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389. Effect of ecdysterone on cyclic nucleotide levels in mouse plasma
892
Abstracts
\amplch from an adrcnalectomizcd-ovariectomized cow wcrc studied in order to conlirm that the progesterone 111~hc ovariectomized cows wus from the adrenal glands. The cow investigated wus adrenalectomized a~ an age of 6 months and produced 3 calves prior IO bilateral ovariectomy. ;II the age of 52 months. AI the time of sampling the animal was in good health. was receiving no rcplacemcnt therapy and was not cycling. Blood samples a~ Intervals of 461 days and 494 days posl ovariectomy were used li)r cstimution. using an ether extract of the plasma. paper chromatography (two rcplicntes on silica-gel loaded paper) and linal quantttation by gas liquid-chromatography. Two samples bcrc estimated. one in duplicate and the other In yuadruplicatc. Both the samples unexpectedly revealed IIIC prLIscncc or progesterone and the quantity estimated varied from 0.8 ngsml IO 3.7 ng,ml. The level of progesterone in the systemic plasma of the adrenalectomizedov~lricctomized cow studied was similar IO that found m :III ~aricctomi~ed CON 50 days post-ovaricctomy. The prclcncc 0r progesterone In this cow has not been explained.
388. Elkct of bormoaal tberapy on plasma progeskroae and l’lb_atndiol levels in cows sulking from cystic ovarian degeneration DISIT. V. P.. DRA~TA. D..* STROE. E..* KHAR. S. K. and SIWIU. FI... *Institute of Endocrinology. 34 Bd. Aviatorilor. 71270 Bucharest. and Faculty of Veterinary Medicine. 105 Spliaul lndependentei 7OOO Bucharest. Romania One hundred and three cows having single or multiple cysts on one or both ovaries were treated with LH-RF. LH and progesterone and dexamethasone. A single intravenous injection of 115 mg progesterone and 3000 i.u. LH was found to be the most effective treatment in this study. giving X3.5”,, positive results followed by dexamethasone W,,. In the latter two treatments in 7 I .o”,, and LH-RF some cases the same dose had to be repeated. The clinical observations were augmented with the levels of plasma progesterone and 17@stradiol. determined by RIA. Based on rectal examination and plasma progesterone level. the cysis were classified as lutcal cysts or follicular cysts. the
former showing comparatively higher plasma progesterone levels than the latter. The plasma I7@tradiol levels were reduced along with the size of the cysts by the efficient hormonal treatment. The efficiency and economic aspects of the various treatments are discussed.
389. Elkt of ecdysterone on cyclic nucleotide levcb in mouse plasma CATALAN. R. E.. ARAGONES. M. D. and MARTINEZ. A. M.. Departamento de Bioquimica. Colegio Universitario Arcos de Jal6n. Arcos de Jal6n. s/n. Madrid-17. Spain The heterophylic action of invertebrate hormones on metabolic and molecular events in vertebrates has been demonstrated in several cases although the exact mechanism of action is not completely understood. The heterophylic action of some insect-metamorphosing steroids has been described. indicating that a certain degree of regulation by this steroid on protein. lipid and carbohydrate metabolism takes place. It is the aim of the present work to examine the efTect of ecdysterone on CAMP and cGMP levels in mouse plasma. Male mice (18-2Og) were injected intraperitoneally at a dose of 10figper mouse. Mice were killed by decapitation and nucleotides were determined on blood samples. CAMP was measured by a competitive binding protein assay. cGMP was measured by a radioimmunoassay. Levels of CAMP showed a sharp decrease after 4Omin. These changes coincide with opposite variations in cGMP levels. Results are discussed in terms of the variations exhibited by glucose after treatment with hyperglycemic agents and ecdysterone and lead us to assume that ecdysterone action on carbohydrate metabolism may be mediated by nucleotide levels. Preliminary data obtained in liver showed similar variations of cyclic nucleotide levels. Our results suggest that some heterophylic actions of ecdysterone in mammals are probably mediated through the CAMP system. At the same time these data give additional support to the relationship between ecdysterone and the CAMP-protein kinase system described in invertebrates and also to the synchronous variation undergone by CAMP and cGMP described in several biological systems.
