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3H-paroxetine binding and serotonin2 (5-HT2) receptor binding in the blood platelets of posttraumatic stress disorder (PTSD)
106A
BIOL PSYCHIATRY
1992;31:6!A-252A
Studies Related to the Serotonin System
0.05). In summary, although we did not find group differences, our preliminary data are consistent with a possible relationship of PTSD subtypes to serotonin dysfunction.
104 3H-PAROXETINE BINDING AND SEROTONIN2 (5-HT2)
RECEPTOR BINDING IN THE BLOOD PLATELETS OF POSTTRAUMATIC STRESS DISORDER (PTSD) Ramesh C. Arora, Chris Fichtner, John W. Crayton Loyola Stritch School of Medicine and Hines VA Hospital, Hines, IL 60141. Serotonergic abnormalities have been reported in the blood platelets (BPL) of depressed and panic disorder patients. We have now studied serotonin (5-HT) uptake and 5-HT2 binding in the blood platelets of PTSD patients. 5-HT uptake was determined by studying 3H-Paror~efine binding to BPL. 5-HT2 receptor binding was studied using 3H-LSD. Bmax(maximum number of binding sites) of ~H-Paroxetine binding was significantly decreased (p < 0.005) in PTSD patients (n = 12) compared with NC (n = 11). Mean (__.SD) Bmaxvalues in PTSD and normal controls (NC) were 838.2 _ 202.2 and 1165.1 ± 287.7 fmols/mg protein, respectively. There was also a trend for lower Kd in PTSD patients than NC. There was no difference in Bm,~ between PTSD patients and PTSD patients with major depression (n = 4). Bm~xof 5-HT2 binding was also significantly decreased (p < 0.05) in the blood platelets of PTSD patients compared with NC (n = 5). No difference in K,j was observed betw~ta~ me two groups. These results suggest a dysfunction of 5-HT in PTSD patients.
105 ABNORMAL BEHAVIORAL RESPONSES TO FENFLURAMINE
IN PATIENTS WITH AFFECTIVE AND PERSONALITY DISORDERS: CORRELATION WITH INCREASED SEROTONERGIC RESPONSIVITY Joyce E. Myers, Tammy Mieczkowski, James Perel, Donna Abbondanza, Thomas B. Cooper, J. John Mann University of Pittsburgh, Western Psychiatric Institute and Clinic, Pittsburgh, PA 15213. Scrotonergic responsivity was assessed in 20 psychiatric patients by the prolactin response to a fenfluramine challenge test. During the fenfluramine challenge, six of 20 patients (30%) spontaneously reported psychopathologic reactions which included increased anxiety/agitation, psychotic symptoms, illusions, mood elevation, and anergia. The time of peak behavioral symptoms (2.5 ± 0.8 hr) corresponded closely to the time of peak increase in prolactin levels (3.0 _ !. ! hr). Abnormal behavioral responders had statistically significant greater increases in prolactin ! through 4 hr after fenfluramine when compared with normal responders. Patients who dt, veloped an abnormal psychopathologic response to fenfluramine were characterized by higher levels of anx;cty a,d agitation at the time of adm's~ion to the hospital but otherwise were not distinguishable on the basis of severit~ of other psychiatric symptoms. This study suggests that increased serotonergic transmission may trigger anxiety, psychosis, and mood elevation in specific vulnerable individuals, whereas other patients with similar psychiatric illnesses are not affected.
106 INCREASED PLATELET 5-HT IN DEPRESSED PATIENTS WITH
SUICIDAL BEHAVIOR Ru-Band Lu, Huei-Chen Ko, Yaw Horng, Su-Chen Li Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, R.O.C. and National Cheng Kung University Medical College, Tainan, Taiwan, R.O.C. This study was designed to explore whether stress, serotonergic, and noradrenergic functions interact in the psychopathogenesis of suicidal behavior in depressed patients. Eighty-four patients met DSM-III-R criteria for major depressive episodes. Among them, five patients who had attempted suicide had significantly
BIOL PSYCHIATRY
1992;31:6!A-252A
Studies Related to the Serotonin System
0.05). In summary, although we did not find group differences, our preliminary data are consistent with a possible relationship of PTSD subtypes to serotonin dysfunction.
