- Email: [email protected]
3.6. PGM1-fokussierung an frischen menschlichen körpergeweben und nach lagerung
251
References 1 G. F. Sensabaugh and D. Grim, J. Forensic Sci., 23 (1978) 106 - 114. 2 P. Vihko, K. Mattila and C. Ehnholm, Am. J. Clin. Pathol., 75 (1981)
3.5. BLOOD
GROUP
SUBSTANCES
IN TOOTH
219
- 220.
DENTINE
D. K. Whittaker’ and T. J. Rothwell’ (lDenta1 Hospital, Heath, Cardiff, S. Wales, Gt. Britain; and 2Home Office Forensic Science Laboratory, Usk Road, Chepstow, Gwent, Gt. Britain) The scope of forensic dentistry has expanded in recent years owing largely to the increase in international travel and to the recognition of its importance in mass disasters. It is well recognised that teeth constitute the most indestructible part of the body, and in some air disasters dental evidence alone has been responsible for identification in up to 40% of the victims. The dentine of a tooth constitutes a living hard tissue since it is permeated by many protoplasmic processes extending from cells in the pulp. In view of this it was decided to carry out work to determine whether blood group antigens or other factors could be detected within the dentine itself. Teeth extracted under general anaesthesia were split longitudinally and both the outer layers and the pulpal dentine removed by grinding. This procedure left a core of dentine uncontaminated with soft tissue. The dentine was crushed into a powder in a hammer mill and soluble blood group substances extracted into a suitable medium. The extracts were tested against species-specific antisera using an immunoelectrophoretic technique. A human reaction was obtained from most of the samples. The extracts were also tested for the presence of phosphoglucomutase (PGM) isoenzymes by electrophoretic methods and again a number of the samples yielded positive results. Tests for other isoenzymes, e.g. adenylate kinase (AK), and also for the ABO antigens, were negative. These results indicate that even after vigorous cleaning of the dentine of contaminating soft tissues, there remained sufficient cellular material within the tubules to allow the determination of the species and the detection of certain blood groups. These tests may be of value in the investigation of mass disasters and also in the examination of small fragments of tooth sometimes found, for example, in foodstuffs. The project was supported by a grant from the Home Office Central Research Establishment at Aldermaston. PGM1-FOKUSSIERUNG UND NACH LAGERUNG
3.6.
AN
FRISCHEN
MENSCHLICHEN
K6RPERGEWEBEN
E. Tutsch-Bauer, M. Oya und H. D. TrSger (Institut fur Rechtsmedizin der Universitat Munchen, Frauenlobstrasse 7a, 8000 Miinchen 2, B.R.D.)
References 1 G. F. Sensabaugh and D. Grim, J. Forensic Sci., 23 (1978) 106 - 114. 2 P. Vihko, K. Mattila and C. Ehnholm, Am. J. Clin. Pathol., 75 (1981)
3.5. BLOOD
GROUP
SUBSTANCES
IN TOOTH
219
- 220.
DENTINE
D. K. Whittaker’ and T. J. Rothwell’ (lDenta1 Hospital, Heath, Cardiff, S. Wales, Gt. Britain; and 2Home Office Forensic Science Laboratory, Usk Road, Chepstow, Gwent, Gt. Britain) The scope of forensic dentistry has expanded in recent years owing largely to the increase in international travel and to the recognition of its importance in mass disasters. It is well recognised that teeth constitute the most indestructible part of the body, and in some air disasters dental evidence alone has been responsible for identification in up to 40% of the victims. The dentine of a tooth constitutes a living hard tissue since it is permeated by many protoplasmic processes extending from cells in the pulp. In view of this it was decided to carry out work to determine whether blood group antigens or other factors could be detected within the dentine itself. Teeth extracted under general anaesthesia were split longitudinally and both the outer layers and the pulpal dentine removed by grinding. This procedure left a core of dentine uncontaminated with soft tissue. The dentine was crushed into a powder in a hammer mill and soluble blood group substances extracted into a suitable medium. The extracts were tested against species-specific antisera using an immunoelectrophoretic technique. A human reaction was obtained from most of the samples. The extracts were also tested for the presence of phosphoglucomutase (PGM) isoenzymes by electrophoretic methods and again a number of the samples yielded positive results. Tests for other isoenzymes, e.g. adenylate kinase (AK), and also for the ABO antigens, were negative. These results indicate that even after vigorous cleaning of the dentine of contaminating soft tissues, there remained sufficient cellular material within the tubules to allow the determination of the species and the detection of certain blood groups. These tests may be of value in the investigation of mass disasters and also in the examination of small fragments of tooth sometimes found, for example, in foodstuffs. The project was supported by a grant from the Home Office Central Research Establishment at Aldermaston. PGM1-FOKUSSIERUNG UND NACH LAGERUNG
3.6.
AN
FRISCHEN
MENSCHLICHEN
K6RPERGEWEBEN
E. Tutsch-Bauer, M. Oya und H. D. TrSger (Institut fur Rechtsmedizin der Universitat Munchen, Frauenlobstrasse 7a, 8000 Miinchen 2, B.R.D.)