Abstracts
\amplch from an adrcnalectomizcd-ovariectomized cow wcrc studied in order to conlirm that the progesterone 111~hc ovariectomized cows wus from the adrenal glands. The cow investigated wus adrenalectomized a~ an age of 6 months and produced 3 calves prior IO bilateral ovariectomy. ;II the age of 52 months. AI the time of sampling the animal was in good health. was receiving no rcplacemcnt therapy and was not cycling. Blood samples a~ Intervals of 461 days and 494 days posl ovariectomy were used li)r cstimution. using an ether extract of the plasma. paper chromatography (two rcplicntes on silica-gel loaded paper) and linal quantttation by gas liquid-chromatography. Two samples bcrc estimated. one in duplicate and the other In yuadruplicatc. Both the samples unexpectedly revealed IIIC prLIscncc or progesterone and the quantity estimated varied from 0.8 ngsml IO 3.7 ng,ml. The level of progesterone in the systemic plasma of the adrenalectomizedov~lricctomized cow studied was similar IO that found m :III ~aricctomi~ed CON 50 days post-ovaricctomy. The prclcncc 0r progesterone In this cow has not been explained.
388. Elkct of bormoaal tberapy on plasma progeskroae and l’lb_atndiol levels in cows sulking from cystic ovarian degeneration DISIT. V. P.. DRA~TA. D..* STROE. E..* KHAR. S. K. and SIWIU. FI... *Institute of Endocrinology. 34 Bd. Aviatorilor. 71270 Bucharest. and Faculty of Veterinary Medicine. 105 Spliaul lndependentei 7OOO Bucharest. Romania One hundred and three cows having single or multiple cysts on one or both ovaries were treated with LH-RF. LH and progesterone and dexamethasone. A single intravenous injection of 115 mg progesterone and 3000 i.u. LH was found to be the most effective treatment in this study. giving X3.5”,, positive results followed by dexamethasone W,,. In the latter two treatments in 7 I .o”,, and LH-RF some cases the same dose had to be repeated. The clinical observations were augmented with the levels of plasma progesterone and 17@stradiol. determined by RIA. Based on rectal examination and plasma progesterone level. the cysis were classified as lutcal cysts or follicular cysts. the
former showing comparatively higher plasma progesterone levels than the latter. The plasma I7@tradiol levels were reduced along with the size of the cysts by the efficient hormonal treatment. The efficiency and economic aspects of the various treatments are discussed.
389. Elkt of ecdysterone on cyclic nucleotide levcb in mouse plasma CATALAN. R. E.. ARAGONES. M. D. and MARTINEZ. A. M.. Departamento de Bioquimica. Colegio Universitario Arcos de Jal6n. Arcos de Jal6n. s/n. Madrid-17. Spain The heterophylic action of invertebrate hormones on metabolic and molecular events in vertebrates has been demonstrated in several cases although the exact mechanism of action is not completely understood. The heterophylic action of some insect-metamorphosing steroids has been described. indicating that a certain degree of regulation by this steroid on protein. lipid and carbohydrate metabolism takes place. It is the aim of the present work to examine the efTect of ecdysterone on CAMP and cGMP levels in mouse plasma. Male mice (18-2Og) were injected intraperitoneally at a dose of 10figper mouse. Mice were killed by decapitation and nucleotides were determined on blood samples. CAMP was measured by a competitive binding protein assay. cGMP was measured by a radioimmunoassay. Levels of CAMP showed a sharp decrease after 4Omin. These changes coincide with opposite variations in cGMP levels. Results are discussed in terms of the variations exhibited by glucose after treatment with hyperglycemic agents and ecdysterone and lead us to assume that ecdysterone action on carbohydrate metabolism may be mediated by nucleotide levels. Preliminary data obtained in liver showed similar variations of cyclic nucleotide levels. Our results suggest that some heterophylic actions of ecdysterone in mammals are probably mediated through the CAMP system. At the same time these data give additional support to the relationship between ecdysterone and the CAMP-protein kinase system described in invertebrates and also to the synchronous variation undergone by CAMP and cGMP described in several biological systems.