- Email: [email protected]
44 MICRORNAS DETECTION IN URINE SAMPLES FROM PATIENTS WITH UROTHELIAL CARCINOMA
42
THE EFFECTS OF URINARION FREQUENCY, WATER INTAKE AND SMOKING STATUS ON BLADDER CANCER RISK
*DUFLD5RMR '6LOYHUPDQ '*HODEHUW0DV $6DODGLH -05RWKPDQ 1 5HDO)*DUFLD&ORVDV00DODWV16HUUD&&DQWRU..RJHYLQDV0 7DUGRQ$'RVHPHFL0&DUUDWR$*DUFLD&ORVDV5 %LHOVD2 &HVSHGHV00DOHW-*DUFLD/RSH])7D³R-5RGU¯JXH]'H9HUD- *XDWH-/)HUQDQGH]-0$EDVFDO55LYDV0 'LD]00DWHRV$ 0XQWD³ROD3+XHUJR)0RVTXHUD-3UDWV- 1 Corporacio Parc Tauli, Dept. of Urology, Sabadell, Spain, 2National Cancer Institute, Dept. of Health and Human Services, Bethesda, United States of America, 3University Autonoma Barcelona, Dept. of Urology, Barcelona, Spain, 4 Hospital Germans Trias I Pujol, Dept. of Urology, Barcelona, Spain, 5IMIM, Dept. of Epidemiology, Barcelona, Spain, 6Corporacio Parc Tauli, Dept. of Epidemiology, Sabadell, Spain, 7University de Oviedo, Dept. of Medicine, Oviedo, Spain, 8Hospital General Elche, Dept. of Pathology, Elche, Spain, 9Hospital University de Canarias, Dept. of Epidemiology, La Laguna, Spain, 10Hospital del Mar, Dept. of Urology, Barcelona, Spain, 11Hospital Sant Boi de Llobregat, Dept. of Urology, Sant Boi de Llobregat, Spain, 12Hospital Manresa, Dept. of Urology, Manresa, Spain, 13Hospital General Elche, Dept. of Urology, Elche, Spain, 14Hospital la Candelaria, Dept. of Urology, Santa Cruz de Tenerife, Spain, 15 Hospital Universitario, Dept. of Urology, La Laguna, Spain, 16Hospital San Agustín, Dept. of Urology, Avilés, Spain, 17Hospital Central Covadonga, Dept. of Urology, Oviedo, Spain, 18Hospital Central General, Dept. of Urology, Oviedo, Spain, 19Hospital Cabueñes, Dept. of Urology, Gijón, Spain, 20Hospital de Jove, Dept. of Urology, Gijón, Spain, 21Hospital Cruz Roja, Dept. of Urology, Gijón, Spain, 22Hospital Alvarez-Buylla, Dept. of Urology, Mieres, Spain, 23Hospital Jario, Dept. of Urology, Coaña, Spain, 24Hospital Carmen y Severo Ochoa, Dept. of Urology, Cangas, Spain Introduction & Objectives: ([SHULPHQWDOVWXGLHVVXJJHVWWKDWLQFUHDVHGXULQDWLRQ IUHTXHQF\PD\UHGXFHEODGGHUFDQFHUULVNLIFDUFLQRJHQVDUHSUHVHQWLQWKHXULQH 2QO\ VPDOO VWXGLHV RI WKH HᚎHFW RI LQFUHDVHG XULQDWLRQ IUHTXHQF\ RQ EODGGHU FDQFHUULVNLQKXPDQVKDYHEHHQFRQGXFWHGZLWKFRQᚐLFWLQJUHVXOWV2XUSXUSRVH ZDV WR HYDOXDWH WKH HᚎHFW