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244 Inhibition of allergen-induced bronchoconstrictive reaction in sensitized guinea pigs by orally administered allergen
VOLUME NUMBER
241
Abstracts
87 1. PART 2
REDUCTION OF SIDE EFFECTS OF BEE VENOM IMMUNOTHERAPY BY PRETREATMENTWITH TERFENADINE. E. Berchtold, M.D., R. Maibach, Ph.D. and U. Miiller, M.D., Bern, Switzerland
243
The purpose of this study was to evaluate prophylactic treatment with Hl-antihistamines for prevention of allergic side effects (SE) due to honey bee venom immunotherapy (BVIT). Methods: In a double-blind placebo controlled trial 52 patients with a history of severe systemic reactions to honey bee stings and positive skin tests and RAST to bee venom (BV) were submitted to rush-BVIT with 4 daily injections up to a maintenance dose of 100 ug, which was usually reached after four days. Patients were randomly assigned to pretreatment with Terfenadine 120 mg bid or placebo. During rush-BVIT local reactions, all systemic SE and consumption of additional anti-allergic medication were recorded. Results: Under pretreatment with Terfenndine local reactions (p 4 0.05) and consumption of anti-allergic medication (p (0.03) was significantly reduced. The total incidence of systemic SE was not significantly lower in patients on Terfenadine. Analysis of individual allergic symptoms, however, showed significant reduction of generalized itching (p < 0.006) and urticaria/angio-oedema (p< 0.03) but not of respiratory (cough, wheezing) or cardiovascular symptoms (flush, dizziness) in the Terfenadine-treated group. Conclusions: Pretreatment with Terfenadine significantly reduced local reactions and cutaneous SE of rush-BVIT, but had no influence on respiratory or cardiovascular SE.
242
I-: @ AL~TIVETREATXEZIT @ ALLZZXXC DIVITIS. J- Cuesta, M.D., F'h.D., A. Blanco MD, F'hD.. & Cue&a. AL MD PuYaM, MD., g Alvarezcuesta, M.D., u Hospital Ran y Cajal. Madrid Spain Allergy to cats was selected as the model for the double blind placebo controlled study to evaluate umunotherapy In the treatment of allergic conjunctlvltls. 28 patients particrpated, 14 of whom received biologically standardized cat extract quantified with rnonoclonal antlbodles (ABELLO S.A. 1. A symptommedication score (SS), conJunctiva1 provocation test (CPT) and slcln reactivity with the prick-test using cat extract (200, 4Oand 8 Fe1 d I (33 ug/l@.? Bu), end point @J/ml ), tltulatlon (EFT) and parallel line assay were done. The Mann-Wlutney and (PM) 1 Wllcoxon Tests was used for the statistical analysrs. R. : At 12 months of treatment, the fOllOgroup were seen: Wing changes in the active decrease in the SMS (p
PI1DwcIIoM EOGINOPHIL OF ~~~ CATIONIC PRDTEIN (ECP) AFTEX? succEssFuL UMJNUEEHAPY Chi-Chang Shieh, M.D., and Kue-Hsiung Hsieh, M.D. Taipei, Taiwan, l&x. Eosinophil &ionic protein (ECP) is one of the cytotoxic proteins secreted fmn eosinophil leukocytes during allergic reactions. In order to sttiythe role of ECP in bronchial astlrna in children andtheworking mechanism of inrmmotherapy (IT), bloods were sampled from 10 each of new patients, gocd responders to IT, poor responders to IT, and IxIITM-1 controls. Total eosinophil counts (TX) were enurerated with eosin stain and senm level of ECP were quantitat&, using FCP RIA kit (Phannscia, Swed.en). Our results demonstrated that the new patients had higher rerun ECP levels than nonral controls (pZO.05). After IT, while serun ECP tended to decrease in m responders (1.94+2.15 for new A.4g/l patients, 1.12+i.63 for gccd W/l responders, pt0.i). serunECPwas increased in poor responders (1.94+2.15 Aq/l vs 3.5t1.13 &g/l, ptO.05). T&re was a signif~cant difference inBCP between poor and goal responders (3.5OLl.13 ug/l vs 1.12~1.23 The TEC was3 396+208/mn , W/l. Pq.05). and 688+367/m , 884+574/nni for gwd r&onders, new patients-a poor responders, respectively. There was no correlation betweenTl?C andECP. We therefore concltiethatdecreased DCP nay account partly for the clinical efficacy of imnunotherapy andmeasur~ntof
!ZCP rosy pruvideapara-wter response to immmotherapy.
