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276 CHANGES IN BLADDER WALL PERFUSION IN THE OVERACTIVE OBSTRUCTED BLADDER
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.DUEDODHL0(NPDQ08YHOLXV%6Z¦UG. 1 Lund University, Dept. of Experimental Medical Science, Lund, Sweden, 2Lund University, Dept. of Urology, Clinical Sciences, Lund, Sweden Introduction & Objectives: &DYHRODHDUHRPHJDVKDSHGPHPEUDQHRUJDQHOOHV WKDWKDYHEHHQVKRZQWRVHTXHVWHUDQGUHJXODWHLPSRUWDQWVLJQDOOLQJLQWHUPHGLDULHV &DYHRODELRJHQHVLVGHSHQGVRQSURWHLQVIURPWKHFDYHROLQDQGFDYLQIDPLOLHVDQG RQ PHPEUDQH FKROHVWHURO &DYHROLQGHᚏFLHQF\ LV DVVRFLDWHG ZLWK VXEVWDQWLDO XURJHQLWDODOWHUDWLRQVLQPDOHPLFH7KHVHFKDQJHVLQFOXGHSURVWDWHK\SHUWURSK\ GLVWHQVLRQ RI WKH VHPLQDO YHVLFOHV GLVWXUEHG HUHFWLOH IXQFWLRQ DQG LPSDLUHG PXVFDULQLFFRQWUDFWLOLW\RILVRODWHGGHWUXVRUVWULSVLQYLWUR:KHWKHUWKHFRQWUDFWLOH FKDQJHV LQ WKH EODGGHU DUH VHFRQGDU\ WR RXWᚐRZ REVWUXFWLRQ FDXVHG E\ WKH K\SHUWURSKLFSURVWDWHRUUHᚐHFWDSULPDU\UROHRIFDYHRODHLQGHWUXVRUVLJQDOOLQJLV QRWNQRZQ7KLVZDVDGGUHVVHGE\PDNLQJDGHWDLOHGIXQFWLRQDOFKDUDFWHUL]DWLRQRI GHWUXVRUFRQWUDFWLOLW\LQZLOGW\SH :7 DQGFDYHROLQGHᚏFLHQW .2 IHPDOHPLFH Material & Methods: 'HWUXVRUVIURPZHHNROG.2DQGFRQJHQLF:7IHPDOH PLFHZHUHH[DPLQHGLQYLWURXVLQJP\RJUDSK\'LXUHVLVZDVHVWLPDWHGE\ZDWHU FRQVXPSWLRQ /HQJWKWHQVLRQ UHODWLRQVKLSV ZHUH JHQHUDWHG DQG FRQWUDFWLOLW\ LQ UHVSRQVH WR WKH PXVFDULQLF DJRQLVW FDUEDFKRO ZDV GHWHUPLQHG DW WKH RSWLPXP OHQJWK / IRU DFWLYH IRUFH :HVWHUQ EORWWLQJ ZDV XVHG WR DVVHVV FRQWHQWV RI FDYHROLQFDYLQDQGPXVFDULQLF0UHFHSWRUV Results: .2PLFHFRQVXPHGVLJQLᚏFDQWO\OHVVZDWHUSHUGD\VXJJHVWLQJUHGXFHG GLXUHVLV7KHEODGGHUWRERG\ZHLJKWUDWLRZDVQRWFKDQJHGLQ.2PLFH7KHSDVVLYH OHQJWKWHQVLRQUHODWLRQVKLSRIWKH.2EODGGHUVZDVOHIWVKLIWHGLQFRPSDULVRQZLWK :7EODGGHUV0D[LPDOFDUEDFKROLQGXFHGIRUFHDW/ZDVLQFUHDVHGLQ.2EODGGHU ZKHQQRUPDOL]HGWRKLJK.FRQWUDFWLRQ Conclusions: *HQHWLFDEODWLRQRIFDYHRODHLQWKHIHPDOHPRXVHOHDGVWRVXEVWDQWLDO