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222 Genetic analyses of skin tumours developed in a patient with hereditary nonpolyposis colorectal cancer (HNPCC)
JSID
217 COMPARATIVE STUDY OF BACTEKiAL FLORA ON THE FACiAL SKIN OF ATOPIC DERMATITIS AND HEALTHY SUB.I ‘r ‘Depattmentof JECTS. m Dermatoloav. Tovama Medical and Pharmaceutical Universitv. Tovama. Jaoan. ’ Department’ of ~Laborstory Sciences, School of Health Sc~et&, Fa& of Medicine, Kanaaawa University, Kanazawa, Japan. To study the differences of characteristics of Staphylococci on the skin of stopic dermatitis and healthy subjects, the bacterial flors of these hvo groups were sampled by a contact-plate technique. The Staphylococci were selectively isolated on mannitol salt agar medium, and identified according to 20 bicchemical reactions of the API staph system. The results indicated that face of atopic dermatitis was dominantly colonized by S.oureus. The number of Staphylococci on atopic dermatitis wss higher than that on the healthy subjects. The value of M&of ten kinds of antibiotics to Staphylococci on atopic dermatitis we.m generally higher than those on healthy subjects. The susceptibility among these ten antibiotics tested, gentamytin wss the least susceptible. It was considered that increase in number of multiple resistant-Staphylococci might be seen because of much use of them. Though the correlation between the degree of rash and skin flora wss observed, it was also necessary to study the correlation between skin surface lipids and skin flora.
218 RESULTS OF PATCH TESTING With SYNTHETIC PRIMIN. M. Suaiura‘& Havakawa.Dapadment of Dermatology, Daiko Medical Canter, Nagoya Univenlty Hospital. Nagoya, Japan. Ptimin was synthesized horn 3,4dihydro-2H-pyran and guaiacol according to L.W. Siebeh method and has been dktributed among members of the Japanese Society for Contact Dermatitll. We studied whether positive reaction to synthetic prknin is relevant to pdmuia dermatitis. Using Finn Chamber and Scanportape. we carried out 48h closed patch testing with synthetic primin 0.01 %pet on 287 cases over 13 month period from February, 1906 to March 1997. Readings were mede at 24h aaer removal according to iCDRG recommendations. Two cases with @mule dermatitis end one case having past hloty of primula denatitll showed + + reactions, and 1 case with neither pdmuia denatll nor past history of primula dermatitis showed + reaction. We considered that + reaction might bs irritant reaction. Among subjects with -and +? reactions there were no cases with primula dermatitll nor with past history of primula dermatitis. We conciuciad that synthetic pnmin b asuitable allergen for patch testing and that optimum concentration is O.Ol%pet.
219 STUDY FOR SKIN TFlSTlNCi M Dm CAUSATIVE AGENTS OF OCCUPATIONAL. CONTACT DEMMATITIS. Z. Xie*l. R. &&a&-M hgis& I lkprtmmt of Hygiene. Nagoya University School of Medicine, Nagoya, Japan. 2 Ikwlmmt of Dertnatolosy,. -. Datka Medical Center, Nagoya University X&pitai. Nat&a. Jspsa Om a 2 .war .mid from Jsnasrv 1995 to Daeatber 19%. 66 oattents 121 males and 45 t&males) wclc dlagnoscd as having cccupationai dermatitis based on their wxpstionsi capones histories, onset sltustion. clinical featuresand the results of skin testing such ss dd pteh testing qm tesdng and scratch testlaa. The pstlents ccmprised 21 bmutkkns and a badxr, 13 Bctoty uorkers. 6 dental techuiclaas and s dentists, 7 agriculture-related workers, 4 cooks, 4 ““rms. and 9 others. The most beqtmtly involved stes was hands and formrats st 69.7% (46/66). Fatty six ca~epwe-c diagnosed as allergic contact demmtilis (ACD) , I I cases as irtitsnt contact dermstitb (ICD), 6 csms ss centsct vrticsria (CU). and 3 csses as CU -paaiai by ACD. ‘Ib+re was no significant dlffmce in atopic predlsumitlon rario bshveen ACD and ICD. The causstivc -&Us were dcimnkd in 62 (93.9%) cut of 66 mses: 20 (W.%) of lhe 22 barbx and beauliciam 13 WM%~ of the f&&w wrkers. 5 (71.4%) of the 7 dentist and ddd tefhttician~. ali (im~~~gticdltwweiakd~0rke~ail 4'(1oos)~00k~. all 4 (100%) nurses, and all 9 (lOti%) others. We mncludui that skin testing is sadid for
determining the causative sgcnt.8ofwork-related coatact dermstltis.
