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1P-0080 Increased levels of insulin-like growth factor binding protein-3 in hypertensive patients with carotid atherosclerosis
Monday September 29, 2003: Poster Session Hypertension and atherosclerosis (BMI>25) were three times more likely to be hypertensive than non obese subjects. Obesity and hypertension in both urban and rural areas were more pronounced in women than in men. Conclusions: It is concluded that prevalence of hypertension and obesity are higher in city than villages and female than male. These findings point to the necessity of controlling these parameters by correcting food habits, physical exercise and education of the public. 1P-0080
Increased levels of insulin-like growth factor binding protein-3 in hypertensive patients with carotid atherosclerosis
T. Watanabe 1 , M. Itokawa 1 , T. Katagiri 2 . 1 Department of Internal Medicine; 2 Third Department of Internal Medicine, Showa University School of Medicine, Japan
1P-0081
Effect of angiotensin II type I receptor gene polymorphism on blood pressure and aortic stiffness among Malays
A. Rehman 1 , S.B. Ismail 2 , L. Naing 1 , R. Abdul Rahman 3 . 1 School of Dental Sciences; 2 School of Medical Sciences, Universiti Sains Malaysia; 3 Institute for Advanced Medical & Dental Sciences, Malaysia Objectives: To investigate an association between A1166C polymorphism of AT1R gene with HT and aortic stiffness among Malays. Methods: A hundred and seventy-one HT subjects without evidence of cardiovascular complications and 132 age- and sex-matched normotensive (NT) subjects without family history of hypertension gave informed consent for genetic analysis. A1166C polymorphism of AT1R gene was detected by polymerase chain reaction and DdeI restriction endonuclease digestion. Measurements included height, hip and waist circumference, body mass index (BMI), blood pressure (BP) and carotid-femoral pulse wave velocity (PWV; a measure of aortic stiffness) done using the Complior® (Colson, France) and laboratory measures. Data was analyzed using ANCOVA and multiple linear regression analysis. Results: The prevalence of C1166 allele was not different between HT (7.4%) and NT (7.5%) subjects. A1166C polymorphism had no association with BP and PWV. However when the possible confounders such as cholesterol, triglycerides, BMI, age and sex were included in the model, C1166 allele showed a borderline significant association with PWV (p=0.049). Subjects with C1166 allele (AC/CC) had a higher mean PWV than those with AA alleles (10.49 versus 9.8). Influence of C1166 allele on relationship between BP and PWV, checked by interaction between BP and gene, was not significant (p=0.661). In the HT group alone, C1166 allele had no significant association with PWV (p=0.12). Conclusions: Our data suggests that A1166C polymorphism of AT1R gene is not associated with BP but may have a small influence on aortic stiffness measured as PWV. Also C1166 allele has no influence on the relationship between BP and PWV in our study population.
To our knowledge this is the first study to report the frequency of C1166 allele and its association with BP and PWV among Malays. 1P-0082
Hypotensive effect of pravastatin in patients with mild hypertension and hypercholesterolemia
I. Tvaladze. National Research Center for Preventive Medicine, Moscow, Russia Aim: To study the influence of Pravastatin on arterial blood pressure (ABP) level in patients with mild hypertension and hypercholesterolemia. Methods: In the randomized double blind placebo controlled trial were included 54 men with mild hypertension and hypercholesterolemia. The duration - 12 weeks. The patients were randomized in three groups: I group (18 patients) - Placebo, II group (18 patients) - Pravastatin 40 mg/day, III group (18 patients) - Diltiazem 180 mg/day. To estimate objectively of ABP level, as the result of the treatment with Pravastatin, Diltiazem and Placebo it was carried out ambulatory measurement of ABP by the 24-hour monitoring BP. Results: Pravastatin significantly reduced mean 24-hour systolic and diastolic ABP levels [SABP level from 139 ±2.1 to 133±2.1 mmHg (% -4.0±0.2) and DABP level from 88±1.6 to 83±1.6 mmHg ( % -6.2±0.3)], (p<0.001). In the placebo group - systolic and diastolic ABP levels were not reduced: SABP level was 138±2.9 mmHg-before treatment and 138±2.6 mmHg (% -0.04±0.4)-after treatment. DABP level was 89±2.1 mmHgbefore treatment and 89±1.9 mmHg (% 0.4±0.6)-after treatment. In the Diltiazem group - systolic and diastolic ABP levels were significantly