Poster Presentation Abstracts
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significantly at the PM milking following Na treatment, but protein and lactose content both decreased (protein: 3.69 VS. 3.33 + 0.03%; lactose: 4.57 vs. 4.37 + 0.05%; P < 0.001). In conclusion, elevated Na in milk transiently decreased milk synthesis, suggesting that this may be a potential cause of the milk loss associated with leaky TJ.
18. Response of blood metabolites, body condition, and milk yield of goats to supplementary feeding of different concentrate mixtures. E.R.K. Perera* and A.N.F. Perera. Faculty of Agriculture, University of Peradeniya, Sri Lanka. The response of blood metabolites, body condition, and milk yield of lactating Jamnapari Indigenous (JI) does to supplementary feeding of different concentrate mixtures was examined to select a mixture which improves body condition and milk yield. Twenty does were divided into four groups, balanced by age (3.7 + 0.7 yr), body weight (3 1.7 * 1.7 kg) and days in lactation (9 + 2 days). Kids were separated at birth, and does were stall-fed. Panicum maximum was offered ad libitum to all groups. Three groups were fed (at 2.50 g/doe) with one of three supplementary concentrate mixtures, which contained 30% rice bran and 15% maize meal, but differed in coconut meal (50%, 40%, and 30%) and fish meal (O%, lo%, and 20%) contents. Does were milked twice daily. Measurements were obtained on proximate composition of feeds, group feed intake, milk yield, milk composition, individual body weight, body condition score, heart girth, rumen pH, blood urea nitrogen (BUN), and 17-beta-hydroxybutyrate (BHB) of the does during 60 days. Data were subjected to analysis of variance. Concentrate supplementation improved (P 2 0.05) the intake of dry matter and nitrogen. In response to concentrate supplementation, circulating BHB declined suggesting improvement in energy status, while milk yield increased by 75 to 100%. Substitution of coconut meal with fish meal exerted no extra improvement in body condition or milk yield. Results suggest that supplementary feeding of either concentrate mixture at 250 g/doe day -I improved energy, protein status and milk yield in JI does under local conditions, but inclusion of fish meal in the mixture offered no extra benefits. Of the three mixtures tested, the one with 0% fish meal was less expensive, but superior in improving milk yield, hence, more suitable to be used as a supplementary feed for lactating JI does in Sri Lanka. 19. Chemical composition of milk in sows fed diets supplemented with peanut oil. W. Migdal * and J. Pys. Agricultural University, Krakow, Poland. Observations were carried out on a total of 30 Polish Landrace sows divided into two groups: (I) fed control diets; (II) fed an additional 10% peanut oil supplement from 90 days of pregnancy to 21 days of lactation. After injection of 20 i.u. oxytocin, colostrum was collected 1 and 24 h following placenta expulsion and milk was collected at 7, 14, and 21 days of lactation, 6 h after feeding. Colostrum and milk of sows receiving a supplement of peanut oil was characterised by significantly higher content of fat and energy values. Milk fat of group II sows was characterised by significantly higher content of unsaturated fatty acids - mostly linoleic acid C,,:, - (1 h, 18.4% for group I vs. 20.6% for group II; 7 days, 11.2% vs. 14.9%; 21 days, 10.5% vs. 12.4%) and oleic acid C ,8:, (1 h, 38.1% vs. 39.9%; 21 days, 32.2% vs. 37.5%). The higher fat level and energy value of sow colostrum and milk contributed to better results at weaning. Peanut oil could be used as an energy source during late pregnancy and lactation in sows. 20. Effect of calcium on mammary epithelial cell proliferation and the plasminogen activator system. F. Cheli’ *, B. Zavizion* and I. Politis2. ‘Animal Nu trition Institute, Veterinary Medicine, Milan, Italy and ‘Dept. of Animal and Food Sciences, University of Vermont, Burlington, USA. The objective of this study was to examine: (1) the effect of extracellular calcium on proliferation of bovine mammary epithelial cells (BME-UV) and the plasminogen activator (PA) system; and (2) whether the mitogenic effect of insulin-like growth factor (IGF-I) and epidermal growth factor (EGF) is regulated by extracellular calcium. BME-UV cells were established from primary bovine epithelial cells by stable transfection with a plasmid, carrying the sequence of the simian virus 40 early region mutant tsA58, encoding the thermolabile large T-antigen. Cells were grown in DMEM-F12 medium without Caf+ and Mg++, supplemented with