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4371625 Vectors for the insertion therein of foreign DNA fragments, according to any translation phase
PATENT ABSTRACTS
properties. Extrachromosomal elements are used which are subject to external modulation for production of a control element. The change in the amount of production of the control element allows for enhanced expression o f a gene producing poly (amino acid) product. The change in production of the control element allowing for enhanced gene expression of the product is accompanied by amplification of the product producing gene. 4371625 VECTORS F O R THE INSERTION THEREIN OF FOREIGN D N A F R A G M E N T S , A C C O R D I N G TO ANY TRANSLATION P H A S E Pierre Tiollais, Paris, France, assigned to lnstitut Pasteur; Inst Nat'l de la Sante et Recherch The invention pertains to a set of vectors (or of DNA fragments to be inserted in a vector) which distinguish from one another in that, taking into account one vector in which the number of pairs of bases between the reading initiation point of the vector and a point corresponding to the first pair of bases of a recognition site corresponding to a predetermined restriction enzyme, the two other vectors comprise between the corresponding points additional groups of pairs of bases comprising respectively two pairs of bases on the one hand and either one or four pairs of bases on the other hand, plus possibly whole numbers of triplets. On inserting a determined DNA fragment of which the expression is sought in bacteria in the three vectors, the reading of said DNA fragment will occur in phase as concerns one of the so modified vectors after transfection of bacteria therewith. 4371615 M E T H O D FOR STABILIZING THE C H A R A C T E R I S T I C S OF MICROO R G A N I S M CONTAINING A PLASMID
97
Kiyoshi Miwa, Haruo Momose, Matsudo, Japan, assigned to Ajinomoto Company Incorporated A streptomycin dependent mutant of a microorganism of the genus escherichia which contains a plasmid containing genetic information controlling streptomycin independence maintains its properties when cultured in a medium devoid of streptomycin. The plasmid may also contain genetic information controlling the production of a chemical compound by the microorganism. Fermentation cultures of such microorganisms in media devoid of streptomycin do not lose their industrially desirable ability to synthesize useful compounds. 4366247
PROCESS F O R P R E P A R I N G TYLACTONE Richard H. Baltz, Eugene Send, assigned to Eli Lilly and Company O
CH3
CH31
~-CH2--CH3
~-~--~H~--~ ,4', o
2 ~'°"
oN,/
A process for preparing tylactone (20dihydro-20, 23-dideoxytylonolide), which has the formula: by submerged aerobic fermentation of Streptomyces fradiae NR RL 12188 or a tylactone-producing mutant or recombinant thereof is provided. ~66~6
M E T H O D FOR MICROBIAL POLYPEPTIDE EXPRESSION
properties. Extrachromosomal elements are used which are subject to external modulation for production of a control element. The change in the amount of production of the control element allows for enhanced expression o f a gene producing poly (amino acid) product. The change in production of the control element allowing for enhanced gene expression of the product is accompanied by amplification of the product producing gene. 4371625 VECTORS F O R THE INSERTION THEREIN OF FOREIGN D N A F R A G M E N T S , A C C O R D I N G TO ANY TRANSLATION P H A S E Pierre Tiollais, Paris, France, assigned to lnstitut Pasteur; Inst Nat'l de la Sante et Recherch The invention pertains to a set of vectors (or of DNA fragments to be inserted in a vector) which distinguish from one another in that, taking into account one vector in which the number of pairs of bases between the reading initiation point of the vector and a point corresponding to the first pair of bases of a recognition site corresponding to a predetermined restriction enzyme, the two other vectors comprise between the corresponding points additional groups of pairs of bases comprising respectively two pairs of bases on the one hand and either one or four pairs of bases on the other hand, plus possibly whole numbers of triplets. On inserting a determined DNA fragment of which the expression is sought in bacteria in the three vectors, the reading of said DNA fragment will occur in phase as concerns one of the so modified vectors after transfection of bacteria therewith. 4371615 M E T H O D FOR STABILIZING THE C H A R A C T E R I S T I C S OF MICROO R G A N I S M CONTAINING A PLASMID
97
Kiyoshi Miwa, Haruo Momose, Matsudo, Japan, assigned to Ajinomoto Company Incorporated A streptomycin dependent mutant of a microorganism of the genus escherichia which contains a plasmid containing genetic information controlling streptomycin independence maintains its properties when cultured in a medium devoid of streptomycin. The plasmid may also contain genetic information controlling the production of a chemical compound by the microorganism. Fermentation cultures of such microorganisms in media devoid of streptomycin do not lose their industrially desirable ability to synthesize useful compounds. 4366247
PROCESS F O R P R E P A R I N G TYLACTONE Richard H. Baltz, Eugene Send, assigned to Eli Lilly and Company O
CH3
CH31
~-CH2--CH3
~-~--~H~--~ ,4', o
2 ~'°"
oN,/
A process for preparing tylactone (20dihydro-20, 23-dideoxytylonolide), which has the formula: by submerged aerobic fermentation of Streptomyces fradiae NR RL 12188 or a tylactone-producing mutant or recombinant thereof is provided. ~66~6
M E T H O D FOR MICROBIAL POLYPEPTIDE EXPRESSION