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4357320 Infectious bronchitis vaccine for poultry
143
PATENT ABSTRACTS
4358538
4357422
MYCOBACTERIUM FORTUITUM MUTANT
M E T H O D OF ENHANCING INTERFERON P R O D U C T I O N
Merle G. Wovcha. Kevin E. Brooks, assigned to The Upjohn Company
Donald Giard, Robert J. Fleischaker, assigned to Massachusetts Institute of Technology,
Mutant which is used in a novel microbiological process to selectively transform steroids having 17-alkyl side chains of from 2 to 10 carbon atoms, inclusive, to yield sole transformed product. AD is a valuable intermediate to make useful steroids.
4358535
A method for enhancing the production of interferon from normal human diploid fibroblast cells is disclosed. In this method, a distinct interferon-production phase is established in which the temperature is initially elevated for a brief period followed by a reduction in temperature for the balance of the interferon-production phase.
SPECIFIC DNA PROBES IN DIAGNOSTIC MICROBIOLOGY Stanley Falkow, Stephen L. Moseley, assigned to Board of Regents of the University of Washington Method and compositions for infectious disease diagnosis and epidemiology involving labeled nucleotide probes complementary to nucleic acid coding for a characteristic pathogen product. Clinical isolates are cultivated, expanding the number of microorganisms, the resulting colonies lysed, the genome normally denatured and then fixed. Alternatively, clinical samples (stool, sputum, pus, etc.) are spotted onto an inert support. The sample is treated in such a way that the DNA is liberated from microbes present in the sample and complexed onto the support. The DNA is normally denatured and fixed in this process. Subsequently, a labelled polynucleotide probe specific for a DNA sequence characteristic of a pathogenic product suspected of being present in the clinical sample is contacted with the fixed genomic single stranded nucleic acid under hybridizing conditions. Hybridization of probes to the single stranded nucleic acid is diagnostic of the presence of the pathogen.
4357421
SYNTHETIC GENE CODING FOR INFLUENZA HEMAGGLUTININ 3ohn S. Emtage, Norman Carey, High Wycombe, United Kingdom, assigned to G.D. Searle & Co A process for the production of a synthetic gene for an influenza haemagglutinin which comprises: (a) subjecting isolated vRNA to the action of reverse transcriptase to produce a double-stranded R N A / D N A hybrid; (b) digesting the RNA component of the hybrid; (c) treating the residual DNA component of the hybrid to produce a double-stranded DNA molecule having one hairpin end; and (d) treating the hairpin structure with a single-strand specific nuclease to produce a bimolecular double-stranded copy of the vRNA. 4357320 INFECTIOUS BRONCHITIS VACCINE FOR P O U L T R Y
144
PATENT ABSTRACTS
Peter Apontoweil, Manfred M. Krasselt, EK Leersum, Netherlands, assigned to GistBrocades N V Infectious bronchitis (IB) vaccines for poultry derived from at least one novel virus strain of novel infectious bronchitis serotypes, selected from the group consisting of culture Nos. CNCTC A 07/80, CNCTC A 08/80, CNCTC A 09/80, CNCTC A 010/80, CNCTC A 011,'80, CNCTC A 013/80, CNCTC A 014/80, CNCTC A 015/80 and CNCTC A 016/80 deposited at the Czechoslovak National Collection of Type Cultures of the Institute of Hygiene and Epidemiology in Prague, Czechoslovakia and the novel viruses per se, combined virus vaccaines and a novel method of protecting poultry from infectious bronchitis. 4357272 RECOVERING PURIFIED ANTIBODIES FROM EGG YOLK
Alfred Poison, Cape Town, South Africa, assigned to The South African Inventions Development Corporation
Immunological preparations are prepared by immunizing hens with an antigen, preferably to a stage of hyperimmunization. The eggs of the immunized hens are collected, the yolk is separated from the eggs, followed by separation of the lipid content of the yolk. The antibodies in the egg yolk are then rendered indispersable with the aid of a water-soluble linear filamentary non-charged polymer precipitant such as PEG and the indispersable antibodies are recovered. This precipitation of antibodies is preferably preceded by a precipitation of caseinaceous proteins at lower polymer concentrations. The immunological preparations are useful for diagnostic purposes and in appropriate cases also for the treatment of pathological conditions.