IXQFWLRQDOFKDQJHVLQWKHEODGGHUUXOLQJRXWREVWUXFWLRQUHODWHGK\SHUWURSK\DVD FRQWULEXWLQJ IDFWRU 7KH OHIWVKLIW RI WKH SDVVLYH OHQJWKWHQVLRQ UHODWLRQVKLS LQ WKH .2EODGGHUPD\UHᚐHFWDGDSWDWLRQWRUHGXFHGGLXUHVLVRULQFUHDVHGWLVVXHVWLᚎQHVV &DUEDFKRO FRQWUDFWLRQ LV LQFUHDVHG LQ WKH FDYHRODHGHᚏFLHQW IHPDOH GHWUXVRU VXJJHVWLQJWKDWWKHSUHVHQFHRIFDYHRODHVXSSUHVVHVPXVFDULQLFVLJQDOOLQJLQWKH IHPDOHPRXVHEODGGHU
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6FKHHSH-5 $PHOLQN$ :ROᚎHQEXWWHO.3 'H-RQJ%:' .RN'- 1 Erasmus Medical Center, Dept. of Urology, Paediatric Urology, Rotterdam, The Netherlands, 2Erasmus Medical Center, Dept. of Radiotherapy, Rotterdam, The Netherlands, 3Erasmus Medical Center, Dept. of Pathology, Rotterdam, The Netherlands Introduction & Objectives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aterial & Methods: 2[\JHQDWLRQ DQG EORRG YROXPH RI WKH EODGGHU ZDOO ZDV PHDVXUHG LQ YLYR E\ GLᚎHUHQWLDO SDWKOHQJWK VSHFWURVFRS\ '36 XVLQJ JODVV ᚏEHUV LQ JXLQHD SLJV VKDP RSHUDWHG Q DQG XUHWKUDOO\ REVWUXFWHG Q 0HDVXUHPHQWVZHUHSHUIRUPHGPDLQO\RQWKHEODGGHUWRSGXULQJPXOWLSOHYRLGLQJ ᚏOOLQJF\FOHV EHIRUHVXUJHU\ZHHNVDIWHU 6+$0DQGREVWUXFWHG DQGHLJKW ZHHNVDIWHU 8URG\QDPLFLQYHVWLJDWLRQVGXULQJWKHZHHNSHULRGSURYLGHG GDWD RQ EODGGHUSUHVVXUH FRPSOLDQFH FRQWUDFWLOLW\ DQG ದRYHUDFWLYLW\ DQG XULQH ᚐRZUDWH Results: %HIRUHVXUJHU\LQERWKJURXSVDQGZHHNVDIWHU6+$0VXUJHU\R[\JHQ VDWXUDWLRQ LQ WKH EODGGHU ZDOO ZDV DERYH GXULQJ ᚏOOLQJ ,W GURSSHG GXULQJ
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ROLE OF STROMAL FIBROBLASTS INTERACTION WITH PROSTATIC CANCER CELLS IN ACHIEVEMENT OF METASTATIC PHENOTYPE
6HUQL6*LDQQRQL(0DVLHUL/&DORULQL//DQFLRWWL0,HUDUGL$ 0LQHUYLQL$/DSLQL$&DULQL0&KLDUXJL3 1 University of Florence, Dept. of Urology, Florence, Italy, 2University of Florence, Dept. of Biochemistry, Florence, Italy Introduction & Objectives: 7XPRUDO PLFURHQYLURQPHQW DOVR FDOOHG ಯUHDFWLYH VWURPDರ SOD\V DQ LPSRUWDQW UROH