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4409326 Stabilized enzymatic solutions and method for determining total cholesterol in human serum
PATENT ABSTRACTS at about I000 cm-I in the BuChE-Malathion spectrum. The enzyme is used to collect and concentrate the agent or pollutant, and the resulting complexed enzyme can then be analyzed (e.g.. by infrared spectroscopy) and its spectrum compared to an uninhibited enzyme spectrum. Relatively simple devices can carry out the collection and detection or monitoring or identification steps of this invention, given appropriate models of complexed and uncomplexed enzyme specta upon which to base the design of the devices.
~1~25
333
being clear and colorless; a solution of 50000 [ U of which in 3 ml. carbon dioxide-free physiological ,saline solution suitable for injection has a light absorption of not more than 0.60 at 280 nm and of not more than 0.37 at 260 rim; the enzyme having the following percentage amino acid a,mlysis: See Patent ./'or Tabular Presentation PS said enzyme having a total hexose content of 10.6+/-0.003°,,-~; said enzyme containing not more than 5 mug. albumin per 220000 IU of activity and said enzyme having a molecular weight of 62{)00 to 70000 determined by gel filtration. The present invention also provides a process for preparing this enzyme and a dosage unit containing it.
SALT-TOLERANT MICROBIAL XANTHANASE AND METHOD OF PRODUCING SAME
4409326
Martin C Cadmus assigned to The United States of America as represented by the Secretary of Agriculture
STABILIZED ENZYMATIC SOLUTIONS AND METHOD FOR DETERMINING TOTAL CHOLESTEROL IN HUMAN SERUM
A novel Bacillus isolated from the soil is unique in its capacity to produce xanthanase, and is especially productive of the enzyme when cultured in the presence o f other soil organisms. Both crude and purified xanthanases recovered from the fermentation broth effectively degrade xanthan gum. Moreover, tolerance of the Bacillus to sodium chloride levels as high as about 4~o, render it useful for the in situ degradation of the heteropolysaocharide in petroleum recovery fluids and other thickened industrial brines.
~1~31 ENZYME DERIVED FROM HYALURONIDASE Derek J Pope, Lily Baxendale, Milton Keynes, United Kingdom assigned to Biorex Laboratories Limited The present invention provides an enzyme which is an odorless, white powder, the pH of a 15000 to 22000 IU/ml. solution of which in carbon dioxide-free physiological saline solution suitable for injection is 4.5 to 7.5, the solution
Ivan E Modrovich
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Stabilized enzymes useful in the diagnostic assay of total cholesterol are prepared by combining three separately prepared reagents: (l) a cholesterol esterasc reagent; (2) cholesterol oxidase reagent: and (3) a saline diluent reagent. The cholesterol esterase reagent is prepared by dissolving a salt ofcholic acid in a buffer solution providing a pH within the range of about 4 to about 9. To this solution is added a cholesterol esterase. The solution is then mixed with a polyhydroxy organic compound and TRITON-X100. A cholesterol oxidase and a polyhydroxy organic compound are each dissolved in separate portions of buffer solution to make the cholesterol esterase reagent. The saline diluent
334
PATENT ABSTRACTS
solution is prepared by dissolving potassium iodide in water and adding a polyhydroxy organic compound. The resultant three reagents, when combined, provide a stabilized enzyme solution having utility in the total cholesterol assay of a serum sample. The combined reagents have utility in a total oxygen consumption method of analysis of total cholesterol, which analysis method is also disclosed.
4409140 SUBSTRATES AND METHOD FOR DETERMINING ENZYMES Robert Smith, Eugene R Bissell
CF3
4409325 VALPROATE CONJUGATION USING DICARBONYLS Damon K Leung, Prithipal Singh assigned to Syva Company
RtCO)m2W4[1 tCH)CH:CH2)2C Zt I COOH n2
i
r,co,.2w. COOH ,,Jml
alpha-substituted derivatives of valproic acid are provided for conjugation to antigenic compositions, particularly poly(amino acids), and enzymes. The antigenic conjugates are employed for the production of antibodies, which find particular use in immunoassays for the determination of valproate, while the enzyme conjugate finds use in a homogeneous enzyme immunoassay for the determination of valproate. The compounds are synthesized by alkylating valproate at the tertiary carbon atom by an aliphatic chain with a terminal double bond which is cleaved to provide an acid or aldehyde group.
A method is disclosed for determining the presence of an enzyme in a biological fuid, which includes the steps of contacting the fluid with a synthetic chromogenic substrate, which is an amino acid derivative of 7-amino-4trifluoromethylcoumarin; incubating the substrate-containing fluid to effect enzymatic hydrolysis; and fluorometrically determining the presence of the free 7-amino-4trifluoromethylcoumarin chromophore in the hydrolyzate.
