- Email: [email protected]
22phox participate in the cisplatin resistance in oral carcinoma
E-Poster Presentation Conclusion: This study demonstrates no significant correlation between peri-operative inotrope use and adverse flap outcomes. This opens the possibility for less limited use of inotropes in FTT surgery. http://dx.doi.org/10.1016/j.ijom.2015.08.051 22phox participate in the cisplatin resistance in oral carcinoma W.F. Chiang 1,∗ , J. Chen 2 , C.H. Chang 3 , S.Y. Liu 1 1
Department of Dentistry, Chi-Mei Medical Center, Liouying, Taiwan 2 Department of Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan 3 Department of Dentistry, Chang Gung Memorial Hospital, Kaohsiung, Taiwan
Background: Cisplatin (CDDP) is a well-known chemotherapeutic agent but resistance to the drug remains a major challenge in cancer treatment. Oxidative stress is the one of most important mechanisms involved in cisplatin toxicity. NADPH oxidases (NOX) are a major source of reactive oxygen species production. NOX can regulate diverse physiological processes including cell proliferation, differentiation and death. p22phox is one of the regulatory proteins whose major function is to stabilize the NOX to which it binds. Objectives: p22phox, the major modulator for the activation of NADPH oxidases, might play a role in CDDP resistance of oral cancer. Methods: RNA interference was used to knockdown p22phox and MTT assay was used to test the CDDP sensitive in oral carcinoma cell lines. p22phox distribution in tumour specimen was examined by immunohistochemistry assay. Findings: Knockdown of p22phox sensitized OSCC cell lines to CDDP (P < 0.05). Stable overexpression of p22phox augmented CDDP resistance, as evidenced by the significantly higher IC50 values. To evaluate the relation between p22phox expression and CDDP resistance, we found that p22phox was highly expressed in CDDP-resistant OSCC specimens especially the perinuclear location that is consistent with the in vivo studies. Conclusions: Our preliminary work has suggested a novel biomarker to explain the CDDP resistance in oral carcinoma. It deserved to insight into the mechanism of CDDP resistance in the further. http://dx.doi.org/10.1016/j.ijom.2015.08.052 Immediate loading on the site after cemento-osseous dysplasia was removed D. Choi ∗ , J. Park University of Hallym, Seoul, Republic of Korea Background: Now-a-days immediate loading of dental implant is one of a common process to reduce the treatment time, eliminate second surgery, reduce patient discomfortness and visiting time. But still now it has some risk of failure especially on the low quality bone. So for doing immediate loading, case selection is most important for achieving good results. To reduce the risks of failure, operators usually check the bone quality (soundness, initial stability, insertion torque, etc.)
e199
Methods: Although it must be done so carefully, .many authors reports the successful result about the immediate placement and immediate loading on infected site. In this case, the patient came with the symptoms of pain, swelling, and numbness on left mandible, and diagnosed as Cemento-Osseous Dysplasia. Buccal part of mandible was separated and re-unioned after tumour had removed. After healing, bone graft (Bio-oss) was done to gain more bone for implant but because of infection, most of them were lost remaining very thin ridge. Implant was inserted when all the infected sign was gone. Three implant of 3.5 mm diameter was inserted (Prima, Key Stone Dental). Temporary crown with acrylic resin was seated at the same day. Conclusions: Patient fills comfortable with all the procedure with good results. Final impression for prosthodontic was taken after 5 weeks. The patient feels good with no complications. http://dx.doi.org/10.1016/j.ijom.2015.08.053 Effect of the structural change of the implant neck area on the stress distribution to the surrounding tissues and the stability of the implant D. Choi ∗ , J. Park University of Hallym, Seoul, Republic of Korea Background: Alveolar bone resorption after implant occurs by several reasons, over load due to premature or uncontrolled occlusion, infection, surgical trauma, implant neck design, surgical trauma, etc. Neck design of implant is very important factors on the crestal bone loss around implant. Infection control is done by biological width Methods and findings: We tried to change implant neck design. Make wing around implant neck with different length, from 0.1 mm to 1.0 mm. And make FEM (finite element method) model to analyze force distribution around implant bone contact area. FEM model was designed as cortical bone cancellous bone and implant. Measurement was done by stress, displacement, and data was analysis with Tetra Element and Prism Element with 13236 nodal point and 62902 element, and 3D Solid structure, Linear static was analyzed. Animal study was done for test bone formation ability of wing type implant. 8 New Zealand white rabbit (2.5 kg) was used for test. Drilled on both side of tibia and implant was inserted. Upper 4 mm on drilled hole was widened 1mm both side for the sake of avoid direct contact between implant and surrounding bone. Wing type implant, with 1mm wing and conventional implant was inserted randomly. After 4weeks rabbits were sacrificed. Removal torque and histologic test were done. Wing type implant shows stress concentrated on crestal bone around wing and less stress on other area. It also shows less horizontal, vertical displacement by the horizontal and vertical force generated by occlusal force. It also shows more removal torque and more bone-to-implant contact. Conclusions: For the conclusion, wing type implant may do very positive role on the implant stability especially in week bone. http://dx.doi.org/10.1016/j.ijom.2015.08.054
