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256 IN VITRO TEST SYSTEM FOR THE EVALUATION OF THE BIOCOMPATIBILITY OF ALLOPLASTIC MATERIALS
Conclusions: 7KLV VWXG\ LQGLFDWHG WKDW WKH VPRRWK PXVFOH UHJHQHUDWLRQ DQG YDVFXODUL]DWLRQLQWLVVXHHQJLQHHUHGQHREODGGHUFRXOGEHHQKDQFHGE\DXWRORJRXV HQGRWKHOLDOSURJHQLWRUFHOOVDQGH[RJHQRXVELRDFWLYHIDFWRUV
256
IN VITRO TEST SYSTEM FOR THE EVALUATION OF THE BIOCOMPATIBILITY OF ALLOPLASTIC MATERIALS
(LPHU&*HUXOOLV+%DJQHU-/DPPHUV%-7KHXHUNDXI,5DPRQ$ 2WWR7&HQWHUIRU,QQRYDWLYH7HFKQLTXHVLQ0HGLFLQH 1 Lukas Hospital, Dept. of Urology, Neuss, Germany, 2Lukas Hospital, Dept. of Surgery, Neuss, Germany, 3Lukas Hospital, Dept. of Pathology, Neuss, Germany, 4 International Tissue Engineering Research Association, Itera, Bad Aachen, Germany Introduction & Objectives: $OORSODVWLFPDWHULDOVDUHZLGHO\DSSOLHGLQWKHVXUJLFDO WKHUDSLHVIRUKHUQLDLQFRQWLQHQFHDQGSURODSVHVLWXDWLRQV7KH$PHULFDQ)RRGDQG 'UXJ$GPLQLVWUDWLRQ )'$ LVVXHGDZDUQLQJFRQFHUQLQJWKHDSSOLFDWLRQRIDOORSODVWLF PDWHULDOVIRUWKHVXUJLFDOWUHDWPHQWRIIHPDOHLQFRQWLQHQFHDQGSURODSVHUHSRUWLQJ PRUHWKDQVHYHUHDGYHUVHHYHQWVDVVRFLDWHGZLWKWKHXVHGPDWHULDOV6SHFLDO VXUJLFDOWUDLQLQJWRJHWKHUZLWKWKHPDQDJHPHQWRIULVNDQG$(vVDUHUHFRPPHQGHG ZZZIGDJRYFGUKVDIHW\VXUJLFDOPHVKKWPO 7KH LPSURYHPHQW RI WKH ELRFRPSDWLELOLW\FRDWLQJIRUHLJQPDWHULDOVZLWKHJDXWRORJRXVVXEVWDQFHVVHHPV WREHPDQGDWRU\ Material & Methods: ,QRUGHUWRLQYHVWLJDWHLIFRDWLQJRIDOORSODVWLFPDWHULDOVZLWK DXWRORJRXVPDWHULDOFDQOHDGWRLPSURYHGELRFRPSDWLELOLW\DQGLIDOORSODVWLFPDWHULDOV DUHLQWHJUDWHGLQWRWKHVWUXFWXUHRIWKHSHOYLFᚐRRUWKHIROORZLQJVWXG\DSSURDFKZDV HVWDEOLVKHG SDWLHQWVZHUHLQFOXGHG0\REODVWVHQGRWKHOLDOFHOOVDQGᚏEUREODVWV RIHDFKSDWLHQWZHUHFXOWLYDWHGVHSDUDWHO\&HOOFRXQWDQGJURZWKDUHFRPSDUDEOH LQ DOO DSSURDFKHV ZLWKLQ D WHVW GXUDWLRQ RI ZHHNV ,PPXQRKLVWRFKHPLFDO DQG PLFURVFRSLF HYDOXDWLRQ RI FHOO FRXQW DQG DGKHUHQFH LV SHUIRUPHG FRQVLGHULQJ WKH FKRVHQ FRDWLQJ IRU WKH DOORSODVWLF PDWHULDO $GGLWLRQDOO\ ZH SHUIRUPHG LPPXQRF\WRFKHPLVWU\ LQ RUGHU WR YHULI\ WKH SUHVHQFH RI WKHVH FHOOV SURYLQJ WKH H[SUHVVLRQ RI VSHFLᚏF FHOO PDUNHUV 7KH GHWHFWLRQ RI P\REODVWV ZDV UHDOLVHG E\ ˞VDUFRPHULFDFWLQDQGGHVPLQDVPDUNHUVRIP\RJHQHGLᚎHUHQWLDWLRQ)LEUREODVWV ZHUHGHPRQVWUDWHGE\WKHXVHRIDQWLERGLHVWDUJHWLQJYLPHQWLQZKHUHDVDQWLERGLHV DJDLQVW&'ZHUHXWLOLVHGIRUWKHGHWHFWLRQRIHQGRWKHOLDOFHOOV$GKHUHQFHDQG FHOO FRXQW DUH DVVHVVHG PLFURVFRSLFDOO\ DQG E\ LPPXQRKLVWRFKHPLVWU\ DIWHU FR LQFXEDWLRQRIWKHFHOOVZLWKGLᚎHUHQWW\SHVRIDOORSODVWLFPHVKV Results: GLᚎHUHQWFRPPRQO\XVHGDOORSODVWLFPDWHULDOVZHUHLQYHVWLJDWHG&HOO FRXQW DQG JURZWK ZHUH FRPSDUDEOH LQ DOO DSSURDFKHV ZLWKLQ D WHVWGXUDWLRQ RI ZHHNV2QO\RQ7)70RWLIPHVKFHOOVDUHVKRZLQJDGKHUHQFHDQGJURZWK2QDOO RWKHU PHVKV WKHUH LV QR HYLGHQFH RI FHOO DGKHUHQFH WR WKH UHVSHFWLYH DOORSODVWLF PDWHULDOV7KHLQYLWURDQDO\VHVZHUHLQGHSHQGHQWIURPJHQGHUDQGLQGLYLGXDOSDWLHQW IHDWXUHV 5HJDUGLQJ WLVVXH VSHFLI\ GLᚎHUHQW FHOO JURZWK FDQ GH GHᚏQHG LQ IDYRXU RIᚏEUREODVWV ᚏEUREODVWV!P\REODVWV!HQGRWKHOLDOFHOOV,PSURYHGFHOODGKHVLRQ ZDVIRXQGRQDOORSODVWLFPHVKVFRYHUHGZLWKDXWRORJRXVSODVPDFRPSDUHGWRQRQ FRYHUHG PHVKV 7KLV ᚏQGLQJ LQFOXGHV DOO W\SHV RI FHOOV EHLQJ WHVWHG $ FRYHULQJ RI PHVKV ZLWK VHUXP RU FROODJHQ VHHPV WR EH RI GLVDGYDQWDJH /RRNLQJ DW WKH LQWHQVLW\ RI FHOO DGKHVLRQ RQ WKH GLᚎHUHQW PHVKV WKH IROORZLQJ UDQNLQJ FDQ EH GLVSRVHG 7)70RWLIPHVKp3UR[\%LRPHGLFDO'\QDPHVKp)(*7H[WLOWHFKQLN 6XUJLPHVKp $VSLGH 0HGLFDO 5HVRUED 3'6 3ODWHp -RKQVRQ -RKQVRQ 0HUVLOHQH%DQG-RKQVRQ -RKQVRQ9LWDPHVKp3UR[\%LRPHGLFDO9LFU\O%DQG -RKQVRQ -RKQVRQ 7L/RRSp 3)0&HOO DGKHVLRQ RQ WKH IROORZLQJ DOORSODVWLF PDWHULDOVLVQRWVHHQQHLWKHULQWKHXQFRYHUHGQRULQWKHFRYHUHGDSSURDFK (WKLVRUE 3DWFKp (WKLFRQ3URFHHG VXUJLFDO PHVKp (WKLFRQ&ROODJHQPHVK5HVRGRQW PHVKp 5HVRUED3DUDVRUEPHVKp5HVRUED'XDOPHVKp)D*RUH0\FURPHVKp)D*RUH3&/ VFDᚎROG$G9DQFHPDOHVOLQJp$066HUDWLPp6HUDJ:LHVVQHU Conclusions: 7)70RWLIPHVKUHSUHVHQWVWKHRQO\DOORSODVWLFPDWHULDOWKDWVKRZVLQ YLWURELRFRPSDWLELOLW\IRUWKHFHOOW\SHVFKDUDFWHULVLQJWKHSHOYLFᚐRRUDQGDEGRPLQDO ZDOO&HOODGKHVLRQWRWKHWHVWHGDOORSODVWLFPDWHULDOVUHJDUGLQJFHOOVRIWKHSHOYLF ᚐRRULVLQVXᚑFLHQW7KLVREVHUYDWLRQPD\H[SODLQWKHRFFXUUHQFHRIIRUHLJQERG\ UHDFWLRQDQGPLVVLQJLQWHJUDWLRQRIDOORSODVWLFPDWHULDOVLQWRWKHSHOYLFᚐRRU&RDWLQJ RIPHVKVZLWKDXWRORJRXVSODVPDUHVXOWVLQDQLPSURYHPHQWRIWKHELRFRPSDWLELOLW\ RIDOORSODVWLFPDWHULDOV