RI XULQDWLRQ IUHTXHQF\ RQ ULVN RI EODGGHU FDQFHU LQ D ODUJHPXOWLFHQWHUFDVHFRQWUROVWXG\ Material & Methods: :H DQDO\]HG GDWD EDVHG RQ LQWHUYLHZV FRQGXFWHG ZLWK SDWLHQWV ZLWK QHZO\ GLDJQRVHG EODGGHU FDQFHU DQG FRQWUROV IURP WRLQ6SDLQ:HHYDOXDWHGWKHHᚎHFWRIXULQDU\IUHTXHQF\ZDWHULQWDNHDQG VPRNLQJVWDWXVRQULVNRIEODGGHUFDQFHU0HQZLWKEHQLJQSURVWDWLFK\SHUWURSK\ ZHUH H[FOXGHG IURP DQDO\VLV :H REWDLQHG LQIRUPDWLRQ RQ DYHUDJH DGXOW ᚐXLG FRQVXPSWLRQVPRNLQJKDELWVGLHWDU\IDFWRUVRFFXSDWLRQDODQGUHVLGHQWLDOKLVWRULHV DQGKLVWRU\RIPHGLFDWLRQXVH7KHHᚎHFWVRIXULQDU\IUHTXHQF\RQEODGGHUFDQFHU ULVNZHUHTXDQWLᚏHGE\WKHRGGVUDWLR 25 25DQG FRQᚏGHQFHLQWHUYDOV &, ZHUHHVWLPHGE\XQFRQGLWLRQDOORJLVWLFUHJUHVVLRQ Results: :HREVHUYHGDFRQVLVWHQWLQYHUVHWUHQGLQULVNZLWKLQFUHDVLQJQLJKWWLPH YRLGLQJ IUHTXHQF\ LQ ERWK PHQ S DQG ZRPHQ S YRLGLQJ DW OHDVWWLPHVSHUQLJKWZDVDVVRFLDWHGZLWKDVLJQLᚏFDQWULVNUHGXFWLRQ 7KHSURWHFWLYHHᚎHFWRIQRFWXULDZDVDSSDUHQWDPRQJVWXG\SDUWLFLSDQWVZLWKORZ PRGHUDWHDQGKLJKZDWHUFRQVXPSWLRQ6XEMHFWVZKRYRLGHGDWOHDVWWZLFHSHUQLJKW DQGGUDQNDWOHDVWPOSHUGD\H[SHULHQFHGDVLJQLᚏFDQWUHGXFHGULVNRI EODGGHUFDQFHUFRPSDUHGWRWKRVHZKRGLGQRWYRLGDWQLJKWDQGGUDQNOHVVWKDQ PO RI ZDWHU SHU GD\ S 7KH ULVN DVVRFLDWHG ZLWK FLJDUHWWH VPRNLQJ ZDVUHGXFHGE\QRFWXULD&RPSDUHGZLWKQRQVPRNHUVZKRGLGQRWXULQDWHDWQLJKW FXUUHQWVPRNHUVZKRGLGQRWXULQDWHDWQLJKWKDGDQ25RI &, ZKHUHDVWKRVHZKRYRLGHGDWOHDVWWZLFHSHUQLJKWKDGDQ25RI &, S Conclusions: 2XUᚏQGLQJVVXJJHVWDVWURQJSURWHFWLYHHᚎHFWRIQRFWXULDRQEODGGHU FDQFHU ULVN SURYLGLQJ HYLGHQFH LQ KXPDQV WKDW EODGGHU FDQFHU ULVN LV UHODWHG WR WKHFRQWDFWWLPHRIWKHXURWKHOLXPZLWKFDUFLQRJHQVLQXULQH,QFUHDVHGXULQDWLRQ IUHTXHQF\FRXSOHGZLWKSRVVLEOHGLOXWLRQRIWKHXULQHIURPLQFUHDVHGZDWHULQWDNH PD\ GLPLQLVK WKH HᚎHFW RI XULQDU\ FDUFLQRJHQV RQ EODGGHU FDQFHU ULVN 6SRQVRU ,QWUDPXUDO5HVHDUFK3URJUDPRIWKH1,+1DWLRQDO&DQFHU,QVWLWXWH 86 DQG),6 6SDLQ
43
VALUE OF CLINICAL AND HISTOPATHOLOGICAL PARAMETERS FOR PROGNOSIS OF PT1 UROTHELIAL BLADDER CANCER: A SINGLE-CENTER ANALYSIS OF 309 PATIENTS
2WWR:)ULWVFKH+0%HUW]6%XUJHU05¸¡OHU::LHODQG:) +DUWPDQQ$'HQ]LQJHU6 1 University of Regensburg, Dept. of Urology, Regensburg, Germany, 2University of Erlangen, Dept. of Pathology, Erlangen, Germany Introduction & Objectives: ,W LV ZHOO NQRZQ WKDW S7 XURWKHOLDO FDQFHU RI WKH EODGGHULVDWXPRXUHQWLW\ZLWKGLYHUVHFOLQLFDOFRXUVHV6KDKLQHWDOVXPPDUL]HG ᚏQGLQJVRQSURJQRVLVRIS7EODGGHUFDQFHUZLWKWKHLUಱUXOHRIVಯPHDQLQJRQH WKLUGRISDWLHQWVHLWKHUGRQHYHUUHFXUGHYHORSPXVFOHLQYDVLYHWXPRXUVRUGLHRI PHWDVWDWLFGLVHDVH Material & Methods: :H UHWURVSHFWLYHO\ DQDO\VHG FOLQLFDO JHQGHU DQG