104 3H-PAROXETINE BINDING AND SEROTONIN2 (5-HT2)
RECEPTOR BINDING IN THE BLOOD PLATELETS OF POSTTRAUMATIC STRESS DISORDER (PTSD) Ramesh C. Arora, Chris Fichtner, John W. Crayton Loyola Stritch School of Medicine and Hines VA Hospital, Hines, IL 60141. Serotonergic abnormalities have been reported in the blood platelets (BPL) of depressed and panic disorder patients. We have now studied serotonin (5-HT) uptake and 5-HT2 binding in the blood platelets of PTSD patients. 5-HT uptake was determined by studying 3H-Paror~efine binding to BPL. 5-HT2 receptor binding was studied using 3H-LSD. Bmax(maximum number of binding sites) of ~H-Paroxetine binding was significantly decreased (p < 0.005) in PTSD patients (n = 12) compared with NC (n = 11). Mean (__.SD) Bmaxvalues in PTSD and normal controls (NC) were 838.2 _ 202.2 and 1165.1 ± 287.7 fmols/mg protein, respectively. There was also a trend for lower Kd in PTSD patients than NC. There was no difference in Bm,~ between PTSD patients and PTSD patients with major depression (n = 4). Bm~xof 5-HT2 binding was also significantly decreased (p < 0.05) in the blood platelets of PTSD patients compared with NC (n = 5). No difference in K,j was observed betw~ta~ me two groups. These results suggest a dysfunction of 5-HT in PTSD patients.
105 ABNORMAL BEHAVIORAL RESPONSES TO FENFLURAMINE
IN PATIENTS WITH AFFECTIVE AND PERSONALITY DISORDERS: CORRELATION WITH INCREASED SEROTONERGIC RESPONSIVITY Joyce E. Myers, Tammy Mieczkowski, James Perel, Donna Abbondanza, Thomas B. Cooper, J. John Mann University of Pittsburgh, Western Psychiatric Institute and Clinic, Pittsburgh, PA 15213. Scrotonergic responsivity was assessed in 20 psychiatric patients by the prolactin response to a fenfluramine challenge test. During the fenfluramine challenge, six of 20 patients (30%) spontaneously reported psychopathologic reactions which included increased anxiety/agitation, psychotic symptoms, illusions, mood elevation, and anergia. The time of peak behavioral symptoms (2.5 ± 0.8 hr) corresponded closely to the time of peak increase in prolactin levels (3.0 _ !. ! hr). Abnormal behavioral responders had statistically significant greater increases in prolactin ! through 4 hr after fenfluramine when compared with normal responders. Patients who dt, veloped an abnormal psychopathologic response to fenfluramine were characterized by higher levels of anx;cty a,d agitation at the time of adm's~ion to the hospital but otherwise were not distinguishable on the basis of severit~ of other psychiatric symptoms. This study suggests that increased serotonergic transmission may trigger anxiety, psychosis, and mood elevation in specific vulnerable individuals, whereas other patients with similar psychiatric illnesses are not affected.
106 INCREASED PLATELET 5-HT IN DEPRESSED PATIENTS WITH
SUICIDAL BEHAVIOR Ru-Band Lu, Huei-Chen Ko, Yaw Horng, Su-Chen Li Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, R.O.C. and National Cheng Kung University Medical College, Tainan, Taiwan, R.O.C. This study was designed to explore whether stress, serotonergic, and noradrenergic functions interact in the psychopathogenesis of suicidal behavior in depressed patients. Eighty-four patients met DSM-III-R criteria for major depressive episodes. Among them, five patients who had attempted suicide had significantly