257
TO INVESTIGATE THE CHANGES OF CONCENTRATION OF TGF-β1 IN THE URINE OF PATIENTS WITH URETHRAL STRICTURES
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Results: 7KHUHVXOWVVKRZWKDWWKHFRQFHQWUDWLRQRI7*)˟LQXULQHZDVLQFUHDVHG VLJQLᚏFDQWO\ LQ H[SHULPHQWDO JURXS WKDQ LQ FRQWURO JURXS DIWHU WKH VXUJHU\ 0HDQZKLOH WKH FRQFHQWUDWLRQ RI 7*)˟ LQ XULQH LQ WKH SDWLHQWV RI H[SHULPHQWDO JURXS ZDV VLJQLᚏFDQWO\ GLᚎHUHQW EHIRUH DQG DIWHU WKH VXUJHU\᧤3 ᧥ ,Q WKH DQDO\VLVRIFRUUHODWLYHIDFWRUVWKHYDU\LQJUDQJHRIFRQFHQWUDWLRQRI7*)˟ZDV VLJQLᚏFDQWLQWKHXULQHRIDQWHULRUXUHWKUDOVWULFWXUHSDWLHQWVWKDQLQWKDWRISRVWHULRU RQHV᧤3 ᧥&RPSDUHG ZLWK HQG WR HQG DQDVWRPRVLV RU '9,8 SURFHGXUHV JUDIW VXEVWLWXWLRQ PHWKRG FDQ PDNH VLJQLᚏFDQW YDULDWLRQ RI JURZWK IDFWRU LQ XULQH᧤3 ᧥$JHKLVWRU\RIRSHUDWLRQDQGVXSUDSXELFF\VWRVWRP\GRQRWOHDG WRWKHVLJQLᚏFDQWYDULDWLRQRIFRQFHQWUDWLRQRI7*)˟LQWKHXULQHRISDWLHQWVDIWHU WKHVXUJHU\ Conclusions: 7KHUHVXOWVRIWKLVVWXG\LQGLFDWHGWKDWERWKWKHSRVLWLRQRIXUHWKUDO VWULFWXUHDQGWKHSDWWHUQRIVXUJHU\DUHUHODWHGWRWKHFRQFHQWUDWLRQRI7*)˟LQ WKHXULQHRISDWLHQWV KRZHYHUDJHRSHUDWLYHKLVWRU\DQGVXSUDSXELFF\VWRVWRP\ DUHQRWVLJQLᚏFDQWO\UHODWHGWRWKHFRQFHQWUDWLRQRI7*)˟LQWKHXULQHRISDWLHQWV
258
A NEWLY DEVELOPED COLLAGEN I-BASED MATRIX FOR THE TISSUE ENGINEERING OF UROTHELIAL IMPLANTS
)HLO*0DXUHU6-XVW/.UXJ-.RKOHU.6WHQ]O$6LHYHUW.' 1 Eberhard-Karls-University Tübingen, Dept. of Urology, Tübingen, Germany, 2 Eberhard Karls University, Institute of Anatomy, Tübingen, Germany, 3Eberhard Karls University, Centre For Regenerative Biology and Regenerative Medicine, Tübingen, Germany Introduction & Objectives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aterial & Methods: 8UHWHUWLVVXHVSHFLPHQVZHUHREWDLQHGIURPDGXOWSDWLHQWV XQGHUJRLQJRSHQWXPRXUVXUJHU\DFFRUGLQJWRWKHHWKLFVFRPPLWWHHDSSURYDO+8&V ZHUHLVRODWHGDQGODEHOOHGZLWKWKHᚐXRUHVFHQWFHOOOLQNHU3.