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237 Estradiol receptor concentrations and estradiol dehydrogenase activity in the endometrium of finnish and Japanese women
79s
236
237
THE SIGNIFICANCE
OF STEROID HORMONE CONCENTRATIONS IN ENDOMETRIUM AND PLASMA FROM THE RELATIONSHIP TO OESTRADIOL METABOLISM. CANCER PATIENTS AND POSTMENOPAUSAL WOMEN; Bonney, R.C., Scanlon, M.J., Jones, D.L., Reed, M.J. & James, V-H-T. Department of Chemical Pathology, St. Mary's Hospital Medical School, London W2 lPG, U.K. It has been proposed that adrenal androgens are involved in the development of endometrial cancer either by an oestrogenic action at the receptor level or through inAttempts to define this role have centred on plasma hibition of oestradiol metabolism. measurements rather than on androgen content of the endometrium itself. Here we report (DHA) and concentrations of 5-androstene-3P,17P-diol (ADIOL), dehydroepiandrosterone its sulphate (DHAS), oestrogens (El,E2) and progesterone (P) in endometrium and plasma from cancer patients (49-81 years) and normal postmenopausal women (49-68 years). Similar mean steroid concentrations were found in both plasma and tissue for both groups of subjects, i.e. plasma ADIOL fpgfml + SD, n=lO): cancer patients, 342.9 + 174.8, normal women, 343.3 + 207.1 and tissue ADIOL (rig/g 2 SDkcancer patients, 1.93 In malignant tissue,correlations were + 2.18 (n=13), normal women,- 1.34 + 1.17 (n=8). Tound for ADIOL v DHA, El v E2 and-P v DHAS and in normal tissue for ADIOL v DHAS and DHA Y DHAS. Correlations between all 3 androgens and for El v E2 were found in plasma from cancer patients qnd for ADIOL v DHAS and DHA v P in plasma from normal subjects. There was no relationship between E2->El conversion and tissue steroid content. These data could suggest an imbalance of sulphatase and sulphotransferase activity in malignant endometrium and a possible involvement of P in promoting this imbalance.
ESTRADIOL RECEPTOR CONCENTRATIOKS AND ESTRADIClL OEtlYDRUGENASE ACTIVITY IN THE ENDOMETRIM? @F FINNISH AND JAPANESE WUb:EN. R.Punnonen, A.Lukola and R.Kudo,Dept Obstet Gynec, Univ of Tampere; Dept Biochem, iibo Akadeand Dept Obstet Gynec, Sapporo T"ed College, Japan Turku, Finland, mi, The
connection
between.estrogens
and
endometrial
carcinoma
has
Since the endometrial carcinoma reported in numerous studies. we studied the endometrial more common in Finland than in Japan, gen metabolism in Finnish and Japanese women.
been much estro-
is
The endometrial specimens were taken in connection with hysterectomy All the women were menstruating from 13 Finnish and 20 Japanese women, and their age was between 40 and 50. In both groups the endometrium specimens were placed in liquid nitrogen immediately after the hysterectomy and stored until determination of the estradiol cytosol receptors and estradiol dehydroqenase activity. The estradiol endometrium was (p
cytosoi 245.9f46.2
Ll.05).
receptor concentration in the proliferative in Finnish and 45.7k17.1 in Jaoanese women endometrium the corresponding numbers were
In secretory and 20.0f6.4 respectively (p U.05). No differences were found in estradiol dehydrogenase activity between follicular and luteal phases of the cycle in Finnish or Japanese women.
97.8k33.7
238
ANALYSIS
OF CYTOSOL RECEPTOR CONCENTRATIONS (ER,PR)
Lars-GGsta Gothenburg,
Friberg, Sweden.
Anita
Nilsson,
King Gustav
IN ENDOMETRIAL CANCER.
V Jubileumsklinik,
Sahlgren
Hospital,
ER and PR in 175 cases of nontreated endometrial cancer stage I-II are plotted connected,correlated to differentiation grade using DCC-technique and Scatchard method. Pos. values > 3 fmoles/mg protein results in about 10% negative cases, A strong correlation exists between low values and low diff as well as between high values and high diff. In low values ER has the highest figure and in high values PR are highest. ER is ;nostly pos. in low diff when PR is neg. ER keeps to a level of Z-300 although PR in high diff reach 1-2000 fmoles~ng protein. Anti-oestrogen given before treatment gives an increased PR in low diff but no change in ER. Samples taken before and after intracavitary treatment show a decrease in ER and PA to low values or zero.
