4392933 Electrochemical measuring apparatus comprising enzyme electrode

PATENT ABSTRACTS

hydrolyzing triglycerides to'free glycerol in body fluids is disclosed. Substantially complete and rapid hydrolysis is provided by a mixture of enzymes using a ratio between 2:1 and 10:l, about 5:1 being preferred, the amounts representing units of activity of each lipase. Approximately 150 units of total lipases will provide substantially complete hydrolysis of the triglycerides contained in 20 microliters of serum up to a minimum concentration of 5 g./I. within 2.5 minutes at 37 degrees C.

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and for a period of time which is sufficient to substantially kill the contaminating microorganisms without significant deleterious effects on the immobilized enzymes. The substituted diethylenetriamine is preferably dioctyldicthylenetriamine or a mixture of dioctyldiethylenetriamine and trioctyldiethylenctrianline.

4393003 4393140 BETA-LACTAMASE PROCESS FOR THE PREPARATION OF THE HIGHLY PURE ENZYME KALLIKREIN FROM SWINE PANCREAS EXTRACTS Hermann Schutt, Wuppertal, Federal Republic Of Germany assigned to Bayer AktiengeselIschaft The invention relates to a process for the preparation of the highly pure enzyme kalliktein from organ extracts, in particular from swine pancreas extracts, the accompanying protcolytic activity of the swine pancreas extract being separated off by chromatography on ion exchangers and the highly pure enzyme kalliktein being obtained by chromatography on support materials with a kailiktein-trypsin inhibitor (basic pancreatic trypsin inhibitor = BPTI) covalently bonded in a particular manner.

4393138 METHOD FOR DISINFECTING IMMOBILIZED ENZYMES Jean-Lnc A G Batet, Motet, France assigned to Corning Glass Works Disinfecting of immobilized enzymes is carried out by contacting the immobilized enzymes with a dilute aqueous solution of at least one substituted diethylenetriamine at a concentration

INHIBITORS

Dennis Keith, John P Tengi, Manfre Weigele assigned to Hoffmann-La Roche Inc A beta-lactamase inhibitor of the formula See PatentJbr Chemical Structure I and the pharmaceutically acceptable salts thereof are presented. Also presented are intermediates and synthetic processes for the manufacture of the formula ! compound. The compound inhibits the activity of enzymes which inactivate certain betalactam antibiotics.

4392933 ELECTROCHEMICAL MEASURING APPARATUS COMPRISING ENZYME ELECTRODE Kenichi Nakamura, Shiro Nankai, Takashi lijima, Hirakata, Japan assigned to Matsushita Electric Industrial Co Ltd An immobilized enzyme electrode effective in measurement of the substrate concentration of the enzyme and in conversion from enzyme reaction energies into electric energies. The immobilized enzyme, of an oxidase system, such as glucose oxidase, amino acid oxidase, xanthine oxidase or the like and a metal oxide capable of constituting a redox system which is reduced through coupling with these enzyme reactions

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PATENT ABSTRACTS

and is electrochemically oxidized (anodic oxidation) are combined with each other. The use of the enzyme electrode allows the determination quantity of the enzyme substrate as extremely low as approximately 10-5 to 10-6 mole/l in concentration.

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4390628

PROCESSES FOR PRODUCING THERMOPHILIC ASPARTASE Kazuo Kimura, Kenichiro Takayama, Yutaka Ado, Tamotsu Kawamoto, Izumi Masunaga, Hofu, Japan assigned to Kyowa Hakko Kogyo Co Ltd Thermophilic aspartase is produced by culturing a microorganism belonging to the genus Bacillus. The enzyme is useful as a catalyst in the production of L-aspartic acid from ammonium fumarate or a mixture of fumaric acid and ammonia.

4391649 PROCESS FOR REGENERATING A STRONGLY ACIDIC CATION EXCHANGE RESIN Hiroshi Shimizu, Sigeo Sakai, Fumihik Matsoda, Reiko Matsumoto, Tokyo, Japan assigned to Japan Organo Co Ltd

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This invention relates to a process for regenerating a strongly acidic cation exchange resin which has used for a desalting treatment or partition chromatographic separation of a sugar liquid which lowers its separation capability, by means of contacting said strongly acidic cation exchange resin with an enzyme solution.

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PROCESS FOR ISOLATING CU, ZN-SUPEROXIDE DISMUTASE FROM AQUEOUS SOLUTIONS CONTAINING SAID ENZYME TOGETHER WITH ACCOMPANYING PROTEINS Jack Johansen, Rungsted Kyst, Denmark assigned to De Forenede Bryggerier A/S Cu,Zn-superoxide dismutase (SOD) is isolated from aqueous solutions containing said enzyme together with accompanying proteins by chromatography of the solution at a pH of 4.7 to 5.0 on a cation exchange resin of the same polarity as SOD in the pH range used. As cation exchange resin may particularly be used carboxymethyl celluloses, cross-linked dextrans substituted with carboxymethyl groups or sulfopropyl groups or cross-linked agaroses substituted with carboxymethyl groups. The process lends itself to use on an industrial scale and prorides a high yield of pure SOD.

4390622 NEISSERIA BACTERIA SPECIES IDENTIFICATION AND BETA LACTAMASE TESTING METHODS Garry W Cartwright

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Method for rapid, high intensity visual indication of the presence of Neisseria bacteria species utilizing a new and improved peptone buffer and pH indicator reagent.system as the specimen carricr for oxidation series testing, which series test