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4482482 Cesium trifluoroacetate solutions for the isolation of nucleic acids
PATENT ABSTRACTS
86
°~
Small cosmid cloning vectors having two antibiotic resistance markers, selected from chloramphemicol resistance, streptomycin and spectinomycin resistance and tetracycline resistance markers, at least one having a unique restriction site.
Chimeric plasmids capable of transforming bacteria and yeast are described. The plasmids carry the Cm (chloramphenicol resistance) gene and the Tc (tetracycline resistance) gene as selectable markers. The Cm gene allows the plasmids to be selected for in wild-type strains of the yeast Saccharomyces cerevisiae. The Tc gene allows heterologous genes cloned into the plasmids to be selected for in Escherichia colt bacteria.
4478937 PLASMID
4477569 PENTOSE FERMENTATION SELECTED YEAST
WITH
AND PRODUCTION THEREOF
Masakazu Kikuchi, Takaki Hayakawa, Makoto Kida, Toyono gun, Osaka, Japan
Henr Schneider, Ryszard Maleszka, Patrick Y Wang, Ivan A Veliky, Yui-Kwok Chan, Nepean, Canada assigned to Canadian Patents & Development Limited
if,J
It has been found possible to ferment the pentose D-xylose directly to ethanol with the selected yeast Pachysolen tannophilus. At least an initial aerobic stage is desirable. Improved yields have been obtained when the yeast cells are recycled continually at high cell densities. Fermentation of D-galactose and other hexoses along with Dxylose has been found possible with selected strains or mutants.
CHIMERIC PLASMIDS THAT REPLICATE IN BACTERIA AND YEAST AND MICROORGANISMS TRANSFORMED THEREWITH Shing Chang, James H Meade assigned to Cetus Corporation
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N u m b e r o f Cleavage Sites 2 I
1 ~3 1 1 ~ 3
A novel plasmid pATM2 is obtainable from a microorganism belonging to the genus Micromonospora. The plasmid pATM2 is useful as a cloning vector in recombinant DNA work.
4482482
4477571
o I[ (-~
Restriction cndonuclease Bam H I Bgl I1 Kpn ] Sal 1 Eco R I Hind llI Sma I
/ /I /I r' .~=
,'/
CESIUM TRIFLUOROACETATE SOLUTIONS FOR THE ISOLATION OF NUCLEIC ACIDS Lawrence A Haft, Anthony J Ewell assigned to Pharmacia Inc A method for simultaneously isolating DNA, RNA and/or protein fractions from a crude biological mixture containing DNA, RNA and/or protein fractions is disclosed. The method comprises adding the crude biological mixture to an aqueous solution of cesium trifluoroacetate, the solution having a density from about 1.4 to about 1.6 g/ml, and centrifuging the resulting mixture under ultracentrifugation conditions.
86
°~
Small cosmid cloning vectors having two antibiotic resistance markers, selected from chloramphemicol resistance, streptomycin and spectinomycin resistance and tetracycline resistance markers, at least one having a unique restriction site.
Chimeric plasmids capable of transforming bacteria and yeast are described. The plasmids carry the Cm (chloramphenicol resistance) gene and the Tc (tetracycline resistance) gene as selectable markers. The Cm gene allows the plasmids to be selected for in wild-type strains of the yeast Saccharomyces cerevisiae. The Tc gene allows heterologous genes cloned into the plasmids to be selected for in Escherichia colt bacteria.
4478937 PLASMID
4477569 PENTOSE FERMENTATION SELECTED YEAST
WITH
AND PRODUCTION THEREOF
Masakazu Kikuchi, Takaki Hayakawa, Makoto Kida, Toyono gun, Osaka, Japan
Henr Schneider, Ryszard Maleszka, Patrick Y Wang, Ivan A Veliky, Yui-Kwok Chan, Nepean, Canada assigned to Canadian Patents & Development Limited
if,J
It has been found possible to ferment the pentose D-xylose directly to ethanol with the selected yeast Pachysolen tannophilus. At least an initial aerobic stage is desirable. Improved yields have been obtained when the yeast cells are recycled continually at high cell densities. Fermentation of D-galactose and other hexoses along with Dxylose has been found possible with selected strains or mutants.
CHIMERIC PLASMIDS THAT REPLICATE IN BACTERIA AND YEAST AND MICROORGANISMS TRANSFORMED THEREWITH Shing Chang, James H Meade assigned to Cetus Corporation
, ,vm~ ....
. . . . \~:o> \ ~ , ~ : : ~
N u m b e r o f Cleavage Sites 2 I
1 ~3 1 1 ~ 3
A novel plasmid pATM2 is obtainable from a microorganism belonging to the genus Micromonospora. The plasmid pATM2 is useful as a cloning vector in recombinant DNA work.
4482482
4477571
o I[ (-~
Restriction cndonuclease Bam H I Bgl I1 Kpn ] Sal 1 Eco R I Hind llI Sma I
/ /I /I r' .~=
,'/
CESIUM TRIFLUOROACETATE SOLUTIONS FOR THE ISOLATION OF NUCLEIC ACIDS Lawrence A Haft, Anthony J Ewell assigned to Pharmacia Inc A method for simultaneously isolating DNA, RNA and/or protein fractions from a crude biological mixture containing DNA, RNA and/or protein fractions is disclosed. The method comprises adding the crude biological mixture to an aqueous solution of cesium trifluoroacetate, the solution having a density from about 1.4 to about 1.6 g/ml, and centrifuging the resulting mixture under ultracentrifugation conditions.