reduced [SABP level from 147±1.9 to 137±1.7 mmHg (% -6.8±0.2) and DABP level from 93.1±1.1 to 84.1±1.1 mmHg (% -9.7±0.3)], (p<0.001). In the Pravastatin group mean LDL cholesterol level was reduced from 204±7.1 to 122±9.5 mg/dl (% -37.7±4.4), (p<0.001). In the placebo group was obtained unauthentic results: mean LDL cholesterol level was 178±14.0 mg/dl - before treatment and 182±14.0 mg/dl ( % 2.1±1.6)-after treatment. In the Diltiazem group mean LDL cholesterol level was reduced from 177±6.8 to 160±3.4 mg/dl (% -9.3±2.1), (p<0.01). Conclusion: Pravastatin reduced both LDL cholesterol level and 24-hour systolic and diastolic ABP levels in patients with mild hypertension and hypercholesterolemia. 1P-0083
The relationships between pulse wave velocity and blood pressure or diabetes mellitus in male outpatients
N. Satio 1 , T. Kannagi 2 , H. Sayama 2 . 1 Miyazaki Aiwa Hospital, Miyazaki City; 2 Kyoto Preventive Medical Center, Japan Pulse wave velocity (PWV) is correlated to aging and atherosclerosis. This study aimed to clarify the relationships between PWV and blood pressure, intima-media complex, the plaques or diabetes mellitus in male outpatients. Methods: 1) In this study 44 male outpatients aged 51 to 68 years were recruited, who were divided into two groups according to the presence (n=17) or absence (n=27) of the plaques in carotid arteries. 2) When glucose tolerance was determined through 75gOGTT, 62 male diabetic and 14 male normal types were recruited, who were aged 40 to 69 years. Fasting blood sample was obtained early in the morning for measuring biochemical data. Arm and leg blood pressures, PWV and ankle-brachial index (ABI) were measured using form PWV/ABI. The widths of intima-media complex and the height of plaque in carotid artery were measured using neck echograpy. Results: 1) Outpatients with plaques showed significantly higher levels of systolic blood pressure (SBP) and pulse pressure (PP) as compared to ones without plaques (mean 146 mmHg: 134 mmHg in SBP, 60 mmHg: 51 mmHg in PP), and were associated significantly with higher PWV (1,905 cm/s: 1,663 cm/s). In outpatients aged 51 to 68 years, PWV had significantly positive correlations to systolic and diastolic blood pressure (r=0.897 and r=0.752). 2) In outpatients aged 40 to 69 years, diabetic type outpatients showed significantly higher PWV as compared to normal type ones (1,684 cm/s: 1,472 cm/s). The frequence of plaque in carotid artery was 48.3% in diabetics and 32.3% in normals. Blood pressure was higher in diabetics than in normals (134 mmHg: 123 mmHg in SBP), indicating the influence of SBP on PWV in diabetic outpatients. Conclusion: PWV was intimately related to blood pressure levels. The presence of plaque in carotid artery may predict the elevated PWV.
XIIIth International Symposium on Atherosclerosis, September 28–October 2, 2003, Kyoto, Japan
MONDAY
Background: It has been proven that the intima-media thickness (IMT) of the carotid artery increases in patients with essential hypertension. Serum levels of insulin-like growth factor-I (IGF-I) increase in hypertensive patients with ventricular hypertrophy. However, the relationship between carotid atherosclerosis and serum levels of IGF-I and its binding protein-3 (IGFBP-3) in patients with essential hypertension has not been established. Methods: The carotid IMT, blood pressure (BP), serum lipid profiles, and serum IGF-I and IGFBP-3 contents were determined in 54 hypertensive patients (19 with and 35 without carotid plaque) and 52 normotensive controls without plaque. Results: Systolic, diastolic, and mean BPs and serum IGFBP-3 level were significantly higher in the hypertensive patients (with and without plaque) than in the normotensive controls. Serum IGFBP-3 level was significantly correlated with systolic BP (r = 0.204, P = 00354). Age, gender, body mass index, and serum levels of HDL cholesterol, triglyceride, lipoprotein(a), lipid peroxides, insulin, and fasting plasma glucose levels did not differ significantly among the three groups. Hypertensive patients with plaque, compared with those without plaque or the normotensive controls, had the highest values of carotid IMT, LDL cholesterol, IGF-I, and IGFBP-3. Multiple logistic regression analysis revealed that the IGFBP-3 level was associated with a 9-fold (95% CI: 2.6-31) higher risk of carotid plaque formation compared with LDL cholesterol or IGF-I levels. Conclusions: These results suggest that an increased level of IGFBP-3 may play a crucial role in the development of carotid atherosclerosis in hypertensive patients.