KLVWRSDWKRORJLFDO DUFKLWHFWXUH VL]H RI WXPRU IRFDOLW\ FDUFLQRPD LQ VLWX JUDGLQJ DFFRUGLQJ WR :+2 JUDGLQJ SDUDPHWHUV RI SDWLHQWV RI D VLQJOHFHQWUH WUHDWHGIRUSULPDU\S7EODGGHUFDQFHUEHWZHHQ DQG$OOFDVHVZHUH UHYLHZHG E\ WZR XURSDWKRORJLVWV 3DWLHQWV XQGHUZHQW WUDQVXUHWKUDO UHVHFWLRQ DQGJRWDGMXYDQWLQVWLOODWLRQVE\EDFLOOXV&DOPHWWH*X«ULQ %&* 3URJUHVVLRQWR PXVFOHLQYDVLYHGLVHDVHOHGWRF\VWHFWRP\LQIHZFDVHVHDUO\F\VWHFWRP\DWVWDJH S7ZDVSHUIRUPHG0HGLDQIROORZXSZDV PRQWKV UDQJHPRQWKV :H VWXGLHG \HDU UHFXUUHQFH IUHH SURJUHVVLRQ IUHH DQG WXPRXU VSHFLᚏFVXUYLYDO UDWHVLQUHODWLRQWRWKHPHQWLRQHGSDUDPHWHUV6WDWLVWLFDODQDO\VLVZDVSHUIRUPHG E\.DSODQ0HLHUDQDO\VLV Results: 2I SDWLHQWV ZHUH PDOH JUDGLQJ DFFRUGLQJ WR WKH :+2 FODVVLᚏFDWLRQ ZDV SRRUO\ GLᚎHUHQWLDWHG 7* LQ DQG PRGHUDWHO\ GLᚎHUHQWLDWHG * LQ 6ROLGWXPRXUJURZWKZLWKRXWSDSLOODU\FRPSRQHQWZDV IRXQG LQ RI SDWLHQWV ZKHUHDV KDG SDSLOODU\ WXPRXUV RI WXPRXUV PHDVXUHG!FPLQGLDPHWHUSDWLHQWV VKRZHGPXOWLIRFDOWXPRXUVDQG WKHVDPHIUHTXHQF\DVVRFLDWHGFDUFLQRPDLQVLWX &,6 2YHUDOOSDWLHQWV XQGHUZHQW F\VWHFWRP\ *HQGHU IRFDOLW\ DUFKLWHFWXUH DQG VL]H RI WXPRXU ZHUH QRW UHOHYDQW IRU SDWLHQWVC SURJQRVLV FRQFHUQLQJ UHFXUUHQFH DQG SURJUHVVLRQ DQG WXPRXUVSHFLᚏFVXUYLYDOZKLOHDVROLGWXPRXUJURZWKZDVDVVRFLDWHGZLWKDSRRUHU \HDUWXPRXUVSHFLᚏFVXUYLYDOWKDQSDSLOODU\DUFKLWHFWXUH YVS :KHUHDVSRRUJUDGLQJDQGFDUFLQRPDLQVLWXDOVRVKRZHGQRSURJQRVWLFYDOXHIRU UHFXUUHQFH WKHVH SDUDPHWHUV ZHUH VWDWLVWLFDOO\ VLJQLᚏFDQW DVVRFLDWHG ZLWK ORZHU \HDU WXPRXU VSHFLᚏF VXUYLYDO UDWHV 7* YV 7* S &,6 YVQR&,6S &,6ZDVDEOHWRSURJQRVWLFDWH\HDUSURJUHVVLRQ IUHHVXUYLYDOWRR &,6YVQR&,6S Conclusions: +LVWRSDWKRORJLFDO SDUDPHWHUV OLNH WXPRXU JUDGH DUFKLWHFWXUH DQG FDUFLQRPD LQ VLWX DUH UHODWHG WR WKH ULVN RI WXPRXU SURJUHVVLRQ DQG WXPRXU VSHFLᚏFVXUYLYDOLQS7EODGGHUFDQFHU&RPELQDWLRQZLWKLPPXQRKLVWRFKHPLFDO DQGPROHFXODUSDUDPHWHUVFRXOGLPSURYHGHWHUPLQDWLRQRISURJQRVLVDQGSRVVLEO\ WKHUDS\VWUDWLᚏFDWLRQLQS7EODGGHUFDQFHULQWKHIXWXUH
Poster Session 3 IMAGING, URINARY MARKERS AND MOLECULAR BIOLOGY OF UROTHELIAL CARCINOMA Saturday, 17 April, 14.15-15.45, Istanbul Room
44
MICRORNAS DETECTION IN URINE SAMPLES FROM PATIENTS WITH UROTHELIAL CARCINOMA