+VHHGHGRQWRWKH FROODJHQPDWUL[DQGFXOWLYDWHGLQFRPSOHWHNHUDWLQRF\WHVHUXPIUHHPHGLXP&HOO DGKHUHQFHZDVLQGLUHFWO\DVFHUWDLQHGE\FRXQWLQJQRQDGKHUHQWFHOOVLQWKHFXOWXUH VXSHUQDWDQW *URZWK EHKDYLRXU ZDV VWXGLHG E\ SKDVH FRQWUDVW PLFURVFRS\ DQG FU\RVHFWLRQVRIWKHSRSXODWHGPDWUL[9LDELOLW\RI+8&VVHHGHGRQWRWKHFROODJHQ PDWUL[ZDVDQDO\]HGZLWKWKH:67DVVD\6WDELOLW\ZDVSURYHQE\PHFKDQLFDO PDQLSXODWLRQRIWKHFHOOPDWUL[LPSODQWV Results: +8&VJURZQRQWKHFROODJHQPDWUL[ZHUHDVKRPRJHQHRXVO\VSUHDGDV +8&V VHHGHG RQWR VWDQGDUG SODVWLF VXUIDFH $W GD\ DIWHU VHHGLQJ WKH IUDFWLRQ RI QRQDGKHUHQW +8&V ZDV VOLJKWO\ LQFUHDVHG FRPSDUHG WR WKH FRQWUROV ZKHUHDV DW GD\ ERWK JURXSV UHYHDOHG VLPLODU UDWHV DQG UHVSHFWLYHO\ 9LDELOLW\RI+8&VJURZLQJRQWKHPDWUL[UHYHDOHGRIWKHFRQWURO JURXSDWGD\7KHFHOOPDWUL[FRQVWUXFWVFRXOGEHHDVLO\GHWDFKHGIURPWKHFXOWXUH GLVK7KHLPSODQWVGHPRQVWUDWHGDKLJKPHFKDQLFDOVWDELOLW\DOORZLQJPDQLSXODWLRQ ZLWKVXUJLFDOLQVWUXPHQWV Conclusions: 7KHGDWDUHYHDOHGDJRRGLQYLWURELRFRPSDWLELOLW\DQGDSHUVLVWLQJ PHFKDQLFDO VWDELOLW\ RI WKH VWDQGDUGLVHG QHZ ERYLQH FROODJHQ ,EDVHG PDWUL[ :H FRQFOXGH WKDW WKH PDWUL[ LV ZHOO VXLWDEOH IRU FRQVWUXFWLRQ RI XURWKHOLDO FHOO PDWUL[ LPSODQWV IRU UHFRQVWUXFWLYH VXUJHU\ RI XUHWKUD DQG XUHWHU ,Q RQJRLQJ H[SHULPHQWV PXOWLOD\HUHG XURWKHOLXP ZLOO EH JURZQ RQ WKH PDWUL[ DQG DQDO\VHG LPPXQRKLVWRFKHPLFDOO\IRUGLᚎHUHQWLDWLRQ
259
RECTOSIGMOID VAGINOPLASTY AND ITS IMPACT ON PSYCHOSOCIAL AND SEXUAL LIFE IN PATIENTS WITH VAGINAL AGENESIS
'MRUGMHYLF0//DEXV/%L]LF06WDQRMHYLF' University of Belgrade, Medical School, Dept. of Urology, Belgrade, Serbia Introduction & Objectives: 7KH PDLQ JRDO LQ SDWLHQWV ZLWK 0D\HU5RNLWDQVN\ .¾VWHU+DXVHU V\QGURPH YDJLQDO DJHQHVLV LV FUHDWLRQ RI D QHRYDJLQD WKDW ZLOO VDWLVI\ SDWLHQWಬV GHVLUH :H HYDOXDWHG VH[XDO DQG SV\FKRVRFLDO DGMXVWPHQW LQ SDWLHQWVZKRXQGHUZHQWUHFWRVLJPRLGYDJLQRSODVW\GXHWRYDJLQDODJHQHVLV Material & Methods: :HHYDOXDWHGZRPHQDJHGWR\HDUV PHDQ ZKR XQGHUZHQW UHFWRVLJPRLG YDJLQRSODVW\ 6H[XDO DQG SV\FKRVRFLDO DSSUDLVDO LQFOXGHGWKH)HPDOH6H[XDO)XQFWLRQ,QGH[ )6), %HFNಬV'HSUHVVLRQ,QYHQWRU\ %', DQGVWDQGDUGL]HGTXHVWLRQQDLUHVDERXWSRVWRSHUDWLYHVDWLVIDFWLRQVRFLDODQG VH[XDODGMXVWPHQW Results: 7KH PHDQ IROORZXS ZDV \HDUV UDQJH PRQWKV WR \HDUV 0HDQ)6),QGH[ZDV UDQJH ZLWKFXWRᚎVFRUHIRUVH[XDO G\VIXQFWLRQ2XWRIWKHZRPHQ KDGVH[XDOG\VIXQFWLRQ0HDQ%HFN 'HSUHVVLRQ,QYHQWRU\ %', ZDV FXWRᚎVFRUH IRUQRQGHSUHVVLRQ $ ZRPHQ ZDVZLWKRXWV\PSWRPVRIGHSUHVVLRQ %',UDQJHGIURP KDGPRGHUDWHDQGRQHKDGVHYHUHGHSUHVVLRQ %', 7KHUHZHUHVLJQLᚏFDQWOHYHO S ZLWKLQKLJKVDWLVIDFWLRQVFRUHLQ)6),DQGORZ%',UHVXOWVWKDWLQGLFDWHV