4407975 POLYMERIC MEMBRANE HAVING MALEIC ANHYDRIDE RESIDUES Tomohik Yamaguchi, Yatake, Japan assigned to Agency of Industrial Science and Technology
A polymeric membrane having maleic anhydride residues which comprises a blend of a maleic anhydride copolymer and a support polymer, said blend being partially cross-linked with a bifunctional cross-linking reagent. When hydrolyzed, this membrane turns into an ion exchange membrane or a membrane on which an enzyme is immobilized.
~1~25
333
being clear and colorless; a solution of 50000 [ U of which in 3 ml. carbon dioxide-free physiological ,saline solution suitable for injection has a light absorption of not more than 0.60 at 280 nm and of not more than 0.37 at 260 rim; the enzyme having the following percentage amino acid a,mlysis: See Patent ./'or Tabular Presentation PS said enzyme having a total hexose content of 10.6+/-0.003°,,-~; said enzyme containing not more than 5 mug. albumin per 220000 IU of activity and said enzyme having a molecular weight of 62{)00 to 70000 determined by gel filtration. The present invention also provides a process for preparing this enzyme and a dosage unit containing it.
SALT-TOLERANT MICROBIAL XANTHANASE AND METHOD OF PRODUCING SAME
4409326
Martin C Cadmus assigned to The United States of America as represented by the Secretary of Agriculture
STABILIZED ENZYMATIC SOLUTIONS AND METHOD FOR DETERMINING TOTAL CHOLESTEROL IN HUMAN SERUM
A novel Bacillus isolated from the soil is unique in its capacity to produce xanthanase, and is especially productive of the enzyme when cultured in the presence o f other soil organisms. Both crude and purified xanthanases recovered from the fermentation broth effectively degrade xanthan gum. Moreover, tolerance of the Bacillus to sodium chloride levels as high as about 4~o, render it useful for the in situ degradation of the heteropolysaocharide in petroleum recovery fluids and other thickened industrial brines.
~1~31 ENZYME DERIVED FROM HYALURONIDASE Derek J Pope, Lily Baxendale, Milton Keynes, United Kingdom assigned to Biorex Laboratories Limited The present invention provides an enzyme which is an odorless, white powder, the pH of a 15000 to 22000 IU/ml. solution of which in carbon dioxide-free physiological saline solution suitable for injection is 4.5 to 7.5, the solution
Ivan E Modrovich
\ I~.2 ' \
,~,,,
Stabilized enzymes useful in the diagnostic assay of total cholesterol are prepared by combining three separately prepared reagents: (l) a cholesterol esterasc reagent; (2) cholesterol oxidase reagent: and (3) a saline diluent reagent. The cholesterol esterase reagent is prepared by dissolving a salt ofcholic acid in a buffer solution providing a pH within the range of about 4 to about 9. To this solution is added a cholesterol esterase. The solution is then mixed with a polyhydroxy organic compound and TRITON-X100. A cholesterol oxidase and a polyhydroxy organic compound are each dissolved in separate portions of buffer solution to make the cholesterol esterase reagent. The saline diluent
334
PATENT ABSTRACTS
solution is prepared by dissolving potassium iodide in water and adding a polyhydroxy organic compound. The resultant three reagents, when combined, provide a stabilized enzyme solution having utility in the total cholesterol assay of a serum sample. The combined reagents have utility in a total oxygen consumption method of analysis of total cholesterol, which analysis method is also disclosed.
4409140 SUBSTRATES AND METHOD FOR DETERMINING ENZYMES Robert Smith, Eugene R Bissell
CF3
4409325 VALPROATE CONJUGATION USING DICARBONYLS Damon K Leung, Prithipal Singh assigned to Syva Company
RtCO)m2W4[1 tCH)CH:CH2)2C Zt I COOH n2
i
r,co,.2w. COOH ,,Jml
alpha-substituted derivatives of valproic acid are provided for conjugation to antigenic compositions, particularly poly(amino acids), and enzymes. The antigenic conjugates are employed for the production of antibodies, which find particular use in immunoassays for the determination of valproate, while the enzyme conjugate finds use in a homogeneous enzyme immunoassay for the determination of valproate. The compounds are synthesized by alkylating valproate at the tertiary carbon atom by an aliphatic chain with a terminal double bond which is cleaved to provide an acid or aldehyde group.
A method is disclosed for determining the presence of an enzyme in a biological fuid, which includes the steps of contacting the fluid with a synthetic chromogenic substrate, which is an amino acid derivative of 7-amino-4trifluoromethylcoumarin; incubating the substrate-containing fluid to effect enzymatic hydrolysis; and fluorometrically determining the presence of the free 7-amino-4trifluoromethylcoumarin chromophore in the hydrolyzate.
4407975 POLYMERIC MEMBRANE HAVING MALEIC ANHYDRIDE RESIDUES Tomohik Yamaguchi, Yatake, Japan assigned to Agency of Industrial Science and Technology
A polymeric membrane having maleic anhydride residues which comprises a blend of a maleic anhydride copolymer and a support polymer, said blend being partially cross-linked with a bifunctional cross-linking reagent. When hydrolyzed, this membrane turns into an ion exchange membrane or a membrane on which an enzyme is immobilized.