1H NMR Sequential Assignments of Oxidized Ferredoxins from CLOSTRIDIUM and CLOSTRIDIUM
PASTEURIANUM
ACIDURICI
Jacques Gaillard*, Jean-Marc Moulis#, Rainer Kiimmerle* and Jacques Meyerb, CEA, Departement de Recherche sur la Matiere Condensee, SESAM-SCPM (*) and Departement de Biologie Moleculaire et Structurale, Laboratoire des Metalloproteines (#) CENG, 17 Rue des Martyrs, 38054 GRENOBLE CEDEX, France The two 2[4Fe-4S] ferredoxins from C. pasteurianum have
and C. acidurici NMR.
Using
experiments,
been
investigated
conventional sequential
TOCSY
assignments
by 'H 2D
and NOESY were obtained
for the two segments at the N- and C-termini
and
for the segment between residues 23 and 36. Taking advantage
of
sequence
homology
between
the
two
proteins,
further assignments have been made to a
total of about 80% of the sequence. The
presence
of
the
two
paramagnetic
centers strongly influences the relaxation properties of the neighbouring protons. Nevertheless, some long range NCE correlations analysis,
structural
were displayed.
informations
were
From their extracted:
the contact between the two terminal parts of the proteins
is a highly
conserved
feature
but
small
differences were evidenced between the two proteins and
are
to
be
related to variations observed between some properties of these ferredoxins. J. Gaillard, Magnetic