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203 HORMONE STATUS OF PREGNANT WOMEN AND NEWBORN BODY WEIGHT
Reproductive BioMedicine Online 20 Suppl. 3 (2010) S94–S98
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Reproductive Endocrinology 202 EVALUATION OF REPRODUCTION FUNCTION IN HUMAN USED ULTRASENSITIVE METHOD TO MEASURE BIOLOGICAL ACTIVE FREE TESTOSTERONE N.P. Goncharov1 , G.V. Katsya1 , N.M. Malysheva1 , O.A. Melihova2 , V.P. Smetnik2 . 1 Endocrinology Research Center, Moscow, Russia, 2 Research Center of Obstetrics, Gynecology and Perinatology, Moscow, Russia
informative biomarker for assessment of androgen status in men and women in different period of life.
Objectives: According to our own and others experiences direct methods measure level of total testosterone (TT) unacceptable for women and children, and critical for men (TT<10 nmol/L). The aim of the study was evaluation of ELISA and enhances luminescence (LIA) methods for measuring free testosterone (FT) in saliva and sera, and calculated FT (CFT) as well, and use it for assessment of androgen status.
About 21 million children with low weight are born every year, according to the data from World Health Organization. In this connection development of effective and reliable methods for diagnosing intrauterine growth restriction (IUGR) is one of the most important areas of perinatal medicine. Thereby the aim of our research was to investigate levels of serum hormones in women with normal pregnancy and in those who delivered the infants with low birth weight. We assayed fasting serum samples from 86 women at 15 18 weeks of gestation. Control group consisted of women (n = 57) with normal pregnancies, who delivered infants with no birth defects, chromosomal abnormalities and IUGR. Case group included women (n = 29) who delivered the low birth weight infants (2000
Methods: Serum and saliva samples were taken in morning from 189 men in age 22 75 years and 58 women 18 69 years. TT and SHBG in serum were measured by automated analyzers Vitros ECi and Elecsys2010 respectively. FT in sera after ultrafiltration (UF) (Millipore’s Amicon Ultra-4 centrifugal filter devices 30K) and in saliva was measured quantitatively to compare results by LIA and ELISA. Results: See the table. LIA. Table. Levels of FT (median and 10th -90th percentiles). Men
TT nmol/l
FT pmol/L Saliva
TT>10 nmol/l (n = 148) TT<10 nmol/l (n = 41)
16.9 13.1 21.4 8.2 5.7 9.3
In blood (CFT) Serum (UF)
393 298 312 534 253 404 262 159 219 357 113 197
148 101 199 83 33 126
Percent of revealing of T deficiency in men determine by different methods: FT (UF) 38%, TT 20%, FT in saliva 18%, CFT 15%. Salivary T concentration in healthy women 17 39 years was 87(55 210), in postmenopausal group 73(25 116) and androgen deficient women 23(18 250 pmol/L). Androgen deficiency is more profound after ovariectomy in comparison with menopausal group. Measurement of FT in saliva and ultrafiltrate with ELISA unacceptable, it produced significant positive bias in comparison with LIA. Conclusions: The level of FT measured by LIA method in serum after UF and in saliva can be recommended to use as additional sensitive and
203 HORMONE STATUS OF PREGNANT WOMEN AND NEWBORN BODY WEIGHT L.V. Gutnikova, A.A. Aleksandrova, T.P. Shkurat. Research Institute for Biology, SFedU, Rostov-on-Don, Russia
1472-6483/$ - see front matter © 2010, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.
www.sciencedirect.com www.rbmonline.com
Reproductive Endocrinology 202 EVALUATION OF REPRODUCTION FUNCTION IN HUMAN USED ULTRASENSITIVE METHOD TO MEASURE BIOLOGICAL ACTIVE FREE TESTOSTERONE N.P. Goncharov1 , G.V. Katsya1 , N.M. Malysheva1 , O.A. Melihova2 , V.P. Smetnik2 . 1 Endocrinology Research Center, Moscow, Russia, 2 Research Center of Obstetrics, Gynecology and Perinatology, Moscow, Russia
informative biomarker for assessment of androgen status in men and women in different period of life.
Objectives: According to our own and others experiences direct methods measure level of total testosterone (TT) unacceptable for women and children, and critical for men (TT<10 nmol/L). The aim of the study was evaluation of ELISA and enhances luminescence (LIA) methods for measuring free testosterone (FT) in saliva and sera, and calculated FT (CFT) as well, and use it for assessment of androgen status.
About 21 million children with low weight are born every year, according to the data from World Health Organization. In this connection development of effective and reliable methods for diagnosing intrauterine growth restriction (IUGR) is one of the most important areas of perinatal medicine. Thereby the aim of our research was to investigate levels of serum hormones in women with normal pregnancy and in those who delivered the infants with low birth weight. We assayed fasting serum samples from 86 women at 15 18 weeks of gestation. Control group consisted of women (n = 57) with normal pregnancies, who delivered infants with no birth defects, chromosomal abnormalities and IUGR. Case group included women (n = 29) who delivered the low birth weight infants (2000
Methods: Serum and saliva samples were taken in morning from 189 men in age 22 75 years and 58 women 18 69 years. TT and SHBG in serum were measured by automated analyzers Vitros ECi and Elecsys2010 respectively. FT in sera after ultrafiltration (UF) (Millipore’s Amicon Ultra-4 centrifugal filter devices 30K) and in saliva was measured quantitatively to compare results by LIA and ELISA. Results: See the table. LIA. Table. Levels of FT (median and 10th -90th percentiles). Men
TT nmol/l
FT pmol/L Saliva
TT>10 nmol/l (n = 148) TT<10 nmol/l (n = 41)
16.9 13.1 21.4 8.2 5.7 9.3
In blood (CFT) Serum (UF)
393 298 312 534 253 404 262 159 219 357 113 197
148 101 199 83 33 126
Percent of revealing of T deficiency in men determine by different methods: FT (UF) 38%, TT 20%, FT in saliva 18%, CFT 15%. Salivary T concentration in healthy women 17 39 years was 87(55 210), in postmenopausal group 73(25 116) and androgen deficient women 23(18 250 pmol/L). Androgen deficiency is more profound after ovariectomy in comparison with menopausal group. Measurement of FT in saliva and ultrafiltrate with ELISA unacceptable, it produced significant positive bias in comparison with LIA. Conclusions: The level of FT measured by LIA method in serum after UF and in saliva can be recommended to use as additional sensitive and
203 HORMONE STATUS OF PREGNANT WOMEN AND NEWBORN BODY WEIGHT L.V. Gutnikova, A.A. Aleksandrova, T.P. Shkurat. Research Institute for Biology, SFedU, Rostov-on-Don, Russia
1472-6483/$ - see front matter © 2010, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.