcrav -, research has been hammered bv lack of suitable animal models. The inbred atopic dog has been useful for studies on respiratory allergy mechanisms and pharmacologic manipulations. We apPlied a sensitization regimen for food allergens to two litters of pups born to atopic dogs. At birth, half the PUPS in each litter of 9 and g were injected SC with 1 ,ug cow’s milk or soy &tigen in 0.2~11 alum. At 3,7. and 11 weeks, (111 pups were injected SC with 0.5ml live,attcnuated hepatitis,distemper vaccine.followed in 2 and 9 days by either lpg soy or cow’s milk in 0.2ml alum,as they received as newborns. After weaning,all pups were fed standard Puppy Chow that contained meat,milk, soy, wheat,corn,no fish or potato. At B-4 mo, all pups were making canine IgE-RAST antibodies to several foods in diet,but significantly higher titers to cow’s milk or soy in their original scnsitization. At 4 -5 mo,for 4 days,number and quality of stools/day were noted. Then blinded, they were fed either 240 ml cow’s milk or soy and observed closely for next week with daily stool rounts and for other signs. There was if significant correlation between increased number stools after specific challenge with IgE anti-food level. At 6 -7 mo, 4 food-sensitized, 2 ragweed only and 2 non-atopic mongrel pups were endoaLOpiC, scoped and 6 food allergens were applied togastric mucosa. After 5 min in sensitive pups, urythema, were observed nt sites tested edema, and petechiae reactions to with cow’s milk or soy, and lesser and negative to fish nnd potato. wheat and corn, which were absent from diet. Thus, by immunologic and clinical observations, the atopic dog appears to be an appropriate model for food allergy studies.
tc respiratory
cpi escentperiod
E?ette.r ---
150
reac-
statistics
and
better&ucatio~l iJlthiSare;l.
152-C-lDAA Baltisroe,
lsl3zyliurl
a r&uxal ccxalrrirg plant psyllim mlrcilloid, fiber, is an active itgxdient inkulklaxative prqaraticnsardmmntlykisheenshmnto have c+x~le5teml-lcmring effects. Allergic reactionstopiylli~&sthavabeen~ibed inp4lmmmticalandhsalulcaz7?-,~ rarely follcwiq its bqestirn. several claims of anaphylactic reacticm follwirq the bgescereals have M tion of psyllimn-amtainimj reported. Historical data was abtati fm 19/24 mrenreportirqallex~ic rwcthas to this ceraal batueen Sept. '89 and Ruxh '90. Probein fracticms fm the cereal, pqllium xmxilloid, inirqlaxative, were and a psyllim-anh extra&&quantitated,~aep24ra~bySc6PKE. Specific IgEarri IgGantibaCes tot&se inpatient sexa antigenfracticm3wared&e&ed byinmmcblctting~ques. Nineteen wcrmen(13 rauses) wz?x evaluated: 17 (89%) had historical aml/or laboratory evideme of atcgy; 16 (84%) had s or dispmxdp6ylli~iningproducts;and only3 (16%)dmiedanyprevials~. synptonrs
otxxrmd
within
3-30
mim
an3 ucst
axmKmlyincludedwheezingand~thraat arrlchestti~, afxiurticaria. Allbxmen I-equhdtical therapy, 11(58%) in an ER. specific IgE ard I* antibodies to psyllim inallsubjf&s:cmly fracticut4uere~ in 5 antrols. Iqzantibcdi~wereImiividualswithoccq&icmlexposuretc psyllimdust are at hi* riskof reactim~ tc -. igestedpsylli~iningpW
149
Abstracts
l!EiLLEmAA
*. M.
cRntani.RphD.,
nD*
.
I!,&oGB
W.
s
Italy
With the moperation of 48 Maternity Hospitals we have started a prospective study to evaluate the effect of dietary ard envirommtal measures in the developnt of atopio disease in “at risk” newborns ‘The preventive ma-ares included dietary (exclusive breast feeding for the first 6 mnths of life; eliminat;on of cow’s mrlk (Cn) and egg frm the diet of the nursing mther, soy milk (Isanil Abbott) supplenmt when breast mlk is not sufficient, seleotsd weaning after the 6th mnth of life) and environuental measures (no smkiq and no pets in the house; elimination of house dust; day-care center attembnce delayed to after 3 years). All infants were seen a.t the
age of
1,
3,
6,
9,
12 months
and
twxe-a-year
afterwards. 2291 babies (1167 Il. 1124 F) have haen e?zolled The results were statistically svaluated with the X2 mthod. 2141829 kabies were exolusively ?zresst-fed until the 6th mnth of life, 1231829 recelved hreas: milk ard soy-tilk supplemnt, 156/829 were QI fomla-fed. bring the 12-rmnths follow-up 53/347 (15%) developed atoplo symptm’ 42 atopm derrmtitis, 5 asthm, amzI 6 food allergy. AZ regards the typ of feeding, 111214 (5%) were exolusively beast-fed, lo/193 (5%) received exclusively soy milk, 111266 (4%) breast mlk aml soy-milk supplement, ard 211156 (13%) CM The very low premlence of atopio Asease in the infants who followed the preventive meamres (321673 = 5%) and the higher (211156 = 13%) in those who &d not (0.013 = p = 0.0001). stresses the importance of such mprlat~onz for the Fevention of atom in “at risk” babies
Theprevalemxof fatal tinear-fatal foaj anaphylacticx+acticminchildmnisunknom In6llKmths, 4 CUldcaseS~rarelYreported. casgoffatal fcxxlatw@ylaxisand 4rsearfatalcaseshavebeenreviewed. Pts.rangedin age fmn2-17years;2inalesard6 females. All pts.wereatcpicardon~forast4nm. Allpts.hadexperiemedprevicusreactirmstc the inu&ina~food(pearnrt-4, filbert:; egg-l,milk-l,walraR-l),Wonly~e prwiclr;ly experiemed a life threaIn all cases,thechildiqesbAthe ticm.
