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281. The reliability of automated urinary oestrogen determinations in pregnancy
362
Abstracts
fetal growth retardation (small for date neonates) estrogen clearance remains within thg normal range (202ml/min, SEM = 29.0. n = 15). These findings make it necessary to reconsider the clinical significance of estrogen determinations in pregnant patients with impaired renal function.
279. Quantification of plasma cortisol and urinary hydroxycortisol in mother-infant pairs
277. Comparison of plasma steroid hormone levels in the first trimester of normal human pregnancy with the same levels in pregnant patients with a history of recurrent abortion Courrs,J. R. T.. DODSON. KAY S. and MCEWAN. H. P.. Department of Obstetrics and Gynaecology. Royal Matermty Hospttal. Rottenrow. Glasgow G4 ONA, Scotland
A simple and rapid procedure for the quantitative extraction of plasma cortisol and urinary 68-hydroxycortisol based on an ammonium carbonate-ethyl acetate extraction procedure has been developed; 0.552.0 ml of plasma and lo-11 ml of urine were used. After conversion to methoximeetrimethylsilyl ether derivatives, the steriods were quantified by selective ion detection with a gas chromatograph-mass spectrometer-computer system (LKB 9000-PDP 12). The ions monitored were at ~t/e 605 (M-31) for cortisol and at nrje 693 (M-31) for h/&hydroxycortisol. Both steroids can be detected in nanogram quantities. In one mother-infant pair, the concentration of cortisol was 715 ngml in maternal plasma, 130 ngjml in neonatal plasma (79 11after birth) and 190ng/ml in umbilical venous plasma. The concentration of 6P-hydroxycortisol in the neonatal urine dropped from 33 /rg/34 h on day 2 to 3 pg/24 h on day 15 : the concentrution in a sample of maternal urine collected at d&very was 7pg/ml. Comparable results were obtained with other mother-infant pairs.
During the first trimester, human pregnancy is maintained by the corpus luteum. Thereafter the trophoblast takes over this maintenance. Many cases of recurrent abortion occur between 8 and I? weeks gestation, possibly die to lack of synchronization of the secretions of the corpus luteum and the trophoblast. Progesterone is secreted by both the corpus luteum and the placenta whilst its 17a-hydroxylated derivdtive is secreted by the former, but not the latter. Plasma samples have been collected from two groups of pregnant woman weekly from 5 to 16 weeks after their last menstrual period. These two groups were: (1) apparently normal pregnancies and (2) women with an obstetrical history of recurrent abortion in the first trimester. Using a specific competitive protein binding assay, plasma progesterone and plasma 17r-hydroxyprogesterone levels have been determined in all samples. These results show the functional life of the corpus luteum of pregnancy in “normal” pregnancy and comparison of the levels in the two groups suggest possible endocrinological explanations for recurrent abortion in the women with bad obstetrical histories.
278. Plasma levels of unconjugated estrone, estradiol and estriol in early pregnancy. Correlation with HCS in the same subjects DE HERTOGH, R., THOMAS. K. and VANDERHEYDEN, I., Hopital Saint Pierre. University of Louvain. Belgium Unconjugated estrone. estradiol and estriol (separated on Sephadex LH,, column chromatography). and HCS levels were determined by radioimmunoassay in the same plasma samples of normal pregnant women. The fluctuations of these hormonal parameters were not parallel. Estradiol and estrone increased slowly in the first weeks after implantation. but showed a steeper increase at about the ninth week. Estriol increase was mild up to the 10th week; the levels increased markedly by the 11th week. The ratio of estriol to estradiol also increased significantly; HCS showed a steep increase to the ninth week, and the ratio of HCS to estradiol increased. The ratio of HCS to estriol did not increase significantly in the early weeks of pregnancy but decreased, steeply after the 12th week. The results are interpreted as reflecting the sequential modifications in hormone production which takes place in early pregnancy: the relative contribution of the corpus luteum to estrogen production (mostly estradiol) becomes of less imuortance bv the ninth week in the presence of the growing placental function (estrogens and HCS). By the 12th week, the foetal contribution to placental estrogen procursors becomes evident by the steep increase of estriol.
