[172-POS]

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Abstracts / Pregnancy Hypertension: An International Journal of Women’s Cardiovascular Health 5 (2015) 53–156

Conclusions: The present study shows that the potential markers of lipotoxicity are related to preeclampsia, and eventually may indicate likely prognostics associated with clinical expressions. Additional studies can clarify this correlation and these results might be useful to orientate the adoption of practices which are able to decrease maternal/ fetal risks. Disclosures: B. Zeiger: None. A. Bergamo: None. D. Vidal: None. F. Aires: None. D. Ferreira: None. M. Scarpelini: None. J. Garcia: None. H. Korkes: None. M. Paltronieri: None. S. de Toledo: None. W. Chaiwangyen: None. F. Sousa: None. N. Sass: None. doi:10.1016/j.preghy.2014.10.176

[171-POS] Establishing trimester-specific maternal thyroid hormone reference intervals of southern Chinese population Lei Liu, Xuezhen Zhang, JinYing Yang, Xueya Qian, Zheng Zheng, Xuwen Tang, Huishu Liu (Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou, China) Objectives: Subclinical thyroid disorders are common in pregnant women, which are associated with adverse pregnancy outcomes. Physiological changes in pregnancy and the lack of pregnancy-specific reference ranges pose great challenge for the managements of subclinical thyroid disorders in pregnancy. We aimed to establish trimester-specific thyroid hormone reference intervals throughout pregnancy in southern Chinese population. Methods: we measured the serum thyroid hormone (TSH, FT4, TPO-AB) using Abbott electrochemiluminescence immunoassay in 5589 pregnant women during 2012–2013. Patients with known thyroid disorders, autoimmune disease, recurrent miscarriage, hyperemesis gravidarum and pre-eclampsia were excluded. Trimester-specific reference ranges (2.5th, 97.5th centiles) were calculated. Results: TSH median T1: 1.19 ± 0.99 mIU/L (N = 3532), T2: 1.40 ± 1.02 mIU/L (N = 1623), T3: 1.48 ± 1.30 mIU/L (N = 434). FT4 median T1: 15.7 ± 3.12 pmol/L (N = 3532), T2: 13.81 ± 2.99 pmol/L (N = 1623), T3: 12.92 ± 2.98 pmol/L (N = 434). TSH reference range T1: 0.01–3.55 mIU/L, T2: 0.01–4.13 mIU/L, T3: 0–4.67 mIU/L. FT4 reference range T1: 12–21.44pmol/L, T2: 10.01–19.36 pmol/L, T3: 9.29– 20.31 pmol/L. TSH slightly increased throughout gestation. FT4 decreased throughout gestation. Conclusions: We established pregnancy-specific thyroid function reference intervals of southern Chinese population, which is beneficial in clinical practice. Disclosures: L. Liu: None. X. Zhang: None. J. Yang: None. X. Qian: None. Z. Zheng: None. X. Tang: None. H. Liu: None. doi:10.1016/j.preghy.2014.10.177

[172-POS] Comparative expression profiling of endoplasmic reticulum aminopeptidase 2 and human leukocyte antigen-C expression in choriocarcinoma cell lines Eun D. Lee a, Samone Brockett a, DaShaunda Hilliard b, Maria E. Teves a, Ronald Ramus a, Jerome F. Strauss III a (a Virginia Commonwealth University, Richmond, VA, USA, b Eastern Virginia Medical School, Norfolk, VA, USA) Objectives: Proper regulation of human leukocyte antigenC (HLA-C) expression on trophoblast cells is essential for the immunological privilege of these cells. Endoplasmic reticulum aminopeptidase 2 (ERAP2) is known to trim antigenic peptide precursors for loading onto HLA. Altered expression of the ERAP2 could play a critical role in shaping HLA-C expression and antigen presentation, but their relationships have not been defined. Methods: We examined the genotype and expression levels of ERAP2 and HLA-C in several choriocarcinoma cell lines (BeWo, JAr, and JEG-3) by allelic discrimination qPCR, Western blotting and immunocytochemistry. Additionally, expression profiling of untreated versus gamma-interferon (IFN-c, 20 ng/ml)-treated cells was performed. Lastly, to determine if there is a relationship between ERAP2 and HLA-C expression levels, transfection of major and minor allele ERAP2 variants was performed. Results: JAr cells were homozygous for the rs2248374 minor A allele, and express ERAP2 protein. BeWo and JEG3 cells are homozygous for ERAP2 genetic variant (major G allele of SNP rs2248374) that causes non-sense mediated RNA decay, and thus lack ERAP2 protein expression. Interestingly, BeWo and JEG-3 cells express HLA-C, whereas, JAr cells do not. IFN-c treatment of BeWo and JEG-3 cells increased HLA-C expression by 2-fold (p = 0.0037) and 3-fold (p = 0.0446) above control levels, respectively, but ERAP2 remained undetectable. In contrast, JAr cells treated with IFN-c had increased ERAP2 protein expression by 2-fold (p = 0.003), but had no effect on HLA-C expression. Transfection of JEG-3 cells (ERAP2 null) with ERAP2 plasmids with the SNP rs2549782, encoding an amino acid substitution (N392K) that alters substrate specificities and antigen presentation, revealed that the different ERAP2 isoforms do not affect the expression level of HLA-C. Conclusions: Our observations suggest that the expression patterns of ERAP2 and HLA-C allow choriocarcinoma cells to escape immune surveillance. Disclosures: E.D. Lee: None. S. Brockett: None. D. Hilliard: None. M.E. Teves: None. R. Ramus: None. J.F. Strauss: None. doi:10.1016/j.preghy.2014.10.178

[173-POS] The role of placenta growth factor as predictor of preeclampsia