MAIN TOPIC Author: 7,: .,L ,
7: Gynecological Cancer
Lare43sta Fribexg MD,King Guetav V Jubileumklinik, Sahlgren Hospital 413 45 Gothenburg Sweden
236
237
THE SIGNIFICANCE
OF STEROID HORMONE CONCENTRATIONS IN ENDOMETRIUM AND PLASMA FROM THE RELATIONSHIP TO OESTRADIOL METABOLISM. CANCER PATIENTS AND POSTMENOPAUSAL WOMEN; Bonney, R.C., Scanlon, M.J., Jones, D.L., Reed, M.J. & James, V-H-T. Department of Chemical Pathology, St. Mary's Hospital Medical School, London W2 lPG, U.K. It has been proposed that adrenal androgens are involved in the development of endometrial cancer either by an oestrogenic action at the receptor level or through inAttempts to define this role have centred on plasma hibition of oestradiol metabolism. measurements rather than on androgen content of the endometrium itself. Here we report (DHA) and concentrations of 5-androstene-3P,17P-diol (ADIOL), dehydroepiandrosterone its sulphate (DHAS), oestrogens (El,E2) and progesterone (P) in endometrium and plasma from cancer patients (49-81 years) and normal postmenopausal women (49-68 years). Similar mean steroid concentrations were found in both plasma and tissue for both groups of subjects, i.e. plasma ADIOL fpgfml + SD, n=lO): cancer patients, 342.9 + 174.8, normal women, 343.3 + 207.1 and tissue ADIOL (rig/g 2 SDkcancer patients, 1.93 In malignant tissue,correlations were + 2.18 (n=13), normal women,- 1.34 + 1.17 (n=8). Tound for ADIOL v DHA, El v E2 and-P v DHAS and in normal tissue for ADIOL v DHAS and DHA Y DHAS. Correlations between all 3 androgens and for El v E2 were found in plasma from cancer patients qnd for ADIOL v DHAS and DHA v P in plasma from normal subjects. There was no relationship between E2->El conversion and tissue steroid content. These data could suggest an imbalance of sulphatase and sulphotransferase activity in malignant endometrium and a possible involvement of P in promoting this imbalance.
ESTRADIOL RECEPTOR CONCENTRATIOKS AND ESTRADIClL OEtlYDRUGENASE ACTIVITY IN THE ENDOMETRIM? @F FINNISH AND JAPANESE WUb:EN. R.Punnonen, A.Lukola and R.Kudo,Dept Obstet Gynec, Univ of Tampere; Dept Biochem, iibo Akadeand Dept Obstet Gynec, Sapporo T"ed College, Japan Turku, Finland, mi, The
connection
between.estrogens
and
endometrial
carcinoma
has
Since the endometrial carcinoma reported in numerous studies. we studied the endometrial more common in Finland than in Japan, gen metabolism in Finnish and Japanese women.
been much estro-
is
The endometrial specimens were taken in connection with hysterectomy All the women were menstruating from 13 Finnish and 20 Japanese women, and their age was between 40 and 50. In both groups the endometrium specimens were placed in liquid nitrogen immediately after the hysterectomy and stored until determination of the estradiol cytosol receptors and estradiol dehydroqenase activity. The estradiol endometrium was (p
cytosoi 245.9f46.2
Ll.05).
receptor concentration in the proliferative in Finnish and 45.7k17.1 in Jaoanese women endometrium the corresponding numbers were
In secretory and 20.0f6.4 respectively (p U.05). No differences were found in estradiol dehydrogenase activity between follicular and luteal phases of the cycle in Finnish or Japanese women.
97.8k33.7
238
ANALYSIS
OF CYTOSOL RECEPTOR CONCENTRATIONS (ER,PR)
Lars-GGsta Gothenburg,
Friberg, Sweden.
Anita
Nilsson,
King Gustav
IN ENDOMETRIAL CANCER.
V Jubileumsklinik,
Sahlgren
Hospital,
ER and PR in 175 cases of nontreated endometrial cancer stage I-II are plotted connected,correlated to differentiation grade using DCC-technique and Scatchard method. Pos. values > 3 fmoles/mg protein results in about 10% negative cases, A strong correlation exists between low values and low diff as well as between high values and high diff. In low values ER has the highest figure and in high values PR are highest. ER is ;nostly pos. in low diff when PR is neg. ER keeps to a level of Z-300 although PR in high diff reach 1-2000 fmoles~ng protein. Anti-oestrogen given before treatment gives an increased PR in low diff but no change in ER. Samples taken before and after intracavitary treatment show a decrease in ER and PA to low values or zero.
MAIN TOPIC Author: 7,: .,L ,
7: Gynecological Cancer
Lare43sta Fribexg MD,King Guetav V Jubileumklinik, Sahlgren Hospital 413 45 Gothenburg Sweden