264 DETRUSOR CONTRACTILE RESPONSE RESISTANT TO CHOLINERGIC AND PURINERGIC PATHWAY BLOCKADE

264 DETRUSOR CONTRACTILE RESPONSE RESISTANT TO CHOLINERGIC AND PURINERGIC PATHWAY BLOCKADE

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BETA-NAD+ AND CYCLIC ADP-RIBOSE INDUCE RELAXATION OF THE HUMAN DETRUSOR SMOOTH MUSCLE

%RXFKHORXFKH3%RXFKHORXFKH.1RUGOLQJ- Koege Hospital, University of Copenhagen, Smooth Muscle Research Center, Dept. of Clinical Biochemistry, Koege, Denmark, 2Herlev Hospital, University of Copenhagen, Dept. of Urology, Herlev, Denmark 1

Introduction & Objectives: ˟1$'DQGLWVPHWDEROLWHF\FOLF$'3ULERVH F$'35  DUH QRYHO QXFOHRWLGHV UHOHDVHG WRJHWKHU ZLWK $73 IURP SRVWJDQJOLRQLF QHUYH WHUPLQDOV LQ WKH KXPDQ GHWUXVRU PXVFOH 6FLHQFHVLJQDOLQJ  YRO      :H LQYHVWLJDWHG WKH HᚎHFWV RI H[RJHQRXV DGPLQLVWHUHG ˟1$' DQG F$'35 RQ VSRQWDQHRXV GHYHORSHG DQG $73 LQGXFHG FRQWUDFWLRQV LQ KXPDQ GHWUXVRU VPRRWKPXVFOHVWULSV Material & Methods: +XPDQGHWUXVRUELRSVLHVZHUHREWDLQHGIURPPDFURVFRSLF QRUPDODUHDVRIVLGHZDOOVRIWKHXULQDU\EODGGHUIURPPDOHSDWLHQWVXQGHUJRLQJ F\VWHFWRP\ GXH WR EODGGHU FDQFHU /RQJLWXGLQDO GHWUXVRU VWULSV  PJ  ZHUH PRXQWHG LQ D P\RJUDSK DQG WKH GHYHORSHG WHQVLRQ ZDV UHFRUGHG GXULQJ H[SHULPHQWV 6PDOO FRQGXFWDQFH &DDFWLYDWHG . 6.  FKDQQHOV H[SUHVVLRQ LQ KXPDQGHWUXVRUPXVFOHZDVGHWHUPLQHGXVLQJUHDOWLPHTXDQWLWDWLYH3&5 Results: ˟1$' w0P0 DQGF$'35 w0 LQGXFHGDFRQFHQWUDWLRQ GHSHQGHQW UHOD[DWLRQ RI GHWUXVRU PXVFOH UHVWLQJ WRQH LQ   RI WKH GHWUXVRU SUHSDUDWLRQV Q  6SRQWDQHRXVO\GHYHORSHGFRQWUDFWLRQV !RIWKHELRSVLHV  ZHUHFRPSOHWHO\LQKLELWHGE\˟1$' P0 DQGF$'35 w0 2QH[SRVXUH WR $73  P0  GHWUXVRU VWULSV H[KLELWHG D GRVHGHSHQGHQW ELSKDVLF IRUFH UHVSRQVH FKDUDFWHUL]HG E\ D UDSLG DQG WUDQVLHQW FRQWUDFWLRQ IROORZHG E\ D VORZ

MIRABEGRON (YM178), A NOVEL β3-ADRENOCEPTOR (AR) AGONIST, INCREASED FUNCTIONAL BLADDER CAPACITY AND DECREASED MICTURITION FREQUENCY IN CONSCIOUS WATER-LOADED CYNOMOLGUS MONKEYS

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OBSTRUCTION ENHANCES RHO-KINASE PATHWAY AND DIMINISHES PROTEIN KINASE C PATHWAY IN CARBACHOL-INDUCED CALCIUM SENSITIZATION IN ALPHA-TOXIN PERMEABILIZED GUINEA-PIG DETRUSOR SMOOTH MUSCLE

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Eur Urol Suppl 2010;9(2):111