LQ SURVWDWH FDQFHU SURJUHVVLRQ DQG LQ WKH DFKLHYHPHQWRIDPHWDVWDWLFSKHQRW\SH3DUWLFXODUO\DFWLYDWHGᚏEUREODVWV $) ZLWK DQH[QRYRH[SUHVVLRQRI˞60$SURWHLQ VPRRWKPXVFOHDFWLQ DUHVXSSRVHGWR HQKDQFH WXPRUDO FHOO DJJUHVVLYH FKDUDFWHUV DQG PHWDVWDWLF FDSDFLW\ $LP RI RXU VWXG\ZDVWRDQDO\]HWKHELXQLYRFDOLQWHUDFWLRQEHWZHHQᚏEUREODVWVDQGSURVWDWLF FDQFHU FHOOV DQG WR HVWDEOLVK KRZ WXPRUDO FHOOV DFKLHYH WKH DJJUHVVLYH DQG PHWDVWDWLFSKHQRW\SH Material & Methods: :HSHUIRUPHGFXOWXUHVRIᚏEUREODVWVXQWUDQVIRUPHGSURVWDWLF HSLWKHOLDO FHOOV 317 ZHUH FXOWLYDWHG LQ 530, PHGLXP DQG SURVWDWLF FDQFHU
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.DUEDODHL0(NPDQ08YHOLXV%6Z¦UG. 1 Lund University, Dept. of Experimental Medical Science, Lund, Sweden, 2Lund University, Dept. of Urology, Clinical Sciences, Lund, Sweden Introduction & Objectives: &DYHRODHDUHRPHJDVKDSHGPHPEUDQHRUJDQHOOHV WKDWKDYHEHHQVKRZQWRVHTXHVWHUDQGUHJXODWHLPSRUWDQWVLJQDOOLQJLQWHUPHGLDULHV &DYHRODELRJHQHVLVGHSHQGVRQSURWHLQVIURPWKHFDYHROLQDQGFDYLQIDPLOLHVDQG RQ PHPEUDQH FKROHVWHURO &DYHROLQGHᚏFLHQF\ LV DVVRFLDWHG ZLWK VXEVWDQWLDO XURJHQLWDODOWHUDWLRQVLQPDOHPLFH7KHVHFKDQJHVLQFOXGHSURVWDWHK\SHUWURSK\ GLVWHQVLRQ RI WKH VHPLQDO YHVLFOHV GLVWXUEHG HUHFWLOH IXQFWLRQ DQG LPSDLUHG PXVFDULQLFFRQWUDFWLOLW\RILVRODWHGGHWUXVRUVWULSVLQYLWUR:KHWKHUWKHFRQWUDFWLOH FKDQJHV LQ WKH EODGGHU DUH VHFRQGDU\ WR RXWᚐRZ REVWUXFWLRQ FDXVHG E\ WKH K\SHUWURSKLFSURVWDWHRUUHᚐHFWDSULPDU\UROHRIFDYHRODHLQGHWUXVRUVLJQDOOLQJLV QRWNQRZQ7KLVZDVDGGUHVVHGE\PDNLQJDGHWDLOHGIXQFWLRQDOFKDUDFWHUL]DWLRQRI GHWUXVRUFRQWUDFWLOLW\LQZLOGW\SH :7 DQGFDYHROLQGHᚏFLHQW .2 IHPDOHPLFH Material & Methods: 'HWUXVRUVIURPZHHNROG.2DQGFRQJHQLF:7IHPDOH PLFHZHUHH[DPLQHGLQYLWURXVLQJP\RJUDSK\'LXUHVLVZDVHVWLPDWHGE\ZDWHU FRQVXPSWLRQ /HQJWKWHQVLRQ UHODWLRQVKLSV ZHUH JHQHUDWHG DQG FRQWUDFWLOLW\ LQ UHVSRQVH WR WKH PXVFDULQLF DJRQLVW FDUEDFKRO ZDV GHWHUPLQHG DW WKH RSWLPXP OHQJWK / IRU DFWLYH IRUFH :HVWHUQ EORWWLQJ ZDV