- Email: [email protected]
254. Steroidogenesia in the desensitized rat Corpora lutea(CL)
lxxxv
Abstracts 253. SUCCESSFUL TREATAENT OF THE DEFICIENT LUTEAL PHASE
J.R.T. Coutts, R. Fleming, W.P. Black and N.C. Macnaughton, Department of Obstetrics and Gynaecology, Glasgow University, United Kingdom. A number of endocrine abnormalities have been delineated in women with unexplained infertility by assessment of daily plasma hormone profiles. However, the relationship of such abnormalities to the "infertility" is not clear. Pregnancy rates in such women have in the past been disappointing no matter what form of therapy was used. By contrast, use of the appropriate treatment in patients with anovulatory ammsnorrhea has resulted in normal conception rates. Infertile women in whom a deficient lutsal phase (poor progesterone surge) had been previously shown have been successfully treated using a new approach. Hypogonadotrophichypogonadism was induced and maintained using supra-optimal doses of the LHRH analogue Hoe 766 and the women wers then treated with exogenous gonadotrophins to induce ovulation. Four of the first five women (unexplained infertility from 7 to 13 years) so treated have conceived within six treatment courses whilst the last patient dsclined further treatment after two courses. These data suggest that at least one of the endocrine abnormalitiss found in women with unsxplainsd infertility (the deficisnt lutsal phase) is a causative factor. Hoe 766 was kindly donated by Dr. S. Dombey of Hoschst U.K. Limited.
254. Steroidogenesis in the desensitized r& Corpora lutea by R.R.Dighe k N.R.Moudgal, Center for Adv.Study in Reprod.Biol., Lab.Endo.Biochem., Indim Institute of Science, Bangslore012, India. Earlier workers have ob8erved that in the leydig cell desensitization brings results in addition to down regulation of receptors, in leisons in the steroidogenlc pathway. In the present study immature rats having heavily leutinized ovaries were given 50 iu hCG End the dese sitized CL removed 48h later were used. At that time no change in thea s 30HSD activity and CAMP binding activity(a measure of CAMP dependent protein Uaase) was observed.Followlng desensitization however,l)a significent increase in phosphodiestrase activity,ii)a 50% reduction in total mitochondrial cholesterol level, iii)a significant reduction in its ability to utilize cholesterol or hydrolyse its ester d 1v)a significant lowering(by 66$)in cholesterol side chain cleqm activity(by measuring pregnanalone formed) was observed. Pregnenalone production was restored to normalcy if exogenous cholesterol was added to the mitohondrial preparation. The results suggest that luteal desensitization is due in addition to down regulation of LH receptors, to a marked reduction in available cholesterol pool in the mitochondrial compartment. The increase in phosphodiestrase activity, though probably a secondary effect,might effectively contribute to the overall reduction in the steroid out-put by increasing the catabolism of cAMP.(Aided by grants from ICMR,New Delhi and WHO, Geneva).
24. PHARMACOLOGY
AND PHARMACODYNAMICS STEROIDS
OF CONTRACEPTIVE
RECEPTORS IN INTRA-UTERINE CONTRACEPTIVE 255. ENDOMETRIAL ESTROGEN AND PROGESTERONE
DEVICEUSERS. Kaplan, B., Maayan, R., Tadir, Y., Kofman, H. and Ovadia, Y. - Department of Obstetrics and Gynecology and Endocrinology Laboratory, Beilinson Medical Center and Tel-Aviv University Sackler School of Medicine. Quantitative measurements of free estradiol and progesterone cytoplasmic receptors from human endometrium were performed in sixteen patients who had been using intrauterine contraceptive devices (I.U.C.D.) for at least two years. The samples were taken at various stages of the menstrual cycle and plasma levels of Endometrium of fourteen estradiol and progesterone were measured simultaneously. non-I.U.C.D. users served as control. The receptor levels were measured usin; a single saturating dose assay. The estradiol and progesterone receptor concentrations were highest during the proliferative phase No significant and were significantly lower in the second half of the cycle. difference was found between the I.U.C.D. users and the control group. Our findings suggest that the contraceptive effect of I.U.C.D. is not related to the hormonal receptors in the endometrial tissue, as proposed by others.
