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2016 Role of the parabrachial nucleus in salivation induced by rejectable taste in rats
S223 2014 LONG-TERM AND SHORT-TERM PLASTICITY IN THE DENDRO-DENDRITIC MITRAL-TOGRANULE CELL SYNAPSE OF THE TELEOST OLFACTORY BULB. SHIHO ANZAI, WASAHIKO SATOU. Grad. Sch. of Inteur. Sci., Yokohama City Univ., Yokohama 236, Japan.
We tested whether or not there is synaptic plasticity in the teleost (carp, Cyprinus carpio L.) olfactory bulb. Low-frequency tetanic stimuli (5 or 10 Hz, 201 pulses) were applied to the olfactory tract, and the changes in the synaptic efficacy of the dendro-dendritic mitral-to-granule cell synapse were examined by measuring the size of the field potential response (C2 copmponent). As the response greatly increased results, in all cases tested the field potential shortly after the tetanus. Thereafter, the increased phase of the synaptic l-3 hours in several cases (long-term transmission lasted for more than plasticity), while it recovered to the level before the tetanus within 1 hour in other cases (short-term plasticity). Long-term plasticity occurred when the granule cells were activated simultaneously both through the mitral-to-granule cell synapse and through the synapse from the centrifugal fibers. Moreover, EEGlike field potential response (induced wave) to odor stimulation also incresed in size after the tetanus.
2015
Optical study of central olfactory processing in the terrestrial slug.
ShoichiTodal, Tetsuya Kimura2, ShigenotiKawaharal,TatsuhikoSekiguchiz,and Yutaka Kirino* 1Faculty of PharmaceuticalSciences,The University of Tokyo, 7-3- 1 Hongo, Bunkyo-ku, Tokyo 113,Japan 2TsukubaResearchCenter, SANYO Electric Co. Ltd., 2-l Koyadai, Tsukuba305, Japan In the terrestrial slugLima, olfactory information seemsto be processedin the procerebral(PC) lobe of the cerebral ganglionsincethe PC lobereceivesinputsfrom olfactory nerves,andexhibits coherentoscillationof membranepotentials which is modulatedby odor. To investigatethe mechanismof this olfactory modulation, we measuredthe spatio-temporal patternof the oscillatory activity of the PC during the odor stimulusby usingthe optical imagingtechnique.Duringthe odor stimulus,the neuropil of the PC exhibited a transientriseof the level of hyperpolarization peakof the oscillation while the cell body layer did not. This hyperpolarization peak level of the neuropiland the frequency of the PC oscillationare highly correlated.Thesefindingssuggestthe neuropil areaplays an importantrole of the regulationof PC oscillationfrequency
ROLE OF THE PARABRACHIAL NUCLEUS IN SALIVATION INDUCED BY REJECTABLE TASTE IN RATS. YAMAUCHI,Y. l, MAISU0,R.2, NOKUBLT. IL MORIMOT0,T.2The 2nd Dept. of Prosthetic Dentistryi, and Dept. of Oral Physiology2, Faculty of Dentistry, Osaka Univ., Osaka, 565 , Japan. 2016
In response to orally injected bitter substance, rats execute taste rejection behavior accompanied with vigorous salivation. This salivary secretion was abolished by injection of local anesthesia into the parabrachial nucleus (PBN). Histochemically, PBN neurons were labelled by injection of Fluoro-Gold or HRP into the superior salivatory nucleus, suggesting existence of a direct connection between these two nuclei. Electrophysiologically, about 20% taste-responsive PBN neurons were antidromically activated by electrical stimulation of the superior salivatory nucleus.
We tested whether or not there is synaptic plasticity in the teleost (carp, Cyprinus carpio L.) olfactory bulb. Low-frequency tetanic stimuli (5 or 10 Hz, 201 pulses) were applied to the olfactory tract, and the changes in the synaptic efficacy of the dendro-dendritic mitral-to-granule cell synapse were examined by measuring the size of the field potential response (C2 copmponent). As the response greatly increased results, in all cases tested the field potential shortly after the tetanus. Thereafter, the increased phase of the synaptic l-3 hours in several cases (long-term transmission lasted for more than plasticity), while it recovered to the level before the tetanus within 1 hour in other cases (short-term plasticity). Long-term plasticity occurred when the granule cells were activated simultaneously both through the mitral-to-granule cell synapse and through the synapse from the centrifugal fibers. Moreover, EEGlike field potential response (induced wave) to odor stimulation also incresed in size after the tetanus.
2015
Optical study of central olfactory processing in the terrestrial slug.
ShoichiTodal, Tetsuya Kimura2, ShigenotiKawaharal,TatsuhikoSekiguchiz,and Yutaka Kirino* 1Faculty of PharmaceuticalSciences,The University of Tokyo, 7-3- 1 Hongo, Bunkyo-ku, Tokyo 113,Japan 2TsukubaResearchCenter, SANYO Electric Co. Ltd., 2-l Koyadai, Tsukuba305, Japan In the terrestrial slugLima, olfactory information seemsto be processedin the procerebral(PC) lobe of the cerebral ganglionsincethe PC lobereceivesinputsfrom olfactory nerves,andexhibits coherentoscillationof membranepotentials which is modulatedby odor. To investigatethe mechanismof this olfactory modulation, we measuredthe spatio-temporal patternof the oscillatory activity of the PC during the odor stimulusby usingthe optical imagingtechnique.Duringthe odor stimulus,the neuropil of the PC exhibited a transientriseof the level of hyperpolarization peakof the oscillation while the cell body layer did not. This hyperpolarization peak level of the neuropiland the frequency of the PC oscillationare highly correlated.Thesefindingssuggestthe neuropil areaplays an importantrole of the regulationof PC oscillationfrequency
ROLE OF THE PARABRACHIAL NUCLEUS IN SALIVATION INDUCED BY REJECTABLE TASTE IN RATS. YAMAUCHI,Y. l, MAISU0,R.2, NOKUBLT. IL MORIMOT0,T.2The 2nd Dept. of Prosthetic Dentistryi, and Dept. of Oral Physiology2, Faculty of Dentistry, Osaka Univ., Osaka, 565 , Japan. 2016
In response to orally injected bitter substance, rats execute taste rejection behavior accompanied with vigorous salivation. This salivary secretion was abolished by injection of local anesthesia into the parabrachial nucleus (PBN). Histochemically, PBN neurons were labelled by injection of Fluoro-Gold or HRP into the superior salivatory nucleus, suggesting existence of a direct connection between these two nuclei. Electrophysiologically, about 20% taste-responsive PBN neurons were antidromically activated by electrical stimulation of the superior salivatory nucleus.