Eur Urol Suppl 2010;9(2):109
256
IN VITRO TEST SYSTEM FOR THE EVALUATION OF THE BIOCOMPATIBILITY OF ALLOPLASTIC MATERIALS
(LPHU&*HUXOOLV+%DJQHU-/DPPHUV%-7KHXHUNDXI,5DPRQ$ 2WWR7&HQWHUIRU,QQRYDWLYH7HFKQLTXHVLQ0HGLFLQH 1 Lukas Hospital, Dept. of Urology, Neuss, Germany, 2Lukas Hospital, Dept. of Surgery, Neuss, Germany, 3Lukas Hospital, Dept. of Pathology, Neuss, Germany, 4 International Tissue Engineering Research Association, Itera, Bad Aachen, Germany Introduction & Objectives: $OORSODVWLFPDWHULDOVDUHZLGHO\DSSOLHGLQWKHVXUJLFDO WKHUDSLHVIRUKHUQLDLQFRQWLQHQFHDQGSURODSVHVLWXDWLRQV7KH$PHULFDQ)RRGDQG 'UXJ$GPLQLVWUDWLRQ )'$ LVVXHGDZDUQLQJFRQFHUQLQJWKHDSSOLFDWLRQRIDOORSODVWLF PDWHULDOVIRUWKHVXUJLFDOWUHDWPHQWRIIHPDOHLQFRQWLQHQFHDQGSURODSVHUHSRUWLQJ PRUHWKDQVHYHUHDGYHUVHHYHQWVDVVRFLDWHGZLWKWKHXVHGPDWHULDOV6SHFLDO VXUJLFDOWUDLQLQJWRJHWKHUZLWKWKHPDQDJHPHQWRIULVNDQG$(vVDUHUHFRPPHQGHG ZZZIGDJRYFGUKVDIHW\VXUJLFDOPHVKKWPO 7KH LPSURYHPHQW RI WKH ELRFRPSDWLELOLW\FRDWLQJIRUHLJQPDWHULDOVZLWKHJDXWRORJRXVVXEVWDQFHVVHHPV WREHPDQGDWRU\ Material & Methods: ,QRUGHUWRLQYHVWLJDWHLIFRDWLQJRIDOORSODVWLFPDWHULDOVZLWK DXWRORJRXVPDWHULDOFDQOHDGWRLPSURYHGELRFRPSDWLELOLW\DQGLIDOORSODVWLFPDWHULDOV DUHLQWHJUDWHGLQWRWKHVWUXFWXUHRIWKHSHOYLFᚐRRUWKHIROORZLQJVWXG\DSSURDFKZDV HVWDEOLVKHG SDWLHQWVZHUHLQFOXGHG0\REODVWVHQGRWKHOLDOFHOOVDQGᚏEUREODVWV RIHDFKSDWLHQWZHUHFXOWLYDWHGVHSDUDWHO\&HOOFRXQWDQGJURZWKDUHFRPSDUDEOH LQ DOO DSSURDFKHV ZLWKLQ D WHVW GXUDWLRQ RI ZHHNV ,PPXQRKLVWRFKHPLFDO DQG PLFURVFRSLF HYDOXDWLRQ RI FHOO FRXQW DQG DGKHUHQFH LV SHUIRUPHG FRQVLGHULQJ WKH FKRVHQ FRDWLQJ IRU WKH DOORSODVWLF PDWHULDO $GGLWLRQDOO\ ZH SHUIRUPHG LPPXQRF\WRFKHPLVWU\ LQ RUGHU WR YHULI\ WKH SUHVHQFH RI WKHVH FHOOV SURYLQJ WKH H[SUHVVLRQ RI VSHFLᚏF FHOO PDUNHUV 7KH GHWHFWLRQ RI P\REODVWV ZDV UHDOLVHG E\ ˞VDUFRPHULFDFWLQDQGGHVPLQDVPDUNHUVRIP\RJHQHGLᚎHUHQWLDWLRQ)LEUREODVWV ZHUHGHPRQVWUDWHGE\WKHXVHRIDQWLERGLHVWDUJHWLQJYLPHQWLQZKHUHDVDQWLERGLHV DJDLQVW&'ZHUHXWLOLVHGIRUWKHGHWHFWLRQRIHQGRWKHOLDOFHOOV$GKHUHQFHDQG