allergenunknahqlyandaway frunhaw. Adm3k3linwasmtadministeredearly inany fatal case ti was not available in 3/4 caees. ?he kinetics of the rextims were variable. In fatal cases, 5qmptcm were first noted lo-20 2-hadasteady min. post-iqesticn; prcqression of -while 2 had a quiescent period ( 2 hr) before fatal respiratory sump tar6 developed. In non-fatal cases, first sqptuns were noted in l-5 mins. and in 31’4 cases, adrenalin was given within 15-30 mins of ort=et of onset. respiratory syrrptapm
leadirq
arrest follaJed a 55-125 in 3/4 m-fatal pts, 2 had received adrwlalin early. In 7/8 fatal or near-fatal of fuod anaphylaxis in knmm food allergic patients, adrenalinwas not readily available and in no case did pts. or parents appreciate the lifethreatening potential of their food allergy. min.
FOOD ALLERGY 11 ATOPIC DOGS. Oscar L. Frick,.XD & I:. Grant Guilford,ACVIM. San Francisco & Davis,CA -.--_ Food allcrav -, research has been hammered bv lack of suitable animal models. The inbred atopic dog has been useful for studies on respiratory allergy mechanisms and pharmacologic manipulations. We apPlied a sensitization regimen for food allergens to two litters of pups born to atopic dogs. At birth, half the PUPS in each litter of 9 and g were injected SC with 1 ,ug cow’s milk or soy &tigen in 0.2~11 alum. At 3,7. and 11 weeks, (111 pups were injected SC with 0.5ml live,attcnuated hepatitis,distemper vaccine.followed in 2 and 9 days by either lpg soy or cow’s milk in 0.2ml alum,as they received as newborns. After weaning,all pups were fed standard Puppy Chow that contained meat,milk, soy, wheat,corn,no fish or potato. At B-4 mo, all pups were making canine IgE-RAST antibodies to several foods in diet,but significantly higher titers to cow’s milk or soy in their original scnsitization. At 4 -5 mo,for 4 days,number and quality of stools/day were noted. Then blinded, they were fed either 240 ml cow’s milk or soy and observed closely for next week with daily stool rounts and for other signs. There was if significant correlation between increased number stools after specific challenge with IgE anti-food level. At 6 -7 mo, 4 food-sensitized, 2 ragweed only and 2 non-atopic mongrel pups were endoaLOpiC, scoped and 6 food allergens were applied togastric mucosa. After 5 min in sensitive pups, urythema, were observed nt sites tested edema, and petechiae reactions to with cow’s milk or soy, and lesser and negative to fish nnd potato. wheat and corn, which were absent from diet. Thus, by immunologic and clinical observations, the atopic dog appears to be an appropriate model for food allergy studies.
tc respiratory
cpi escentperiod
E?ette.r ---
150
reac-
statistics
and
better&ucatio~l iJlthiSare;l.
152-C-lDAA Baltisroe,
lsl3zyliurl
a r&uxal ccxalrrirg plant psyllim mlrcilloid, fiber, is an active itgxdient inkulklaxative prqaraticnsardmmntlykisheenshmnto have c+x~le5teml-lcmring effects. Allergic reactionstopiylli~&sthavabeen~ibed inp4lmmmticalandhsalulcaz7?-,~ rarely follcwiq its bqestirn. several claims of anaphylactic reacticm follwirq the bgescereals have M tion of psyllimn-amtainimj reported. Historical data was abtati fm 19/24 mrenreportirqallex~ic rwcthas to this ceraal batueen Sept. '89 and Ruxh '90. Probein fracticms fm the cereal, pqllium xmxilloid, inirqlaxative, were and a psyllim-anh extra&&quantitated,~aep24ra~bySc6PKE. Specific IgEarri IgGantibaCes tot&se inpatient sexa antigenfracticm3wared&e&ed byinmmcblctting~ques. Nineteen wcrmen(13 rauses) wz?x evaluated: 17 (89%) had historical aml/or laboratory evideme of atcgy; 16 (84%) had s or dispmxdp6ylli~iningproducts;and only3 (16%)dmiedanyprevials~. synptonrs
otxxrmd
within
3-30
mim
an3 ucst
axmKmlyincludedwheezingand~thraat arrlchestti~, afxiurticaria. Allbxmen I-equhdtical therapy, 11(58%) in an ER. specific IgE ard I* antibodies to psyllim inallsubjf&s:cmly fracticut4uere~ in 5 antrols. Iqzantibcdi~wereImiividualswithoccq&icmlexposuretc psyllimdust are at hi* riskof reactim~ tc -. igestedpsylli~iningpW