244
199
to
judge
the
INHIBITION OF ALLERGEN-INDUCED BKONCHOCONSTKICTIVE BEAGTIGN IN SENSITIZED GUINEA PIGS BY OKALLY ADMINISTE:RED ALLKRGBN.A.Ishii.M.D.,Y.Ino,Ph.D. M.Haida,M.D.,M.Suko,M.D.,Y.Horita,M.D.,K.Ito,M.D. H.Okudaira,M.D.,Tokyo,Japan Present study was carried out to evaluate the effect of oral administration of the crude mite extract(CME) on mite sensitized guinea pigs on their bronchoconstrictive reactions induced by inhalation of the CME. Male Hartley guinea pigs were immunized with the CMB according to the method of Grasiano. All animals presented positive reaction when given inhalation provocation tests.l'he airway hypersensitivity was measured by PC200,denoted as the threshold concentration of CME necessary to double the respiratory resistance.The animals were then divided into 4 groups one of which was fed only tap water for control.The other 3groups were fed either 1,lO or lOOmg/day of CME for 10 days.Inhalation provocation tests were carried out 1 day before(2nd),and lOdays(3rd) and 17days (4th) after the withdrawal of the oral CMK administration.Other tests such as the determination of the levels of serum IgK,IgGl,IgGZ antibodies, histamine provocation tests,Schults-Dale reaction and the histamine release from the chopped lung fragments were also carried out. Significant rise in the threshold concentration was observed in the 2nd and the 3rd provocation tests in all groups that were fed the Cede which persisted for about 10 days after the cessation of the oral administration of the CMK. The results obtained suggested that the reduced reactivity of the bronchial tissues may be due to the decrease in the bronchial smooth muscle itself.
241
Abstracts
87 1. PART 2
REDUCTION OF SIDE EFFECTS OF BEE VENOM IMMUNOTHERAPY BY PRETREATMENTWITH TERFENADINE. E. Berchtold, M.D., R. Maibach, Ph.D. and U. Miiller, M.D., Bern, Switzerland
243
The purpose of this study was to evaluate prophylactic treatment with Hl-antihistamines for prevention of allergic side effects (SE) due to honey bee venom immunotherapy (BVIT). Methods: In a double-blind placebo controlled trial 52 patients with a history of severe systemic reactions to honey bee stings and positive skin tests and RAST to bee venom (BV) were submitted to rush-BVIT with 4 daily injections up to a maintenance dose of 100 ug, which was usually reached after four days. Patients were randomly assigned to pretreatment with Terfenadine 120 mg bid or placebo. During rush-BVIT local reactions, all systemic SE and consumption of additional anti-allergic medication were recorded. Results: Under pretreatment with Terfenndine local reactions (p 4 0.05) and consumption of anti-allergic medication (p (0.03) was significantly reduced. The total incidence of systemic SE was not significantly lower in patients on Terfenadine. Analysis of individual allergic symptoms, however, showed significant reduction of generalized itching (p < 0.006) and urticaria/angio-oedema (p< 0.03) but not of respiratory (cough, wheezing) or cardiovascular symptoms (flush, dizziness) in the Terfenadine-treated group. Conclusions: Pretreatment with Terfenadine significantly reduced local reactions and cutaneous SE of rush-BVIT, but had no influence on respiratory or cardiovascular SE.
