- Email: [email protected]
[203-POS]
102
Abstracts / Pregnancy Hypertension: An International Journal of Women’s Cardiovascular Health 5 (2015) 53–156
Sociology, University of Lagos, Lagos, Nigeria, b Department of Obstetrics and Gynaecology, and the Child and Family Research Unit, University of British Columbia, Vancouver, BC, Canada, c Department of Fetal and Maternal Health, Olabisi Onabanjo University, Sagamu, Nigeria, d Division of Women and Child Health, Aga Khan University, Karachi, Pakistan, e Centre for Research in Reproductive Health, Sagamu, Nigeria) Objectives: The objective of this study was to describe community perceptions of pre-eclampsia and eclampsia in Ogun State. Background: Pre-eclampsia is a common complication of pregnancy and is responsible for high rates of morbidity and mortality, especially in less developed countries. While most studies related to pre-eclampsia and eclampsia have adopted a bio-medical model, this paper adopts a perspective which recognizes the role of the socio-cultural environment. Methods: The study was conducted in four Local Government Areas in Ogun State, Nigeria in 2012. Data were obtained through 24 focus group discussions with pregnant women (N = 94), mothers with children less than 5 years old (N = 95), male decision makers (N = 47), community leaders (N = 56), and traditional birth attendants (N = 36). In addition, nine in-depth interviews were conducted with the head of the local traditional birth attendants (N = 4), a local traditional birth attendant (N = 1), and community leaders (N = 4). Results: We determined that there are no names for preeclampsia in the local language, Yoruba, although ’’hypertension’’ and ’’convulsion’’ as disease entities have local names that are independent of pregnancy status. The cause of pre-eclampsia was perceived to be due to depressive thoughts, and the cause of eclampsia was perceived to result from exposure to cold with a few implicating spiritual forces. While, there seemed to be no local treatment for pre-eclampsia apart from preventive practices, local treatments for eclampsia included the use of herbs, concoctions, incisions, and black soap. Conclusions: This study shows that knowledge of preeclampsia and its progression to eclampsia is limited. It also reveals a gap in knowledge of the etiology and treatment of the condition. A holistic approach is recommended for sensitization at the community level, acquisition of educational skills by health workers, and the adoption of a community perspective as a sustainable approach to reduce the complications of pregnancy. Disclosures: D.O. Akeju: None. M. Vidler: None. O.T. Oladapo: None. D. Sawchuck: None. R. Qureshi: None. P. von Dadelszen: None. O.O. Adetoro: None. K. Dada: None.
Sarah Hart a, Louise Kenny b, Jenny Myers c, Philip Baker a (a Keele University, Stoke on Trent, United Kingdom, b INFANT Centre, Cork, Ireland, c University of Manchester, Manchester, United Kingdom) Objectives: Urine as a biofluid is commonly used in clinical diagnostics, including during pregnancy. Urine is a rich source of polypeptides and protein degradation products. Electron transfer dissociation (ETD) techniques have recently been applied to peptides, and improved fragmentation of long polypeptides has been demonstrated; ETD further facilitates analysis of labile post-translational modifications. We therefore applied this advanced technique for peptide dissociation to analyse and identify urinary polypeptides for the first time. Methods: We examined the urinary peptidome from normal pregnant women during pregnancy, to demonstrate that peptides are readily observed. We utilised conventional MS/ MS techniques and then used ETD (LC–MS/MS performed on an orbitrap equipped with ETD source, coupled to a nanoAcquity HPLC) to increase the identification rate of the peptides within these samples, as the polypeptide species observed are large and highly charged. Results: An increase in the number of peptides whose identities could be ascribed using routine database searching methods was enabled via use of ETD. These peptides, as anticipated, were large and highly charged (typically giving charge states of 4–8+ over a mass-to-charge range of 400–2000 m/z). Conclusions: Urinary proteomic profiling has not been able to discriminate between normal and pre-eclampsia pregnancies at gestations early enough to enable preventative strategies. Enhanced polypeptide identification achieved through ETD may facilitate clinically useful predictive tests.