XVHG WR DVVHVV FRQWHQWV RI FDYHROLQFDYLQDQGPXVFDULQLF0UHFHSWRUV Results: .2PLFHFRQVXPHGVLJQLᚏFDQWO\OHVVZDWHUSHUGD\VXJJHVWLQJUHGXFHG GLXUHVLV7KHEODGGHUWRERG\ZHLJKWUDWLRZDVQRWFKDQJHGLQ.2PLFH7KHSDVVLYH OHQJWKWHQVLRQUHODWLRQVKLSRIWKH.2EODGGHUVZDVOHIWVKLIWHGLQFRPSDULVRQZLWK :7EODGGHUV0D[LPDOFDUEDFKROLQGXFHGIRUFHDW/ZDVLQFUHDVHGLQ.2EODGGHU ZKHQQRUPDOL]HGWRKLJK.FRQWUDFWLRQ Conclusions: *HQHWLFDEODWLRQRIFDYHRODHLQWKHIHPDOHPRXVHOHDGVWRVXEVWDQWLDO IXQFWLRQDOFKDQJHVLQWKHEODGGHUUXOLQJRXWREVWUXFWLRQUHODWHGK\SHUWURSK\DVD FRQWULEXWLQJ IDFWRU 7KH OHIWVKLIW RI WKH SDVVLYH OHQJWKWHQVLRQ UHODWLRQVKLS LQ WKH .2EODGGHUPD\UHᚐHFWDGDSWDWLRQWRUHGXFHGGLXUHVLVRULQFUHDVHGWLVVXHVWLᚎQHVV &DUEDFKRO FRQWUDFWLRQ LV LQFUHDVHG LQ WKH FDYHRODHGHᚏFLHQW IHPDOH GHWUXVRU VXJJHVWLQJWKDWWKHSUHVHQFHRIFDYHRODHVXSSUHVVHVPXVFDULQLFVLJQDOOLQJLQWKH IHPDOHPRXVHEODGGHU
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THE INHIBITORY ROLE OF MELATONIN ON ISOLATED RAT URINARY BLADDER CONTRACTION: THE NOVEL PATHWAY FOR THE PHARMACOLOGICAL MANAGEMENT OF NOCTURIA
.LP7+&KDQJ,++D06.ZRQ<:/HH0<.LP:<.LP-6 +\XQ-60\XQJ6& 1 College of Medicine, Chung-Ang University, Dept. of Urology, Seoul, South Korea, 2Chung-Ang University Hospital, Dept. of Urology, Seoul, South Korea, 3 Gyeongsang National University College of Medicine, Dept. of Urology, Jinju, South Korea Introduction & Objectives: 1RFWXUQDOPHODWRQLQOHYHOVPD\DᚎHFWEODGGHUDFWLYLW\ +HUHZHWHVWHGWKHHᚎHFWVRIPHODWRQLQRQGHWUXVRUPXVFOHFRQWUDFWLRQRILVRODWHG UDWEODGGHU Material & Methods: :HHYDOXDWHGWKHHᚎHFWVRIPHODWRQLQRQWKHFRQWUDFWLRQV LQGXFHGE\SKHQ\OHSKULQH 3( DFHW\OFKROLQH $&K EHWDQHFKRO %&K .&ODQG HOHFWULFDOᚏHOGVWLPXODWLRQ ()6 LQGHWUXVRUVPRRWKPXVFOHVRI6SUDJXH'DZOH\ UDWV 7R GHWHUPLQH WKH PHFKDQLVPV RI WKH LQKLELWRU\ UHVSRQVHV DIWHU PHODWRQLQ PHODWRQLQ SUHWUHDWHG PXVFOH VWULSV ZHUH UHDFWHG ZLWK WKH FDOFLXP FKDQQHO DQWDJRQLVW YHUDSDPLO WKH SRWDVVLXP FKDQQHO EORFNHUV >WHWUDHWK\O DPPRQLXP 7($ DPLQRS\ULGLQH $3 DQG JOLEHQFODPLGH@ GLUHFW YROWDJH GHSHQGHQW FDOFLXPFKDQQHORSHQHU %D\. DQG.1 >VSHFLᚏFFDOFLXPFDOPRGXOLQ GHSHQGHQWNLQDVH,, &D0.,, LQKLELWRU@ Results: 0HODWRQLQ SUHWUHDWPHQW a 0 GRVHGHSHQGHQWO\ GHFUHDVHG WKH FRQWUDFWLOHUHVSRQVHVRI3( a 0 DQG$FK a 0 0HODWRQLQ 0 DOVR EORFNHG FRQWUDFWLRQ LQGXFHG E\ KLJK .&O >.&O@(&) P0 P0 P0 DQG P0 DQG ()6 9HUDSDPLO SRWHQWLDWHG WKH UHOD[DWLRQ UHVSRQVH RIPHODWRQLQWUHDWHGVWULSV 0 EXWRWKHUSRWDVVLXPFKDQQHOEORFNHUVGLGQRW 0HODWRQLQSUHWUHDWPHQWVLJQLᚏFDQWO\GHFUHDVHGWKHFRQWUDFWLOHUHVSRQVHVRI%D\. a0 .1 HQKDQFHGPHODWRQLQLQGXFHGUHOD[DWLRQ Conclusions: 7KH SUHVHQW UHVXOWV VXJJHVW WKDW PHODWRQLQ FDQ LQKLELW EODGGHU VPRRWKPXVFOHVDVDYROWDJHGHSHQGHQWFDOFLXPDQWDJRQLVWLFHᚎHFWDQGFDOPRGXOLQ &D0.,,V\VWHPLQKLELWLRQ&OLQLFDOVLJQLᚏFDQFHZLOOEHVWXGLHGLQWKHIXWXUH
Oral Session 5 PROSTATE CANCER STEM/INITIATING CELLS AND THE MICROENVIRONMENT Sunday, 18 April, 12.15-13.45, Amsterdam Room
278
ROLE OF STROMAL FIBROBLASTS INTERACTION WITH PROSTATIC CANCER CELLS IN ACHIEVEMENT OF METASTATIC PHENOTYPE
6HUQL6*LDQQRQL(0DVLHUL/&DORULQL//DQFLRWWL0,HUDUGL$ 0LQHUYLQL$/DSLQL$&DULQL0&KLDUXJL3 1 University of Florence, Dept. of Urology, Florence, Italy, 2University of Florence, Dept. of Biochemistry, Florence, Italy Introduction & Objectives: 7XPRUDO PLFURHQYLURQPHQW DOVR FDOOHG ಯUHDFWLYH VWURPDರ SOD\V DQ LPSRUWDQW UROH LQ SURVWDWH FDQFHU SURJUHVVLRQ DQG LQ WKH DFKLHYHPHQWRIDPHWDVWDWLFSKHQRW\SH3DUWLFXODUO\DFWLYDWHGᚏEUREODVWV $) ZLWK DQH[QRYRH[SUHVVLRQRI˞60$SURWHLQ VPRRWKPXVFOHDFWLQ DUHVXSSRVHGWR HQKDQFH WXPRUDO FHOO DJJUHVVLYH FKDUDFWHUV DQG PHWDVWDWLF FDSDFLW\ $LP RI RXU VWXG\ZDVWRDQDO\]HWKHELXQLYRFDOLQWHUDFWLRQEHWZHHQᚏEUREODVWVDQGSURVWDWLF FDQFHU FHOOV DQG WR HVWDEOLVK KRZ WXPRUDO FHOOV DFKLHYH WKH DJJUHVVLYH DQG PHWDVWDWLFSKHQRW\SH Material & Methods: :HSHUIRUPHGFXOWXUHVRIᚏEUREODVWVXQWUDQVIRUPHGSURVWDWLF HSLWKHOLDO FHOOV 317 ZHUH FXOWLYDWHG LQ 530, PHGLXP DQG SURVWDWLF FDQFHU