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4483922 Inactivation of enzymes
106
PATENT ABSTRACTS
of being split offby enzymatic hydrolysis to form a colored or fluorescent product H-R5. The quantity of split product H-R5 released by the enzymatic action on the tripeptide derivative is determined photometrically, spectrophotometrically, fluorescencespectrophotometrically, or electrochemically. The quantity of released split product H-R5 per time unit is proportional to the quantity of enzyme present in the starting material.
4480036 PROTEOLYTIC
Enzyme particles are coated or at least partially coated with a salt of a lower alkyl siliconic acid and/or polymerization product(s) thereof which may be formed from such acid on storage. The coated enzyme particles are useful as components of heavy duty fabric softening laundry detergent compositions, such as those which contain bentonite to soften laundry. The coating material, which may be more simply referred to as the siliconate, assists in stabilizing the enzyme when such is incorporated in detergent compositions, helps to prevent adherence of the coated particles to container or compartment walls and can aid in controlling the foaming activity of the built synthetic organic detergents.
ENZYME
Hugh W Morgan, Roy M Daniel, Donald A Cowan, Christopher W Hickey, Hamilton, New Zealand assigned to Development Finance Corporation
4483922 INACTIVATION OF ENZYMES
The invention relates to an extremely thermophilic bacterium, Thermus T-351, and a thermophilic protease, Caldolysin, derived therefrom. The bacterium cells are Gram negative, nonsporulating rods. Its natural environment is a hot pool at 79 degrees+/-4 degrees C. The protease is stable at temperatures up to 75 degrees C. at a pH range of 4 to 12. It is most active at temperatures of 65 degrees C. to 85 degrees C. but retains at least some activity at lower temperatures.
4481294
Charles R Carpenter, Robert H Dodge, Kenneth A Rosanoff assigned to AMF Inc A method for inactivating enzymes, particularly enzymes found in human serum. The method comprises reacting the enzyme in a suitable medium, e.g. human serum, with an inactivating amount of peracetic acid. In the preferred embodiment the treated serum is subsequently neutralized and dialyzed. Preferably, the serums are used as standards for calibration or as a control in assay kits, e.g. R1A, and EIA or enzyme kits, for the determination of specific ligands and enzymes.
PROCESS FOR CELL DISRUPTION John D Downs, Sittingbourne, United Kingdom assigned to Shell Oil Company Process for disrupting cells by contacting an aqueous, cell-containing medium with a protease enzyme, wherein the enzymic contact is preceded by contact with an ionic surfactant; the polysaccharide solutions thereby produced; and a process for displacing a fluid through a well and/or a permeable subsurface formation communicating with the well, by injecting into the well an optionally diluted, aqueous solution of such a polysaccharide.
4482630 SILICONATE-COATED
ENZYME
Edwin Allen, Alan Dillarstone, Joseph A Reul, Oupeye, Belgium assigned to Colgate-Palmolive Company
4486534 P R O C E S S FOR THE PREPARATION OF IMMUNOLOGICALLY ACTIVE ENZYME TAGGED CONJUGATES Winfrie Albert, Helmut Lenz, Pfederal Republic Of Germana assigned to Boehringer Mannheim GmbH A process for the preparation of immunologically active tagged conjugates by covalent linking of an enzyme with an antigen or haptene, which process comprises placing a solution of a mixture of immunologically active and inactive conjugates in contact with an insoluble complex former able to form a non-immunological complex reversibly with the untagged antigen or haptene component of the conjugate, separating the insoluble complex former, and eluting with a desorbent for untagged antigen or baptene.
PATENT ABSTRACTS
of being split offby enzymatic hydrolysis to form a colored or fluorescent product H-R5. The quantity of split product H-R5 released by the enzymatic action on the tripeptide derivative is determined photometrically, spectrophotometrically, fluorescencespectrophotometrically, or electrochemically. The quantity of released split product H-R5 per time unit is proportional to the quantity of enzyme present in the starting material.
4480036 PROTEOLYTIC
Enzyme particles are coated or at least partially coated with a salt of a lower alkyl siliconic acid and/or polymerization product(s) thereof which may be formed from such acid on storage. The coated enzyme particles are useful as components of heavy duty fabric softening laundry detergent compositions, such as those which contain bentonite to soften laundry. The coating material, which may be more simply referred to as the siliconate, assists in stabilizing the enzyme when such is incorporated in detergent compositions, helps to prevent adherence of the coated particles to container or compartment walls and can aid in controlling the foaming activity of the built synthetic organic detergents.
ENZYME
Hugh W Morgan, Roy M Daniel, Donald A Cowan, Christopher W Hickey, Hamilton, New Zealand assigned to Development Finance Corporation
4483922 INACTIVATION OF ENZYMES
The invention relates to an extremely thermophilic bacterium, Thermus T-351, and a thermophilic protease, Caldolysin, derived therefrom. The bacterium cells are Gram negative, nonsporulating rods. Its natural environment is a hot pool at 79 degrees+/-4 degrees C. The protease is stable at temperatures up to 75 degrees C. at a pH range of 4 to 12. It is most active at temperatures of 65 degrees C. to 85 degrees C. but retains at least some activity at lower temperatures.
4481294
Charles R Carpenter, Robert H Dodge, Kenneth A Rosanoff assigned to AMF Inc A method for inactivating enzymes, particularly enzymes found in human serum. The method comprises reacting the enzyme in a suitable medium, e.g. human serum, with an inactivating amount of peracetic acid. In the preferred embodiment the treated serum is subsequently neutralized and dialyzed. Preferably, the serums are used as standards for calibration or as a control in assay kits, e.g. R1A, and EIA or enzyme kits, for the determination of specific ligands and enzymes.
PROCESS FOR CELL DISRUPTION John D Downs, Sittingbourne, United Kingdom assigned to Shell Oil Company Process for disrupting cells by contacting an aqueous, cell-containing medium with a protease enzyme, wherein the enzymic contact is preceded by contact with an ionic surfactant; the polysaccharide solutions thereby produced; and a process for displacing a fluid through a well and/or a permeable subsurface formation communicating with the well, by injecting into the well an optionally diluted, aqueous solution of such a polysaccharide.
4482630 SILICONATE-COATED
ENZYME
Edwin Allen, Alan Dillarstone, Joseph A Reul, Oupeye, Belgium assigned to Colgate-Palmolive Company
4486534 P R O C E S S FOR THE PREPARATION OF IMMUNOLOGICALLY ACTIVE ENZYME TAGGED CONJUGATES Winfrie Albert, Helmut Lenz, Pfederal Republic Of Germana assigned to Boehringer Mannheim GmbH A process for the preparation of immunologically active tagged conjugates by covalent linking of an enzyme with an antigen or haptene, which process comprises placing a solution of a mixture of immunologically active and inactive conjugates in contact with an insoluble complex former able to form a non-immunological complex reversibly with the untagged antigen or haptene component of the conjugate, separating the insoluble complex former, and eluting with a desorbent for untagged antigen or baptene.