6/J-
HORNING, M. G.. LAU. S. S., HUN<;. A.. SIILL~VFI I..
W. G. and HILL, R. M., Instttute for Ltptd Research and Department of Pediatrics, Baylor College of Medicine, Houston. Texas. 77025. (1.S.A.
280. Estriol levels in human amniotic fluid PEREZ-LOPEZ, F. R.. RONCERO. M. C.. CANAS. E..t DAVI.E..~XI~SALTUKOGLLI, A.. Human Reproduction Research Unit, Hopital St. Pierre, Universite Libre de Bruxelles, Brussels, Belgium. and tDepartment of Obstetrics and Gynaecology, Hospital Climco y Provincial, Universidad de Barcelona. Barcelona. Spain For the monitoring of fetal well-being durtng pregnancy a great number of methods have been proposed. The determination of estriol is one useful way to follow the condition of the fetus: however. effective evaluation of urinary or serum estriol requires an understanding of multiple variables, many of which are totally unrelated to fetal status. We have therefore studied the estriol content of amniotic fluid (AF). Liquor samples were obtained by amniocentesis or by amniotomy during labour. All specimens were stored at - 20 ~C unttl assayed in triplicate by a radioimmunoassay method. All subjects and children were normal throughout pregnancy and delivery. The results show a progressive increase of the mean levels during the third trimester of pregnancy. from some 100 ng,iml in week 30 to 908ng/ml in week 40. The values remain around 900ng/ml in week 41 and then decrease to 630 ngi’ml in week 41. It is likely that the estriol content of AF is determined primarily by the metabolic at&or excretory function ofthe fetoplacental unit, so alterations in AF estriol levels can reflect the fetal status. 281. The reliability of automated urinary ocstrogen determinations in pregnancy MOSCROP, K. H., ANTCLIFF. A. C.. BRA~~NSBERC~, H.. JAMS. V. H. T.. GOUDIE. J. H. and B~IRNFI‘r. D., St. John’s Hospital. Chelmsford, St. Mary’s Hospital. London W.7. and City Hospital. St. Albans. England Automated analysis of urinary for monitoring foetal-placental
oestrogens is now used widely well-being. Results obtained
Abstracts
fetal growth retardation (small for date neonates) estrogen clearance remains within thg normal range (202ml/min, SEM = 29.0. n = 15). These findings make it necessary to reconsider the clinical significance of estrogen determinations in pregnant patients with impaired renal function.
279. Quantification of plasma cortisol and urinary hydroxycortisol in mother-infant pairs
277. Comparison of plasma steroid hormone levels in the first trimester of normal human pregnancy with the same levels in pregnant patients with a history of recurrent abortion Courrs,J. R. T.. DODSON. KAY S. and MCEWAN. H. P.. Department of Obstetrics and Gynaecology. Royal Matermty Hospttal. Rottenrow. Glasgow G4 ONA, Scotland
A simple and rapid procedure for the quantitative extraction of plasma cortisol and urinary 68-hydroxycortisol based on an ammonium carbonate-ethyl acetate extraction procedure has been developed; 0.552.0 ml of plasma and lo-11 ml of urine were used. After conversion to methoximeetrimethylsilyl ether derivatives, the steriods were quantified by selective ion detection with a gas chromatograph-mass spectrometer-computer system (LKB 9000-PDP 12). The ions monitored were at ~t/e 605 (M-31) for cortisol and at nrje 693 (M-31) for h/&hydroxycortisol. Both steroids can be detected in nanogram quantities. In one mother-infant pair, the concentration of cortisol was 715 ngml in maternal plasma, 130 ngjml in neonatal plasma (79 11after birth) and 190ng/ml in umbilical venous plasma. The concentration of 6P-hydroxycortisol in the neonatal urine dropped from 33 /rg/34 h on day 2 to 3 pg/24 h on day 15 : the concentrution in a sample of maternal urine collected at d&very was 7pg/ml. Comparable results were obtained with other mother-infant pairs.