139
Abstracts
220 INDUCTION OF SUBCUTANEOUS TISSUE FIBROSIS IN NEWBORN MICE BY SIMULTANEOUS APPLICA7”3N OF TRANSFORMING GROWTH FACTOR p AND BASIC FIBRCfHLAST GROWTH FACTOR M. Shinozaki , S. Kawara, K. Takehara Department of Dermatology, Faculty of Medicine, Kanazawa University. To establish an appropriate animal model of skin fibrosis by exogenous application of growth factors, we have investigated the in viva effects of subcutaneous coinjection of TGF-S and other growth factors such as b-FGF, PDGF, and BGF in new born mice. Striking induction of granulation tissue and fibrosis was observed at the injection site after 3 consecutive days or 7 consecutive days coinjection of TGF-R and b-FGF compared with that of injected with TGF-p alone. Further, this change Persisted for
at least 2 weeks. Thus, we succeeded in establishing an animal model of skin fibrotic
disorders.
221 DETECTION OF SPECtFlC ANTIBODIES TO VASCULAR ENDOTHELIAL CELLS IN THE SERA OF PATIENTS WITH BEHCET’S DISEASE.& S. Cha’. D. Bang’. E.-S. Lee’, S. Lee’. K. H. Lee’. ‘Department of Dermatology, Yonsei Unhrersity College of Medldne, Seoul, Korea. ‘Department oi Dermatology, A]ou University Schoolof Medicine. Suwon, Korea. Autoantibodies that bind to endothelial ceils have been identlli in patlents with several forms of vascuiHls. We have detected clrculatlng antibodies to cultured human dermai mlcmvascular endothelial ceiis(HDMEC) in the sera of Behcet’s disease(BD) and other vascutar disorders including systemic lupus eryihematosus(SLE) by ELISA, SDS-PAGE and Western Molting and have observed the difference in activity of antibxiv fmm BD cOmDared with that fmm other vascular disorders. IgM antibody reacted with HDMEC surface antigen in the patients wah ED. In SLE, both IgG and IgM antibodies reacted with endothellal cell surface antlgens. in Western biotting. IgG-posiiive BD serum reacted with high molecular weighi antigen greater than 100kDa. IgG-poslthre SLE serum reacted with two omteln bands of 3CtkDa and S&Da. BD setum wntalnlng anti-endolheiial cell igM antibody reacted with 44kDa and SLE serum with 61 kDa. In dot blotting and ELISA using 44kDa HDMEC surface antigen, oniy IgM-positive BD sera reacted with the amlgen. The resuiis indicate that there are anti-endothelial cell IgM antibodies in the sera of BD, reacting with 44kDa endothelial cell surface antigen and suggest that vaacullils in BD may be caused by vascular damage due to specillc antibodies against endotheiial cells.
222 GENETIC ANALYSES OF SKIN TUMOURS DEVELOPED IN A PATIENT WTTH HEREDITARY NONPOLYPOSIS COLORBCTAL . . CANCER (HNPCC). Minorul.‘.
‘1.
KazuhfomHiromiNanasaLiz. -2. IDePartment of Dermatology, Kanazawa University School of Medicine, Japan. aDepartment of Hygiene and Oncology, Tokyo Medical and Dental University School of Medicine, Japan Defective mismatch repair underlies dte genome instability in a patient with HNICC. We carried out genetic analyses of peculii multiple keratotic turnours (KT), a keratoacanthoma (KA), and disseminated superficiaI &tic porokeratosis @SAP) developed in a patient with HNPCC. PCR ampiiication for 7 separate microsateilites reveaied prominent microsateliite instability
(MI) in 6 of 7 KTs and a KA, suggesting that replication errors
(RBR) play a role in the development of these turnours. However, PCRSSCP analysis of 2 MI+ KTs did not reveal any mutation in TGF- S type II receptor gene, the known target for RBR normally found in MI+ gastrointestinal cancers. By contrast, 2 DSAP lesions examined did not show MI. and them seemed to be no pathogenic link between DASP and RBR.
