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4461833 Chromatographically purifying proteolytic procoagulant enzyme from animal tissue extract
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4461833 CHROMATOGRAPHICALLY PURIFYING PROTEOLYTIC PROCOAGULANT ENZYME FROM ANIMAL TISSUE EXTRACT Stuart Gordon assigned to University Patents lnc A chromatographic procedure for the purification of a proteolytic procoagulant enzyme from extracts of human and animal tumors. The extracts are sequentially contacted with a first benzamide affinity chromatographic resin, an agarose filtration gel. a second benzamide affinity chromatographic resin and a phenylsepharose hydrophobic chromatographic resin. The resulting enzyme is capable of producing anti-procoagulant antibody which, which used in an immunoassay, is diagnostic for malignancy.
4463O9O CASCADE AMPLIFICATION ENZYME IMMUNOASSAY Curtis C Harris The invention provides enzyme immunoassays whose sensitivity is increased by cascade amplification. The coupled ligand (enzyme or an activatorl catalytically activates a second enzyme which acts on a substrate or can act on a third enzyme to produce a cascade. Alternatively. a proenzyme is coupled to the ligand and converted by an activator to an enzyme which is itself an activator of a second proenzyme in a cascade.
4467031 ENZYME-IMMUNOASSAY FOR CARCINOEMBRYONIC ANTIGEN Harald Gallati, Hans Brodbeck, Dornach, Switzerland assigned to Hoffmann-La Roche lnc A solid phase-sandwich enzyme-immunoassay method for the rapid determination of carcinoembryonic antigen (CEA). The sample to be investigated is incubated with a first CEA antibody, which is bound to a water-insoluble carrier, and a second CEA antibody, to which
peroxidase is bound directly or via a biotin avidin bridge. The immuno!ogical reaction can be carried out in one or two steps. By the presence of 0.4-1.0 mol I of phosphate ions, 0.3-0.4 mol '1 of sulfate ions or 0.2-0.4 mol I of tartrate ions in the immunological reaction or m the second step thereof(insofar as it ~scarried out in two steps) the incubation time period is shortened considerably. After the immunological reaction, the phases are separated, whereupon the peroxidase activity is measured either in the solid or in the liquid phase as the amount of CEA present in the sample.
4469789 AMINO ACID AND PEPTIDE ESTERS OF LEUKOINDOANILINE COMPOUNDS AND COMPOSITIONS FOR THE DETECTION OF PROTEOLYTIC ENZYMES Dieter Berger, Franz Braun, Gunter Fre.~. Wolfgang-Reinhold Knappe. Manfred Kuhr, Wolfgang Werner, Viernheim, Federal Republic Of Germany assigned to Boehringer Mannheim GmbH This invention relates to new amino acid and peptide esters of leuko-indoaniline compounds and to a process for their preparation. In additional aspect, the invention relates to compositions containing such compounds for the detection of proteolytic enzymes and to methods for detecting such enzymes.
4469791 GENETICALLY ENGINEERED MICROORGANISMS FOR MASSIVE PRODUCTION OF AMYLOLYTIC ENZYMES AND PROCESS FOR PREPARING SAME Charles A Colson, Pierre E Cornelis. Colette S Digneffe, Corinne Walon, Dion Valmont, Belgium assigned to CPC International Inc Genetically engineered microorganisms are provided which contain recombinant DNA with an amylase coding gene. Improved yields of amylase enzymes are obtained by cultivating these microorganisms.
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4461833 CHROMATOGRAPHICALLY PURIFYING PROTEOLYTIC PROCOAGULANT ENZYME FROM ANIMAL TISSUE EXTRACT Stuart Gordon assigned to University Patents lnc A chromatographic procedure for the purification of a proteolytic procoagulant enzyme from extracts of human and animal tumors. The extracts are sequentially contacted with a first benzamide affinity chromatographic resin, an agarose filtration gel. a second benzamide affinity chromatographic resin and a phenylsepharose hydrophobic chromatographic resin. The resulting enzyme is capable of producing anti-procoagulant antibody which, which used in an immunoassay, is diagnostic for malignancy.
4463O9O CASCADE AMPLIFICATION ENZYME IMMUNOASSAY Curtis C Harris The invention provides enzyme immunoassays whose sensitivity is increased by cascade amplification. The coupled ligand (enzyme or an activatorl catalytically activates a second enzyme which acts on a substrate or can act on a third enzyme to produce a cascade. Alternatively. a proenzyme is coupled to the ligand and converted by an activator to an enzyme which is itself an activator of a second proenzyme in a cascade.
4467031 ENZYME-IMMUNOASSAY FOR CARCINOEMBRYONIC ANTIGEN Harald Gallati, Hans Brodbeck, Dornach, Switzerland assigned to Hoffmann-La Roche lnc A solid phase-sandwich enzyme-immunoassay method for the rapid determination of carcinoembryonic antigen (CEA). The sample to be investigated is incubated with a first CEA antibody, which is bound to a water-insoluble carrier, and a second CEA antibody, to which
peroxidase is bound directly or via a biotin avidin bridge. The immuno!ogical reaction can be carried out in one or two steps. By the presence of 0.4-1.0 mol I of phosphate ions, 0.3-0.4 mol '1 of sulfate ions or 0.2-0.4 mol I of tartrate ions in the immunological reaction or m the second step thereof(insofar as it ~scarried out in two steps) the incubation time period is shortened considerably. After the immunological reaction, the phases are separated, whereupon the peroxidase activity is measured either in the solid or in the liquid phase as the amount of CEA present in the sample.
4469789 AMINO ACID AND PEPTIDE ESTERS OF LEUKOINDOANILINE COMPOUNDS AND COMPOSITIONS FOR THE DETECTION OF PROTEOLYTIC ENZYMES Dieter Berger, Franz Braun, Gunter Fre.~. Wolfgang-Reinhold Knappe. Manfred Kuhr, Wolfgang Werner, Viernheim, Federal Republic Of Germany assigned to Boehringer Mannheim GmbH This invention relates to new amino acid and peptide esters of leuko-indoaniline compounds and to a process for their preparation. In additional aspect, the invention relates to compositions containing such compounds for the detection of proteolytic enzymes and to methods for detecting such enzymes.
4469791 GENETICALLY ENGINEERED MICROORGANISMS FOR MASSIVE PRODUCTION OF AMYLOLYTIC ENZYMES AND PROCESS FOR PREPARING SAME Charles A Colson, Pierre E Cornelis. Colette S Digneffe, Corinne Walon, Dion Valmont, Belgium assigned to CPC International Inc Genetically engineered microorganisms are provided which contain recombinant DNA with an amylase coding gene. Improved yields of amylase enzymes are obtained by cultivating these microorganisms.