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Increased levels of insulin-like growth factor binding protein-3 in hypertensive patients with carotid atherosclerosis
T. Watanabe 1 , M. Itokawa 1 , T. Katagiri 2 . 1 Department of Internal Medicine; 2 Third Department of Internal Medicine, Showa University School of Medicine, Japan
1P-0081
Effect of angiotensin II type I receptor gene polymorphism on blood pressure and aortic stiffness among Malays
A. Rehman 1 , S.B. Ismail 2 , L. Naing 1 , R. Abdul Rahman 3 . 1 School of Dental Sciences; 2 School of Medical Sciences, Universiti Sains Malaysia; 3 Institute for Advanced Medical & Dental Sciences, Malaysia Objectives: To investigate an association between A1166C polymorphism of AT1R gene with HT and aortic stiffness among Malays. Methods: A hundred and seventy-one HT subjects without evidence of cardiovascular complications and 132 age- and sex-matched normotensive (NT) subjects without family history of hypertension gave informed consent for genetic analysis. A1166C polymorphism of AT1R gene was detected by polymerase chain reaction and DdeI restriction endonuclease digestion. Measurements included height, hip and waist circumference, body mass index (BMI), blood pressure (BP) and carotid-femoral pulse wave velocity (PWV; a measure of aortic stiffness) done using the Complior® (Colson, France) and laboratory measures. Data was analyzed using ANCOVA and multiple linear regression analysis. Results: The prevalence of C1166 allele was not different between HT (7.4%) and NT (7.5%) subjects. A1166C polymorphism had no association with BP and PWV. However when the possible confounders such as cholesterol, triglycerides, BMI, age and sex were included in the model, C1166 allele showed a borderline significant association with PWV (p=0.049). Subjects with C1166 allele (AC/CC) had a higher mean PWV than those with AA alleles (10.49 versus 9.8). Influence of C1166 allele on relationship between BP and PWV, checked by interaction between BP and gene, was not significant (p=0.661). In the HT group alone, C1166 allele had no significant association with PWV (p=0.12). Conclusions: Our data suggests that A1166C polymorphism of AT1R gene is not associated with BP but may have a small influence on aortic stiffness measured as PWV. Also C1166 allele has no influence on the relationship between BP and PWV in our study population.
To our knowledge this is the first study to report the frequency of C1166 allele and its association with BP and PWV among Malays. 1P-0082
Hypotensive effect of pravastatin in patients with mild hypertension and hypercholesterolemia
I. Tvaladze. National Research Center for Preventive Medicine, Moscow, Russia Aim: To study the influence of Pravastatin on arterial blood pressure (ABP) level in patients with mild hypertension and hypercholesterolemia. Methods: In the randomized double blind placebo controlled trial were included 54 men with mild hypertension and hypercholesterolemia. The duration - 12 weeks. The patients were randomized in three groups: I group (18 patients) - Placebo, II group (18 patients) - Pravastatin 40 mg/day, III group (18 patients) - Diltiazem 180 mg/day. To estimate objectively of ABP level, as the result of the treatment with Pravastatin, Diltiazem and Placebo it was carried out ambulatory measurement of ABP by the 24-hour monitoring BP. Results: Pravastatin significantly reduced mean 24-hour systolic and diastolic ABP levels [SABP level from 139 ±2.1 to 133±2.1 mmHg (% -4.0±0.2) and DABP level from 88±1.6 to 83±1.6 mmHg ( % -6.2±0.3)], (p<0.001). In the placebo group - systolic and diastolic ABP levels were not reduced: SABP level was 138±2.9 mmHg-before treatment and 138±2.6 mmHg (% -0.04±0.4)-after treatment. DABP level was 89±2.1 mmHgbefore treatment and 89±1.9 mmHg (% 0.4±0.6)-after treatment. In the Diltiazem group - systolic and diastolic ABP levels were significantly reduced [SABP level from 147±1.9 to 137±1.7 mmHg (% -6.8±0.2) and DABP level from 93.1±1.1 to 84.1±1.1 mmHg (% -9.7±0.3)], (p<0.001). In the Pravastatin group mean LDL cholesterol level was reduced from 204±7.1 to 122±9.5 mg/dl (% -37.7±4.4), (p<0.001). In the placebo group was obtained unauthentic results: mean LDL cholesterol level was 178±14.0 mg/dl - before treatment and 182±14.0 mg/dl ( % 2.1±1.6)-after treatment. In the Diltiazem group mean LDL cholesterol level was reduced from 177±6.8 to 160±3.4 mg/dl (% -9.3±2.1), (p<0.01). Conclusion: Pravastatin reduced both LDL cholesterol level and 24-hour systolic and diastolic ABP levels in patients with mild hypertension and hypercholesterolemia. 1P-0083
