22 Pituitary and luteal responsiveness to LH-RH

25s

21 GLUCORTICOIDRECEPTORSIN HUMANFETAL BRAIN Mancuso,S.,Lanzone,A.,Scambia,G,,Palomba,M.,Caruso,A.,Benedetti Dept.

of

Gynecology

Glucocorticoid

and “Histology,

hormones

development.

Recently

of

the

of

of

human fetal

brain

by dextran Binding sent tional

the

charcoal

with

were

Receptor age values

(3 to

and

from

1 to

ranged

diethylstilbestrol weakly.Our results tional

ages.

Ion, cerebellum

technique

determined

by the

diencephalon

ranging

found

and

(GR) have

from

be devoid

19 to of

of

the 3

from

present

10 to

in

54 fmoles/mg

receptors.

5 nM. Competition

Cagtiari, Nervous been

System

presence

Glucorticoid

the

of

of

6 fetuses

diencephalon,

protein.

Cerebral

(CNS)

functions

GR in

different

sites

areas

were measured

as radiolabelled GR were found to at

and

in differentareas

binding

30 fmoles/mg

The estimated stu ies performed

and OGremo,F.

ITALY

identified

H dexamethasone scatchard plots.

and cerebellum ranged

GR were

using use

nor R-1881 competed for show that GR are present

During

in Central

receptors

concentration

was always

role

Cagliari,

We investigated gestational ages

(DCC)

fetuses)

Panici,P.

of

animals.

at different

hemispheres,

gesfarional glia

experimental

coated

age.

an important

glucocorticoid

brain

parameters

in

play

University

12-13

protein.

weeks At 20-22

cerebellum cortex

ligand. be pre-

of

gesta-

weeks

and basal in

these

of

gan-

fetuses

Kd was the same during development in two cases showed that neither

4

H dexamethasone binding while R-5020 competed in the CNS of human fetuses at very early Rests-

development

GR appears to be retained in specific areas, i-c. diencephabasal gangliawhile in the cortex they become undetectable.

22

PITUITARY &ND LUTEAL ~~S~~SS

To LH-RH.

LanzoneA., Caruso A.* rUlqh@suAMt PilloniMD* RasSU G., bfanCUS0 S. - lQt.Obet@%i~ Gynecology,universityof Caqliary,Via Ospedale,46- 09100 CAGLIARY (fl%.LY) The pituitary

and

an& luteelresponsesto LE-BB and their relatedchangeswere studiedin 11 normalwomen during lutealphase&P-day +4 to +ll).Bloodsampleswere collectedevery 15 x&n for a basal periodof 180 min and 120 mln after i.v. administration of 25)q of LBBH.Proqesterone(P) and LB were assayedby BIA,LB-RBeliciteda secretoryresponseof b&h hormones in all cases.IBA(i-&eqrated secretoryarea)ofLB was greaterafter Ui-RH administrationzespettto basal value@ (.oo~) andAmax(percent maxtiw increase)accounted to 4?5*36(S.E.)% of basal concentration.Luteal responsiveness variedfrom about 115-130%to xore xaxked fncxexents.XSA of P differed from basal to stimulatedconditions(pc.05) and&sax was 166*14%.The analysisof texuporal relationship betweenP and LB secretion showedthat LB promptlyrose after LB-l&while the en~nce~~ of P levelsoccurredwfthin 31,+19 mfn after LE rise. Then P levelsxeaoheda plateau,values of wldch were constantlyabove thoseobservedin basal conditions.These changesappearto be time-related in a way similarto that found in studieson spontaneous pulsatilfty. The date from this paper show that P secretionby corpus luteumincreasesafterLE-BIi,such responsesoccurring by the discbargeof qonadotxopins and are consistentwith the presenceof close relationshipsbetweenhypothalamic,pituitaxy and lutealfunctions.