1H NMR sequential assignments of oxidized ferredoxins from Clostridium pasteurianum and Clostridium acidurici

INTBRNATIONALWORKSHOPONIRON-SULFURPROTBINS:POSTERS17

P14

1H NMR Sequential Assignments of Oxidized Ferredoxins from CLOSTRIDIUM and CLOSTRIDIUM

PASTEURIANUM

ACIDURICI

Jacques Gaillard*, Jean-Marc Moulis#, Rainer Kiimmerle* and Jacques Meyerb, CEA, Departement de Recherche sur la Matiere Condensee, SESAM-SCPM (*) and Departement de Biologie Moleculaire et Structurale, Laboratoire des Metalloproteines (#) CENG, 17 Rue des Martyrs, 38054 GRENOBLE CEDEX, France The two 2[4Fe-4S] ferredoxins from C. pasteurianum have

and C. acidurici NMR.

Using

experiments,

been

investigated

conventional sequential

TOCSY

assignments

by 'H 2D

and NOESY were obtained

for the two segments at the N- and C-termini

and

for the segment between residues 23 and 36. Taking advantage

of

sequence

homology

between

the

two

proteins,

further assignments have been made to a

total of about 80% of the sequence. The

presence

of

the

two

paramagnetic

centers strongly influences the relaxation properties of the neighbouring protons. Nevertheless, some long range NCE correlations analysis,

structural

were displayed.

informations

were

From their extracted:

the contact between the two terminal parts of the proteins

is a highly

conserved

feature

but

small

differences were evidenced between the two proteins and

are

to

be

related to variations observed between some properties of these ferredoxins. J. Gaillard, Magnetic

J.-M. Moulis,

Resonance

J. Meyer, R. Kiimmerle,

in Chemistry,

3l,

S27,

1993