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4405711 Analysis element for immunochemical measurement of trace components and method for immunochemical measurement using the same
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PATENT ABSTRACTS
R I is See Patent ./or Chemical Structure R2 is CH2CH(CH3)2, (ii) hippuricase and (iii) 4aminoantipyrine, with a liquid containing an angiotensin-converting enzyme; measuring colorimetrically the concentration of the quinonimine dye which is formed by adding an oxiding agent to the above mixture; and calculating the activity of the angiotensinconverting enzyme from the concentration of the quinonimine dye.
4405711 ANALYSIS ELEMENT FOR IMMUNOCHEMICAL MEASUREMENT OF TRACE COMPONENTS AND METHOD FOR IMMUNOCHEMICAL MEASUREMENT USING THE SAME Nobuhito Masuda, Shigeru Nagatomo, Yuji Mihara, Minami ashigara, Japan assigned to Fuji Photo Film Co Ltd
4406832 PEPTIDE-TYPE SUBSTRATES U S E F U L IN T H E Q U A N T I T A T I V E DETERMINATION OF ENDOTOXIN Donald F Mills assigned to Mallinckrodt Inc
Disclosed are chromogenic or fluorogenic peptide-type compounds adapted for use in determining endotoxin in a sample by a Limulus amebocyte lysate-type assay. The compounds have the general formula: See Patent for Tabular Presentation PS wherein RI represents hydrogen, a blocking aromatic hydrocarbon or acyl; AI represents an L or D-amino acid selected from either lieu, Val or Leu; A2 represents Glu or Asp; A3 represents Ala or Cyst; A4 represents Arg, B represents a linkage group selected from ester and amide linkage groups; and R2 represents a chromogenic or fluorogenic group which is covalently attached to the C-carboxyl terminal ofarginine through the B linkage group and which yields a chromophoric or fluorescent marker compound of the formula R2-B-H upon enzymatic hydrolysis from the remainder of the peptide-type compound by activated LAL proclot enzyme. Also disclosed are methods for the determination of endotoxin in a sample by contacting the sample with a pro-clotting enzyme from Limulus amebocyte lysate and one of the above chromogenic or fluorogenic peptide-type compounds.
In a method of assay for a trace component such as antigen, antibody or enzyme utilizing immunochemical reactionor enzyme reaction in combination with photographic detection system comprising measuring optical density of a silver image formed in proportion to the antigen, antibody or enzyme to be measured, a novel analysis sheet comprising a support having provided thereon, in succession, a water absorbing layer and a silver halide emulsion layer is employed. The analysis sheet essentially increases the amount of water absorbed so that the analysis sheet is extremely, effective for improving detection sensitivity.
4405601 EXTRACTION AND PURIFICATION OF BIOLOGICALLY ACTIVE LYMPHOKINES John McEnfire, Ben Papermaster assigned to Cancer Research Center A biologically active lymphokine fraction which exhibits macrophag¢ activation activity and lyrnphotoxin activity is prepared from large scale supernatant culture fluids of human lymphoblastoid cultured cell lines grown in the presence of human serum to minimize antigenicity. Growth of iymphoblastoid cells from human lymphoblastoid cultured cell lines is effected and the supernatant culture fluids are harvested, concentrated and clarified. The supernatant culture fluids arc then extracted with trichloroacetie
PATENT ABSTRACTS
R I is See Patent ./or Chemical Structure R2 is CH2CH(CH3)2, (ii) hippuricase and (iii) 4aminoantipyrine, with a liquid containing an angiotensin-converting enzyme; measuring colorimetrically the concentration of the quinonimine dye which is formed by adding an oxiding agent to the above mixture; and calculating the activity of the angiotensinconverting enzyme from the concentration of the quinonimine dye.
4405711 ANALYSIS ELEMENT FOR IMMUNOCHEMICAL MEASUREMENT OF TRACE COMPONENTS AND METHOD FOR IMMUNOCHEMICAL MEASUREMENT USING THE SAME Nobuhito Masuda, Shigeru Nagatomo, Yuji Mihara, Minami ashigara, Japan assigned to Fuji Photo Film Co Ltd
4406832 PEPTIDE-TYPE SUBSTRATES U S E F U L IN T H E Q U A N T I T A T I V E DETERMINATION OF ENDOTOXIN Donald F Mills assigned to Mallinckrodt Inc
Disclosed are chromogenic or fluorogenic peptide-type compounds adapted for use in determining endotoxin in a sample by a Limulus amebocyte lysate-type assay. The compounds have the general formula: See Patent for Tabular Presentation PS wherein RI represents hydrogen, a blocking aromatic hydrocarbon or acyl; AI represents an L or D-amino acid selected from either lieu, Val or Leu; A2 represents Glu or Asp; A3 represents Ala or Cyst; A4 represents Arg, B represents a linkage group selected from ester and amide linkage groups; and R2 represents a chromogenic or fluorogenic group which is covalently attached to the C-carboxyl terminal ofarginine through the B linkage group and which yields a chromophoric or fluorescent marker compound of the formula R2-B-H upon enzymatic hydrolysis from the remainder of the peptide-type compound by activated LAL proclot enzyme. Also disclosed are methods for the determination of endotoxin in a sample by contacting the sample with a pro-clotting enzyme from Limulus amebocyte lysate and one of the above chromogenic or fluorogenic peptide-type compounds.
In a method of assay for a trace component such as antigen, antibody or enzyme utilizing immunochemical reactionor enzyme reaction in combination with photographic detection system comprising measuring optical density of a silver image formed in proportion to the antigen, antibody or enzyme to be measured, a novel analysis sheet comprising a support having provided thereon, in succession, a water absorbing layer and a silver halide emulsion layer is employed. The analysis sheet essentially increases the amount of water absorbed so that the analysis sheet is extremely, effective for improving detection sensitivity.
4405601 EXTRACTION AND PURIFICATION OF BIOLOGICALLY ACTIVE LYMPHOKINES John McEnfire, Ben Papermaster assigned to Cancer Research Center A biologically active lymphokine fraction which exhibits macrophag¢ activation activity and lyrnphotoxin activity is prepared from large scale supernatant culture fluids of human lymphoblastoid cultured cell lines grown in the presence of human serum to minimize antigenicity. Growth of iymphoblastoid cells from human lymphoblastoid cultured cell lines is effected and the supernatant culture fluids are harvested, concentrated and clarified. The supernatant culture fluids arc then extracted with trichloroacetie