Department of Dentistry, Chi-Mei Medical Center, Liouying, Taiwan 2 Department of Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan 3 Department of Dentistry, Chang Gung Memorial Hospital, Kaohsiung, Taiwan
Background: Cisplatin (CDDP) is a well-known chemotherapeutic agent but resistance to the drug remains a major challenge in cancer treatment. Oxidative stress is the one of most important mechanisms involved in cisplatin toxicity. NADPH oxidases (NOX) are a major source of reactive oxygen species production. NOX can regulate diverse physiological processes including cell proliferation, differentiation and death. p22phox is one of the regulatory proteins whose major function is to stabilize the NOX to which it binds. Objectives: p22phox, the major modulator for the activation of NADPH oxidases, might play a role in CDDP resistance of oral cancer. Methods: RNA interference was used to knockdown p22phox and MTT assay was used to test the CDDP sensitive in oral carcinoma cell lines. p22phox distribution in tumour specimen was examined by immunohistochemistry assay. Findings: Knockdown of p22phox sensitized OSCC cell lines to CDDP (P < 0.05). Stable overexpression of p22phox augmented CDDP resistance, as evidenced by the significantly higher IC50 values. To evaluate the relation between p22phox expression and CDDP resistance, we found that p22phox was highly expressed in CDDP-resistant OSCC specimens especially the perinuclear location that is consistent with the in vivo studies. Conclusions: Our preliminary work has suggested a novel biomarker to explain the CDDP resistance in oral carcinoma. It deserved to insight into the mechanism of CDDP resistance in the further. http://dx.doi.org/10.1016/j.ijom.2015.08.052 Immediate loading on the site after cemento-osseous dysplasia was removed D. Choi ∗ , J. Park University of Hallym, Seoul, Republic of Korea Background: Now-a-days immediate loading of dental implant is one of a common process to reduce the treatment time, eliminate second surgery, reduce patient discomfortness and visiting time. But still now it has some risk of failure especially on the low quality bone. So for doing immediate loading, case selection is most important for achieving good results. To reduce the risks of failure, operators usually check the bone quality (soundness, initial stability, insertion torque, etc.)
e199
Methods: Although it must be done so carefully, .many authors reports the successful result about the immediate placement and immediate loading on infected site. In this case, the patient came with the symptoms of pain, swelling, and numbness on left mandible, and diagnosed as Cemento-Osseous Dysplasia. Buccal part of mandible was separated and re-unioned after tumour had removed. After healing, bone graft (Bio-oss) was done to gain more bone for implant but because of infection, most of them were lost remaining very thin ridge. Implant was inserted when all the infected sign was gone. Three implant of 3.5 mm diameter was inserted (Prima, Key Stone Dental). Temporary crown with acrylic resin was seated at the same day. Conclusions: Patient fills comfortable with all the procedure with good results. Final impression for prosthodontic was taken after 5 weeks. The patient feels good with no complications. http://dx.doi.org/10.1016/j.ijom.2015.08.053 Effect of the structural change of the implant neck area on the stress distribution to the surrounding tissues and the stability of the implant D. Choi ∗ , J. Park University of Hallym, Seoul, Republic of Korea Background: Alveolar bone resorption after implant occurs by several reasons, over load due to premature or uncontrolled occlusion, infection, surgical trauma, implant neck design, surgical trauma, etc. Neck design of implant is very important factors on the crestal bone loss around implant. Infection control is done by biological width Methods and findings: We tried to change implant neck design. Make wing around implant neck with different length, from 0.1 mm to 1.0 mm. And make FEM (finite element method) model to analyze force distribution around implant bone contact area. FEM model was designed as cortical bone cancellous bone and implant. Measurement was done by stress, displacement, and data was analysis with Tetra Element and Prism Element with 13236 nodal point and 62902 element, and 3D Solid structure, Linear static was analyzed. Animal study was done for test bone formation ability of wing type implant. 8 New Zealand white rabbit (2.5 kg) was used for test. Drilled on both side of tibia and implant was inserted. Upper 4 mm on drilled hole was widened 1mm both side for the sake of avoid direct contact between implant and surrounding bone. Wing type implant, with 1mm wing and conventional implant was inserted randomly. After 4weeks rabbits were sacrificed. Removal torque and histologic test were done. Wing type implant shows stress concentrated on crestal bone around wing and less stress on other area. It also shows less horizontal, vertical displacement by the horizontal and vertical force generated by occlusal force. It also shows more removal torque and more bone-to-implant contact. Conclusions: For the conclusion, wing type implant may do very positive role on the implant stability especially in week bone. http://dx.doi.org/10.1016/j.ijom.2015.08.054