+LGHNL(&KL\RPDUX70DVDKLNR,.RMLPD61LVKL\DPD.1DRKLNR6 0DVD\XNL1 1 Kagoshima University, Dept. of Urology, Kagoshia, Japan, 2Teikyo University Chiba Medical Center, Dept. of Urology, Chiba, Japan, 3Chiba University, Dept. of Functional Genomics, Chiba, Japan Introduction & Objectives: &XUUHQWO\WXPRXUPDUNHUIRUXURWKHOLDOFDUFLQRPD 8& GHSHQGVRQXULQHF\WRORJ\EHFDXVHRILWVKLJKVHQVLWLYLW\ DURXQG GHVSLWHRI LWVORZVHQVLWLYLW\ DURXQG %ODGGHUWXPRXUDQWLJHQ %7$ DQGQXFOHDUPDWUL[ SURWHLQ 103 DUHDYDLODEOHDVXULQHPDUNHUVIRU8&GLDJQRVWLFWRROV+RZHYHU WKH\ DUH QRW ZLGHO\ XVHG EHFDXVH RI WKHLU ORZ VSHFLᚏFLW\ IRU GLVWLQJXLVKLQJ 8& IURP QRQPDOLJQDQW GLVHDVHV 0LFUR51$V DUH VPDOO QRQFRGLQJ 51$V RI DERXW QXFOHRWLGHVLQOHQJWKWKDWIXQFWLRQDVQHJDWLYHUHJXODWRUVRIJHQHH[SUHVVLRQ WKURXJK DQWLVHQVH FRPSOLPHQWDULO\ WR VSHFLᚏF PHVVHQJHU 51$V 5HFHQW VWXGLHV VXJJHVW WKDW DEHUUDQW PLFUR51$ H[SUHVVLRQ FRQWULEXWH WR WKH GHYHORSPHQW RI YDULRXVKXPDQPDOLJQDQFLHV2XUSUHYLRXVPL51$VFUHHQLQJRI8&GHPRQVWUDWHG WKDWPL5 DQGPL5ZHUHWKHWRSXSUHJXODWHGPL51$VDPRQJPL51$V H[DPLQHG :H K\SRWKHVL]H WKDW WKHVH PLFUR51$V DUH GHWHFWDEOH LQ XULQH DQG XVHIXOELRPDUNHUVIRUGLVWLQJXLVKLQJ8&IURPQRQ8&SDWLHQWV Material & Methods: 7RWDO51$RIXULQHZHUHH[WUDFWHGIURP8&VXULQDU\ WUDFW LQIHFWLRQV 87,V DQG KHDOWK\ FRQWUROV +&V $ UHDOWLPH 3&5 EDVHG H[SHULPHQWZDVXVHGWRHYDOXDWHH[SUHVVLRQOHYHOVRIWKHPL51$V7KHH[SUHVVLRQ OHYHO RI HDFK PL51$ ZDV FDOFXODWHG E\ D IROORZLQJ IRUPXOD H[SUHVVLRQ OHYHO A &W Results: 7KHH[SUHVVLRQOHYHOVRIPL5 DQGPL5LQXULQHIURP8&VZHUH VLJQLᚏFDQWO\ KLJKHU WKDQ WKDW IURP 87,V RU +&V HDFK 3 52& FXUYH DQDO\VLVUHYHDOHGWKDWPL5 DQGPL5KDYHJRRGVHQVLWLYLW\ DQG UHVSHFWLYHO\ DQGVSHFLᚏFLW\ DQGUHVSHFWLYHO\ WRGLVWLQJXLVK8&IURP QRQ8& SDWLHQW :H IRXQG VLJQLᚏFDQW GLᚎHUHQFH LQ PL5 H[SUHVVLRQV EHWZHHQ LQYDVLYH ุS7 DQGQRQLQYDVLYH S7D 8&V 3 EXWDWUHQGLQPL5 3 7KHUHZHUHVLJQLᚏFDQWGLᚎHUHQFHVLQPL5 RUPL5H[SUHVVLRQV EHWZHHQWKHVDPSOHVIURPSUHDQGSRVWRSHUDWLRQ 3 7RRXUVXUSULVHPL5 ZDVSRVLWLYHO\GHWHFWHGLQRI8&SDWLHQWVZKRKDGDQHJDWLYHGLDJQRVLVE\ XULQHF\WRORJ\:KHQZHFRPELQHGPLFUR51$DQDO\VLVZLWKXULQHF\WRORJ\LQRXU FRKRUWWKHVHQVLWLYLW\DQGWKHVSHFLᚏFLW\UHDFKHGWRDQG UHVSHFWLYHO\ IRU8&GHWHFWLRQ Conclusions: 2XUGDWDVXJJHVWWKDWGHWHFWLRQRIPL5 DQGPL5PD\VHUYH DVSRWHQWLDOELRPDUNHUVIRU8&'LDJQRVWLFDFFXUDF\IRUXURWKHOLDOFDUFLQRPDFDQ EHLPSURYHGE\DFRPELQDWLRQRIXULQHF\WRORJ\DQGWKHPLFUR51$VGHWHFWLRQ
