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3-D cytoarchitectonic probabilistic map of human medial prefrontal cortex
Nemoimage
13, Number
6, 2001, Part 2 of 2 Parts 1 D E al@
METHODS
3-D CYTOARCHITECTONIC E.J. Sanz Arigita*,
- ANALYSIS
PROBABILISTIC MAP OF HUMAN PREFRONTAL CORTEX.
MEDIAL
K. de Vos*, C.W. Pool*, Karl ZillestS, H.B.M. Uylings*!j
*Netherlands Institute for Brain Research, Amsterdam, The Netherlands TC. & 0. Vogt Institute for Brain Research, Diisseldo$
Germany
Ilnst. Medicine, Jiilich Research Center, Jiilich, Germany §Department of Anatomy, Vrije Universiteit, Amsterdam, The Netherlands Subject Data originated by functional imaging techniques is usually represented in population maps integrating cerebral metabolic task-related changes over a number of subjects; this functional information is then correlated with structural data. However, the anatomical maps of the brain used as reference are, but for a few exceptions (l), based on single subject studies. As a result, the natural functional intersubject variability intrinsic to the functional studies (2) can not be related with its structural counterpart, therefore affecting the accuracy of the structural interpretation of functional data-sets. Our study attempts to define the cytoarchitectonic parcellation of the medial prefrontal cortex (including anterior cingulate and ventromedial prefrontal cortices -mPFC-) over a number of subjects, based on a combination of qualitative criteria (characterizing relative prominence of cortical layers and somatic size/shape) and quantitative (densitometric) measurements. Methods We have studied five human brains without neurological or psychiatric diagnoses. Postmortem MRIs were taken before brains were paraffin embedded, cut and stained with Gallyas modification for neuronal cell bodies. Every mPFC area was defined by a specific set of cytoarchitectonic criteria (3) in combination with quantitative measurements (4). The borders between mPFC areas were delineated on a series of coronal sections and, by an affine transformation, superimposed onto MRI images and reconstructed in 3-D. These individual 3-D cytoarchitectonic maps were then transformed by elastic deformation (5) to a reference brain (6) and the results pooled to construct the population/probabilistic structural map. Results Our analyses demonstrate a very high variability in the relative extent of the mPFC areas that seems to be independent of the general topography of the region. The analysis of the population maps shows that this variability is not constant through the whole region, but rather it differs depending on the area examined. The result of the combined qualitative and quantitative microstructural analysis of the human cerebral cortex reveals a rostro-caudal gradual change in cytoarchitecture. Further, there is an structural inter-subject variability within the areas examined. Our data provides evidence of the actual extent of cingulate area 32 which terminates rostra1 to the genu of the corpus callosum. Conclusions We illustrate the location and extent of the mPFC structural maps. This approach allows the combination functional meta-analysis of the mPFC.
areas both in individual 3-D reconstructions of both anatomical and functional data-sets
References 1. 2. 3. 4. 5. 6.
Rajkowska G. and GoldmanRakic, P.S. (1995b); Posner MI. and Raiche M.E. (1994). Sanz Arigita E.J. et a1.(1999). Schleicher A. and Zilles K. (1990). Schormann T. and Zilles K. (1998). Roland P.E. and Zilles, K. (1998).
Supported
by EU grants
BI04CT96-0177
Geyer
S. et al. (1998).
and BI04CT97-5128
S236
and population/probabilistic to perform detailed structural/
13, Number
6, 2001, Part 2 of 2 Parts 1 D E al@
METHODS
3-D CYTOARCHITECTONIC E.J. Sanz Arigita*,
- ANALYSIS
PROBABILISTIC MAP OF HUMAN PREFRONTAL CORTEX.
MEDIAL
K. de Vos*, C.W. Pool*, Karl ZillestS, H.B.M. Uylings*!j
*Netherlands Institute for Brain Research, Amsterdam, The Netherlands TC. & 0. Vogt Institute for Brain Research, Diisseldo$
Germany
Ilnst. Medicine, Jiilich Research Center, Jiilich, Germany §Department of Anatomy, Vrije Universiteit, Amsterdam, The Netherlands Subject Data originated by functional imaging techniques is usually represented in population maps integrating cerebral metabolic task-related changes over a number of subjects; this functional information is then correlated with structural data. However, the anatomical maps of the brain used as reference are, but for a few exceptions (l), based on single subject studies. As a result, the natural functional intersubject variability intrinsic to the functional studies (2) can not be related with its structural counterpart, therefore affecting the accuracy of the structural interpretation of functional data-sets. Our study attempts to define the cytoarchitectonic parcellation of the medial prefrontal cortex (including anterior cingulate and ventromedial prefrontal cortices -mPFC-) over a number of subjects, based on a combination of qualitative criteria (characterizing relative prominence of cortical layers and somatic size/shape) and quantitative (densitometric) measurements. Methods We have studied five human brains without neurological or psychiatric diagnoses. Postmortem MRIs were taken before brains were paraffin embedded, cut and stained with Gallyas modification for neuronal cell bodies. Every mPFC area was defined by a specific set of cytoarchitectonic criteria (3) in combination with quantitative measurements (4). The borders between mPFC areas were delineated on a series of coronal sections and, by an affine transformation, superimposed onto MRI images and reconstructed in 3-D. These individual 3-D cytoarchitectonic maps were then transformed by elastic deformation (5) to a reference brain (6) and the results pooled to construct the population/probabilistic structural map. Results Our analyses demonstrate a very high variability in the relative extent of the mPFC areas that seems to be independent of the general topography of the region. The analysis of the population maps shows that this variability is not constant through the whole region, but rather it differs depending on the area examined. The result of the combined qualitative and quantitative microstructural analysis of the human cerebral cortex reveals a rostro-caudal gradual change in cytoarchitecture. Further, there is an structural inter-subject variability within the areas examined. Our data provides evidence of the actual extent of cingulate area 32 which terminates rostra1 to the genu of the corpus callosum. Conclusions We illustrate the location and extent of the mPFC structural maps. This approach allows the combination functional meta-analysis of the mPFC.
areas both in individual 3-D reconstructions of both anatomical and functional data-sets
References 1. 2. 3. 4. 5. 6.
Rajkowska G. and GoldmanRakic, P.S. (1995b); Posner MI. and Raiche M.E. (1994). Sanz Arigita E.J. et a1.(1999). Schleicher A. and Zilles K. (1990). Schormann T. and Zilles K. (1998). Roland P.E. and Zilles, K. (1998).
Supported
by EU grants
BI04CT96-0177
Geyer
S. et al. (1998).
and BI04CT97-5128
S236
and population/probabilistic to perform detailed structural/