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441. Significance of oestrogens in relation to prostaglandin F changes at parturition in the sheep
Abstracts 440. Steroid and prostaghmdin levels in ewes bearing uteroovarian autotransplants
SCARAMUZZI, R. J., BAIRD,D. T., LAND, R. B. and WHEELER,A. G., MRC Unit of Reproductive Biology, 39 Chalmers Street, Edinburgh, Scotland The concentrations of androstenedione, oestradiol, progesterone and prostaglandin F (PGF) were measured in utero-ovarian venous plasma of 4 ewes bearing uteroovarian transplants. (Harrison, Heap, Linzell (1968) J.Enhcr. 40 xiii). All animals had regular oestrus cycles with a mean + SEM of 17.6 + 0.2 days (n = 20). Throughout the oestrus cycle the concentrations of both oestradiol and androstenedione fluctuated and peaks of these hormones occurred randomly at 2-6 day intervals. The levels of oestradiol and androstenedione were highest 3 days prior to oestrus(65 f 10 and 320 + 114pg/mlrespectivel.y; n = 6). The levels of androstenedione and oestradiol were correlated indicating a common ovarian source probably the follicle (r = 0.62-0.96, P < 0~05-0~001n = 9-32). The concentration of PGF remained below 2OOpg/ml until Day 12, when it rose to a mean value of 384 k 104 (n = 3) pg/ml. This was followed by a much larger rise on the day before oestrus(5200 pg/ml n = 3). Progesterone levels started to decline on Day 13 and fell to less than 5 ng/ml on the day prior to oestrus. These studies make it unlikely that the pre-ovulatory secretion of oestradiol is responsible for the release of PGF which initiates luteal regression. 441. Significance of oestrogens in relation to prostaglandin F changes at parturition in the sheep Cox, R. I., WONG, M., CURRIE, W. B. and THOR-
BURN, G. D., C.S.I.R.O. Division of Animal Physiology, P.O. Box 239, Blacktown 2148, Australia The investigation was aimed at clarifying the role of oestrogen and prostaglandin changes in the initiation of parturition in the sheep. Blood samples were taken through cannulae chronically inserted in foetal carotid arteries or maternal utero-ovarian veins. Analyses were by competitive protein binding or radioimmunoassays. Measurement of maxima in E28> Ezw, El and their sulphates indicated concentrations at about 125 days of gestation as well as at parturition. Infusion of physiological amounts of E2fi into the ewe or of some potential oestrogen precursors into the foetus in late pregnancy led to an increase in PGF in the utero-ovarian vein and premature parturition. Premature parturition induced by corticosteroids infused into the foetus also resulted in both oestrogen and then PGF concentrations increasing. Analysis of foetal plasma for possible oestrogen precursors indicated that the classical Cl9 compounds were virtually absent. Some possible alternative intermediates were detected. The data are consistent with oestrogen from the conceptus stimulating uterine PGF biosynthesis or release, probably after transfer of the oestrogen to the maternal compartment. 442. Effect of prostaglandin F2a on gravid human uterine
401
three sub-groups! Molar-pregnancy (8 cases), fetal death (6 cases) and anencephaly (2 cases), subdividing these into two categories: Nuliparas and multiparas. Prostaglandin F2a was administered through amniotic puncture because this is one of the less explored routes to induce labor. A sample of patients with molar pregnancy, fetal death or anencephaly was chosen because the effect of prostaglandins on the product is unknown., Doses used (5 to 200 pg) were low compared to those given by others, since response determinesuterine reactivity. Prostaglandin was prepared in ethanol solution and there was no effect of this product on uterine contractivity. We feel it is better to start stimulation of uterine contractivity with low doses and increase them gradually according to response obtained, than starting with high doses. Dose-response curves were obtained showing the doses of prostaglandin versus the mean values of tonus, intensity, frequency and uterine activity. It was found that these four parameters increased when the dose of prostaglandin was augmented. In all three groups studied the uterine activity, cervical efacement and dilation were calculated. There was no significant changes in blood pressure readings during the first hours of the study, whereas the final part of the tracings showed an increase of differential due to elevation of the systolic component. Patients with fetal death had the cervical dilatiofi registered and the mean time required to dilate from 2 to IOcm was 9.50 h. Side effects observed were nausea, vomiting and chills. One patient had hypotension. Four patients had complications such as: Trophoblastic pulmonary embolism (one possible case), bleeding and hypotension and deciduo-myometritis. The remaining patients had no complications during the 2nd and 3rd phases of labor. Prostaglandin given by amniotic puncture caused few and mild side-effects. 443. Serum progesterone, human chorionic somatomammotropin, estriol and cortisol following intra- and extraamniotic administration of prostaglandin