Eur Urol Suppl 2010;9(2):47
THE EFFECTS OF URINARION FREQUENCY, WATER INTAKE AND SMOKING STATUS ON BLADDER CANCER RISK
*DUFLD5RMR '6LOYHUPDQ '*HODEHUW0DV $6DODGLH -05RWKPDQ 1 5HDO)*DUFLD&ORVDV00DODWV16HUUD&&DQWRU..RJHYLQDV0 7DUGRQ$'RVHPHFL0&DUUDWR$*DUFLD&ORVDV5 %LHOVD2 &HVSHGHV00DOHW-*DUFLD/RSH])7D³R-5RGU¯JXH]'H9HUD- *XDWH-/)HUQDQGH]-0$EDVFDO55LYDV0 'LD]00DWHRV$ 0XQWD³ROD3+XHUJR)0RVTXHUD-3UDWV- 1 Corporacio Parc Tauli, Dept. of Urology, Sabadell, Spain, 2National Cancer Institute, Dept. of Health and Human Services, Bethesda, United States of America, 3University Autonoma Barcelona, Dept. of Urology, Barcelona, Spain, 4 Hospital Germans Trias I Pujol, Dept. of Urology, Barcelona, Spain, 5IMIM, Dept. of Epidemiology, Barcelona, Spain, 6Corporacio Parc Tauli, Dept. of Epidemiology, Sabadell, Spain, 7University de Oviedo, Dept. of Medicine, Oviedo, Spain, 8Hospital General Elche, Dept. of Pathology, Elche, Spain, 9Hospital University de Canarias, Dept. of Epidemiology, La Laguna, Spain, 10Hospital del Mar, Dept. of Urology, Barcelona, Spain, 11Hospital Sant Boi de Llobregat, Dept. of Urology, Sant Boi de Llobregat, Spain, 12Hospital Manresa, Dept. of Urology, Manresa, Spain, 13Hospital General Elche, Dept. of Urology, Elche, Spain, 14Hospital la Candelaria, Dept. of Urology, Santa Cruz de Tenerife, Spain, 15 Hospital Universitario, Dept. of Urology, La Laguna, Spain, 16Hospital San Agustín, Dept. of Urology, Avilés, Spain, 17Hospital Central Covadonga, Dept. of Urology, Oviedo, Spain, 18Hospital Central General, Dept. of Urology, Oviedo, Spain, 19Hospital Cabueñes, Dept. of Urology, Gijón, Spain, 20Hospital de Jove, Dept. of Urology, Gijón, Spain, 21Hospital Cruz Roja, Dept. of Urology, Gijón, Spain, 22Hospital Alvarez-Buylla, Dept. of Urology, Mieres, Spain, 23Hospital Jario, Dept. of Urology, Coaña, Spain, 24Hospital Carmen y Severo Ochoa, Dept. of Urology, Cangas, Spain Introduction & Objectives: ([SHULPHQWDOVWXGLHVVXJJHVWWKDWLQFUHDVHGXULQDWLRQ IUHTXHQF\PD\UHGXFHEODGGHUFDQFHUULVNLIFDUFLQRJHQVDUHSUHVHQWLQWKHXULQH 2QO\ VPDOO VWXGLHV RI WKH HᚎHFW RI LQFUHDVHG XULQDWLRQ IUHTXHQF\ RQ EODGGHU FDQFHUULVNLQKXPDQVKDYHEHHQFRQGXFWHGZLWKFRQᚐLFWLQJUHVXOWV2XUSXUSRVH ZDV WR HYDOXDWH WKH HᚎHFW RI XULQDWLRQ IUHTXHQF\ RQ ULVN RI EODGGHU FDQFHU LQ D ODUJHPXOWLFHQWHUFDVHFRQWUROVWXG\ Material & Methods: :H DQDO\]HG GDWD EDVHG RQ LQWHUYLHZV FRQGXFWHG ZLWK SDWLHQWV ZLWK QHZO\ GLDJQRVHG EODGGHU FDQFHU DQG FRQWUROV IURP WRLQ6SDLQ:HHYDOXDWHGWKHHᚎHFWRIXULQDU\IUHTXHQF\ZDWHULQWDNHDQG VPRNLQJVWDWXVRQULVNRIEODGGHUFDQFHU0HQZLWKEHQLJQSURVWDWLFK\SHUWURSK\ ZHUH H[FOXGHG IURP DQDO\VLV :H REWDLQHG LQIRUPDWLRQ RQ DYHUDJH DGXOW ᚐXLG FRQVXPSWLRQVPRNLQJKDELWVGLHWDU\IDFWRUVRFFXSDWLRQDODQGUHVLGHQWLDOKLVWRULHV DQGKLVWRU\RIPHGLFDWLRQXVH7KHHᚎHFWVRIXULQDU\IUHTXHQF\RQEODGGHUFDQFHU ULVNZHUHTXDQWLᚏHGE\WKHRGGVUDWLR 25 25DQG FRQᚏGHQFHLQWHUYDOV &, ZHUHHVWLPHGE\XQFRQGLWLRQDOORJLVWLFUHJUHVVLRQ Results: :HREVHUYHGDFRQVLVWHQWLQYHUVHWUHQGLQULVNZLWKLQFUHDVLQJQLJKWWLPH YRLGLQJ IUHTXHQF\ LQ ERWK PHQ S DQG ZRPHQ S YRLGLQJ DW OHDVWWLPHVSHUQLJKWZDVDVVRFLDWHGZLWKDVLJQLᚏFDQWULVNUHGXFWLRQ 7KHSURWHFWLYHHᚎHFWRIQRFWXULDZDVDSSDUHQWDPRQJVWXG\SDUWLFLSDQWVZLWKORZ PRGHUDWHDQGKLJKZDWHUFRQVXPSWLRQ6XEMHFWVZKRYRLGHGDWOHDVWWZLFHSHUQLJKW DQGGUDQNDWOHDVWPOSHUGD\H[SHULHQFHGDVLJQLᚏFDQWUHGXFHGULVNRI EODGGHUFDQFHUFRPSDUHGWRWKRVHZKRGLGQRWYRLGDWQLJKWDQGGUDQNOHVVWKDQ PO RI ZDWHU SHU GD\ S 7KH ULVN DVVRFLDWHG ZLWK FLJDUHWWH VPRNLQJ ZDVUHGXFHGE\QRFWXULD&RPSDUHGZLWKQRQVPRNHUVZKRGLGQRWXULQDWHDWQLJKW FXUUHQWVPRNHUVZKRGLGQRWXULQDWHDWQLJKWKDGDQ25RI &, ZKHUHDVWKRVHZKRYRLGHGDWOHDVWWZLFHSHUQLJKWKDGDQ25RI &, S Conclusions: 2XUᚏQGLQJVVXJJHVWDVWURQJSURWHFWLYHHᚎHFWRIQRFWXULDRQEODGGHU FDQFHU ULVN SURYLGLQJ HYLGHQFH LQ KXPDQV WKDW EODGGHU FDQFHU ULVN LV UHODWHG WR WKHFRQWDFWWLPHRIWKHXURWKHOLXPZLWKFDUFLQRJHQVLQXULQH,QFUHDVHGXULQDWLRQ IUHTXHQF\FRXSOHGZLWKSRVVLEOHGLOXWLRQRIWKHXULQHIURPLQFUHDVHGZDWHULQWDNH PD\ GLPLQLVK WKH HᚎHFW RI XULQDU\ FDUFLQRJHQV RQ EODGGHU FDQFHU ULVN 6SRQVRU ,QWUDPXUDO5HVHDUFK3URJUDPRIWKH1,+1DWLRQDO&DQFHU,QVWLWXWH 86 DQG),6 6SDLQ
43
VALUE OF CLINICAL AND HISTOPATHOLOGICAL PARAMETERS FOR PROGNOSIS OF PT1 UROTHELIAL BLADDER CANCER: A SINGLE-CENTER ANALYSIS OF 309 PATIENTS
2WWR:)ULWVFKH+0%HUW]6%XUJHU05¸¡OHU::LHODQG:) +DUWPDQQ$'HQ]LQJHU6 1 University of Regensburg, Dept. of Urology, Regensburg, Germany, 2University of Erlangen, Dept. of Pathology, Erlangen, Germany Introduction & Objectives: ,W LV ZHOO NQRZQ WKDW S7 XURWKHOLDO FDQFHU RI WKH EODGGHULVDWXPRXUHQWLW\ZLWKGLYHUVHFOLQLFDOFRXUVHV6KDKLQHWDOVXPPDUL]HG ᚏQGLQJVRQSURJQRVLVRIS7EODGGHUFDQFHUZLWKWKHLUಱUXOHRIVಯPHDQLQJRQH WKLUGRISDWLHQWVHLWKHUGRQHYHUUHFXUGHYHORSPXVFOHLQYDVLYHWXPRXUVRUGLHRI PHWDVWDWLFGLVHDVH Material & Methods: :H UHWURVSHFWLYHO\ DQDO\VHG FOLQLFDO JHQGHU DQG