217 COMPARATIVE STUDY OF BACTEKiAL FLORA ON THE FACiAL SKIN OF ATOPIC DERMATITIS AND HEALTHY SUB.I ‘r ‘Depattmentof JECTS. m Dermatoloav. Tovama Medical and Pharmaceutical Universitv. Tovama. Jaoan. ’ Department’ of ~Laborstory Sciences, School of Health Sc~et&, Fa& of Medicine, Kanaaawa University, Kanazawa, Japan. To study the differences of characteristics of Staphylococci on the skin of stopic dermatitis and healthy subjects, the bacterial flors of these hvo groups were sampled by a contact-plate technique. The Staphylococci were selectively isolated on mannitol salt agar medium, and identified according to 20 bicchemical reactions of the API staph system. The results indicated that face of atopic dermatitis was dominantly colonized by S.oureus. The number of Staphylococci on atopic dermatitis wss higher than that on the healthy subjects. The value of M&of ten kinds of antibiotics to Staphylococci on atopic dermatitis we.m generally higher than those on healthy subjects. The susceptibility among these ten antibiotics tested, gentamytin wss the least susceptible. It was considered that increase in number of multiple resistant-Staphylococci might be seen because of much use of them. Though the correlation between the degree of rash and skin flora wss observed, it was also necessary to study the correlation between skin surface lipids and skin flora.
218 RESULTS OF PATCH TESTING With SYNTHETIC PRIMIN. M. Suaiura‘& Havakawa.Dapadment of Dermatology, Daiko Medical Canter, Nagoya Univenlty Hospital. Nagoya, Japan. Ptimin was synthesized horn 3,4dihydro-2H-pyran and guaiacol according to L.W. Siebeh method and has been dktributed among members of the Japanese Society for Contact Dermatitll. We studied whether positive reaction to synthetic prknin is relevant to pdmuia dermatitis. Using Finn Chamber and Scanportape. we carried out 48h closed patch testing with synthetic primin 0.01 %pet on 287 cases over 13 month period from February, 1906 to March 1997. Readings were mede at 24h aaer removal according to iCDRG recommendations. Two cases with @mule dermatitis end one case having past hloty of primula denatitll showed + + reactions, and 1 case with neither pdmuia denatll nor past history of primula dermatitis showed + reaction. We considered that + reaction might bs irritant reaction. Among subjects with -and +? reactions there were no cases with primula dermatitll nor with past history of primula dermatitis. We conciuciad that synthetic pnmin b asuitable allergen for patch testing and that optimum concentration is O.Ol%pet.
219 STUDY FOR SKIN TFlSTlNCi M Dm CAUSATIVE AGENTS OF OCCUPATIONAL. CONTACT DEMMATITIS. Z. Xie*l. R. &&a&-M hgis& I lkprtmmt of Hygiene. Nagoya University School of Medicine, Nagoya, Japan. 2 Ikwlmmt of Dertnatolosy,. -. Datka Medical Center, Nagoya University X&pitai. Nat&a. Jspsa Om a 2 .war .mid from Jsnasrv 1995 to Daeatber 19%. 66 oattents 121 males and 45 t&males) wclc dlagnoscd as having cccupationai dermatitis based on their wxpstionsi capones histories, onset sltustion. clinical featuresand the results of skin testing such ss dd pteh testing qm tesdng and scratch testlaa. The pstlents ccmprised 21 bmutkkns and a badxr, 13 Bctoty uorkers. 6 dental techuiclaas and s dentists, 7 agriculture-related workers, 4 cooks, 4 ““rms. and 9 others. The most beqtmtly involved stes was hands and formrats st 69.7% (46/66). Fatty six ca~epwe-c diagnosed as allergic contact demmtilis (ACD) , I I cases as irtitsnt contact dermstitb (ICD), 6 csms ss centsct vrticsria (CU). and 3 csses as CU -paaiai by ACD. ‘Ib+re was no significant dlffmce in atopic predlsumitlon rario bshveen ACD and ICD. The causstivc -&Us were dcimnkd in 62 (93.9%) cut of 66 mses: 20 (W.%) of lhe 22 barbx and beauliciam 13 WM%~ of the f&&w wrkers. 5 (71.4%) of the 7 dentist and ddd tefhttician~. ali (im~~~gticdltwweiakd~0rke~ail 4'(1oos)~00k~. all 4 (100%) nurses, and all 9 (lOti%) others. We mncludui that skin testing is sadid for
determining the causative sgcnt.8ofwork-related coatact dermstltis.
