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263 Central-and periph in vitro quantitative autoradiographic studies of the eral-type benzodiazepine receptors in fyn-deficient mice
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CENTRAL- AND PERIPH IN VITRO QUANTITATIVE AUTORADIOGRAPHIC STUDIES OF THE ERAL-TYPE BENZODIAZEPINE RECEPTORS IN FYN-DEFICIENT MICE. Dept of Neurophysiol., Tokyo Inst. of Psychiat., Tokyo 156l, Dept of Psychol., Fat. of Lett., Univ. of Tokyo, Tokyo 1132, Dept of Neurobiol. Behav. Genet., Nat1 Inst. of Physiol. Sci., Okazaki 4443 YUIUE NAKAMOTO’ , TSUYOSHI MIYAKAWA2, TAKESHI YAG13, HIROAKI NIK12, MITSUNOBU YOSHII’ Fyn tyrosin’e kinase deficient mice progress audiogenic seizure susceptibility and fearfulness (Miyakawa et al., 1995; 1996). The density of central- type benzodiazepine receptors (CBR) decreases in the brain of Fyn-deficient mice whereas that of peripheral-type benzodiazepine receptors (PBR) shows no significant difference between Fyn-deficient and control mice (Nakamoto et al., 1996). In the present study, we have further investigated distributions of the CBR and the PBR by in vitro quantitative autoradiography using [3H]flunitrazepam and [3H]PK11195 as ligands for CBR and PBR, respectively.In Fyn-deficient mice, [3H]flunitrazepam binding was decreased widely, particularly in front-parietal cortex, hippocampus, globus pallidus and amygdala, whereas rH]PK11195 binding showed no significant alterations. The results suggests that seizure susceptibility and fearfulness in Fyn-deficient mice may result from dysfunction of the GABAergic system in these brain regions.
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CENTRAL- AND PERIPH IN VITRO QUANTITATIVE AUTORADIOGRAPHIC STUDIES OF THE ERAL-TYPE BENZODIAZEPINE RECEPTORS IN FYN-DEFICIENT MICE. Dept of Neurophysiol., Tokyo Inst. of Psychiat., Tokyo 156l, Dept of Psychol., Fat. of Lett., Univ. of Tokyo, Tokyo 1132, Dept of Neurobiol. Behav. Genet., Nat1 Inst. of Physiol. Sci., Okazaki 4443 YUIUE NAKAMOTO’ , TSUYOSHI MIYAKAWA2, TAKESHI YAG13, HIROAKI NIK12, MITSUNOBU YOSHII’ Fyn tyrosin’e kinase deficient mice progress audiogenic seizure susceptibility and fearfulness (Miyakawa et al., 1995; 1996). The density of central- type benzodiazepine receptors (CBR) decreases in the brain of Fyn-deficient mice whereas that of peripheral-type benzodiazepine receptors (PBR) shows no significant difference between Fyn-deficient and control mice (Nakamoto et al., 1996). In the present study, we have further investigated distributions of the CBR and the PBR by in vitro quantitative autoradiography using [3H]flunitrazepam and [3H]PK11195 as ligands for CBR and PBR, respectively.In Fyn-deficient mice, [3H]flunitrazepam binding was decreased widely, particularly in front-parietal cortex, hippocampus, globus pallidus and amygdala, whereas rH]PK11195 binding showed no significant alterations. The results suggests that seizure susceptibility and fearfulness in Fyn-deficient mice may result from dysfunction of the GABAergic system in these brain regions.