RUNNEBAUM, B.. GERHARD,I., HENNER,H.. VEC’SEI. P., HALLER,U., R~~TTGERS, H. and KUBLI. F., Universitlts-Frauenklinik, Heidelberg, West Germany The present investigation was undertaken to evaluate changes in feto-placental and maternal hormone secretion following intra- and extra-amniotic injection of prostaglandin for induction of abortion. Before and after the administration of prostaglandin serial determinatiops of progesterone, human chorionic somatomammotropin, estriol and cortisol were performed by radioimmunoassay in the same serum samples during the induction-abortion-interval. In 13 pregnant women (week 14-20) induction of abortion was carried out by intra-amniotic installation of PGF!, and in 12 pregnant women (week 14-23) by intra-amniotic instillation of PGE,. In another group of pregnant women (week 11-16) PGF2, was applied by extra-amniotic injection for induction of abortion. The changes in the hormone levels during the inductionabortion interval are compared with the mode of uterine activity after intra- and extra-amniotic administration of the prostaglandins.
contractility
ROMERO-SALINAS, G., RUIZ-VELASCO,V., RAMIREZ- 444. Kinetic study of the action of gonadotropins and prostaghmdhm on oestrogen bioqkhesis, glycogenJIMENEZ,D., GARCIA-Pti~, J. and BRAVO-SANDOphosuhorvlase and cyclic AMP levels in human fullVAL, J., Department of Obstetric Physiology, Hospital ~erm~ph&ntas perfused “in vitro” de Gineco-Obstetricia No. 3, Centro Medico La Raza, ALSAT,E., LEVILLIEXS, J., AUGUY,A. and CEDARD,L., I.M.S.S., Mexico, D.F., Mexico City Facultt de MCdecine Cochin-Port-Royal (E.R. CNRS 123, U 35 INSERM) Paris, France A sample group of 16 patients was studied, divided into
SCARAMUZZI, R. J., BAIRD,D. T., LAND, R. B. and WHEELER,A. G., MRC Unit of Reproductive Biology, 39 Chalmers Street, Edinburgh, Scotland The concentrations of androstenedione, oestradiol, progesterone and prostaglandin F (PGF) were measured in utero-ovarian venous plasma of 4 ewes bearing uteroovarian transplants. (Harrison, Heap, Linzell (1968) J.Enhcr. 40 xiii). All animals had regular oestrus cycles with a mean + SEM of 17.6 + 0.2 days (n = 20). Throughout the oestrus cycle the concentrations of both oestradiol and androstenedione fluctuated and peaks of these hormones occurred randomly at 2-6 day intervals. The levels of oestradiol and androstenedione were highest 3 days prior to oestrus(65 f 10 and 320 + 114pg/mlrespectivel.y; n = 6). The levels of androstenedione and oestradiol were correlated indicating a common ovarian source probably the follicle (r = 0.62-0.96, P < 0~05-0~001n = 9-32). The concentration of PGF remained below 2OOpg/ml until Day 12, when it rose to a mean value of 384 k 104 (n = 3) pg/ml. This was followed by a much larger rise on the day before oestrus(5200 pg/ml n = 3). Progesterone levels started to decline on Day 13 and fell to less than 5 ng/ml on the day prior to oestrus. These studies make it unlikely that the pre-ovulatory secretion of oestradiol is responsible for the release of PGF which initiates luteal regression. 441. Significance of oestrogens in relation to prostaglandin F changes at parturition in the sheep Cox, R. I., WONG, M., CURRIE, W. B. and THOR-