Abstracts 253. SUCCESSFUL TREATAENT OF THE DEFICIENT LUTEAL PHASE
J.R.T. Coutts, R. Fleming, W.P. Black and N.C. Macnaughton, Department of Obstetrics and Gynaecology, Glasgow University, United Kingdom. A number of endocrine abnormalities have been delineated in women with unexplained infertility by assessment of daily plasma hormone profiles. However, the relationship of such abnormalities to the "infertility" is not clear. Pregnancy rates in such women have in the past been disappointing no matter what form of therapy was used. By contrast, use of the appropriate treatment in patients with anovulatory ammsnorrhea has resulted in normal conception rates. Infertile women in whom a deficient lutsal phase (poor progesterone surge) had been previously shown have been successfully treated using a new approach. Hypogonadotrophichypogonadism was induced and maintained using supra-optimal doses of the LHRH analogue Hoe 766 and the women wers then treated with exogenous gonadotrophins to induce ovulation. Four of the first five women (unexplained infertility from 7 to 13 years) so treated have conceived within six treatment courses whilst the last patient dsclined further treatment after two courses. These data suggest that at least one of the endocrine abnormalitiss found in women with unsxplainsd infertility (the deficisnt lutsal phase) is a causative factor. Hoe 766 was kindly donated by Dr. S. Dombey of Hoschst U.K. Limited.
254. Steroidogenesis in the desensitized r& Corpora lutea by R.R.Dighe k N.R.Moudgal, Center for Adv.Study in Reprod.Biol., Lab.Endo.Biochem., Indim Institute of Science, Bangslore012, India. Earlier workers have ob8erved that in the leydig cell desensitization brings results in addition to down regulation of receptors, in leisons in the steroidogenlc pathway. In the present study immature rats having heavily leutinized ovaries were given 50 iu hCG End the dese sitized CL removed 48h later were used. At that time no change in thea s 30HSD activity and CAMP binding activity(a measure of CAMP dependent protein Uaase) was observed.Followlng desensitization however,l)a significent increase in phosphodiestrase activity,ii)a 50% reduction in total mitochondrial cholesterol level, iii)a significant reduction in its ability to utilize cholesterol or hydrolyse its ester d 1v)a significant lowering(by 66$)in cholesterol side chain cleqm activity(by measuring pregnanalone formed) was observed. Pregnenalone production was restored to normalcy if exogenous cholesterol was added to the mitohondrial preparation. The results suggest that luteal desensitization is due in addition to down regulation of LH receptors, to a marked reduction in available cholesterol pool in the mitochondrial compartment. The increase in phosphodiestrase activity, though probably a secondary effect,might effectively contribute to the overall reduction in the steroid out-put by increasing the catabolism of cAMP.(Aided by grants from ICMR,New Delhi and WHO, Geneva).
24. PHARMACOLOGY
AND PHARMACODYNAMICS STEROIDS
OF CONTRACEPTIVE
RECEPTORS IN INTRA-UTERINE CONTRACEPTIVE 255. ENDOMETRIAL ESTROGEN AND PROGESTERONE
DEVICEUSERS. Kaplan, B., Maayan, R., Tadir, Y., Kofman, H. and Ovadia, Y. - Department of Obstetrics and Gynecology and Endocrinology Laboratory, Beilinson Medical Center and Tel-Aviv University Sackler School of Medicine. Quantitative measurements of free estradiol and progesterone cytoplasmic receptors from human endometrium were performed in sixteen patients who had been using intrauterine contraceptive devices (I.U.C.D.) for at least two years. The samples were taken at various stages of the menstrual cycle and plasma levels of Endometrium of fourteen estradiol and progesterone were measured simultaneously. non-I.U.C.D. users served as control. The receptor levels were measured usin; a single saturating dose assay. The estradiol and progesterone receptor concentrations were highest during the proliferative phase No significant and were significantly lower in the second half of the cycle. difference was found between the I.U.C.D. users and the control group. Our findings suggest that the contraceptive effect of I.U.C.D. is not related to the hormonal receptors in the endometrial tissue, as proposed by others.