The relationships between pulse wave velocity and blood pressure or diabetes mellitus in male outpatients
N. Satio 1 , T. Kannagi 2 , H. Sayama 2 . 1 Miyazaki Aiwa Hospital, Miyazaki City; 2 Kyoto Preventive Medical Center, Japan Pulse wave velocity (PWV) is correlated to aging and atherosclerosis. This study aimed to clarify the relationships between PWV and blood pressure, intima-media complex, the plaques or diabetes mellitus in male outpatients. Methods: 1) In this study 44 male outpatients aged 51 to 68 years were recruited, who were divided into two groups according to the presence (n=17) or absence (n=27) of the plaques in carotid arteries. 2) When glucose tolerance was determined through 75gOGTT, 62 male diabetic and 14 male normal types were recruited, who were aged 40 to 69 years. Fasting blood sample was obtained early in the morning for measuring biochemical data. Arm and leg blood pressures, PWV and ankle-brachial index (ABI) were measured using form PWV/ABI. The widths of intima-media complex and the height of plaque in carotid artery were measured using neck echograpy. Results: 1) Outpatients with plaques showed significantly higher levels of systolic blood pressure (SBP) and pulse pressure (PP) as compared to ones without plaques (mean 146 mmHg: 134 mmHg in SBP, 60 mmHg: 51 mmHg in PP), and were associated significantly with higher PWV (1,905 cm/s: 1,663 cm/s). In outpatients aged 51 to 68 years, PWV had significantly positive correlations to systolic and diastolic blood pressure (r=0.897 and r=0.752). 2) In outpatients aged 40 to 69 years, diabetic type outpatients showed significantly higher PWV as compared to normal type ones (1,684 cm/s: 1,472 cm/s). The frequence of plaque in carotid artery was 48.3% in diabetics and 32.3% in normals. Blood pressure was higher in diabetics than in normals (134 mmHg: 123 mmHg in SBP), indicating the influence of SBP on PWV in diabetic outpatients. Conclusion: PWV was intimately related to blood pressure levels. The presence of plaque in carotid artery may predict the elevated PWV.
XIIIth International Symposium on Atherosclerosis, September 28–October 2, 2003, Kyoto, Japan
MONDAY
Background: It has been proven that the intima-media thickness (IMT) of the carotid artery increases in patients with essential hypertension. Serum levels of insulin-like growth factor-I (IGF-I) increase in hypertensive patients with ventricular hypertrophy. However, the relationship between carotid atherosclerosis and serum levels of IGF-I and its binding protein-3 (IGFBP-3) in patients with essential hypertension has not been established. Methods: The carotid IMT, blood pressure (BP), serum lipid profiles, and serum IGF-I and IGFBP-3 contents were determined in 54 hypertensive patients (19 with and 35 without carotid plaque) and 52 normotensive controls without plaque. Results: Systolic, diastolic, and mean BPs and serum IGFBP-3 level were significantly higher in the hypertensive patients (with and without plaque) than in the normotensive controls. Serum IGFBP-3 level was significantly correlated with systolic BP (r = 0.204, P = 00354). Age, gender, body mass index, and serum levels of HDL cholesterol, triglyceride, lipoprotein(a), lipid peroxides, insulin, and fasting plasma glucose levels did not differ significantly among the three groups. Hypertensive patients with plaque, compared with those without plaque or the normotensive controls, had the highest values of carotid IMT, LDL cholesterol, IGF-I, and IGFBP-3. Multiple logistic regression analysis revealed that the IGFBP-3 level was associated with a 9-fold (95% CI: 2.6-31) higher risk of carotid plaque formation compared with LDL cholesterol or IGF-I levels. Conclusions: These results suggest that an increased level of IGFBP-3 may play a crucial role in the development of carotid atherosclerosis in hypertensive patients.
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