FHOO FRXQW DUH DVVHVVHG PLFURVFRSLFDOO\ DQG E\ LPPXQRKLVWRFKHPLVWU\ DIWHU FR LQFXEDWLRQRIWKHFHOOVZLWKGLᚎHUHQWW\SHVRIDOORSODVWLFPHVKV Results: GLᚎHUHQWFRPPRQO\XVHGDOORSODVWLFPDWHULDOVZHUHLQYHVWLJDWHG&HOO FRXQW DQG JURZWK ZHUH FRPSDUDEOH LQ DOO DSSURDFKHV ZLWKLQ D WHVWGXUDWLRQ RI ZHHNV2QO\RQ7)70RWLIPHVKFHOOVDUHVKRZLQJDGKHUHQFHDQGJURZWK2QDOO RWKHU PHVKV WKHUH LV QR HYLGHQFH RI FHOO DGKHUHQFH WR WKH UHVSHFWLYH DOORSODVWLF PDWHULDOV7KHLQYLWURDQDO\VHVZHUHLQGHSHQGHQWIURPJHQGHUDQGLQGLYLGXDOSDWLHQW IHDWXUHV 5HJDUGLQJ WLVVXH VSHFLI\ GLᚎHUHQW FHOO JURZWK FDQ GH GHᚏQHG LQ IDYRXU RIᚏEUREODVWV ᚏEUREODVWV!P\REODVWV!HQGRWKHOLDOFHOOV,PSURYHGFHOODGKHVLRQ ZDVIRXQGRQDOORSODVWLFPHVKVFRYHUHGZLWKDXWRORJRXVSODVPDFRPSDUHGWRQRQ FRYHUHG PHVKV 7KLV ᚏQGLQJ LQFOXGHV DOO W\SHV RI FHOOV EHLQJ WHVWHG $ FRYHULQJ RI PHVKV ZLWK VHUXP RU FROODJHQ VHHPV WR EH RI GLVDGYDQWDJH /RRNLQJ DW WKH LQWHQVLW\ RI FHOO DGKHVLRQ RQ WKH GLᚎHUHQW PHVKV WKH IROORZLQJ UDQNLQJ FDQ EH GLVSRVHG 7)70RWLIPHVKp3UR[\%LRPHGLFDO'\QDPHVKp)(*7H[WLOWHFKQLN 6XUJLPHVKp $VSLGH 0HGLFDO 5HVRUED 3'6 3ODWHp -RKQVRQ -RKQVRQ 0HUVLOHQH%DQG-RKQVRQ -RKQVRQ9LWDPHVKp3UR[\%LRPHGLFDO9LFU\O%DQG -RKQVRQ -RKQVRQ 7L/RRSp 3)0&HOO DGKHVLRQ RQ WKH IROORZLQJ DOORSODVWLF PDWHULDOVLVQRWVHHQQHLWKHULQWKHXQFRYHUHGQRULQWKHFRYHUHGDSSURDFK (WKLVRUE 3DWFKp (WKLFRQ3URFHHG VXUJLFDO PHVKp (WKLFRQ&ROODJHQPHVK5HVRGRQW PHVKp 5HVRUED3DUDVRUEPHVKp5HVRUED'XDOPHVKp)D*RUH0\FURPHVKp)D*RUH3&/ VFDᚎROG$G9DQFHPDOHVOLQJp$066HUDWLPp6HUDJ:LHVVQHU Conclusions: 7)70RWLIPHVKUHSUHVHQWVWKHRQO\DOORSODVWLFPDWHULDOWKDWVKRZVLQ YLWURELRFRPSDWLELOLW\IRUWKHFHOOW\SHVFKDUDFWHULVLQJWKHSHOYLFᚐRRUDQGDEGRPLQDO ZDOO&HOODGKHVLRQWRWKHWHVWHGDOORSODVWLFPDWHULDOVUHJDUGLQJFHOOVRIWKHSHOYLF ᚐRRULVLQVXᚑFLHQW7KLVREVHUYDWLRQPD\H[SODLQWKHRFFXUUHQFHRIIRUHLJQERG\ UHDFWLRQDQGPLVVLQJLQWHJUDWLRQRIDOORSODVWLFPDWHULDOVLQWRWKHSHOYLFᚐRRU&RDWLQJ RIPHVKVZLWKDXWRORJRXVSODVPDUHVXOWVLQDQLPSURYHPHQWRIWKHELRFRPSDWLELOLW\ RIDOORSODVWLFPDWHULDOV
257
TO INVESTIGATE THE CHANGES OF CONCENTRATION OF TGF-β1 IN THE URINE OF PATIENTS WITH URETHRAL STRICTURES