139
Abstracts
220 INDUCTION OF SUBCUTANEOUS TISSUE FIBROSIS IN NEWBORN MICE BY SIMULTANEOUS APPLICA7”3N OF TRANSFORMING GROWTH FACTOR p AND BASIC FIBRCfHLAST GROWTH FACTOR M. Shinozaki , S. Kawara, K. Takehara Department of Dermatology, Faculty of Medicine, Kanazawa University. To establish an appropriate animal model of skin fibrosis by exogenous application of growth factors, we have investigated the in viva effects of subcutaneous coinjection of TGF-S and other growth factors such as b-FGF, PDGF, and BGF in new born mice. Striking induction of granulation tissue and fibrosis was observed at the injection site after 3 consecutive days or 7 consecutive days coinjection of TGF-R and b-FGF compared with that of injected with TGF-p alone. Further, this change Persisted for
at least 2 weeks. Thus, we succeeded in establishing an animal model of skin fibrotic
disorders.
221 DETECTION OF SPECtFlC ANTIBODIES TO VASCULAR ENDOTHELIAL CELLS IN THE SERA OF PATIENTS WITH BEHCET’S DISEASE.& S. Cha’. D. Bang’. E.-S. Lee’, S. Lee’. K. H. Lee’. ‘Department of Dermatology, Yonsei Unhrersity College of Medldne, Seoul, Korea. ‘Department oi Dermatology, A]ou University Schoolof Medicine. Suwon, Korea. Autoantibodies that bind to endothelial ceils have been identlli in patlents with several forms of vascuiHls. We have detected clrculatlng antibodies to cultured human dermai mlcmvascular endothelial ceiis(HDMEC) in the sera of Behcet’s disease(BD) and other vascutar disorders including systemic lupus eryihematosus(SLE) by ELISA, SDS-PAGE and Western Molting and have observed the difference in activity of antibxiv fmm BD cOmDared with that fmm other vascular disorders. IgM antibody reacted with HDMEC surface antigen in the patients wah ED. In SLE, both IgG and IgM antibodies reacted with endothellal cell surface antlgens. in Western biotting. IgG-posiiive BD serum reacted with high molecular weighi antigen greater than 100kDa. IgG-poslthre SLE serum reacted with two omteln bands of 3CtkDa and S&Da. BD setum wntalnlng anti-endolheiial cell igM antibody reacted with 44kDa and SLE serum with 61 kDa. In dot blotting and ELISA using 44kDa HDMEC surface antigen, oniy IgM-positive BD sera reacted with the amlgen. The resuiis indicate that there are anti-endothelial cell IgM antibodies in the sera of BD, reacting with 44kDa endothelial cell surface antigen and suggest that vaacullils in BD may be caused by vascular damage due to specillc antibodies against endotheiial cells.
222 GENETIC ANALYSES OF SKIN TUMOURS DEVELOPED IN A PATIENT WTTH HEREDITARY NONPOLYPOSIS COLORBCTAL . . CANCER (HNPCC). Minorul.‘.
‘1.
KazuhfomHiromiNanasaLiz. -2. IDePartment of Dermatology, Kanazawa University School of Medicine, Japan. aDepartment of Hygiene and Oncology, Tokyo Medical and Dental University School of Medicine, Japan Defective mismatch repair underlies dte genome instability in a patient with HNICC. We carried out genetic analyses of peculii multiple keratotic turnours (KT), a keratoacanthoma (KA), and disseminated superficiaI &tic porokeratosis @SAP) developed in a patient with HNPCC. PCR ampiiication for 7 separate microsateilites reveaied prominent microsateliite instability
(MI) in 6 of 7 KTs and a KA, suggesting that replication errors
(RBR) play a role in the development of these turnours. However, PCRSSCP analysis of 2 MI+ KTs did not reveal any mutation in TGF- S type II receptor gene, the known target for RBR normally found in MI+ gastrointestinal cancers. By contrast, 2 DSAP lesions examined did not show MI. and them seemed to be no pathogenic link between DASP and RBR.