242
I-: @ AL~TIVETREATXEZIT @ ALLZZXXC DIVITIS. J- Cuesta, M.D., F'h.D., A. Blanco MD, F'hD.. & Cue&a. AL MD PuYaM, MD., g Alvarezcuesta, M.D., u Hospital Ran y Cajal. Madrid Spain Allergy to cats was selected as the model for the double blind placebo controlled study to evaluate umunotherapy In the treatment of allergic conjunctlvltls. 28 patients particrpated, 14 of whom received biologically standardized cat extract quantified with rnonoclonal antlbodles (ABELLO S.A. 1. A symptommedication score (SS), conJunctiva1 provocation test (CPT) and slcln reactivity with the prick-test using cat extract (200, 4Oand 8 Fe1 d I (33 ug/l@.? Bu), end point @J/ml ), tltulatlon (EFT) and parallel line assay were done. The Mann-Wlutney and (PM) 1 Wllcoxon Tests was used for the statistical analysrs. R. : At 12 months of treatment, the fOllOgroup were seen: Wing changes in the active decrease in the SMS (p
PI1DwcIIoM EOGINOPHIL OF ~~~ CATIONIC PRDTEIN (ECP) AFTEX? succEssFuL UMJNUEEHAPY Chi-Chang Shieh, M.D., and Kue-Hsiung Hsieh, M.D. Taipei, Taiwan, l&x. Eosinophil &ionic protein (ECP) is one of the cytotoxic proteins secreted fmn eosinophil leukocytes during allergic reactions. In order to sttiythe role of ECP in bronchial astlrna in children andtheworking mechanism of inrmmotherapy (IT), bloods were sampled from 10 each of new patients, gocd responders to IT, poor responders to IT, and IxIITM-1 controls. Total eosinophil counts (TX) were enurerated with eosin stain and senm level of ECP were quantitat&, using FCP RIA kit (Phannscia, Swed.en). Our results demonstrated that the new patients had higher rerun ECP levels than nonral controls (pZO.05). After IT, while serun ECP tended to decrease in m responders (1.94+2.15 for new A.4g/l patients, 1.12+i.63 for gccd W/l responders, pt0.i). serunECPwas increased in poor responders (1.94+2.15 Aq/l vs 3.5t1.13 &g/l, ptO.05). T&re was a signif~cant difference inBCP between poor and goal responders (3.5OLl.13 ug/l vs 1.12~1.23 The TEC was3 396+208/mn , W/l. Pq.05). and 688+367/m , 884+574/nni for gwd r&onders, new patients-a poor responders, respectively. There was no correlation betweenTl?C andECP. We therefore concltiethatdecreased DCP nay account partly for the clinical efficacy of imnunotherapy andmeasur~ntof
!ZCP rosy pruvideapara-wter response to immmotherapy.
244
199
to
judge
the
INHIBITION OF ALLERGEN-INDUCED BKONCHOCONSTKICTIVE BEAGTIGN IN SENSITIZED GUINEA PIGS BY OKALLY ADMINISTE:RED ALLKRGBN.A.Ishii.M.D.,Y.Ino,Ph.D. M.Haida,M.D.,M.Suko,M.D.,Y.Horita,M.D.,K.Ito,M.D. H.Okudaira,M.D.,Tokyo,Japan Present study was carried out to evaluate the effect of oral administration of the crude mite extract(CME) on mite sensitized guinea pigs on their bronchoconstrictive reactions induced by inhalation of the CME. Male Hartley guinea pigs were immunized with the CMB according to the method of Grasiano. All animals presented positive reaction when given inhalation provocation tests.l'he airway hypersensitivity was measured by PC200,denoted as the threshold concentration of CME necessary to double the respiratory resistance.The animals were then divided into 4 groups one of which was fed only tap water for control.The other 3groups were fed either 1,lO or lOOmg/day of CME for 10 days.Inhalation provocation tests were carried out 1 day before(2nd),and lOdays(3rd) and 17days (4th) after the withdrawal of the oral CMK administration.Other tests such as the determination of the levels of serum IgK,IgGl,IgGZ antibodies, histamine provocation tests,Schults-Dale reaction and the histamine release from the chopped lung fragments were also carried out. Significant rise in the threshold concentration was observed in the 2nd and the 3rd provocation tests in all groups that were fed the Cede which persisted for about 10 days after the cessation of the oral administration of the CMK. The results obtained suggested that the reduced reactivity of the bronchial tissues may be due to the decrease in the bronchial smooth muscle itself.