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Results: 7KHUHVXOWVVKRZWKDWWKHFRQFHQWUDWLRQRI7*)˟LQXULQHZDVLQFUHDVHG VLJQLᚏFDQWO\ LQ H[SHULPHQWDO JURXS WKDQ LQ FRQWURO JURXS DIWHU WKH VXUJHU\ 0HDQZKLOH WKH FRQFHQWUDWLRQ RI 7*)˟ LQ XULQH LQ WKH SDWLHQWV RI H[SHULPHQWDO JURXS ZDV VLJQLᚏFDQWO\ GLᚎHUHQW EHIRUH DQG DIWHU WKH VXUJHU\᧤3 ᧥ ,Q WKH DQDO\VLVRIFRUUHODWLYHIDFWRUVWKHYDU\LQJUDQJHRIFRQFHQWUDWLRQRI7*)˟ZDV VLJQLᚏFDQWLQWKHXULQHRIDQWHULRUXUHWKUDOVWULFWXUHSDWLHQWVWKDQLQWKDWRISRVWHULRU RQHV᧤3 ᧥&RPSDUHG ZLWK HQG WR HQG DQDVWRPRVLV RU '9,8 SURFHGXUHV JUDIW VXEVWLWXWLRQ PHWKRG FDQ PDNH VLJQLᚏFDQW YDULDWLRQ RI JURZWK IDFWRU LQ XULQH᧤3 ᧥$JHKLVWRU\RIRSHUDWLRQDQGVXSUDSXELFF\VWRVWRP\GRQRWOHDG WRWKHVLJQLᚏFDQWYDULDWLRQRIFRQFHQWUDWLRQRI7*)˟LQWKHXULQHRISDWLHQWVDIWHU WKHVXUJHU\ Conclusions: 7KHUHVXOWVRIWKLVVWXG\LQGLFDWHGWKDWERWKWKHSRVLWLRQRIXUHWKUDO VWULFWXUHDQGWKHSDWWHUQRIVXUJHU\DUHUHODWHGWRWKHFRQFHQWUDWLRQRI7*)˟LQ WKHXULQHRISDWLHQWV KRZHYHUDJHRSHUDWLYHKLVWRU\DQGVXSUDSXELFF\VWRVWRP\ DUHQRWVLJQLᚏFDQWO\UHODWHGWRWKHFRQFHQWUDWLRQRI7*)˟LQWKHXULQHRISDWLHQWV
258
A NEWLY DEVELOPED COLLAGEN I-BASED MATRIX FOR THE TISSUE ENGINEERING OF UROTHELIAL IMPLANTS
)HLO*0DXUHU6-XVW/.UXJ-.RKOHU.6WHQ]O$6LHYHUW.' 1 Eberhard-Karls-University Tübingen, Dept. of Urology, Tübingen, Germany, 2 Eberhard Karls University, Institute of Anatomy, Tübingen, Germany, 3Eberhard Karls University, Centre For Regenerative Biology and Regenerative Medicine, Tübingen, Germany Introduction & Objectives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aterial & Methods: 8UHWHUWLVVXHVSHFLPHQVZHUHREWDLQHGIURPDGXOWSDWLHQWV XQGHUJRLQJRSHQWXPRXUVXUJHU\DFFRUGLQJWRWKHHWKLFVFRPPLWWHHDSSURYDO+8&V ZHUHLVRODWHGDQGODEHOOHGZLWKWKHᚐXRUHVFHQWFHOOOLQNHU3.+VHHGHGRQWRWKH FROODJHQPDWUL[DQGFXOWLYDWHGLQFRPSOHWHNHUDWLQRF\WHVHUXPIUHHPHGLXP&HOO DGKHUHQFHZDVLQGLUHFWO\DVFHUWDLQHGE\FRXQWLQJQRQDGKHUHQWFHOOVLQWKHFXOWXUH VXSHUQDWDQW *URZWK EHKDYLRXU ZDV VWXGLHG E\ SKDVH FRQWUDVW PLFURVFRS\ DQG FU\RVHFWLRQVRIWKHSRSXODWHGPDWUL[9LDELOLW\RI+8&VVHHGHGRQWRWKHFROODJHQ PDWUL[ZDVDQDO\]HGZLWKWKH:67DVVD\6WDELOLW\ZDVSURYHQE\PHFKDQLFDO PDQLSXODWLRQRIWKHFHOOPDWUL[LPSODQWV Results: +8&VJURZQRQWKHFROODJHQPDWUL[ZHUHDVKRPRJHQHRXVO\VSUHDGDV +8&V VHHGHG RQWR VWDQGDUG SODVWLF VXUIDFH $W GD\ DIWHU VHHGLQJ WKH IUDFWLRQ RI QRQDGKHUHQW +8&V ZDV VOLJKWO\ LQFUHDVHG FRPSDUHG WR WKH FRQWUROV ZKHUHDV DW GD\ ERWK JURXSV UHYHDOHG VLPLODU UDWHV DQG UHVSHFWLYHO\ 9LDELOLW\RI+8&VJURZLQJRQWKHPDWUL[UHYHDOHGRIWKHFRQWURO JURXSDWGD\7KHFHOOPDWUL[FRQVWUXFWVFRXOGEHHDVLO\GHWDFKHGIURPWKHFXOWXUH GLVK7KHLPSODQWVGHPRQVWUDWHGDKLJKPHFKDQLFDOVWDELOLW\DOORZLQJPDQLSXODWLRQ ZLWKVXUJLFDOLQVWUXPHQWV Conclusions: 7KHGDWDUHYHDOHGDJRRGLQYLWURELRFRPSDWLELOLW\DQGDSHUVLVWLQJ PHFKDQLFDO VWDELOLW\ RI WKH VWDQGDUGLVHG QHZ ERYLQH FROODJHQ ,EDVHG PDWUL[ :H FRQFOXGH WKDW WKH PDWUL[ LV ZHOO VXLWDEOH IRU FRQVWUXFWLRQ RI XURWKHOLDO FHOO PDWUL[ LPSODQWV IRU UHFRQVWUXFWLYH VXUJHU\ RI XUHWKUD DQG XUHWHU ,Q RQJRLQJ H[SHULPHQWV PXOWLOD\HUHG XURWKHOLXP ZLOO EH JURZQ RQ WKH PDWUL[ DQG DQDO\VHG LPPXQRKLVWRFKHPLFDOO\IRUGLᚎHUHQWLDWLRQ
259
RECTOSIGMOID VAGINOPLASTY AND ITS IMPACT ON PSYCHOSOCIAL AND SEXUAL LIFE IN PATIENTS WITH VAGINAL AGENESIS
'MRUGMHYLF0//DEXV/%L]LF06WDQRMHYLF' University of Belgrade, Medical School, Dept. of Urology, Belgrade, Serbia Introduction & Objectives: 7KH PDLQ JRDO LQ SDWLHQWV ZLWK 0D\HU5RNLWDQVN\ .¾VWHU+DXVHU V\QGURPH YDJLQDO DJHQHVLV LV FUHDWLRQ RI D QHRYDJLQD WKDW ZLOO VDWLVI\ SDWLHQWಬV GHVLUH :H HYDOXDWHG VH[XDO DQG SV\FKRVRFLDO DGMXVWPHQW LQ SDWLHQWVZKRXQGHUZHQWUHFWRVLJPRLGYDJLQRSODVW\GXHWRYDJLQDODJHQHVLV Material & Methods: :HHYDOXDWHGZRPHQDJHGWR\HDUV PHDQ ZKR XQGHUZHQW UHFWRVLJPRLG YDJLQRSODVW\ 6H[XDO DQG SV\FKRVRFLDO DSSUDLVDO LQFOXGHGWKH)HPDOH6H[XDO)XQFWLRQ,QGH[ )6), %HFNಬV'HSUHVVLRQ,QYHQWRU\ %', DQGVWDQGDUGL]HGTXHVWLRQQDLUHVDERXWSRVWRSHUDWLYHVDWLVIDFWLRQVRFLDODQG VH[XDODGMXVWPHQW Results: 7KH PHDQ IROORZXS ZDV \HDUV UDQJH PRQWKV WR \HDUV 0HDQ)6),QGH[ZDV UDQJH ZLWKFXWRᚎVFRUHIRUVH[XDO G\VIXQFWLRQ2XWRIWKHZRPHQ KDGVH[XDOG\VIXQFWLRQ0HDQ%HFN 'HSUHVVLRQ,QYHQWRU\ %', ZDV FXWRᚎVFRUH IRUQRQGHSUHVVLRQ $ ZRPHQ ZDVZLWKRXWV\PSWRPVRIGHSUHVVLRQ %',UDQJHGIURP KDGPRGHUDWHDQGRQHKDGVHYHUHGHSUHVVLRQ %', 7KHUHZHUHVLJQLᚏFDQWOHYHO S ZLWKLQKLJKVDWLVIDFWLRQVFRUHLQ)6),DQGORZ%',UHVXOWVWKDWLQGLFDWHV
Eur Urol Suppl 2010;9(2):109