PATENT ABSTRACTS
4358538
4357422
MYCOBACTERIUM FORTUITUM MUTANT
M E T H O D OF ENHANCING INTERFERON P R O D U C T I O N
Merle G. Wovcha. Kevin E. Brooks, assigned to The Upjohn Company
Donald Giard, Robert J. Fleischaker, assigned to Massachusetts Institute of Technology,
Mutant which is used in a novel microbiological process to selectively transform steroids having 17-alkyl side chains of from 2 to 10 carbon atoms, inclusive, to yield sole transformed product. AD is a valuable intermediate to make useful steroids.
4358535
A method for enhancing the production of interferon from normal human diploid fibroblast cells is disclosed. In this method, a distinct interferon-production phase is established in which the temperature is initially elevated for a brief period followed by a reduction in temperature for the balance of the interferon-production phase.
SPECIFIC DNA PROBES IN DIAGNOSTIC MICROBIOLOGY Stanley Falkow, Stephen L. Moseley, assigned to Board of Regents of the University of Washington Method and compositions for infectious disease diagnosis and epidemiology involving labeled nucleotide probes complementary to nucleic acid coding for a characteristic pathogen product. Clinical isolates are cultivated, expanding the number of microorganisms, the resulting colonies lysed, the genome normally denatured and then fixed. Alternatively, clinical samples (stool, sputum, pus, etc.) are spotted onto an inert support. The sample is treated in such a way that the DNA is liberated from microbes present in the sample and complexed onto the support. The DNA is normally denatured and fixed in this process. Subsequently, a labelled polynucleotide probe specific for a DNA sequence characteristic of a pathogenic product suspected of being present in the clinical sample is contacted with the fixed genomic single stranded nucleic acid under hybridizing conditions. Hybridization of probes to the single stranded nucleic acid is diagnostic of the presence of the pathogen.
4357421
SYNTHETIC GENE CODING FOR INFLUENZA HEMAGGLUTININ 3ohn S. Emtage, Norman Carey, High Wycombe, United Kingdom, assigned to G.D. Searle & Co A process for the production of a synthetic gene for an influenza haemagglutinin which comprises: (a) subjecting isolated vRNA to the action of reverse transcriptase to produce a double-stranded R N A / D N A hybrid; (b) digesting the RNA component of the hybrid; (c) treating the residual DNA component of the hybrid to produce a double-stranded DNA molecule having one hairpin end; and (d) treating the hairpin structure with a single-strand specific nuclease to produce a bimolecular double-stranded copy of the vRNA. 4357320 INFECTIOUS BRONCHITIS VACCINE FOR P O U L T R Y
144
PATENT ABSTRACTS
Peter Apontoweil, Manfred M. Krasselt, EK Leersum, Netherlands, assigned to GistBrocades N V Infectious bronchitis (IB) vaccines for poultry derived from at least one novel virus strain of novel infectious bronchitis serotypes, selected from the group consisting of culture Nos. CNCTC A 07/80, CNCTC A 08/80, CNCTC A 09/80, CNCTC A 010/80, CNCTC A 011,'80, CNCTC A 013/80, CNCTC A 014/80, CNCTC A 015/80 and CNCTC A 016/80 deposited at the Czechoslovak National Collection of Type Cultures of the Institute of Hygiene and Epidemiology in Prague, Czechoslovakia and the novel viruses per se, combined virus vaccaines and a novel method of protecting poultry from infectious bronchitis. 4357272 RECOVERING PURIFIED ANTIBODIES FROM EGG YOLK
Alfred Poison, Cape Town, South Africa, assigned to The South African Inventions Development Corporation
Immunological preparations are prepared by immunizing hens with an antigen, preferably to a stage of hyperimmunization. The eggs of the immunized hens are collected, the yolk is separated from the eggs, followed by separation of the lipid content of the yolk. The antibodies in the egg yolk are then rendered indispersable with the aid of a water-soluble linear filamentary non-charged polymer precipitant such as PEG and the indispersable antibodies are recovered. This precipitation of antibodies is preferably preceded by a precipitation of caseinaceous proteins at lower polymer concentrations. The immunological preparations are useful for diagnostic purposes and in appropriate cases also for the treatment of pathological conditions.