Disclosures: S. Hart: None. L. Kenny: Commercial Interest: Metabolomic Diagnostics. J. Myers: None. P. Baker: Commercial Interest: Metabolomic Diagnostics. doi:10.1016/j.preghy.2014.10.209
doi:10.1016/j.preghy.2014.10.208
[204-POS] [203-POS] Electron transfer dissociation for native peptide fragmentation facilitates enhanced identification of urinary peptides and proteins in pregnancy
Is early onset pre-eclampsia worse for mother or baby? Franziska M. Pettit, George J. Mangos, Gregory Davis, Amanda Henry, Mark A. Brown (St. George Hospital, Kogarah, Australia)
Abstracts / Pregnancy Hypertension: An International Journal of Women’s Cardiovascular Health 5 (2015) 53–156
Sociology, University of Lagos, Lagos, Nigeria, b Department of Obstetrics and Gynaecology, and the Child and Family Research Unit, University of British Columbia, Vancouver, BC, Canada, c Department of Fetal and Maternal Health, Olabisi Onabanjo University, Sagamu, Nigeria, d Division of Women and Child Health, Aga Khan University, Karachi, Pakistan, e Centre for Research in Reproductive Health, Sagamu, Nigeria) Objectives: The objective of this study was to describe community perceptions of pre-eclampsia and eclampsia in Ogun State. Background: Pre-eclampsia is a common complication of pregnancy and is responsible for high rates of morbidity and mortality, especially in less developed countries. While most studies related to pre-eclampsia and eclampsia have adopted a bio-medical model, this paper adopts a perspective which recognizes the role of the socio-cultural environment. Methods: The study was conducted in four Local Government Areas in Ogun State, Nigeria in 2012. Data were obtained through 24 focus group discussions with pregnant women (N = 94), mothers with children less than 5 years old (N = 95), male decision makers (N = 47), community leaders (N = 56), and traditional birth attendants (N = 36). In addition, nine in-depth interviews were conducted with the head of the local traditional birth attendants (N = 4), a local traditional birth attendant (N = 1), and community leaders (N = 4). Results: We determined that there are no names for preeclampsia in the local language, Yoruba, although ’’hypertension’’ and ’’convulsion’’ as disease entities have local names that are independent of pregnancy status. The cause of pre-eclampsia was perceived to be due to depressive thoughts, and the cause of eclampsia was perceived to result from exposure to cold with a few implicating spiritual forces. While, there seemed to be no local treatment for pre-eclampsia apart from preventive practices, local treatments for eclampsia included the use of herbs, concoctions, incisions, and black soap. Conclusions: This study shows that knowledge of preeclampsia and its progression to eclampsia is limited. It also reveals a gap in knowledge of the etiology and treatment of the condition. A holistic approach is recommended for sensitization at the community level, acquisition of educational skills by health workers, and the adoption of a community perspective as a sustainable approach to reduce the complications of pregnancy. Disclosures: D.O. Akeju: None. M. Vidler: None. O.T. Oladapo: None. D. Sawchuck: None. R. Qureshi: None. P. von Dadelszen: None. O.O. Adetoro: None. K. Dada: None.
Sarah Hart a, Louise Kenny b, Jenny Myers c, Philip Baker a (a Keele University, Stoke on Trent, United Kingdom, b INFANT Centre, Cork, Ireland, c University of Manchester, Manchester, United Kingdom) Objectives: Urine as a biofluid is commonly used in clinical diagnostics, including during pregnancy. Urine is a rich source of polypeptides and protein degradation products. Electron transfer dissociation (ETD) techniques have recently been applied to peptides, and improved fragmentation of long polypeptides has been demonstrated; ETD further facilitates analysis of labile post-translational modifications. We therefore applied this advanced technique for peptide dissociation to analyse and identify urinary polypeptides for the first time. Methods: We examined the urinary peptidome from normal pregnant women during pregnancy, to demonstrate that peptides are readily observed. We utilised conventional MS/ MS techniques and then used ETD (LC–MS/MS performed on an orbitrap equipped with ETD source, coupled to a nanoAcquity HPLC) to increase the identification rate of the peptides within these samples, as the polypeptide species observed are large and highly charged. Results: An increase in the number of peptides whose identities could be ascribed using routine database searching methods was enabled via use of ETD. These peptides, as anticipated, were large and highly charged (typically giving charge states of 4–8+ over a mass-to-charge range of 400–2000 m/z). Conclusions: Urinary proteomic profiling has not been able to discriminate between normal and pre-eclampsia pregnancies at gestations early enough to enable preventative strategies. Enhanced polypeptide identification achieved through ETD may facilitate clinically useful predictive tests.
Disclosures: S. Hart: None. L. Kenny: Commercial Interest: Metabolomic Diagnostics. J. Myers: None. P. Baker: Commercial Interest: Metabolomic Diagnostics. doi:10.1016/j.preghy.2014.10.209
doi:10.1016/j.preghy.2014.10.208
[204-POS] [203-POS] Electron transfer dissociation for native peptide fragmentation facilitates enhanced identification of urinary peptides and proteins in pregnancy
Is early onset pre-eclampsia worse for mother or baby? Franziska M. Pettit, George J. Mangos, Gregory Davis, Amanda Henry, Mark A. Brown (St. George Hospital, Kogarah, Australia)