KLVWRSDWKRORJLFDO DUFKLWHFWXUH VL]H RI WXPRU IRFDOLW\ FDUFLQRPD LQ VLWX JUDGLQJ DFFRUGLQJ WR :+2 JUDGLQJ SDUDPHWHUV RI SDWLHQWV RI D VLQJOHFHQWUH WUHDWHGIRUSULPDU\S7EODGGHUFDQFHUEHWZHHQ DQG$OOFDVHVZHUH UHYLHZHG E\ WZR XURSDWKRORJLVWV 3DWLHQWV XQGHUZHQW WUDQVXUHWKUDO UHVHFWLRQ DQGJRWDGMXYDQWLQVWLOODWLRQVE\EDFLOOXV&DOPHWWH*X«ULQ %&* 3URJUHVVLRQWR PXVFOHLQYDVLYHGLVHDVHOHGWRF\VWHFWRP\LQIHZFDVHVHDUO\F\VWHFWRP\DWVWDJH S7ZDVSHUIRUPHG0HGLDQIROORZXSZDV PRQWKV UDQJHPRQWKV :H VWXGLHG \HDU UHFXUUHQFH IUHH SURJUHVVLRQ IUHH DQG WXPRXU VSHFLᚏFVXUYLYDO UDWHVLQUHODWLRQWRWKHPHQWLRQHGSDUDPHWHUV6WDWLVWLFDODQDO\VLVZDVSHUIRUPHG E\.DSODQ0HLHUDQDO\VLV Results: 2I SDWLHQWV ZHUH PDOH JUDGLQJ DFFRUGLQJ WR WKH :+2 FODVVLᚏFDWLRQ ZDV SRRUO\ GLᚎHUHQWLDWHG 7* LQ DQG PRGHUDWHO\ GLᚎHUHQWLDWHG * LQ 6ROLGWXPRXUJURZWKZLWKRXWSDSLOODU\FRPSRQHQWZDV IRXQG LQ RI SDWLHQWV ZKHUHDV KDG SDSLOODU\ WXPRXUV RI WXPRXUV PHDVXUHG!FPLQGLDPHWHUSDWLHQWV VKRZHGPXOWLIRFDOWXPRXUVDQG WKHVDPHIUHTXHQF\DVVRFLDWHGFDUFLQRPDLQVLWX &,6 2YHUDOOSDWLHQWV XQGHUZHQW F\VWHFWRP\ *HQGHU IRFDOLW\ DUFKLWHFWXUH DQG VL]H RI WXPRXU ZHUH QRW UHOHYDQW IRU SDWLHQWVC SURJQRVLV FRQFHUQLQJ UHFXUUHQFH DQG SURJUHVVLRQ DQG WXPRXUVSHFLᚏFVXUYLYDOZKLOHDVROLGWXPRXUJURZWKZDVDVVRFLDWHGZLWKDSRRUHU \HDUWXPRXUVSHFLᚏFVXUYLYDOWKDQSDSLOODU\DUFKLWHFWXUH YVS :KHUHDVSRRUJUDGLQJDQGFDUFLQRPDLQVLWXDOVRVKRZHGQRSURJQRVWLFYDOXHIRU UHFXUUHQFH WKHVH SDUDPHWHUV ZHUH VWDWLVWLFDOO\ VLJQLᚏFDQW DVVRFLDWHG ZLWK ORZHU \HDU WXPRXU VSHFLᚏF VXUYLYDO UDWHV 7* YV 7* S &,6 YVQR&,6S &,6ZDVDEOHWRSURJQRVWLFDWH\HDUSURJUHVVLRQ IUHHVXUYLYDOWRR &,6YVQR&,6S Conclusions: +LVWRSDWKRORJLFDO SDUDPHWHUV OLNH WXPRXU JUDGH DUFKLWHFWXUH DQG FDUFLQRPD LQ VLWX DUH UHODWHG WR WKH ULVN RI WXPRXU SURJUHVVLRQ DQG WXPRXU VSHFLᚏFVXUYLYDOLQS7EODGGHUFDQFHU&RPELQDWLRQZLWKLPPXQRKLVWRFKHPLFDO DQGPROHFXODUSDUDPHWHUVFRXOGLPSURYHGHWHUPLQDWLRQRISURJQRVLVDQGSRVVLEO\ WKHUDS\VWUDWLᚏFDWLRQLQS7EODGGHUFDQFHULQWKHIXWXUH
Poster Session 3 IMAGING, URINARY MARKERS AND MOLECULAR BIOLOGY OF UROTHELIAL CARCINOMA Saturday, 17 April, 14.15-15.45, Istanbul Room
44
MICRORNAS DETECTION IN URINE SAMPLES FROM PATIENTS WITH UROTHELIAL CARCINOMA