BURN, G. D., C.S.I.R.O. Division of Animal Physiology, P.O. Box 239, Blacktown 2148, Australia The investigation was aimed at clarifying the role of oestrogen and prostaglandin changes in the initiation of parturition in the sheep. Blood samples were taken through cannulae chronically inserted in foetal carotid arteries or maternal utero-ovarian veins. Analyses were by competitive protein binding or radioimmunoassays. Measurement of maxima in E28> Ezw, El and their sulphates indicated concentrations at about 125 days of gestation as well as at parturition. Infusion of physiological amounts of E2fi into the ewe or of some potential oestrogen precursors into the foetus in late pregnancy led to an increase in PGF in the utero-ovarian vein and premature parturition. Premature parturition induced by corticosteroids infused into the foetus also resulted in both oestrogen and then PGF concentrations increasing. Analysis of foetal plasma for possible oestrogen precursors indicated that the classical Cl9 compounds were virtually absent. Some possible alternative intermediates were detected. The data are consistent with oestrogen from the conceptus stimulating uterine PGF biosynthesis or release, probably after transfer of the oestrogen to the maternal compartment. 442. Effect of prostaglandin F2a on gravid human uterine
401
three sub-groups! Molar-pregnancy (8 cases), fetal death (6 cases) and anencephaly (2 cases), subdividing these into two categories: Nuliparas and multiparas. Prostaglandin F2a was administered through amniotic puncture because this is one of the less explored routes to induce labor. A sample of patients with molar pregnancy, fetal death or anencephaly was chosen because the effect of prostaglandins on the product is unknown., Doses used (5 to 200 pg) were low compared to those given by others, since response determinesuterine reactivity. Prostaglandin was prepared in ethanol solution and there was no effect of this product on uterine contractivity. We feel it is better to start stimulation of uterine contractivity with low doses and increase them gradually according to response obtained, than starting with high doses. Dose-response curves were obtained showing the doses of prostaglandin versus the mean values of tonus, intensity, frequency and uterine activity. It was found that these four parameters increased when the dose of prostaglandin was augmented. In all three groups studied the uterine activity, cervical efacement and dilation were calculated. There was no significant changes in blood pressure readings during the first hours of the study, whereas the final part of the tracings showed an increase of differential due to elevation of the systolic component. Patients with fetal death had the cervical dilatiofi registered and the mean time required to dilate from 2 to IOcm was 9.50 h. Side effects observed were nausea, vomiting and chills. One patient had hypotension. Four patients had complications such as: Trophoblastic pulmonary embolism (one possible case), bleeding and hypotension and deciduo-myometritis. The remaining patients had no complications during the 2nd and 3rd phases of labor. Prostaglandin given by amniotic puncture caused few and mild side-effects. 443. Serum progesterone, human chorionic somatomammotropin, estriol and cortisol following intra- and extraamniotic administration of prostaglandin
RUNNEBAUM, B.. GERHARD,I., HENNER,H.. VEC’SEI. P., HALLER,U., R~~TTGERS, H. and KUBLI. F., Universitlts-Frauenklinik, Heidelberg, West Germany The present investigation was undertaken to evaluate changes in feto-placental and maternal hormone secretion following intra- and extra-amniotic injection of prostaglandin for induction of abortion. Before and after the administration of prostaglandin serial determinatiops of progesterone, human chorionic somatomammotropin, estriol and cortisol were performed by radioimmunoassay in the same serum samples during the induction-abortion-interval. In 13 pregnant women (week 14-20) induction of abortion was carried out by intra-amniotic installation of PGF!, and in 12 pregnant women (week 14-23) by intra-amniotic instillation of PGE,. In another group of pregnant women (week 11-16) PGF2, was applied by extra-amniotic injection for induction of abortion. The changes in the hormone levels during the inductionabortion interval are compared with the mode of uterine activity after intra- and extra-amniotic administration of the prostaglandins.
contractility
ROMERO-SALINAS, G., RUIZ-VELASCO,V., RAMIREZ- 444. Kinetic study of the action of gonadotropins and prostaghmdhm on oestrogen bioqkhesis, glycogenJIMENEZ,D., GARCIA-Pti~, J. and BRAVO-SANDOphosuhorvlase and cyclic AMP levels in human fullVAL, J., Department of Obstetric Physiology, Hospital ~erm~ph&ntas perfused “in vitro” de Gineco-Obstetricia No. 3, Centro Medico La Raza, ALSAT,E., LEVILLIEXS, J., AUGUY,A. and CEDARD,L., I.M.S.S., Mexico, D.F., Mexico City Facultt de MCdecine Cochin-Port-Royal (E.R. CNRS 123, U 35 INSERM) Paris, France A sample group of 16 patients was studied, divided into