During the first trimester, human pregnancy is maintained by the corpus luteum. Thereafter the trophoblast takes over this maintenance. Many cases of recurrent abortion occur between 8 and I? weeks gestation, possibly die to lack of synchronization of the secretions of the corpus luteum and the trophoblast. Progesterone is secreted by both the corpus luteum and the placenta whilst its 17a-hydroxylated derivdtive is secreted by the former, but not the latter. Plasma samples have been collected from two groups of pregnant woman weekly from 5 to 16 weeks after their last menstrual period. These two groups were: (1) apparently normal pregnancies and (2) women with an obstetrical history of recurrent abortion in the first trimester. Using a specific competitive protein binding assay, plasma progesterone and plasma 17r-hydroxyprogesterone levels have been determined in all samples. These results show the functional life of the corpus luteum of pregnancy in “normal” pregnancy and comparison of the levels in the two groups suggest possible endocrinological explanations for recurrent abortion in the women with bad obstetrical histories.
278. Plasma levels of unconjugated estrone, estradiol and estriol in early pregnancy. Correlation with HCS in the same subjects DE HERTOGH, R., THOMAS. K. and VANDERHEYDEN, I., Hopital Saint Pierre. University of Louvain. Belgium Unconjugated estrone. estradiol and estriol (separated on Sephadex LH,, column chromatography). and HCS levels were determined by radioimmunoassay in the same plasma samples of normal pregnant women. The fluctuations of these hormonal parameters were not parallel. Estradiol and estrone increased slowly in the first weeks after implantation. but showed a steeper increase at about the ninth week. Estriol increase was mild up to the 10th week; the levels increased markedly by the 11th week. The ratio of estriol to estradiol also increased significantly; HCS showed a steep increase to the ninth week, and the ratio of HCS to estradiol increased. The ratio of HCS to estriol did not increase significantly in the early weeks of pregnancy but decreased, steeply after the 12th week. The results are interpreted as reflecting the sequential modifications in hormone production which takes place in early pregnancy: the relative contribution of the corpus luteum to estrogen production (mostly estradiol) becomes of less imuortance bv the ninth week in the presence of the growing placental function (estrogens and HCS). By the 12th week, the foetal contribution to placental estrogen procursors becomes evident by the steep increase of estriol.
6/J-
HORNING, M. G.. LAU. S. S., HUN<;. A.. SIILL~VFI I..
W. G. and HILL, R. M., Instttute for Ltptd Research and Department of Pediatrics, Baylor College of Medicine, Houston. Texas. 77025. (1.S.A.
280. Estriol levels in human amniotic fluid PEREZ-LOPEZ, F. R.. RONCERO. M. C.. CANAS. E..t DAVI.E..~XI~SALTUKOGLLI, A.. Human Reproduction Research Unit, Hopital St. Pierre, Universite Libre de Bruxelles, Brussels, Belgium. and tDepartment of Obstetrics and Gynaecology, Hospital Climco y Provincial, Universidad de Barcelona. Barcelona. Spain For the monitoring of fetal well-being durtng pregnancy a great number of methods have been proposed. The determination of estriol is one useful way to follow the condition of the fetus: however. effective evaluation of urinary or serum estriol requires an understanding of multiple variables, many of which are totally unrelated to fetal status. We have therefore studied the estriol content of amniotic fluid (AF). Liquor samples were obtained by amniocentesis or by amniotomy during labour. All specimens were stored at - 20 ~C unttl assayed in triplicate by a radioimmunoassay method. All subjects and children were normal throughout pregnancy and delivery. The results show a progressive increase of the mean levels during the third trimester of pregnancy. from some 100 ng,iml in week 30 to 908ng/ml in week 40. The values remain around 900ng/ml in week 41 and then decrease to 630 ngi’ml in week 41. It is likely that the estriol content of AF is determined primarily by the metabolic at&or excretory function ofthe fetoplacental unit, so alterations in AF estriol levels can reflect the fetal status. 281. The reliability of automated urinary ocstrogen determinations in pregnancy MOSCROP, K. H., ANTCLIFF. A. C.. BRA~~NSBERC~, H.. JAMS. V. H. T.. GOUDIE. J. H. and B~IRNFI‘r. D., St. John’s Hospital. Chelmsford, St. Mary’s Hospital. London W.7. and City Hospital. St. Albans. England Automated analysis of urinary for monitoring foetal-placental
oestrogens is now used widely well-being. Results obtained