+LGHNL(&KL\RPDUX70DVDKLNR,.RMLPD61LVKL\DPD.1DRKLNR6 0DVD\XNL1 1 Kagoshima University, Dept. of Urology, Kagoshia, Japan, 2Teikyo University Chiba Medical Center, Dept. of Urology, Chiba, Japan, 3Chiba University, Dept. of Functional Genomics, Chiba, Japan Introduction & Objectives: &XUUHQWO\WXPRXUPDUNHUIRUXURWKHOLDOFDUFLQRPD 8& GHSHQGVRQXULQHF\WRORJ\EHFDXVHRILWVKLJKVHQVLWLYLW\ DURXQG GHVSLWHRI LWVORZVHQVLWLYLW\ DURXQG %ODGGHUWXPRXUDQWLJHQ %7$ DQGQXFOHDUPDWUL[ SURWHLQ 103 DUHDYDLODEOHDVXULQHPDUNHUVIRU8&GLDJQRVWLFWRROV+RZHYHU WKH\ DUH QRW ZLGHO\ XVHG EHFDXVH RI WKHLU ORZ VSHFLᚏFLW\ IRU GLVWLQJXLVKLQJ 8& IURP QRQPDOLJQDQW GLVHDVHV 0LFUR51$V DUH VPDOO QRQFRGLQJ 51$V RI DERXW QXFOHRWLGHVLQOHQJWKWKDWIXQFWLRQDVQHJDWLYHUHJXODWRUVRIJHQHH[SUHVVLRQ WKURXJK DQWLVHQVH FRPSOLPHQWDULO\ WR VSHFLᚏF PHVVHQJHU 51$V 5HFHQW VWXGLHV VXJJHVW WKDW DEHUUDQW PLFUR51$ H[SUHVVLRQ FRQWULEXWH WR WKH GHYHORSPHQW RI YDULRXVKXPDQPDOLJQDQFLHV2XUSUHYLRXVPL51$VFUHHQLQJRI8&GHPRQVWUDWHG WKDWPL5 DQGPL5ZHUHWKHWRSXSUHJXODWHGPL51$VDPRQJPL51$V H[DPLQHG :H K\SRWKHVL]H WKDW WKHVH PLFUR51$V DUH GHWHFWDEOH LQ XULQH DQG XVHIXOELRPDUNHUVIRUGLVWLQJXLVKLQJ8&IURPQRQ8&SDWLHQWV Material & Methods: 7RWDO51$RIXULQHZHUHH[WUDFWHGIURP8&VXULQDU\ WUDFW LQIHFWLRQV 87,V DQG KHDOWK\ FRQWUROV +&V $ UHDOWLPH 3&5 EDVHG H[SHULPHQWZDVXVHGWRHYDOXDWHH[SUHVVLRQOHYHOVRIWKHPL51$V7KHH[SUHVVLRQ OHYHO RI HDFK PL51$ ZDV FDOFXODWHG E\ D IROORZLQJ IRUPXOD H[SUHVVLRQ OHYHO A &W Results: 7KHH[SUHVVLRQOHYHOVRIPL5 DQGPL5LQXULQHIURP8&VZHUH VLJQLᚏFDQWO\ KLJKHU WKDQ WKDW IURP 87,V RU +&V HDFK 3 52& FXUYH DQDO\VLVUHYHDOHGWKDWPL5 DQGPL5KDYHJRRGVHQVLWLYLW\ DQG UHVSHFWLYHO\ DQGVSHFLᚏFLW\ DQGUHVSHFWLYHO\ WRGLVWLQJXLVK8&IURP QRQ8& SDWLHQW :H IRXQG VLJQLᚏFDQW GLᚎHUHQFH LQ PL5 H[SUHVVLRQV EHWZHHQ LQYDVLYH ุS7 DQGQRQLQYDVLYH S7D 8&V 3 EXWDWUHQGLQPL5 3 7KHUHZHUHVLJQLᚏFDQWGLᚎHUHQFHVLQPL5 RUPL5H[SUHVVLRQV EHWZHHQWKHVDPSOHVIURPSUHDQGSRVWRSHUDWLRQ 3 7RRXUVXUSULVHPL5 ZDVSRVLWLYHO\GHWHFWHGLQRI8&SDWLHQWVZKRKDGDQHJDWLYHGLDJQRVLVE\ XULQHF\WRORJ\:KHQZHFRPELQHGPLFUR51$DQDO\VLVZLWKXULQHF\WRORJ\LQRXU FRKRUWWKHVHQVLWLYLW\DQGWKHVSHFLᚏFLW\UHDFKHGWRDQG UHVSHFWLYHO\ IRU8&GHWHFWLRQ Conclusions: 2XUGDWDVXJJHVWWKDWGHWHFWLRQRIPL5 DQGPL5PD\VHUYH DVSRWHQWLDOELRPDUNHUVIRU8&'LDJQRVWLFDFFXUDF\IRUXURWKHOLDOFDUFLQRPDFDQ EHLPSURYHGE\DFRPELQDWLRQRIXULQHF\WRORJ\DQGWKHPLFUR51$VGHWHFWLRQ
Eur Urol Suppl 2010;9(2):47